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Protein

Synaptic vesicle glycoprotein 2A

Gene

Sv2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
(Microbial infection) Receptor for C.botulinum neurotoxin type A (BoNT/A, botA); the toxin probably binds via extracellular loop 4 (PubMed:16543415).1 Publication
(Microbial infection) Possible receptor for C.botulinum neurotoxin type D (BoNT/D, botD); BoNT/D does not bind to extracellular loop 4 as do BoNT/A and BoNT/E (PubMed:21483489).1 Publication
(Microbial infection) Receptor for C.botulinum neurotoxin type E (BoNT/E); the toxin probably binds via extracellular loop 4 (PubMed:18815274). It probably requires glycosylation of Asn-573 (PubMed:18815274).1 Publication1 Publication

GO - Molecular functioni

  • protein kinase binding Source: ParkinsonsUK-UCL
  • transmembrane transporter activity Source: InterPro

GO - Biological processi

  • cellular calcium ion homeostasis Source: MGI
  • chemical synaptic transmission Source: MGI
  • regulation of gamma-aminobutyric acid secretion Source: MGI
  • synaptic vesicle priming Source: SynGO

Keywordsi

Molecular functionReceptor
Biological processNeurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicle glycoprotein 2A
Short name:
Synaptic vesicle protein 2
Short name:
Synaptic vesicle protein 2A
Alternative name(s):
Calcium regulator SV2A
Gene namesi
Name:Sv2a
Synonyms:Kiaa0736, Sv2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1927139 Sv2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 169CytoplasmicSequence analysisAdd BLAST169
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 205ExtracellularSequence analysisAdd BLAST15
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Topological domaini227 – 233CytoplasmicSequence analysis7
Transmembranei234 – 254HelicalSequence analysisAdd BLAST21
Topological domaini255 – 262ExtracellularSequence analysis8
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Topological domaini284 – 294CytoplasmicSequence analysisAdd BLAST11
Transmembranei295 – 315HelicalSequence analysisAdd BLAST21
Topological domaini316 – 334ExtracellularSequence analysisAdd BLAST19
Transmembranei335 – 355HelicalSequence analysisAdd BLAST21
Topological domaini356 – 447CytoplasmicSequence analysisAdd BLAST92
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 598ExtracellularSequence analysis1 PublicationAdd BLAST130
Transmembranei599 – 619HelicalSequence analysisAdd BLAST21
Topological domaini620 – 626CytoplasmicSequence analysis7
Transmembranei627 – 647HelicalSequence analysisAdd BLAST21
Topological domaini648 – 651ExtracellularSequence analysis4
Transmembranei652 – 672HelicalSequence analysisAdd BLAST21
Topological domaini673 – 685CytoplasmicSequence analysisAdd BLAST13
Transmembranei686 – 708HelicalSequence analysisAdd BLAST23
Topological domaini709 – 712ExtracellularSequence analysis4
Transmembranei713 – 731HelicalSequence analysisAdd BLAST19
Topological domaini732 – 742CytoplasmicSequence analysisAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice fail to grow, experience severe epileptic seizures and die immediately or shortly after birth probably due to multiple neural and endocrine deficits (PubMed:10624962). Mice lacking both Sv2a and Sv2b display a similar phenotype (PubMed:10624962). Diaphragm motor nerve terminals from mice with only a single copy of this gene and no Sv2b have decreased binding of C.botulinum neurotoxin type A (BoNT/A, botA) and are less sensitive to the toxin (PubMed:16543415). Hippocampal neurons from young mice lacking both Sv2a and Sv2b do not bind BoNT/A, nor do they take it up (PubMed:16543415, PubMed:18815274). Hippocampal neurons from young mice lacking both Sv2a and Sv2b do not bind C.botulinum neurotoxin type E (BoNT/E), nor do they take it up (PubMed:18815274). Hippocampal neurons from young mice lacking both Sv2a and Sv2b do not bind C.botulinum neurotoxin type BoNT/D (BoNT/D, botD), nor do they take it up (PubMed:21483489). Hippocampal neurons from young mice lacking both Sv2a and Sv2b take up C.botulinum neurotoxin type C (BoNT/C) and C.botulinum neurotoxin type F (BonT/F, botF) normally (PubMed:21483489).6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397661 – 742Synaptic vesicle glycoprotein 2AAdd BLAST742

