Slc9a3r1

Functionⁱ

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules (By similarity).By similarity

GO - Molecular functionⁱ

beta-2 adrenergic receptor binding Source: Ensembl
beta-catenin binding Source: Ensembl
chloride channel regulator activity Source: UniProtKB
dopamine receptor binding Source: Ensembl
growth factor receptor binding Source: Ensembl
myosin II binding
Source: RGD
Inferred from direct assayⁱ
- 21598299
PDZ domain binding
Source: RGD
Inferred from physical interactionⁱ
- 21325834
phosphatase binding Source: Ensembl
protein-containing complex binding
Source: RGD
Inferred from physical interactionⁱ
- 21372499
protein-containing complex scaffold activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 21325834
protein domain specific binding
Source: RGD
Inferred from physical interactionⁱ
- 22904329
protein N-terminus binding
Source: RGD
Inferred from physical interactionⁱ
- 16234233
protein self-association Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin cytoskeleton organization Source: Ensembl
adenylate cyclase-activating dopamine receptor signaling pathway Source: Ensembl
auditory receptor cell stereocilium organization Source: Ensembl
bile acid secretion Source: UniProtKB
cellular phosphate ion homeostasis Source: Ensembl
establishment of epithelial cell apical/basal polarity Source: Ensembl
establishment of Golgi localization Source: Ensembl
gland morphogenesis Source: Ensembl
glutathione transport Source: UniProtKB
microvillus assembly Source: Ensembl
negative regulation of canonical Wnt signaling pathway Source: Ensembl
negative regulation of cell migration Source: Ensembl
negative regulation of cell motility Source: UniProtKB
negative regulation of cell proliferation Source: Ensembl
negative regulation of ERK1 and ERK2 cascade Source: Ensembl
negative regulation of mitotic cell cycle Source: Ensembl
negative regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
negative regulation of platelet-derived growth factor receptor signaling pathway Source: UniProtKB
negative regulation of protein kinase B signaling Source: UniProtKB
negative regulation of sodium:proton antiporter activity Source: Ensembl
nuclear migration Source: Ensembl
phospholipase C-activating dopamine receptor signaling pathway Source: Ensembl
positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
positive regulation of ion transmembrane transport
Source: RGD
Inferred from direct assayⁱ
- 16234233
protein localization to plasma membrane Source: Ensembl
regulation of cell shape Source: Ensembl
regulation of cell size Source: Ensembl
regulation of excretion Source: Ensembl
regulation of protein kinase activity Source: UniProtKB
renal absorption Source: UniProtKB
renal phosphate ion absorption Source: UniProtKB
renal sodium ion transport Source: Ensembl
sensory perception of sound Source: Ensembl
Wnt signaling pathway Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Wnt signaling pathway

Biological process

Wnt signaling pathway

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Short name: NHERF-1 Alternative name(s): Ezrin-radixin-moesin-binding phosphoprotein 50 Short name: EBP50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1
Gene namesⁱ	Name:Slc9a3r1 Synonyms:Nherf, Nherf1
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 10

Organism-specific databases

Rat genome database More...RGDⁱ	708538 Slc9a3r1

RGDⁱ

708538 Slc9a3r1

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000096802	2 – 356	Na(+)/H(+) exchange regulatory cofactor NHE-RF1Add BLAST		355

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineBy similarity	1
Modified residueⁱ	2	PhosphoserineCombined sources	1
Modified residueⁱ	46	PhosphoserineBy similarity	1
Modified residueⁱ	266	PhosphoserineBy similarity	1
Modified residueⁱ	277	PhosphoserineCombined sources	1
Modified residueⁱ	287	PhosphoserineCombined sources	1
Modified residueⁱ	288	PhosphoserineCombined sources	1
Modified residueⁱ	290	PhosphothreonineCombined sources	1
Modified residueⁱ	291	PhosphoserineCombined sources	1
Modified residueⁱ	299	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	Q9JJ19
PRIDEⁱ	Q9JJ19

PTM databases

iPTMnetⁱ	Q9JJ19
PhosphoSitePlusⁱ	Q9JJ19
SwissPalmⁱ	Q9JJ19

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000003232 Expressed in 9 organ(s), highest expression level in adult mammalian kidney
Genevisibleⁱ	Q9JJ19 RN

Interactionⁱ

Subunit structureⁱ

Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2, CFTR and PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Binds ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1, PLCB3, PDZK1 and CLCN3. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1 (By similarity). Interacts with SLC4A7. Directly interacts with HTR4 (By similarity). Interacts with TRPC4 (via the PDZ-binding domain) (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells. Interacts with SLC34A1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity). Interacts with SLC26A3 (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Asic3	O35240	5	EBI-982391,EBI-982374

With

Entry

#Exp.

IntAct

Notes

Asic3

O35240

5

EBI-982391,EBI-982374

GO - Molecular functionⁱ

beta-2 adrenergic receptor binding Source: Ensembl
beta-catenin binding Source: Ensembl
dopamine receptor binding Source: Ensembl
growth factor receptor binding Source: Ensembl
myosin II binding
Source: RGD
Inferred from direct assayⁱ
- 21598299
PDZ domain binding
Source: RGD
Inferred from physical interactionⁱ
- 21325834
phosphatase binding Source: Ensembl
protein-containing complex scaffold activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 21325834
protein domain specific binding
Source: RGD
Inferred from physical interactionⁱ
- 22904329
protein N-terminus binding
Source: RGD
Inferred from physical interactionⁱ
- 16234233
protein self-association Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	248735, 3 interactors
IntActⁱ	Q9JJ19, 1 interactor
STRINGⁱ	10116.ENSRNOP00000004351

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9JJ19
SMRⁱ	Q9JJ19
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	14 – 94	PDZ 1PROSITE-ProRule annotationAdd BLAST		81
Domainⁱ	151 – 231	PDZ 2PROSITE-ProRule annotationAdd BLAST		81

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	ENOG410ITAB Eukaryota ENOG4110SZZ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000062999
HOGENOMⁱ	HOG000089940
HOVERGENⁱ	HBG052616
InParanoidⁱ	Q9JJ19
KEGG Orthology (KO) More...KOⁱ	K13365
OMAⁱ	LCAMKKG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G086H
PhylomeDBⁱ	Q9JJ19
TreeFamⁱ	TF350449

Family and domain databases

InterProⁱ	View protein in InterPro IPR015098 EBP50_C-term IPR031199 NHERF-1 IPR017300 NHERF-1/NHERF-2 IPR001478 PDZ IPR036034 PDZ_sf
PANTHERⁱ	PTHR14191:SF7 PTHR14191:SF7, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09007 EBP50_C, 1 hit PF00595 PDZ, 2 hits
PIRSFⁱ	PIRSF037866 EBP50, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD283022 EBP50_C-term, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 2 hits
SUPFAMⁱ	SSF50156 SSF50156, 2 hits
PROSITEⁱ	View protein in PROSITE PS50106 PDZ, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9JJ19-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSADAAAGEP LPRLCCLEKG PNGYGFHLHG EKGKVGQFIR LVEPGSPAEK 
        60         70         80         90        100
SGLLAGDRLV EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLK 
       110        120        130        140        150
KLGVPIREEL LRAQEKSEHT EPPAAADTKK AGDQNEAEKS HLERCELRPR 
       160        170        180        190        200
LCTMKKGPNG YGFNLHSDKS KPGQFIRAVD PDSPAEASGL RAQDRIVEVN 
       210        220        230        240        250
GVCMEGKQHG DVVSAIKAGG DEAKLLVVDK ETDEFFKKCR VTPSQEHLDG 
       260        270        280        290        300
PLPEPFSNGE IQKENSREAL VEPASESPRP ALARSASSDT SEELNAQDSP 
       310        320        330        340        350
KRHDSTEPSS TSSSSDPILD FNISLAVAKE RAHQKRSSKR APQMDWSKKN 

ELFSNL

Length:356

Mass (Da):38,830

Last modified:January 23, 2007 - v3

Checksum:ⁱC562AEF5870B8E08

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF154336 mRNA Translation: AAF73258.1
NCBI Reference Sequences More...RefSeqⁱ	NP_067605.1, NM_021594.1
UniGeneⁱ	Rn.35142

Genome annotation databases

Ensemblⁱ	ENSRNOT00000004351; ENSRNOP00000004351; ENSRNOG00000003232
GeneIDⁱ	59114
KEGGⁱ	rno:59114

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9JJ19	Na(+)/H(+) exchange regulatory cofactor NHE-RF1		MOUSE		355	UniRef90_P70441
Na(+)/H(+) exchange regulatory cofactor NHE-RF		MESAU		354
Na(+)/H(+) exchange regulatory cofactor NHE-RF		CRIGR		354
UPI0004ED41DC		NANGA		356
UPI000A309381		MUSPA		356
+6

Protein	Similar proteins		Species	Score	Length	Source
Q9JJ19	Na(+)/H(+) exchange regulatory cofactor NHE-RF1		MOUSE		355	UniRef50_P70441
Na(+)/H(+) exchange regulatory cofactor NHE-RF1		CHICK		333
Na(+)/H(+) exchange regulatory cofactor NHE-RF		CRIGR		354
Na(+)/H(+) exchange regulatory cofactor NHE-RF		MESAU		354
Na(+)/H(+) exchange regulatory cofactor NHE-RF1		CHEMY		198
+34

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF154336 mRNA Translation: AAF73258.1
RefSeqⁱ	NP_067605.1, NM_021594.1
UniGeneⁱ	Rn.35142

3D structure databases

ProteinModelPortalⁱ	Q9JJ19
SMRⁱ	Q9JJ19
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	248735, 3 interactors
IntActⁱ	Q9JJ19, 1 interactor
STRINGⁱ	10116.ENSRNOP00000004351

PTM databases

iPTMnetⁱ	Q9JJ19
PhosphoSitePlusⁱ	Q9JJ19
SwissPalmⁱ	Q9JJ19

Proteomic databases

PaxDbⁱ	Q9JJ19
PRIDEⁱ	Q9JJ19

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000004351; ENSRNOP00000004351; ENSRNOG00000003232
GeneIDⁱ	59114
KEGGⁱ	rno:59114

Organism-specific databases

CTDⁱ	9368
RGDⁱ	708538 Slc9a3r1

Phylogenomic databases

eggNOGⁱ	ENOG410ITAB Eukaryota ENOG4110SZZ LUCA
GeneTreeⁱ	ENSGT00530000062999
HOGENOMⁱ	HOG000089940
HOVERGENⁱ	HBG052616
InParanoidⁱ	Q9JJ19
KOⁱ	K13365
OMAⁱ	LCAMKKG
OrthoDBⁱ	EOG091G086H
PhylomeDBⁱ	Q9JJ19
TreeFamⁱ	TF350449

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9JJ19

PROⁱ

PR:Q9JJ19

Gene expression databases

Bgeeⁱ	ENSRNOG00000003232 Expressed in 9 organ(s), highest expression level in adult mammalian kidney
Genevisibleⁱ	Q9JJ19 RN

Family and domain databases

InterProⁱ	View protein in InterPro IPR015098 EBP50_C-term IPR031199 NHERF-1 IPR017300 NHERF-1/NHERF-2 IPR001478 PDZ IPR036034 PDZ_sf
PANTHERⁱ	PTHR14191:SF7 PTHR14191:SF7, 1 hit
Pfamⁱ	View protein in Pfam PF09007 EBP50_C, 1 hit PF00595 PDZ, 2 hits
PIRSFⁱ	PIRSF037866 EBP50, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD283022 EBP50_C-term, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 2 hits
SUPFAMⁱ	SSF50156 SSF50156, 2 hits
PROSITEⁱ	View protein in PROSITE PS50106 PDZ, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NHRF1_RAT
Accessionⁱ	Q9JJ19Primary (citable) accession number: Q9JJ19
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
	Last sequence update:	January 23, 2007
	Last modified:	October 10, 2018
	This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9JJ19 (NHRF1_RAT)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Na(+)/H(+) exchange regulatory cofactor NHE-RF1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