UniProtKB - Q9JJ19 (NHRF1_RAT)
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Slc9a3r1
Functioni
GO - Molecular functioni
- beta-2 adrenergic receptor binding Source: RGD
- beta-catenin binding Source: RGD
- chloride channel regulator activity Source: UniProtKB
- dopamine receptor binding Source: RGD
- growth factor receptor binding Source: RGD
- molecular adaptor activity Source: RGD
- myosin II binding Source: RGD
- PDZ domain binding Source: RGD
- phosphatase binding Source: RGD
- protein-containing complex binding Source: RGD
- protein domain specific binding Source: RGD
- protein-membrane adaptor activity Source: GO_Central
- protein N-terminus binding Source: RGD
- protein self-association Source: RGD
- signaling receptor binding Source: RGD
- type 2 metabotropic glutamate receptor binding Source: RGD
- type 3 metabotropic glutamate receptor binding Source: RGD
GO - Biological processi
- actin cytoskeleton organization Source: RGD
- adenylate cyclase-activating dopamine receptor signaling pathway Source: RGD
- auditory receptor cell stereocilium organization Source: RGD
- bile acid secretion Source: UniProtKB
- cAMP-mediated signaling Source: RGD
- cellular phosphate ion homeostasis Source: RGD
- cellular protein localization Source: RGD
- cerebrospinal fluid circulation Source: RGD
- cilium organization Source: RGD
- establishment of epithelial cell apical/basal polarity Source: RGD
- establishment of Golgi localization Source: RGD
- gamma-aminobutyric acid import Source: ARUK-UCL
- gland morphogenesis Source: RGD
- glutathione transport Source: UniProtKB
- import across plasma membrane Source: ARUK-UCL
- maintenance of epithelial cell apical/basal polarity Source: RGD
- microvillus assembly Source: RGD
- negative regulation of canonical Wnt signaling pathway Source: RGD
- negative regulation of cell migration Source: RGD
- negative regulation of cell motility Source: UniProtKB
- negative regulation of cell population proliferation Source: RGD
- negative regulation of ERK1 and ERK2 cascade Source: RGD
- negative regulation of mitotic cell cycle Source: RGD
- negative regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
- negative regulation of platelet-derived growth factor receptor signaling pathway Source: UniProtKB
- negative regulation of protein kinase B signaling Source: UniProtKB
- negative regulation of sodium:proton antiporter activity Source: RGD
- negative regulation of sodium ion transport Source: RGD
- nuclear migration Source: RGD
- phospholipase C-activating dopamine receptor signaling pathway Source: RGD
- plasma membrane organization Source: RGD
- positive regulation of intrinsic apoptotic signaling pathway Source: RGD
- positive regulation of ion transmembrane transport Source: RGD
- protein localization to plasma membrane Source: RGD
- regulation of cell shape Source: RGD
- regulation of cell size Source: RGD
- regulation of excretion Source: RGD
- regulation of protein kinase activity Source: UniProtKB
- renal absorption Source: UniProtKB
- renal phosphate ion absorption Source: UniProtKB
- renal sodium ion transport Source: RGD
- sensory perception of sound Source: RGD
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Biological process | Wnt signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1Short name: NHERF-1 Alternative name(s): Ezrin-radixin-moesin-binding phosphoprotein 50 Short name: EBP50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 |
Gene namesi | Name:Slc9a3r1 Synonyms:Nherf, Nherf1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708538, Slc9a3r1 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity
