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Protein

3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase

Gene

Ebp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of Delta8-sterols to their corresponding Delta7-isomers.By similarity

Miscellaneous

Binds to the phenylalkylamine calcium-ion antagonist emopamil, an anti-ischemic drug.

Catalytic activityi

5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol.By similarity

Pathwayi: cholesterol biosynthesis

This protein is involved in the pathway cholesterol biosynthesis, which is part of Steroid biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway cholesterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • cholesterol biosynthetic process Source: RGD
  • hemopoiesis Source: Ensembl
  • sterol biosynthetic process Source: RGD

Keywordsi

Molecular functionIsomerase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi5.3.3.5 5301
ReactomeiR-RNO-6807047 Cholesterol biosynthesis via desmosterol
R-RNO-6807062 Cholesterol biosynthesis via lathosterol
SABIO-RKiQ9JJ46
UniPathwayi
UPA00063

Names & Taxonomyi

Protein namesi
Recommended name:
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EC:5.3.3.5)
Alternative name(s):
Cholestenol Delta-isomerase
Delta(8)-Delta(7) sterol isomerase
Short name:
D8-D7 sterol isomerase
Emopamil-binding protein
Sterol 8-isomerase
Gene namesi
Name:Ebp
Synonyms:Rsi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi620957 Ebp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001743442 – 2303-beta-hydroxysteroid-Delta(8),Delta(7)-isomeraseAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9JJ46
PRIDEiQ9JJ46

Expressioni

Gene expression databases

BgeeiENSRNOG00000004903 Expressed in 10 organ(s), highest expression level in liver
GenevisibleiQ9JJ46 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007015

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 204EXPERAPROSITE-ProRule annotationAdd BLAST144

Sequence similaritiesi

Belongs to the EBP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4826 Eukaryota
ENOG41101ES LUCA
GeneTreeiENSGT00530000063715
HOGENOMiHOG000204543
HOVERGENiHBG018176
InParanoidiQ9JJ46
KOiK01824
OMAiSPLHPYW
OrthoDBiEOG091G0KCI
PhylomeDBiQ9JJ46
TreeFamiTF314716

Family and domain databases

InterProiView protein in InterPro
IPR007905 EBP
IPR033118 EXPERA
PANTHERiPTHR14207 PTHR14207, 1 hit
PfamiView protein in Pfam
PF05241 EBP, 1 hit
PROSITEiView protein in PROSITE
PS51751 EXPERA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JJ46-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTNMLPLHP YWPRHLRLDN FVPNDLPTWH ILVGLFSFSG VLIVITWLLS
60 70 80 90 100
SRVSVVPLGT GRRLALCWFA VCTFIHLVIE GWFSFYHEIL LEDQAFLSQL
110 120 130 140 150
WKEYSKGDSR YILSDGFIVC MESVTACLWG PLSLWVVIAF LRHQPFRFVL
160 170 180 190 200
QLVVSVGQIY GDVLYFLTEL RDGFQHGELG HPLYFWFYFV IMNAIWLVIP
210 220 230
GILVFDAIKH LTNAQSMLDN KVMKIKSKHN
Length:230
Mass (Da):26,738
Last modified:January 23, 2007 - v3
Checksum:i7CBBF252A0B9E546
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071501 mRNA Translation: AAF74807.1
AF318617 Genomic DNA Translation: AAQ14592.1
RefSeqiNP_476478.1, NM_057137.1
XP_006256761.1, XM_006256699.3
UniGeneiRn.19436

Genome annotation databases

EnsembliENSRNOT00000007015; ENSRNOP00000007015; ENSRNOG00000004903
GeneIDi117278
KEGGirno:117278
UCSCiRGD:620957 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071501 mRNA Translation: AAF74807.1
AF318617 Genomic DNA Translation: AAQ14592.1
RefSeqiNP_476478.1, NM_057137.1
XP_006256761.1, XM_006256699.3
UniGeneiRn.19436

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007015

Proteomic databases

PaxDbiQ9JJ46
PRIDEiQ9JJ46

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007015; ENSRNOP00000007015; ENSRNOG00000004903
GeneIDi117278
KEGGirno:117278
UCSCiRGD:620957 rat

Organism-specific databases

CTDi10682
RGDi620957 Ebp

Phylogenomic databases

eggNOGiKOG4826 Eukaryota
ENOG41101ES LUCA
GeneTreeiENSGT00530000063715
HOGENOMiHOG000204543
HOVERGENiHBG018176
InParanoidiQ9JJ46
KOiK01824
OMAiSPLHPYW
OrthoDBiEOG091G0KCI
PhylomeDBiQ9JJ46
TreeFamiTF314716

Enzyme and pathway databases

UniPathwayi
UPA00063

BRENDAi5.3.3.5 5301
ReactomeiR-RNO-6807047 Cholesterol biosynthesis via desmosterol
R-RNO-6807062 Cholesterol biosynthesis via lathosterol
SABIO-RKiQ9JJ46

Miscellaneous databases

PROiPR:Q9JJ46

Gene expression databases

BgeeiENSRNOG00000004903 Expressed in 10 organ(s), highest expression level in liver
GenevisibleiQ9JJ46 RN

Family and domain databases

InterProiView protein in InterPro
IPR007905 EBP
IPR033118 EXPERA
PANTHERiPTHR14207 PTHR14207, 1 hit
PfamiView protein in Pfam
PF05241 EBP, 1 hit
PROSITEiView protein in PROSITE
PS51751 EXPERA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEBP_RAT
AccessioniPrimary (citable) accession number: Q9JJ46
Secondary accession number(s): Q548M8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 114 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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