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Protein

Ribosomal oxygenase 1

Gene

Riox1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation (By similarity). Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2).By similarity1 Publication

Catalytic activityi

L-histidine-[60S ribosomal protein L8] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L8] + succinate + CO2.1 Publication
Protein N6,N6-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O2 = protein L-lysine + 2 succinate + 2 formaldehyde + 2 CO2.1 Publication

Cofactori

Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi302Iron; catalyticCurated1
Metal bindingi304Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi367Iron; catalyticCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Dioxygenase, Oxidoreductase, Repressor
Biological processTranscription, Transcription regulation
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal oxygenase 1Imported
Alternative name(s):
Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 (EC:1.14.11.-, EC:1.14.11.271 Publication)
Histone lysine demethylase NO66
Gene namesi
Name:Riox1Imported
Synonyms:Mapjd, No66
ORF Names:MNCb-7109
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1919202 Riox1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi302H → A: Loss of demethylase activity. 1 Publication1
Mutagenesisi367H → A: Loss of demethylase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002646141 – 603Ribosomal oxygenase 1Add BLAST603

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei65PhosphoserineBy similarity1
Modified residuei68PhosphoserineBy similarity1
Modified residuei91PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JJF3
MaxQBiQ9JJF3
PaxDbiQ9JJF3
PeptideAtlasiQ9JJF3
PRIDEiQ9JJF3

PTM databases

iPTMnetiQ9JJF3
PhosphoSitePlusiQ9JJF3

Expressioni

Tissue specificityi

Present in developing bones (at protein level). Widely but not ubiquitously expressed.

Gene expression databases

BgeeiENSMUSG00000046791 Expressed in 245 organ(s), highest expression level in blastocyst
CleanExiMM_2410016O06RIK
GenevisibleiQ9JJF3 MM

Interactioni

Subunit structurei

Interacts with SP7/OSX; the interaction is direct. Interacts with PHF19; leading to its recruitment to H3K36me3 sites. Interacts with MYC (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Sp7Q8VI673EBI-7608809,EBI-7608836

Protein-protein interaction databases

BioGridi215052, 2 interactors
IntActiQ9JJF3, 4 interactors
MINTiQ9JJF3
STRINGi10090.ENSMUSP00000057984

Structurei

3D structure databases

ProteinModelPortaliQ9JJF3
SMRiQ9JJF3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 401JmjCPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ROX family. NO66 subfamily.Curated

Phylogenomic databases

eggNOGiKOG3706 Eukaryota
ENOG410YNEJ LUCA
GeneTreeiENSGT00390000000083
HOGENOMiHOG000030688
HOVERGENiHBG060021
InParanoidiQ9JJF3
KOiK16914
OMAiVYHLEEP
OrthoDBiEOG091G04K2
PhylomeDBiQ9JJF3
TreeFamiTF318659

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
IPR013109 NO66
PANTHERiPTHR13096:SF4 PTHR13096:SF4, 1 hit
PfamiView protein in Pfam
PF08007 Cupin_4, 1 hit
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JJF3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDELPNGNGA ALLKRGRGRR RRHPQSQPRG ASVLALPLRP RKIRRHRKSA
60 70 80 90 100
AASRVAALRA RALRSEDSDS KVAVASVRGK RKRPAELLEA SRSAEPRPVS
110 120 130 140 150
ARPRSASATL PSRVEGWAAL SRNLGTAAPP PPGSHADEPG RPRASPLQQV
160 170 180 190 200
LTELNGIPSS RRRAARLFEW LLAPLPPDHF YRRLWEREAV LVRRQDRSYY
210 220 230 240 250
EGLFSTADLD SMLRYEDVQF GQHLDAARYV DGRRETLNPP GRALPAAAWS
260 270 280 290 300
LYRAGCSLRL LCPQAFSPTV WQFLAVLQEQ FGSMAGSNVY LTPPDSQGFA
310 320 330 340 350
PHYDDIEAFV LQLEGRKLWR VYRPRDPSEE LALTSSPNFS QEDLGEPVLQ
360 370 380 390 400
TVLEPGDLLY FPRGFIHQAE CQDGVHSLHL TLSTYQRNTW GDFLEAVLPL
410 420 430 440 450
AVQAAIEENV EFRRGLPRDF MDYMGAQHSD SKDPRRTAFM EKVRVLVARL
460 470 480 490 500
GHFAPVDAVA DQRAKDFIHD SLPPVLTDRE RALSVHGLPV RWEAGEPVNV
510 520 530 540 550
GAQLTTETQV HMLQDGVARL VGEGGRLFLY HTVENSRVYH LEEPKCLEIH
560 570 580 590 600
PQQADAMELL LRSYPEFVRV GDLPCDSVED QLSLATMLYD KGLLLTKTPL

VPS
Length:603
Mass (Da):67,557
Last modified:January 19, 2010 - v2
Checksum:iDAB901939874D538
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti410V → E in BAA95038 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041553 mRNA Translation: BAA95038.1
CH466590 Genomic DNA Translation: EDL02754.1
BC138094 mRNA Translation: AAI38095.1
CCDSiCCDS26033.1
RefSeqiNP_076122.2, NM_023633.3
UniGeneiMm.251483

Genome annotation databases

EnsembliENSMUST00000053744; ENSMUSP00000057984; ENSMUSG00000046791
GeneIDi71952
KEGGimmu:71952
UCSCiuc007odz.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041553 mRNA Translation: BAA95038.1
CH466590 Genomic DNA Translation: EDL02754.1
BC138094 mRNA Translation: AAI38095.1
CCDSiCCDS26033.1
RefSeqiNP_076122.2, NM_023633.3
UniGeneiMm.251483

3D structure databases

ProteinModelPortaliQ9JJF3
SMRiQ9JJF3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215052, 2 interactors
IntActiQ9JJF3, 4 interactors
MINTiQ9JJF3
STRINGi10090.ENSMUSP00000057984

PTM databases

iPTMnetiQ9JJF3
PhosphoSitePlusiQ9JJF3

Proteomic databases

EPDiQ9JJF3
MaxQBiQ9JJF3
PaxDbiQ9JJF3
PeptideAtlasiQ9JJF3
PRIDEiQ9JJF3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053744; ENSMUSP00000057984; ENSMUSG00000046791
GeneIDi71952
KEGGimmu:71952
UCSCiuc007odz.2 mouse

Organism-specific databases

CTDi79697
MGIiMGI:1919202 Riox1

Phylogenomic databases

eggNOGiKOG3706 Eukaryota
ENOG410YNEJ LUCA
GeneTreeiENSGT00390000000083
HOGENOMiHOG000030688
HOVERGENiHBG060021
InParanoidiQ9JJF3
KOiK16914
OMAiVYHLEEP
OrthoDBiEOG091G04K2
PhylomeDBiQ9JJF3
TreeFamiTF318659

Miscellaneous databases

PROiPR:Q9JJF3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046791 Expressed in 245 organ(s), highest expression level in blastocyst
CleanExiMM_2410016O06RIK
GenevisibleiQ9JJF3 MM

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
IPR013109 NO66
PANTHERiPTHR13096:SF4 PTHR13096:SF4, 1 hit
PfamiView protein in Pfam
PF08007 Cupin_4, 1 hit
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIOX1_MOUSE
AccessioniPrimary (citable) accession number: Q9JJF3
Secondary accession number(s): B2RQV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: January 19, 2010
Last modified: September 12, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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