UniProtKB - Q9JJ48 (ZC3H8_MOUSE)
Zinc finger CCCH domain-containing protein 8
Zc3h8
Functioni
Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Acts as a transcriptional repressor of the GATA3 promoter. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis (By similarity).
Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene.
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 205 – 232 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 234 – 261 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 262 – 285 | C3H1-type 3PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
- RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- negative regulation of T cell differentiation in thymus Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of thymocyte apoptotic process Source: UniProtKB
- positive regulation of transcription by RNA polymerase III Source: UniProtKB
- response to antibiotic Source: UniProtKB
- snRNA transcription by RNA polymerase II Source: UniProtKB
- snRNA transcription by RNA polymerase III Source: UniProtKB
- T cell homeostasis Source: UniProtKB
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | Apoptosis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-6807505, RNA polymerase II transcribes snRNA genes |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger CCCH domain-containing protein 8Alternative name(s): Fetal liver zinc finger protein 1 |
Gene namesi | Name:Zc3h8 Synonyms:Fliz1, Zc3hdc8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1930128, Zc3h8 |
VEuPathDBi | HostDB:ENSMUSG00000027387 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Colocalizes with coilin in subnuclear cajal and histone locus bodies. Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes (By similarity).By similarity
Nucleus
- Cajal body Source: UniProtKB
- histone locus body Source: UniProtKB
- nuclear body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- transcription elongation factor complex Source: UniProtKB
Other locations
- transcriptionally active chromatin Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213904 | 1 – 305 | Zinc finger CCCH domain-containing protein 8Add BLAST | 305 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 33 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 44 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 67 | PhosphoserineBy similarity | 1 | |
Modified residuei | 85 | PhosphoserineCombined sources | 1 | |
Modified residuei | 91 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9JJ48 |
MaxQBi | Q9JJ48 |
PaxDbi | Q9JJ48 |
PRIDEi | Q9JJ48 |
ProteomicsDBi | 275269 |
PTM databases
iPTMneti | Q9JJ48 |
PhosphoSitePlusi | Q9JJ48 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000027387, Expressed in zygote and 236 other tissues |
Genevisiblei | Q9JJ48, MM |
Interactioni
Subunit structurei
Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2.
Interacts with ICE1 (via C-terminus domain) (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000028866 |
Miscellaneous databases
RNActi | Q9JJ48, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 191 | DisorderedSequence analysisAdd BLAST | 191 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 36 – 58 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 85 – 101 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 141 – 157 | Basic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 176 – 190 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 205 – 232 | C3H1-type 1PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 234 – 261 | C3H1-type 2PROSITE-ProRule annotationAdd BLAST | 28 | |
Zinc fingeri | 262 – 285 | C3H1-type 3PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1040, Eukaryota |
GeneTreei | ENSGT00940000161918 |
HOGENOMi | CLU_059008_0_0_1 |
InParanoidi | Q9JJ48 |
OMAi | QKYIRAK |
OrthoDBi | 145192at2759 |
PhylomeDBi | Q9JJ48 |
TreeFami | TF318143 |
Family and domain databases
InterProi | View protein in InterPro IPR045124, Su(sable)-like IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf |
PANTHERi | PTHR13119, PTHR13119, 1 hit |
Pfami | View protein in Pfam PF00642, zf-CCCH, 1 hit |
SMARTi | View protein in SMART SM00356, ZnF_C3H1, 3 hits |
SUPFAMi | SSF90229, SSF90229, 3 hits |
PROSITEi | View protein in PROSITE PS50103, ZF_C3H1, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDFENLFSKP PNPALGKKPA ADPEERIDEI DGTEVEETQT EKVKWKVKRD
60 70 80 90 100
REQIPKKFKH LGNAATSPKS LLRKKSRSKD YDPYSDGETC SQGSEDNFDK
110 120 130 140 150
ELQQYIQAKE MANAAQPSLL PEESVKKAGA EGTQQTAKQK NKKSKAGHKK
160 170 180 190 200
VKQKKMKRKW PGTGDKGSRA LLKNSGSREQ TDEPEEKQPR VRMSQGFINQ
210 220 230 240 250
HTVERKGKQV CKYFLERKCI KGDQCKFDHD AEIEKKKEMC KYYVQGYCTK
260 270 280 290 300
GENCLYLHSE YPCKFYHTGT KCYQGDHCNF SHAPLTAETQ ELLAKVLDTD
KKSCK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 124 | S → P in AAF74513 (PubMed:11318609).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061961 mRNA Translation: AAF74513.1 BC048687 mRNA Translation: AAH48687.1 |
CCDSi | CCDS16718.1 |
RefSeqi | NP_065619.2, NM_020594.2 |
Genome annotation databases
Ensembli | ENSMUST00000028866; ENSMUSP00000028866; ENSMUSG00000027387 |
GeneIDi | 57432 |
KEGGi | mmu:57432 |
UCSCi | uc033hqb.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061961 mRNA Translation: AAF74513.1 BC048687 mRNA Translation: AAH48687.1 |
CCDSi | CCDS16718.1 |
RefSeqi | NP_065619.2, NM_020594.2 |
3D structure databases
SMRi | Q9JJ48 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000028866 |
PTM databases
iPTMneti | Q9JJ48 |
PhosphoSitePlusi | Q9JJ48 |
Proteomic databases
EPDi | Q9JJ48 |
MaxQBi | Q9JJ48 |
PaxDbi | Q9JJ48 |
PRIDEi | Q9JJ48 |
ProteomicsDBi | 275269 |
Protocols and materials databases
Antibodypediai | 47908, 238 antibodies from 30 providers |
DNASUi | 57432 |
Genome annotation databases
Ensembli | ENSMUST00000028866; ENSMUSP00000028866; ENSMUSG00000027387 |
GeneIDi | 57432 |
KEGGi | mmu:57432 |
UCSCi | uc033hqb.1, mouse |
Organism-specific databases
CTDi | 84524 |
MGIi | MGI:1930128, Zc3h8 |
VEuPathDBi | HostDB:ENSMUSG00000027387 |
Phylogenomic databases
eggNOGi | KOG1040, Eukaryota |
GeneTreei | ENSGT00940000161918 |
HOGENOMi | CLU_059008_0_0_1 |
InParanoidi | Q9JJ48 |
OMAi | QKYIRAK |
OrthoDBi | 145192at2759 |
PhylomeDBi | Q9JJ48 |
TreeFami | TF318143 |
Enzyme and pathway databases
Reactomei | R-MMU-6807505, RNA polymerase II transcribes snRNA genes |
Miscellaneous databases
BioGRID-ORCSi | 57432, 14 hits in 63 CRISPR screens |
PROi | PR:Q9JJ48 |
RNActi | Q9JJ48, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027387, Expressed in zygote and 236 other tissues |
Genevisiblei | Q9JJ48, MM |
Family and domain databases
InterProi | View protein in InterPro IPR045124, Su(sable)-like IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf |
PANTHERi | PTHR13119, PTHR13119, 1 hit |
Pfami | View protein in Pfam PF00642, zf-CCCH, 1 hit |
SMARTi | View protein in SMART SM00356, ZnF_C3H1, 3 hits |
SUPFAMi | SSF90229, SSF90229, 3 hits |
PROSITEi | View protein in PROSITE PS50103, ZF_C3H1, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZC3H8_MOUSE | |
Accessioni | Q9JJ48Primary (citable) accession number: Q9JJ48 Secondary accession number(s): Q80X92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot