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Protein

Pleckstrin homology domain-containing family O member 1

Gene

Plekho1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei154Capping proteinBy similarity1
Binding sitei156Capping proteinBy similarity1

GO - Biological processi

  • lamellipodium morphogenesis Source: MGI
  • myoblast fusion Source: MGI
  • myoblast migration Source: MGI
  • regulation of cell shape Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family O member 1
Short name:
PH domain-containing family O member 1
Gene namesi
Name:Plekho1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1914470 Plekho1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003104241 – 408Pleckstrin homology domain-containing family O member 1Add BLAST408

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphoserineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei341PhosphoserineBy similarity1

Post-translational modificationi

C-terminal fragments could be released during apoptosis via caspase-3-dependent cleavage.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei309 – 310Cleavage; by caspase-3Sequence analysis2
Sitei344 – 345Cleavage; by caspase-3Sequence analysis2

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JIY0
PaxDbiQ9JIY0
PRIDEiQ9JIY0

PTM databases

iPTMnetiQ9JIY0
PhosphoSitePlusiQ9JIY0

Expressioni

Gene expression databases

BgeeiENSMUSG00000015745 Expressed in 238 organ(s), highest expression level in bone marrow macrophage
ExpressionAtlasiQ9JIY0 baseline and differential
GenevisibleiQ9JIY0 MM

Interactioni

Subunit structurei

Heterodimer or homodimer. Interacts with CK2 and actin capping subunits (capping protein CP-alpha and CP-beta). CKIP1 and CK2 together inhibit the activity of actin capping protein at the barbed ends of actin filaments. Interacts with ATM, IFP35, JUN, JUND, NMI and PI3K. Interacts with AKT1, AKT2 and AKT3 (each isozyme of PKB), PtdIns(3,5)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi212026, 5 interactors
STRINGi10090.ENSMUSP00000015889

Structurei

3D structure databases

ProteinModelPortaliQ9JIY0
SMRiQ9JIY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 131PHPROSITE-ProRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 192Interaction with capping proteins (CPs)By similarityAdd BLAST61
Regioni135 – 307Interaction with ATM, CKIP, IFP35 and NMIBy similarityAdd BLAST173
Regioni307 – 408Negative regulator of AP-1 activityBy similarityAdd BLAST102

Phylogenomic databases

eggNOGiENOG410IEJU Eukaryota
ENOG4110ACW LUCA
GeneTreeiENSGT00530000063760
HOGENOMiHOG000115563
HOVERGENiHBG108259
InParanoidiQ9JIY0
OMAiKRGPQDG
OrthoDBiEOG091G0CAS
PhylomeDBiQ9JIY0
TreeFamiTF333115

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR028452 CKIP-1
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR15871:SF1 PTHR15871:SF1, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9JIY0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKSGSGKRG PPDGNHQSAA PEKVGWVRKF CGKGIFREIW KNRYVVLKGD
60 70 80 90 100
QLYVSEKEVK DEKNSQEVFD LSDYEKCEEL RKSKSRSKKN HSKFTLARCR
110 120 130 140 150
QPGTTAPNLI FLAVSPEEKE SWINALSSAI TRAKNRILDE VTVEEDSYLA
160 170 180 190 200
HPTRDRAKIQ HSRRPPTRGH LMAVASTSTS DGMLTLDLIQ EEDPSPEEPA
210 220 230 240 250
SCAESFRVDL DKSVAQLAGS RRRADSDRIQ PSSQRASSLS RPWEKPDKGA
260 270 280 290 300
PYTPQALKKF PSTEKSRCAS LEEILSQRDT APARPLHLQA EESLPPVPAQ
310 320 330 340 350
PGQLSRIQDL VARKLEKTQE LLAEVQGLGD GKRKAKDPPQ SPPDSESEQL
360 370 380 390 400
LLETERLLGE ASSNWSQAKR VLQEVRELRD LYRQMDLQTP DSHLRQTSQH

SQYRKSLM
Length:408
Mass (Da):45,997
Last modified:October 1, 2000 - v1
Checksum:i33C36EC5DC8D4A72
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6XQM2F6XQM2_MOUSE
Pleckstrin homology domain-containi...
Plekho1
365Annotation score:
D3YVD1D3YVD1_MOUSE
Pleckstrin homology domain-containi...
Plekho1
124Annotation score:
F6VV25F6VV25_MOUSE
Pleckstrin homology domain-containi...
Plekho1
262Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116P → S in BAB29306 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF168675 mRNA Translation: AAF89643.1
AK014374 mRNA Translation: BAB29306.1
AK170025 mRNA Translation: BAE41517.1
AK170922 mRNA Translation: BAE42115.1
BC117003 mRNA Translation: AAI17004.1
BC125441 mRNA Translation: AAI25442.1
CCDSiCCDS17627.1
RefSeqiNP_075809.1, NM_023320.2
UniGeneiMm.458147

Genome annotation databases

EnsembliENSMUST00000015889; ENSMUSP00000015889; ENSMUSG00000015745
GeneIDi67220
KEGGimmu:67220
UCSCiuc008qlx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF168675 mRNA Translation: AAF89643.1
AK014374 mRNA Translation: BAB29306.1
AK170025 mRNA Translation: BAE41517.1
AK170922 mRNA Translation: BAE42115.1
BC117003 mRNA Translation: AAI17004.1
BC125441 mRNA Translation: AAI25442.1
CCDSiCCDS17627.1
RefSeqiNP_075809.1, NM_023320.2
UniGeneiMm.458147

3D structure databases

ProteinModelPortaliQ9JIY0
SMRiQ9JIY0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212026, 5 interactors
STRINGi10090.ENSMUSP00000015889

PTM databases

iPTMnetiQ9JIY0
PhosphoSitePlusiQ9JIY0

Proteomic databases

MaxQBiQ9JIY0
PaxDbiQ9JIY0
PRIDEiQ9JIY0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015889; ENSMUSP00000015889; ENSMUSG00000015745
GeneIDi67220
KEGGimmu:67220
UCSCiuc008qlx.2 mouse

Organism-specific databases

CTDi51177
MGIiMGI:1914470 Plekho1

Phylogenomic databases

eggNOGiENOG410IEJU Eukaryota
ENOG4110ACW LUCA
GeneTreeiENSGT00530000063760
HOGENOMiHOG000115563
HOVERGENiHBG108259
InParanoidiQ9JIY0
OMAiKRGPQDG
OrthoDBiEOG091G0CAS
PhylomeDBiQ9JIY0
TreeFamiTF333115

Miscellaneous databases

ChiTaRSiPlekho1 mouse
PROiPR:Q9JIY0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015745 Expressed in 238 organ(s), highest expression level in bone marrow macrophage
ExpressionAtlasiQ9JIY0 baseline and differential
GenevisibleiQ9JIY0 MM

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR028452 CKIP-1
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR15871:SF1 PTHR15871:SF1, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPKHO1_MOUSE
AccessioniPrimary (citable) accession number: Q9JIY0
Secondary accession number(s): Q9CXH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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