UniProtKB - Q9JJS5 (SH3B4_RAT)
Protein
SH3 domain-binding protein 4
Gene
Sh3bp4
Organism
Rattus norvegicus (Rat)
Status
Functioni
May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy (By similarity).By similarity
GO - Molecular functioni
- GDP-dissociation inhibitor activity Source: UniProtKB
- identical protein binding Source: RGD
- Ras GTPase binding Source: RGD
GO - Biological processi
- cellular response to amino acid stimulus Source: UniProtKB
- endocytosis Source: UniProtKB-KW
- negative regulation of cell growth Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of GTPase activity Source: UniProtKB
- negative regulation of TOR signaling Source: UniProtKB
- positive regulation of autophagy Source: UniProtKB
- protein localization to lysosome Source: UniProtKB
- regulation of catalytic activity Source: UniProtKB
Keywordsi
Biological process | Endocytosis |
Enzyme and pathway databases
Reactomei | R-RNO-9639288, Amino acids regulate mTORC1 |
Names & Taxonomyi
Protein namesi | Recommended name: SH3 domain-binding protein 4 |
Gene namesi | Name:Sh3bp4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620219, Sh3bp4 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- clathrin-coated pit By similarity
- clathrin-coated vesicle By similarity
Note: Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus (By similarity).By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
Other locations
- clathrin-coated vesicle Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Coated pit, Cytoplasmic vesicle, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274576 | 1 – 961 | SH3 domain-binding protein 4Add BLAST | 961 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 131 | PhosphoserineCombined sources | 1 | |
Modified residuei | 244 | PhosphoserineCombined sources | 1 | |
Modified residuei | 249 | PhosphoserineBy similarity | 1 | |
Modified residuei | 277 | PhosphoserineBy similarity | 1 | |
Modified residuei | 294 | PhosphoserineBy similarity | 1 | |
Modified residuei | 635 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2 (By similarity).By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9JJS5 |
PRIDEi | Q9JJS5 |
PTM databases
iPTMneti | Q9JJS5 |
PhosphoSitePlusi | Q9JJS5 |
Interactioni
Subunit structurei
Homodimer or homooligomer.
Interacts with DNM2, EPS15, clathrin, the adapter protein complex 2/AP-2 and TFRC.
Interacts with the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; the interaction is most probably direct, preferentially occurs with their inactive GDP-bound form and is negatively regulated by amino acids (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- Ras GTPase binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000026312 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 114 | SH3 1PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 315 – 452 | ZU5PROSITE-ProRule annotationAdd BLAST | 138 | |
Domaini | 652 – 722 | SH3 2PROSITE-ProRule annotationAdd BLAST | 71 |
Domaini
The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity. The SH3 domain also mediates interaction with RRAGB, RRAGC and is required for the negative regulation of mTORC1 (By similarity).By similarity
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
eggNOGi | ENOG502QTUW, Eukaryota |
InParanoidi | Q9JJS5 |
PhylomeDBi | Q9JJS5 |
Family and domain databases
CDDi | cd11757, SH3_SH3BP4, 1 hit |
