Na(+)/H(+) exchange regulatory cofactor NHE-RF1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• beta-2 adrenergic receptor binding Source: RGD
• beta-catenin binding Source: RGD
• chloride channel regulator activity Source: UniProtKB
• dopamine receptor binding Source: RGD
• growth factor receptor binding Source: RGD
• molecular adaptor activity Source: RGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Na+-H+ exchanger regulatory factor 1 (NHERF1) PDZ scaffold binds an internal binding site in the scavenger receptor megalin."
    Slattery C., Jenkin K.A., Lee A., Simcocks A.C., McAinch A.J., Poronnik P., Hryciw D.H.
    Cell Physiol Biochem 27:171-178(2011) [PubMed] [Europe PMC] [Abstract]
• myosin II binding Source: RGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "EBP50 is involved in the regulation of vascular smooth muscle cell migration and cytokinesis."
    Baeyens N., de Meester C., Yerna X., Morel N.
    J Cell Biochem 112:2574-2584(2011) [PubMed] [Europe PMC] [Abstract]
• PDZ domain binding Source: RGD <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "Na+-H+ exchanger regulatory factor 1 (NHERF1) PDZ scaffold binds an internal binding site in the scavenger receptor megalin."
    Slattery C., Jenkin K.A., Lee A., Simcocks A.C., McAinch A.J., Poronnik P., Hryciw D.H.
    Cell Physiol Biochem 27:171-178(2011) [PubMed] [Europe PMC] [Abstract]
• phosphatase binding Source: RGD
• protein-containing complex binding Source: RGDInferred from physical interactionⁱ
  • "Analysis of different complexes of type IIa sodium-dependent phosphate transporter in rat renal cortex using blue-native polyacrylamide gel electrophoresis."
    Tanimura A., Yamada F., Saito A., Ito M., Kimura T., Anzai N., Horie D., Yamamoto H., Miyamoto K., Taketani Y., Takeda E.
    J Med Invest 58:140-147(2011) [PubMed] [Europe PMC] [Abstract]
• protein domain specific binding Source: RGDInferred from physical interactionⁱ
  • "NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli."
    Giral H., Cranston D., Lanzano L., Caldas Y., Sutherland E., Rachelson J., Dobrinskikh E., Weinman E.J., Doctor R.B., Gratton E., Levi M.
    J Biol Chem 287:35047-35056(2012) [PubMed] [Europe PMC] [Abstract]
• protein-membrane adaptor activity Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• protein N-terminus binding Source: RGDInferred from physical interactionⁱ
  • "Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1."
    Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E.
    J Biol Chem 281:1796-1807(2006) [PubMed] [Europe PMC] [Abstract]
• protein self-association Source: RGD
• signaling receptor binding Source: RGD
• type 2 metabotropic glutamate receptor binding Source: RGD
• type 3 metabotropic glutamate receptor binding Source: RGD

GO - Biological processⁱ

• actin cytoskeleton organization Source: RGD
• adenylate cyclase-activating dopamine receptor signaling pathway Source: RGD
• auditory receptor cell stereocilium organization Source: RGD
• bile acid secretion Source: UniProtKB
• cAMP-mediated signaling Source: RGD
• cellular phosphate ion homeostasis Source: RGD
• cellular protein localization Source: RGD
• cerebrospinal fluid circulation Source: RGD
• cilium organization Source: RGD
• establishment of epithelial cell apical/basal polarity Source: RGD
• establishment of Golgi localization Source: RGD
• gamma-aminobutyric acid import Source: ARUK-UCL <p>Inferred from Genetic Interaction</p> <p>Used to describe "traditional" genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "Assembly of Taurine Transporter (Slc6a6) with Na⁺-H⁺ Exchanger Regulatory Factor 1 (Slc9a3r1) Improves GABA Transport Activity by Increasing the Maximum Transport Velocity."
    Tachikawa M., Yokoyama R., Akanuma S.I., Hosoya K.I.
    Biol Pharm Bull 41:338-341(2018) [PubMed] [Europe PMC] [Abstract]
• gland morphogenesis Source: RGD
• glutathione transport Source: UniProtKB
• import across plasma membrane Source: ARUK-UCLInferred from genetic interactionⁱ
  • "Assembly of Taurine Transporter (Slc6a6) with Na⁺-H⁺ Exchanger Regulatory Factor 1 (Slc9a3r1) Improves GABA Transport Activity by Increasing the Maximum Transport Velocity."
    Tachikawa M., Yokoyama R., Akanuma S.I., Hosoya K.I.
    Biol Pharm Bull 41:338-341(2018) [PubMed] [Europe PMC] [Abstract]
• maintenance of epithelial cell apical/basal polarity Source: RGD
• microvillus assembly Source: RGD
• negative regulation of canonical Wnt signaling pathway Source: RGD
• negative regulation of cell migration Source: RGD
• negative regulation of cell motility Source: UniProtKB
• negative regulation of cell population proliferation Source: RGD
• negative regulation of ERK1 and ERK2 cascade Source: RGD
• negative regulation of mitotic cell cycle Source: RGD
• negative regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
• negative regulation of platelet-derived growth factor receptor signaling pathway Source: UniProtKB
• negative regulation of protein kinase B signaling Source: UniProtKB
• negative regulation of sodium:proton antiporter activity Source: RGD
• negative regulation of sodium ion transport Source: RGD
• nuclear migration Source: RGD
• phospholipase C-activating dopamine receptor signaling pathway Source: RGD
• plasma membrane organization Source: RGD
• positive regulation of intrinsic apoptotic signaling pathway Source: RGD
• positive regulation of ion transmembrane transport Source: RGDInferred from direct assayⁱ
  • "Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1."
    Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E.
    J Biol Chem 281:1796-1807(2006) [PubMed] [Europe PMC] [Abstract]
• protein localization to plasma membrane Source: RGD
• regulation of cell shape Source: RGD
• regulation of cell size Source: RGD
• regulation of excretion Source: RGD
• regulation of protein kinase activity Source: UniProtKB
• renal absorption Source: UniProtKB
• renal phosphate ion absorption Source: UniProtKB
• renal sodium ion transport Source: RGD
• sensory perception of sound Source: RGD
• Wnt signaling pathway Source: UniProtKB-KW

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• beta-2 adrenergic receptor binding Source: RGD
• beta-catenin binding Source: RGD
• dopamine receptor binding Source: RGD
• growth factor receptor binding Source: RGD
• myosin II binding Source: RGDInferred from direct assayⁱ
  • "EBP50 is involved in the regulation of vascular smooth muscle cell migration and cytokinesis."
    Baeyens N., de Meester C., Yerna X., Morel N.
    J Cell Biochem 112:2574-2584(2011) [PubMed] [Europe PMC] [Abstract]
• PDZ domain binding Source: RGDInferred from physical interactionⁱ
  • "Na+-H+ exchanger regulatory factor 1 (NHERF1) PDZ scaffold binds an internal binding site in the scavenger receptor megalin."
    Slattery C., Jenkin K.A., Lee A., Simcocks A.C., McAinch A.J., Poronnik P., Hryciw D.H.
    Cell Physiol Biochem 27:171-178(2011) [PubMed] [Europe PMC] [Abstract]
• phosphatase binding Source: RGD
• protein domain specific binding Source: RGDInferred from physical interactionⁱ
  • "NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli."
    Giral H., Cranston D., Lanzano L., Caldas Y., Sutherland E., Rachelson J., Dobrinskikh E., Weinman E.J., Doctor R.B., Gratton E., Levi M.
    J Biol Chem 287:35047-35056(2012) [PubMed] [Europe PMC] [Abstract]
• protein N-terminus binding Source: RGDInferred from physical interactionⁱ
  • "Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1."
    Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E.
    J Biol Chem 281:1796-1807(2006) [PubMed] [Europe PMC] [Abstract]
• protein self-association Source: RGD
• signaling receptor binding Source: RGD
• type 2 metabotropic glutamate receptor binding Source: RGD
• type 3 metabotropic glutamate receptor binding Source: RGD

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MSADAAAGEP LPRLCCLEKG PNGYGFHLHG EKGKVGQFIR LVEPGSPAEK 
        60         70         80         90        100
SGLLAGDRLV EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLK 
       110        120        130        140        150
KLGVPIREEL LRAQEKSEHT EPPAAADTKK AGDQNEAEKS HLERCELRPR 
       160        170        180        190        200
LCTMKKGPNG YGFNLHSDKS KPGQFIRAVD PDSPAEASGL RAQDRIVEVN 
       210        220        230        240        250
GVCMEGKQHG DVVSAIKAGG DEAKLLVVDK ETDEFFKKCR VTPSQEHLDG 
       260        270        280        290        300
PLPEPFSNGE IQKENSREAL VEPASESPRP ALARSASSDT SEELNAQDSP 
       310        320        330        340        350
KRHDSTEPSS TSSSSDPILD FNISLAVAKE RAHQKRSSKR APQMDWSKKN 

ELFSNL                                                 

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