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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

Gene

Pfkfb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Activity regulationi

The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81Fructose 6-phosphateBy similarity1
Binding sitei105Fructose 6-phosphateBy similarity1
Active sitei131Sequence analysis1
Binding sitei133Fructose 6-phosphateBy similarity1
Binding sitei139Fructose 6-phosphateBy similarity1
Active sitei161Sequence analysis1
Binding sitei175Fructose 6-phosphateBy similarity1
Binding sitei196Fructose 6-phosphateBy similarity1
Binding sitei200Fructose 6-phosphateBy similarity1
Binding sitei259Fructose 2,6-bisphosphateBy similarity1
Sitei259Transition state stabilizerBy similarity1
Active sitei260Tele-phosphohistidine intermediateBy similarity1
Binding sitei266Fructose 2,6-bisphosphateBy similarity1
Sitei266Transition state stabilizerBy similarity1
Binding sitei272Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Active sitei329Proton donor/acceptorBy similarity1
Binding sitei340Fructose 2,6-bisphosphateBy similarity1
Binding sitei354Fructose 2,6-bisphosphateBy similarity1
Binding sitei358Fructose 2,6-bisphosphateBy similarity1
Binding sitei369Fructose 2,6-bisphosphateBy similarity1
Sitei394Transition state stabilizerBy similarity1
Binding sitei395Fructose 2,6-bisphosphateBy similarity1
Binding sitei399Fructose 2,6-bisphosphateBy similarity1
Binding sitei431ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 56ATPBy similarity9
Nucleotide bindingi170 – 175ATPBy similarity6
Nucleotide bindingi351 – 354ATPBy similarity4
Nucleotide bindingi395 – 399ATPBy similarity5

GO - Molecular functioni

  • 6-phosphofructo-2-kinase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • fructose-2,6-bisphosphate 2-phosphatase activity Source: CACAO
  • kinase binding Source: RGD
  • protein kinase binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Multifunctional enzyme, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.105 5301
3.1.3.46 5301
ReactomeiR-RNO-70171 Glycolysis
SABIO-RKiQ9JJH5

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
Short name:
6PF-2-K/Fru-2,6-P2ase 2
Short name:
PFK/FBPase 2
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase heart-type isozyme
RH2K
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:Pfkfb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi3309 Pfkfb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001799662 – 5576-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2Add BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei32Phosphoserine; by PKABy similarity1
Modified residuei469PhosphoserineCombined sources1
Modified residuei471PhosphothreonineBy similarity1
Modified residuei478Phosphothreonine; by PKCBy similarity1
Modified residuei486PhosphoserineCombined sources1
Modified residuei496PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by AMPK stimulates activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JJH5
PRIDEiQ9JJH5

PTM databases

iPTMnetiQ9JJH5
PhosphoSitePlusiQ9JJH5

Expressioni

Gene expression databases

BgeeiENSRNOG00000004162 Expressed in 10 organ(s), highest expression level in heart
ExpressionAtlasiQ9JJH5 baseline and differential
GenevisibleiQ9JJH5 RN

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-2041 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex
STRINGi10116.ENSRNOP00000005729

Structurei

3D structure databases

ProteinModelPortaliQ9JJH5
SMRiQ9JJH5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 2516-phosphofructo-2-kinaseAdd BLAST250
Regioni252 – 557Fructose-2,6-bisphosphataseAdd BLAST306

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiKOG0234 Eukaryota
COG0406 LUCA
GeneTreeiENSGT00390000018751
HOGENOMiHOG000181112
HOVERGENiHBG005628
InParanoidiQ9JJH5
KOiK19029
OMAiHDSCEWL
OrthoDBiEOG091G0A43
PhylomeDBiQ9JJH5
TreeFamiTF313541

