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Protein

Pyrin

Gene

Mefv

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it may also have a positive effect in the inflammatory pathway. In different experimental systems, it has been shown to activate IL1B production. It has also been shown to be required for PSTPIP1-induced PYCARD oligomerization and for formation of inflammasomes. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.By similarity

Miscellaneous

Degraded along with the delivery of its substrates to autolysosomal compartments (at protein level).By similarity

Caution

Lacks the B30.2/SPRY domain found in the human ortholog, thus may have divergent function(s).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri439 – 481B box-typePROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • identical protein binding Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processImmunity, Inflammatory response, Innate immunity
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-844456 The NLRP3 inflammasome

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrin
Alternative name(s):
Marenostrin
Gene namesi
Name:Mefv
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1859396 Mefv

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Disruption phenotypei

Animals are grossly normal, with no obvious changes in thymus, spleen or lymph nodes. In vitro, resident peritoneal macrophage cells show enhanced IL1B and IL18 release in response to inflammatory stimuli.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002203651 – 767PyrinAdd BLAST767

Proteomic databases

MaxQBiQ9JJ26
PRIDEiQ9JJ26

PTM databases

iPTMnetiQ9JJ26
PhosphoSitePlusiQ9JJ26

Expressioni

Tissue specificityi

Expressed in spleen peripheral blood granulocytes. Not expressed in lymphocytes, thymus, testis, ovary, heart, brain, lung, liver, kidney and muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000022534
CleanExiMM_MEFV
ExpressionAtlasiQ9JJ26 baseline and differential
GenevisibleiQ9JJ26 MM

Interactioni

Subunit structurei

Homotrimer. Interacts (via the B box-type zinc finger) with PSTPIP1. Interacts (via the B30.2/SPRY domain) with several components of the inflammasome complex, including CASP1 p20 and p10 subunits, CASP5, PYCARD, NLRP1, NLRP2 AND NLRP3, as well as with unprocessed IL1B; this interaction may lead to autophagic degradation of these proteins. Interacts with NFKBIA and RELA. Interacts weakly with VASP and ACTR3. Interacts with active ULK1 (phosphorylated on 'Ser-317') and BECN1 simultaneously. Also interacts with ATG16L1 (via WD repeats), and with ATG8 family members, including GABARAP, GABARAPL1 and, to a lesser extent, GABARAPL2, MAP1LC3A/LC3A and MAP1LC3C/LC3C. Interacts with TRIM21.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ9JJ26
SMRiQ9JJ26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 92PyrinPROSITE-ProRule annotationCuratedAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni489 – 647Required for homotrimerization and induction of pyroptosomesBy similarityAdd BLAST159

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili481 – 510Sequence analysisAdd BLAST30

Domaini

The B box-type zinc finger interacts, possibly intramolecularly, with the pyrin domain; this may be an autoinhibitory mechanism released by PSTPIP1 binding.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri439 – 481B box-typePROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000118838
HOGENOMiHOG000113552
HOVERGENiHBG006343
InParanoidiQ9JJ26
KOiK12803

Family and domain databases

CDDicd00021 BBOX, 1 hit
InterProiView protein in InterPro
IPR004020 DAPIN
IPR011029 DEATH-like_dom_sf
IPR028841 Pyrin
IPR000315 Znf_B-box
PANTHERiPTHR45323 PTHR45323, 2 hits
PfamiView protein in Pfam
PF02758 PYRIN, 1 hit
PF00643 zf-B_box, 1 hit
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM01289 PYRIN, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
PROSITEiView protein in PROSITE
PS50824 DAPIN, 1 hit
PS50119 ZF_BBOX, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JJ26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTLGDHLL NTLEELLPYD FEKFKFKLQN TSLEKGHSKI PRGHMQMARP
60 70 80 90 100
VKLASLLITY YGEEYAVRLT LQILRATNQR QLAEELRKAT GTEHLIEENR
110 120 130 140 150
VGGSVQSSVE NKAKSVKVPD VPEGDGTQQN NDESDTLPSS QAEVGKGPQK
160 170 180 190 200
KSLTKRKDQR GPESLDSQTK PWTRSTAPLY RRTQGTQSPG DKESTASAQL
210 220 230 240 250
RRNVSSAGRL QGLYNNAPGR RESKKAEVYV YLPSGKKRPR SLEITTYSRE
260 270 280 290 300
GEPPNSEVLP TQEETRNGSL IRMRTATLNG RTTGALEKGT GIPEHSMVLD
310 320 330 340 350
EKTFRNMSSK TSLIGEERCP TSWTENGNGS PETTESSGET AGSILSDPEV
360 370 380 390 400
PLSLCEKPAK TPEDPASLGQ AACEGRSQDK AVCPLCHTQE GDLRGDTCVQ
410 420 430 440 450
SSCSCSIAPG DPKASGRCSI CFQCQGLLAR KSCEAQSPQS LPQCPRHMKQ
460 470 480 490 500
VLLLFCEDHR EPICLICRLS LEHQGHRVRP IEEAALEYKE QIREQLERLR
510 520 530 540 550
EMRGYVEEHR LQGDKKTDDF LKQTEIQKQK ISCPLEKLYQ LLEKQEQLFV
560 570 580 590 600
TWLQELSQTI SKVRETYYTR VSLLDEMIEE LEAKQDQPEW DLMQDIGITL
610 620 630 640 650
HRAKMMSASE LLDTPPGVKE KLHLLYQKSK SVEKNMQCFS EMLSSEMAFS
660 670 680 690 700
ASDVAKWEGR QPSATQVQGL VPTVHLKCDG AHTQDCDVVF YPEREAGGSE
710 720 730 740 750
PKDYLHPQPA QDTPELHEIH SRNNKRKFKS FLKWKPSFSR TDWRLRTCCY
760
RDLDQAAAHP NLIFSMI
Length:767
Mass (Da):86,409
Last modified:July 27, 2011 - v2
Checksum:i828E59E4E8DF5549
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti543E → G in AAF03766 (PubMed:10818206).Curated1
Sequence conflicti565E → G in AAF03766 (PubMed:10818206).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143409 mRNA Translation: AAF03766.1
AC139347 Genomic DNA No translation available.
CCDSiCCDS27907.1
RefSeqiNP_062326.2, NM_019453.2
UniGeneiMm.143718

Genome annotation databases

EnsembliENSMUST00000023180; ENSMUSP00000023180; ENSMUSG00000022534
GeneIDi54483
KEGGimmu:54483
UCSCiuc007xym.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMEFV_MOUSE
AccessioniPrimary (citable) accession number: Q9JJ26
Secondary accession number(s): E9QN30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: July 27, 2011
Last modified: November 22, 2017
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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