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei80PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei84PhosphothreonineCombined sources1
Modified residuei127PhosphoserineCombined sources1
Modified residuei393PhosphoserineBy similarity1
Modified residuei480PhosphotyrosineCombined sources1
Glycosylationi498N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi548N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi573N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.By similarity
N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9JIS5
PaxDbiQ9JIS5
PeptideAtlasiQ9JIS5
PRIDEiQ9JIS5

PTM databases

iPTMnetiQ9JIS5
PhosphoSitePlusiQ9JIS5
SwissPalmiQ9JIS5

Expressioni

Tissue specificityi

Expressed in conventional synapses and cone ribbon synapses in the retina (at protein level) (PubMed:12687700). Expressed in diaphragm motor nerve terminals (at protein level) (PubMed:16543415). Expressed in hippocampus neurons (at protein level) (PubMed:18815274).3 Publications

Developmental stagei

Expressed during synaptogenesis in the retina (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000038486 Expressed in 237 organ(s), highest expression level in substantia nigra
CleanExiMM_SV2A
GenevisibleiQ9JIS5 MM

Interactioni

Subunit structurei

Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner. Binds the adapter protein complex AP-2 (By similarity).By similarity
(Microbial infection) Interacts with C.botulinum neurotoxin type A (BoNT/A, botA).1 Publication1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211019, 2 interactors
IntActiQ9JIS5, 5 interactors
MINTiQ9JIS5
STRINGi10090.ENSMUSP00000037576

Structurei

3D structure databases

ProteinModelPortaliQ9JIS5
SMRiQ9JIS5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 57Interaction with SYT1By similarityAdd BLAST57

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRID Eukaryota
ENOG410YQME LUCA
GeneTreeiENSGT00550000074384
HOGENOMiHOG000065727
HOVERGENiHBG053967
InParanoidiQ9JIS5
KOiK06258
OMAiMMMAVWF
OrthoDBiEOG091G02NC
PhylomeDBiQ9JIS5
TreeFamiTF324824

Family and domain databases

CDDicd06174 MFS, 2 hits
InterProiView protein in InterPro
IPR001646 5peptide_repeat
IPR011701 MFS
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
IPR022308 SV2
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
PF13599 Pentapeptide_4, 1 hit
PF00083 Sugar_tr, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR01299 synapt_SV2, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JIS5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEGFRDRAA FIRGAKDIAK EVKKHAAKKV VKGLDRVQDE YSRRSYSRFE
60 70 80 90 100
EEDDDDDFPA PADGYYRGEG AQDEEEGGAS SDATEGHDED DEIYEGEYQG
110 120 130 140 150
IPRAESGGKG ERMADGAPLA GVRGGLSDGE GPPGGRGEAQ RRKDREELAQ
160 170 180 190 200
QYETILRECG HGRFQWTLYF VLGLALMADG VEVFVVGFVL PSAEKDMCLS
210 220 230 240 250
DSNKGMLGLI VYLGMMVGAF LWGGLADRLG RRQCLLISLS VNSVFAFFSS
260 270 280 290 300
FVQGYGTFLF CRLLSGVGIG GSIPIVFSYF SEFLAQEKRG EHLSWLCMFW
310 320 330 340 350
MIGGVYAAAM AWAIIPHYGW SFQMGSAYQF HSWRVFVLVC AFPSVFAIGA
360 370 380 390 400
LTTQPESPRF FLENGKHDEA WMVLKQVHDT NMRAKGHPER VFSVTHIKTI
410 420 430 440 450
HQEDELIEIQ SDTGTWYQRW GVRALSLGGQ VWGNFLSCFS PEYRRITLMM
460 470 480 490 500
MGVWFTMSFS YYGLTVWFPD MIRHLQAVDY AARTKVFPGE RVEHVTFNFT
510 520 530 540 550
LENQIHRGGQ YFNDKFIGLR LKSVSFEDSL FEECYFEDVT SSNTFFRNCT
560 570 580 590 600
FINTVFYNTD LFEYKFVNSR LVNSTFLHNK EGCPLDVTGT GEGAYMVYFV
610 620 630 640 650
SFLGTLAVLP GNIVSALLMD KIGRLRMLAG SSVLSCVSCF FLSFGNSESA
660 670 680 690 700
MIALLCLFGG VSIASWNALD VLTVELYPSD KRTTAFGFLN ALCKLAAVLG
710 720 730 740
ISIFTSFVGI TKAAPILFAS AALALGSSLA LKLPETRGQV LQ
Length:742
Mass (Da):82,647
Last modified:October 1, 2000 - v1
Checksum:i1074857FD13ED894
GO