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR003094 6Pfruct_kin
IPR013079 6Phosfructo_kin
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
IPR027417 P-loop_NTPase
IPR001345 PG/BPGM_mutase_AS
PANTHERiPTHR10606 PTHR10606, 1 hit
PfamiView protein in Pfam
PF01591 6PF2K, 1 hit
PF00300 His_Phos_1, 1 hit
PRINTSiPR00991 6PFRUCTKNASE
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00175 PG_MUTASE, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 3 (identifier: Q9JJH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSENSTFSTE DSSSSSYKPH ASNLRRAGKK CSWASYMTNS PTLIVMIGLP
60 70 80 90 100
ARGKTYVSKK LTRYLNWIGV PTKVFNLGVY RREAVKSYKS YDFFRHDNEE
110 120 130 140 150
AMKIRKQCAL VALEDVKAYF TEESGQIAVF DATNTTRERR DMILNFAKQN
160 170 180 190 200
AFKVFFVESV CDDPDVIAAN ILEVKVSSPD YPERNRENVM EDFLKRIECY
210 220 230 240 250
KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK IVYYLMNIHV
260 270 280 290 300
HPRTIYLCRH GESEFNLLGK IGGDSGLSLR GKQFAQALKK FLEEQEIQDL
310 320 330 340 350
KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE
360 370 380 390 400
FALRDQEKYL YRYPGGESYQ DLVQRLEPVI MELERQGNVL VISHQAVMRC
410 420 430 440 450
LLAYFLDKGA DELPYLRCPL HIIFKLTPVA YGCKVETITL NVEAVDTHRD
460 470 480 490 500
KPTHNFPKSQ TPVRMRRNSF TPLSSSNTIR RPRNYSVGSR PLKPLSPLRA
510 520 530 540 550
LDMQEGADQP KTQVQQGSAQ ATEHLQKALE FANGHREVEN VLAKHRRPSM

ASLTLLS
Length:557
Mass (Da):64,156
Last modified:October 1, 2000 - v1
Checksum:iD5707054E416629F
GO
Isoform 1 (identifier: Q9JJH5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     515-519: QQGSA → SIPVV
     520-547: Missing.

Show »
Length:529
Mass (Da):60,916
Checksum:i013AABF875C60840
GO
Isoform 2 (identifier: Q9JJH5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     514-536: Missing.

Show »
Length:534
Mass (Da):61,653
Checksum:i88C635DE3964CD53
GO
Isoform 4 (identifier: Q9JJH5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     454-536: Missing.

Show »
Length:474
Mass (Da):54,823
Checksum:i1EDFF196B219764D
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9DRP5C9DRP5_RAT
6-phosphofructo-2-kinase/fructose-2...
Pfkfb2 pfkfb2
274Annotation score:
R9PXY6R9PXY6_RAT
6-phosphofructo-2-kinase/fructose-2...
Pfkfb2 rCG_46560
519Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004678454 – 536Missing in isoform 4. CuratedAdd BLAST83
Alternative sequenceiVSP_004676514 – 536Missing in isoform 2. CuratedAdd BLAST23
Alternative sequenceiVSP_004677515 – 519QQGSA → SIPVV in isoform 1. Curated5
Alternative sequenceiVSP_004679520 – 547Missing in isoform 1. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67900 mRNA Translation: AAB29678.1
L27084 mRNA Translation: AAA41132.1
AB040530 mRNA Translation: BAA96495.1
AB040531 mRNA Translation: BAA96496.1
AB040532 mRNA Translation: BAA96497.1
AB040533 mRNA Translation: BAA96498.1
PIRiJC2037
RefSeqiNP_001029136.1, NM_001033964.2 [Q9JJH5-3]
NP_001029137.1, NM_001033965.2 [Q9JJH5-1]
NP_536725.2, NM_080477.3 [Q9JJH5-4]
XP_006249768.1, XM_006249706.3
XP_006249769.1, XM_006249707.3
XP_006249770.1, XM_006249708.1
XP_006249771.1, XM_006249709.3 [Q9JJH5-4]
XP_006249773.1, XM_006249711.3 [Q9JJH5-1]
UniGeneiRn.44844

Genome annotation databases

EnsembliENSRNOT00000005729; ENSRNOP00000005729; ENSRNOG00000004162 [Q9JJH5-1]
ENSRNOT00000037679; ENSRNOP00000030819; ENSRNOG00000004162 [Q9JJH5-4]
ENSRNOT00000050354; ENSRNOP00000044506; ENSRNOG00000004162 [Q9JJH5-3]
GeneIDi24640
KEGGirno:24640
UCSCiRGD:3309 rat [Q9JJH5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S67900 mRNA Translation: AAB29678.1
L27084 mRNA Translation: AAA41132.1
AB040530 mRNA Translation: BAA96495.1
AB040531 mRNA Translation: BAA96496.1
AB040532 mRNA Translation: BAA96497.1
AB040533 mRNA Translation: BAA96498.1
PIRiJC2037
RefSeqiNP_001029136.1, NM_001033964.2 [Q9JJH5-3]
NP_001029137.1, NM_001033965.2 [Q9JJH5-1]
NP_536725.2, NM_080477.3 [Q9JJH5-4]
XP_006249768.1, XM_006249706.3
XP_006249769.1, XM_006249707.3
XP_006249770.1, XM_006249708.1
XP_006249771.1, XM_006249709.3 [Q9JJH5-4]
XP_006249773.1, XM_006249711.3 [Q9JJH5-1]
UniGeneiRn.44844

3D structure databases

ProteinModelPortaliQ9JJH5
SMRiQ9JJH5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-2041 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex
STRINGi10116.ENSRNOP00000005729

PTM databases

iPTMnetiQ9JJH5
PhosphoSitePlusiQ9JJH5

Proteomic databases

PaxDbiQ9JJH5
PRIDEiQ9JJH5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005729; ENSRNOP00000005729; ENSRNOG00000004162 [Q9JJH5-1]
ENSRNOT00000037679; ENSRNOP00000030819; ENSRNOG00000004162 [Q9JJH5-4]
ENSRNOT00000050354; ENSRNOP00000044506; ENSRNOG00000004162 [Q9JJH5-3]
GeneIDi24640
KEGGirno:24640
UCSCiRGD:3309 rat [Q9JJH5-1]

Organism-specific databases

CTDi5208
RGDi3309 Pfkfb2

Phylogenomic databases

eggNOGiKOG0234 Eukaryota
COG0406 LUCA
GeneTreeiENSGT00390000018751
HOGENOMiHOG000181112
HOVERGENiHBG005628
InParanoidiQ9JJH5
KOiK19029
OMAiHDSCEWL
OrthoDBiEOG091G0A43
PhylomeDBiQ9JJH5
TreeFamiTF313541

Enzyme and pathway databases

BRENDAi2.7.1.105 5301
3.1.3.46 5301
ReactomeiR-RNO-70171 Glycolysis
SABIO-RKiQ9JJH5

Miscellaneous databases

PROiPR:Q9JJH5

Gene expression databases

BgeeiENSRNOG00000004162 Expressed in 10 organ(s), highest expression level in heart
ExpressionAtlasiQ9JJH5 baseline and differential
GenevisibleiQ9JJH5 RN

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR003094 6Pfruct_kin
IPR013079 6Phosfructo_kin
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
IPR027417 P-loop_NTPase
IPR001345 PG/BPGM_mutase_AS
PANTHERiPTHR10606 PTHR10606, 1 hit
PfamiView protein in Pfam
PF01591 6PF2K, 1 hit
PF00300 His_Phos_1, 1 hit
PRINTSiPR00991 6PFRUCTKNASE
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00175 PG_MUTASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiF262_RAT
AccessioniPrimary (citable) accession number: Q9JJH5
Secondary accession number(s): Q64297, Q9JHL5, Q9JJH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 1, 2000
Last modified: September 12, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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