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Protein

N-acetyltransferase 8

Gene

Nat8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids. This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1. May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (By similarity).By similarity1 Publication

Catalytic activityi

Acetyl-CoA + an L-cysteine-S-conjugate = CoA + an N-acetyl L-cysteine-S-conjugate.

Pathwayi: glutathione metabolism

This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00204

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyltransferase 8 (EC:2.3.1.-)
Alternative name(s):
Acetyltransferase 2
Short name:
ATase2
Camello-like protein 4
Cysteinyl-conjugate N-acetyltransferase (EC:2.3.1.80)
Short name:
CCNAT
Gene namesi
Name:Nat8
Synonyms:Cml4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1915646 Nat8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicSequence analysisAdd BLAST35
Transmembranei36 – 56Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini57 – 227LumenalSequence analysisAdd BLAST171

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002846921 – 227N-acetyltransferase 8Add BLAST227

Proteomic databases

MaxQBiQ9JIY7
PaxDbiQ9JIY7
PeptideAtlasiQ9JIY7
PRIDEiQ9JIY7

PTM databases

iPTMnetiQ9JIY7
PhosphoSitePlusiQ9JIY7

Expressioni

Tissue specificityi

Expressed in brain (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030004
CleanExiMM_CML4
GenevisibleiQ9JIY7 MM

Interactioni

Subunit structurei

Interacts with PROM1. Interacts with BACE1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9JIY7, 2 interactors
MINTiQ9JIY7
STRINGi10090.ENSMUSP00000032073

Structurei

3D structure databases

ProteinModelPortaliQ9JIY7
SMRiQ9JIY7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 217N-acetyltransferasePROSITE-ProRule annotationAdd BLAST149

Sequence similaritiesi

Belongs to the camello family.1 Publication

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IS8N Eukaryota
ENOG410Y59A LUCA
GeneTreeiENSGT00390000009559
HOGENOMiHOG000261685
HOVERGENiHBG060476
InParanoidiQ9JIY7
KOiK20838
OMAiYHIRKYR
OrthoDBiEOG091G0LL0
PhylomeDBiQ9JIY7
TreeFamiTF324687

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 2 hits
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JIY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFRIRQFQ ERDYKQVVDV FSRGMEEHIP TAFRHLLTLP RTLLLLAVVP
60 70 80 90 100
LAIVLVSGSW FLAVVCIFFL FLFLWFLASK PWKNYVSKCL HTDMADITKS
110 120 130 140 150
YLSVRGSGFW VAESGGQVVG TVAARPVKDP PLGRKQLQLF RLSVSSQHRG
160 170 180 190 200
QGIAKALTRT VLQFARDQGY SDVVLVTGLL QQGAVTLYYS MGFQKTGESF
210 220
VDILTWLVDV SLIHFIYPLP SAQKYEL
Length:227
Mass (Da):25,638
Last modified:October 1, 2000 - v1
Checksum:iD93A2DFB1FB61C7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163317 mRNA Translation: AAF80486.1
AK002784 mRNA Translation: BAB22356.1
BC019517 mRNA Translation: AAH19517.1
CCDSiCCDS20301.1
RefSeqiNP_075944.1, NM_023455.3
XP_006506598.1, XM_006506535.3
XP_006506599.1, XM_006506536.2
XP_006506600.1, XM_006506537.3
UniGeneiMm.154782

Genome annotation databases

EnsembliENSMUST00000032073; ENSMUSP00000032073; ENSMUSG00000030004
GeneIDi68396
KEGGimmu:68396
UCSCiuc009cqh.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNAT8_MOUSE
AccessioniPrimary (citable) accession number: Q9JIY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 1, 2000
Last modified: July 18, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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