Sequence cautioni

The sequence BAC65642 differs from that shown. Reason: Frameshift at position 72.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196781, AF196780 Genomic DNA Translation: AAF87321.1
AK122360 Transcribed RNA Translation: BAC65642.3 Sequence problems.
AK028318 mRNA Translation: BAC25876.1
BC026494 mRNA Translation: AAH26494.1
BC046587 mRNA Translation: AAH46587.1
CCDSiCCDS17631.1
RefSeqiNP_071313.1, NM_022030.3
UniGeneiMm.491135

Genome annotation databases

EnsembliENSMUST00000035371; ENSMUSP00000037576; ENSMUSG00000038486
GeneIDi64051
KEGGimmu:64051
UCSCiuc008qmf.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196781, AF196780 Genomic DNA Translation: AAF87321.1
AK122360 Transcribed RNA Translation: BAC65642.3 Sequence problems.
AK028318 mRNA Translation: BAC25876.1
BC026494 mRNA Translation: AAH26494.1
BC046587 mRNA Translation: AAH46587.1
CCDSiCCDS17631.1
RefSeqiNP_071313.1, NM_022030.3
UniGeneiMm.491135

3D structure databases

ProteinModelPortaliQ9JIS5
SMRiQ9JIS5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211019, 2 interactors
IntActiQ9JIS5, 5 interactors
MINTiQ9JIS5
STRINGi10090.ENSMUSP00000037576

PTM databases

iPTMnetiQ9JIS5
PhosphoSitePlusiQ9JIS5
SwissPalmiQ9JIS5

Proteomic databases

MaxQBiQ9JIS5
PaxDbiQ9JIS5
PeptideAtlasiQ9JIS5
PRIDEiQ9JIS5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035371; ENSMUSP00000037576; ENSMUSG00000038486
GeneIDi64051
KEGGimmu:64051
UCSCiuc008qmf.1 mouse

Organism-specific databases

CTDi9900
MGIiMGI:1927139 Sv2a
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IRID Eukaryota
ENOG410YQME LUCA
GeneTreeiENSGT00550000074384
HOGENOMiHOG000065727
HOVERGENiHBG053967
InParanoidiQ9JIS5
KOiK06258
OMAiMMMAVWF
OrthoDBiEOG091G02NC
PhylomeDBiQ9JIS5
TreeFamiTF324824

Miscellaneous databases

PROiPR:Q9JIS5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038486 Expressed in 237 organ(s), highest expression level in substantia nigra
CleanExiMM_SV2A
GenevisibleiQ9JIS5 MM

Family and domain databases

CDDicd06174 MFS, 2 hits
InterProiView protein in InterPro
IPR001646 5peptide_repeat
IPR011701 MFS
IPR020846 MFS_dom
IPR005828 MFS_sugar_transport-like
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
IPR022308 SV2
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
PF13599 Pentapeptide_4, 1 hit
PF00083 Sugar_tr, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR01299 synapt_SV2, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSV2A_MOUSE
AccessioniPrimary (citable) accession number: Q9JIS5
Secondary accession number(s): Q80TT0, Q8R0R5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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