Other locations
- Cytoplasm By similarity
- filopodium By similarity
- ruffle By similarity
- microvillus By similarity
- Endomembrane system By similarity; Peripheral membrane protein By similarity
Note: Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Present in lipid rafts of T-cells. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity).By similarity
Extracellular region or secreted
- extracellular region Source: GOC
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- brush border membrane Source: RGD
- microvillus membrane Source: UniProtKB
- plasma membrane Source: RGD
- plasma membrane protein complex Source: ARUK-UCL
Other locations
- apical part of cell Source: RGD
- cell periphery Source: RGD
- cytoplasm Source: UniProtKB
- endomembrane system Source: UniProtKB-SubCell
- filopodium Source: UniProtKB-SubCell
- membrane Source: UniProtKB
- membrane raft Source: RGD
- microvillus Source: RGD
- perinuclear region of cytoplasm Source: RGD
- ruffle Source: UniProtKB-SubCell
- sperm midpiece Source: UniProtKB
- stereocilium Source: RGD
- stereocilium tip Source: RGD
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000096802 | 2 – 356 | Na(+)/H(+) exchange regulatory cofactor NHE-RF1Add BLAST | 355 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 2 | PhosphoserineCombined sources | 1 | |
Modified residuei | 46 | PhosphoserineBy similarity | 1 | |
Modified residuei | 266 | PhosphoserineBy similarity | 1 | |
Modified residuei | 277 | PhosphoserineCombined sources | 1 | |
Modified residuei | 287 | PhosphoserineCombined sources | 1 | |
Modified residuei | 288 | PhosphoserineCombined sources | 1 | |
Modified residuei | 290 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 291 | PhosphoserineCombined sources | 1 | |
Modified residuei | 299 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q9JJ19 |
PaxDbi | Q9JJ19 |
PRIDEi | Q9JJ19 |
PTM databases
iPTMneti | Q9JJ19 |
PhosphoSitePlusi | Q9JJ19 |
SwissPalmi | Q9JJ19 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000003232, Expressed in jejunum and 20 other tissues |
Genevisiblei | Q9JJ19, RN |
Interactioni
Subunit structurei
Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2, CFTR and PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Binds ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1, PLCB3, PDZK1 and CLCN3.
Forms a complex with CFTR and SLC4A7.
Forms a complex with SLC4A7 and ATP6V1B1 (By similarity).
Interacts with SLC4A7. Directly interacts with HTR4 (By similarity).
Interacts with TRPC4 (via the PDZ-binding domain) (By similarity).
Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity).
Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells.
Interacts with SLC34A1 (By similarity).
Interacts (via the PDZ domains) with SLC26A6 (By similarity).
Interacts with SLC26A3 (By similarity).
By similarityBinary interactionsi
Q9JJ19
With | #Exp. | IntAct |
---|---|---|
Asic3 [O35240] | 5 | EBI-982391,EBI-982374 |
GO - Molecular functioni
- beta-2 adrenergic receptor binding Source: RGD
- beta-catenin binding Source: RGD
- dopamine receptor binding Source: RGD
- growth factor receptor binding Source: RGD
- myosin II binding Source: RGD
- PDZ domain binding Source: RGD
- phosphatase binding Source: RGD
- protein domain specific binding Source: RGD
- protein N-terminus binding Source: RGD
- protein self-association Source: RGD
- signaling receptor binding Source: RGD
- type 2 metabotropic glutamate receptor binding Source: RGD