InterProi | View protein in InterPro IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR035455, SH3BP4 IPR035456, SH3BP4_SH3 IPR000906, ZU5_dom |
PANTHERi | PTHR15603:SF3, PTHR15603:SF3, 1 hit |
Pfami | View protein in Pfam PF00018, SH3_1, 1 hit PF07653, SH3_2, 1 hit PF00791, ZU5, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 2 hits PS51145, ZU5, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9JJS5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAQRIRAAN SSGLPRCKSE GTLIDLSEGF SETSFNDVKV PSPSALLVDN
60 70 80 90 100
PTPFGNAKEV IAIKDYCPNN FTTLKFSKGD HLYVLDTSGG EWWYAHNTTE
110 120 130 140 150
MGYIPSSYVQ PLNYRNSTLS DSGMIDNLPD SPDEVAKELD LLGGWTDDQK
160 170 180 190 200
QSGRPYSNNP FWNGVRTNPF LNGNAQPSMD ELNPKSTVDL LLFDTGTSSF
210 220 230 240 250
TESSSATTNS TGNIFDELPA TNGIHLEQPV KRDNPFFRSK RSYSLSELSV
260 270 280 290 300
LQAKSDAPPT SSFFTGLKSP APEQFQSRED FRTAWLNHRK LARSCHDLDL
310 320 330 340 350
LGQSPGWGQT QAVETNIVCK LDSSGGSVQL PDTSISIHVP EGHVAPGETQ
360 370 380 390 400
QISMKALLDP PLDLNSDRST SISPVVEVKL SNLEVSTFII LEMKVSAEVK
410 420 430 440 450
GDIFSKSTVV LQCLRSDSKE GPYAPIPLAY SYGDTIQVQL DNLEPCMYLA
460 470 480 490 500
IVAQGSNILY PSTVWDFIHK RVTVGLYGPK HIHPSFKTVV TIFGHDCAPK
510 520 530 540 550
TLLVTEVTRQ APSPAPVALQ LWGKHQFILS RPQDLRVCMF SNMTNYDVKS
560 570 580 590 600
NEQAKVVRGF QMKLGKVSRL IFSVISQNPN ELSDFTLRVQ VKDDQDTILT
610 620 630 640 650
QFCVQTPQPP PKSAIKPSGQ RRFLKKNEVG KIILSPFVVT TKYPTFQDRP
660 670 680 690 700
VSSLKFGKLL KTVVRQNKSH YLLEYKKGDV VALLSEERIR LKGQLWTKEW
710 720 730 740 750
YIGYYQGKVG LVHTKNVLVV GKARPSLFSG PELSTSVLLE QILRPCKFLT
760 770 780 790 800
YIYASVRTLL MENISSWRAF ADALGYGNLP LTFFCRAELD SEPERVASVL
810 820 830 840 850
EKLKEDCNNP DNKDRKSFQK ELVMALLKMD CQGLVVRLIQ DFVLLTTAVE
860 870 880 890 900
VAQRWRELAE KLAKVSKQQM DAYESPHRDR NGVVDSEAMW KPAYDFLLTW
910 920 930 940 950
SHQIGDSYRD VIQELHIGLD KMKNPITRRW KHLTGTLILV NSLDILRAAA
960
FSPADHDDFV I
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V8J0 | G3V8J0_RAT | SH3 domain-binding protein 4 | Sh3bp4 rCG_55462 | 961 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278266 mRNA Translation: CAB93353.2 |
Genome annotation databases
UCSCi | RGD:620219, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278266 mRNA Translation: CAB93353.2 |
3D structure databases
SMRi | Q9JJS5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000026312 |
PTM databases
iPTMneti | Q9JJS5 |
PhosphoSitePlusi | Q9JJS5 |
Proteomic databases
PaxDbi | Q9JJS5 |
PRIDEi | Q9JJS5 |
Genome annotation databases
UCSCi | RGD:620219, rat |
Organism-specific databases
RGDi | 620219, Sh3bp4 |
Phylogenomic databases
eggNOGi | ENOG502QTUW, Eukaryota |
InParanoidi | Q9JJS5 |
PhylomeDBi | Q9JJS5 |
Enzyme and pathway databases
Reactomei | R-RNO-9639288, Amino acids regulate mTORC1 |
Miscellaneous databases
PROi | PR:Q9JJS5 |
Family and domain databases
CDDi | cd11757, SH3_SH3BP4, 1 hit |
InterProi | View protein in InterPro IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR035455, SH3BP4 IPR035456, SH3BP4_SH3 IPR000906, ZU5_dom |
PANTHERi | PTHR15603:SF3, PTHR15603:SF3, 1 hit |
Pfami | View protein in Pfam PF00018, SH3_1, 1 hit PF07653, SH3_2, 1 hit PF00791, ZU5, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 2 hits PS51145, ZU5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SH3B4_RAT | |
Accessioni | Q9JJS5Primary (citable) accession number: Q9JJS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2007 |
Last sequence update: | March 1, 2004 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |