UniProtKB - Q9JJD0 (THA11_MOUSE)
Protein
THAP domain-containing protein 11
Gene
Thap11
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers.1 Publication
Miscellaneous
Was named 'Ronin' (a masterless Japanese samurai) by PubMed:18585351 because of its lack of apparent relationship to known 'master' regulator of pluripotency.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1 – 80 | THAP-typePROSITE-ProRule annotationAdd BLAST | 80 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- zinc ion binding Source: UniProtKB
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: THAP domain-containing protein 11Alternative name(s): Ronin |
Gene namesi | Name:Thap11 ORF Names:MNCb-2032 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1930964, Thap11 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mice show periimplantational lethality and have defects in the inner cell mass. Conditional knockout prevents the growth of ES cells while forced expression allows ES cells to proliferate without differentiation under conditions that normally do not promote self-renewal.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068654 | 1 – 305 | THAP domain-containing protein 11Add BLAST | 305 |
Proteomic databases
EPDi | Q9JJD0 |
MaxQBi | Q9JJD0 |
PaxDbi | Q9JJD0 |
PeptideAtlasi | Q9JJD0 |
PRIDEi | Q9JJD0 |
ProteomicsDBi | 263172 |
PTM databases
iPTMneti | Q9JJD0 |
PhosphoSitePlusi | Q9JJD0 |
Expressioni
Tissue specificityi
Mainly restricted to pluripotent cells of the developing embryo, to oocytes, and to certain regions of the adult brain.1 Publication
Developmental stagei
First appears at the 2-cell stage, intensifies during the 8-cell and compact morula stages, but subsides in the blastocyst.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000036442, Expressed in cleaving embryo and 288 other tissues |
Genevisiblei | Q9JJD0, MM |
Interactioni
Subunit structurei
Interacts (via coiled coil domain) with HCFC1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 208487, 1 interactor |
ELMi | Q9JJD0 |
STRINGi | 10090.ENSMUSP00000048994 |
Miscellaneous databases
RNActi | Q9JJD0, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 246 – 296 | Sequence analysisAdd BLAST | 51 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 234 – 237 | HCFC1-binding motif (HBM)By similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 95 – 100 | Poly-Ala | 6 | |
Compositional biasi | 104 – 137 | Gln-richAdd BLAST | 34 | |
Compositional biasi | 188 – 212 | Ala-richAdd BLAST | 25 |
Sequence similaritiesi
Belongs to the THAP11 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1 – 80 | THAP-typePROSITE-ProRule annotationAdd BLAST | 80 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QQ1Z, Eukaryota |
GeneTreei | ENSGT00390000006585 |
HOGENOMi | CLU_090879_1_0_1 |
InParanoidi | Q9JJD0 |
OMAi | DLRFYTF |
OrthoDBi | 1177412at2759 |
PhylomeDBi | Q9JJD0 |
TreeFami | TF331359 |
Family and domain databases
Gene3Di | 3.30.40.180, 1 hit |
InterProi | View protein in InterPro IPR006612, THAP_Znf IPR038441, THAP_Znf_sf |
Pfami | View protein in Pfam PF05485, THAP, 1 hit |
SMARTi | View protein in SMART SM00692, DM3, 1 hit SM00980, THAP, 1 hit |
PROSITEi | View protein in PROSITE PS50950, ZF_THAP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9JJD0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGFTCCVPG CYNNSHRDKA LHFYTFPKDA ELRRLWLKNV SRAGVSGCFS
60 70 80 90 100
TFQPTTGHRL CSVHFQGGRK TYTVRVPTIF PLRGVNERKV ARRPAGAAAA
110 120 130 140 150
RRRQQQQQQQ QQQQQQQQLQ QQQPSPSSST AQTTQLQPNL VSASAAVLLT
160 170 180 190 200
LQAAVDSNQA PGSVVPVSTT PSGDDVKPID LTVQVEFAAA EGAAAAAAAS
210 220 230 240 250
ELEAATAGLE AAECTLGPQL VVVGEEGFPD TGSDHSYSLS SGTTEEELLR
260 270 280 290 300
KLNEQRDILA LMEVKMKEMK GSIRHLRLTE AKLREELREK DRLLAMAVIR
KKHGM
Sequence cautioni
The sequence AAH03949 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB041579 mRNA Translation: BAA95063.1 AK165981 mRNA Translation: BAE38498.1 AK170457 mRNA Translation: BAE41810.1 CH466525 Genomic DNA Translation: EDL11318.1 BC003949 mRNA Translation: AAH03949.1 Different initiation. BC138966 mRNA Translation: AAI38967.1 BC138987 mRNA Translation: AAI38988.1 |
CCDSi | CCDS22616.1 |
RefSeqi | NP_067488.1, NM_021513.2 |
Genome annotation databases
Ensembli | ENSMUST00000040445; ENSMUSP00000048994; ENSMUSG00000036442 |
GeneIDi | 59016 |
KEGGi | mmu:59016 |
UCSCi | uc009nei.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB041579 mRNA Translation: BAA95063.1 AK165981 mRNA Translation: BAE38498.1 AK170457 mRNA Translation: BAE41810.1 CH466525 Genomic DNA Translation: EDL11318.1 BC003949 mRNA Translation: AAH03949.1 Different initiation. BC138966 mRNA Translation: AAI38967.1 BC138987 mRNA Translation: AAI38988.1 |
CCDSi | CCDS22616.1 |
RefSeqi | NP_067488.1, NM_021513.2 |
3D structure databases
BMRBi | Q9JJD0 |
SMRi | Q9JJD0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208487, 1 interactor |
ELMi | Q9JJD0 |
STRINGi | 10090.ENSMUSP00000048994 |
PTM databases
iPTMneti | Q9JJD0 |
PhosphoSitePlusi | Q9JJD0 |
Proteomic databases
EPDi | Q9JJD0 |
MaxQBi | Q9JJD0 |
PaxDbi | Q9JJD0 |
PeptideAtlasi | Q9JJD0 |
PRIDEi | Q9JJD0 |
ProteomicsDBi | 263172 |
Protocols and materials databases
Antibodypediai | 29649, 272 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000040445; ENSMUSP00000048994; ENSMUSG00000036442 |
GeneIDi | 59016 |
KEGGi | mmu:59016 |
UCSCi | uc009nei.2, mouse |
Organism-specific databases
CTDi | 57215 |
MGIi | MGI:1930964, Thap11 |
Phylogenomic databases
eggNOGi | ENOG502QQ1Z, Eukaryota |
GeneTreei | ENSGT00390000006585 |
HOGENOMi | CLU_090879_1_0_1 |
InParanoidi | Q9JJD0 |
OMAi | DLRFYTF |
OrthoDBi | 1177412at2759 |
PhylomeDBi | Q9JJD0 |
TreeFami | TF331359 |
Miscellaneous databases
BioGRID-ORCSi | 59016, 25 hits in 54 CRISPR screens |
ChiTaRSi | Thap11, mouse |
PROi | PR:Q9JJD0 |
RNActi | Q9JJD0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036442, Expressed in cleaving embryo and 288 other tissues |
Genevisiblei | Q9JJD0, MM |
Family and domain databases
Gene3Di | 3.30.40.180, 1 hit |
InterProi | View protein in InterPro IPR006612, THAP_Znf IPR038441, THAP_Znf_sf |
Pfami | View protein in Pfam PF05485, THAP, 1 hit |
SMARTi | View protein in SMART SM00692, DM3, 1 hit SM00980, THAP, 1 hit |
PROSITEi | View protein in PROSITE PS50950, ZF_THAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THA11_MOUSE | |
Accessioni | Q9JJD0Primary (citable) accession number: Q9JJD0 Secondary accession number(s): Q3TCZ5, Q99KZ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 11, 2003 |
Last sequence update: | October 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families