- type 3 metabotropic glutamate receptor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248735, 3 interactors |
IntActi | Q9JJ19, 2 interactors |
STRINGi | 10116.ENSRNOP00000004351 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 94 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 151 – 231 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 81 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3528, Eukaryota |
GeneTreei | ENSGT00950000182849 |
HOGENOMi | CLU_038627_1_0_1 |
InParanoidi | Q9JJ19 |
OMAi | QPKDRIV |
OrthoDBi | 880632at2759 |
PhylomeDBi | Q9JJ19 |
TreeFami | TF350449 |
Family and domain databases
Gene3Di | 2.30.42.10, 2 hits |
InterProi | View protein in InterPro IPR015098, EBP50_C-term IPR031199, NHERF-1 IPR017300, NHERF-1/NHERF-2 IPR001478, PDZ IPR036034, PDZ_sf |
PANTHERi | PTHR14191:SF7, PTHR14191:SF7, 1 hit |
Pfami | View protein in Pfam PF09007, EBP50_C, 1 hit PF00595, PDZ, 2 hits |
PIRSFi | PIRSF037866, EBP50, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 2 hits |
SUPFAMi | SSF50156, SSF50156, 2 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSADAAAGEP LPRLCCLEKG PNGYGFHLHG EKGKVGQFIR LVEPGSPAEK
60 70 80 90 100
SGLLAGDRLV EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLK
110 120 130 140 150
KLGVPIREEL LRAQEKSEHT EPPAAADTKK AGDQNEAEKS HLERCELRPR
160 170 180 190 200
LCTMKKGPNG YGFNLHSDKS KPGQFIRAVD PDSPAEASGL RAQDRIVEVN
210 220 230 240 250
GVCMEGKQHG DVVSAIKAGG DEAKLLVVDK ETDEFFKKCR VTPSQEHLDG
260 270 280 290 300
PLPEPFSNGE IQKENSREAL VEPASESPRP ALARSASSDT SEELNAQDSP
310 320 330 340 350
KRHDSTEPSS TSSSSDPILD FNISLAVAKE RAHQKRSSKR APQMDWSKKN
ELFSNL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF154336 mRNA Translation: AAF73258.1 |
RefSeqi | NP_067605.1, NM_021594.1 |
Genome annotation databases
Ensembli | ENSRNOT00000004351; ENSRNOP00000004351; ENSRNOG00000003232 |
GeneIDi | 59114 |
KEGGi | rno:59114 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF154336 mRNA Translation: AAF73258.1 |
RefSeqi | NP_067605.1, NM_021594.1 |
3D structure databases
SMRi | Q9JJ19 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248735, 3 interactors |
IntActi | Q9JJ19, 2 interactors |
STRINGi | 10116.ENSRNOP00000004351 |
PTM databases
iPTMneti | Q9JJ19 |
PhosphoSitePlusi | Q9JJ19 |
SwissPalmi | Q9JJ19 |
Proteomic databases
jPOSTi | Q9JJ19 |
PaxDbi | Q9JJ19 |
PRIDEi | Q9JJ19 |
Genome annotation databases
Ensembli | ENSRNOT00000004351; ENSRNOP00000004351; ENSRNOG00000003232 |
GeneIDi | 59114 |
KEGGi | rno:59114 |
Organism-specific databases
CTDi | 9368 |
RGDi | 708538, Slc9a3r1 |
Phylogenomic databases
eggNOGi | KOG3528, Eukaryota |
GeneTreei | ENSGT00950000182849 |
HOGENOMi | CLU_038627_1_0_1 |
InParanoidi | Q9JJ19 |
OMAi | QPKDRIV |
OrthoDBi | 880632at2759 |
PhylomeDBi | Q9JJ19 |
TreeFami | TF350449 |
Miscellaneous databases
PROi | PR:Q9JJ19 |
Gene expression databases
Bgeei | ENSRNOG00000003232, Expressed in jejunum and 20 other tissues |
Genevisiblei | Q9JJ19, RN |
Family and domain databases
Gene3Di | 2.30.42.10, 2 hits |
InterProi | View protein in InterPro IPR015098, EBP50_C-term IPR031199, NHERF-1 IPR017300, NHERF-1/NHERF-2 IPR001478, PDZ IPR036034, PDZ_sf |
PANTHERi | PTHR14191:SF7, PTHR14191:SF7, 1 hit |
Pfami | View protein in Pfam PF09007, EBP50_C, 1 hit PF00595, PDZ, 2 hits |
PIRSFi | PIRSF037866, EBP50, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 2 hits |
SUPFAMi | SSF50156, SSF50156, 2 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NHRF1_RAT | |
Accessioni | Q9JJ19Primary (citable) accession number: Q9JJ19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |