UniProtKB - Q9JIQ8 (TMPS2_MOUSE)
Protein
Transmembrane protease serine 2
Gene
Tmprss2
Organism
Mus musculus (Mouse)
Status
Functioni
Plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate. Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption (By similarity). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (PubMed:25734995).By similarity1 Publication
(Microbial infection) Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 294 | Charge relay systemBy similarity | 1 | |
Active sitei | 343 | Charge relay systemBy similarity | 1 | |
Active sitei | 439 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- scavenger receptor activity Source: InterPro
- serine-type endopeptidase activity Source: InterPro
GO - Biological processi
- positive regulation of viral entry into host cell Source: UniProtKB
- protein autoprocessing Source: UniProtKB
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Protein family/group databases
MEROPSi | S01.247 |
Names & Taxonomyi
Protein namesi | Recommended name: Transmembrane protease serine 2 (EC:3.4.21.-)Alternative name(s): Epitheliasin Plasmic transmembrane protein X Cleaved into the following 2 chains: |
Gene namesi | Name:Tmprss2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1354381, Tmprss2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Extracellular region or secreted
- Secreted By similarity
Note: Activated by cleavage and secreted.By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 83 | CytoplasmicSequence analysisAdd BLAST | 83 | |
Transmembranei | 84 – 104 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 105 – 490 | ExtracellularSequence analysisAdd BLAST | 386 |
Keywords - Cellular componenti
Cell membrane, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
Knockout mice show normal growth and reach normal adulthood without having abnormalities in organ histology and alteration in protein levels of prostatic secretions (PubMed:16428450). Abrogation of viral spread and protection of mice from severe pathology and death are observed after infection with influenza A virus strains H1N1 and H7N9.3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000027857 | 1 – 253 | Transmembrane protease serine 2 non-catalytic chainAdd BLAST | 253 | |
ChainiPRO_0000027858 | 254 – 490 | Transmembrane protease serine 2 catalytic chainAdd BLAST | 237 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 111 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 112 ↔ 125 | By similarity | ||
Disulfide bondi | 119 ↔ 138 | By similarity | ||
Disulfide bondi | 132 ↔ 147 | By similarity | ||
Disulfide bondi | 171 ↔ 230 | By similarity | ||
Disulfide bondi | 184 ↔ 240 | By similarity | ||
Glycosylationi | 212 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 243 ↔ 363 | Interchain (between non-catalytic and catalytic chains)PROSITE-ProRule annotation | ||
Disulfide bondi | 279 ↔ 295 | By similarity | ||
Disulfide bondi | 408 ↔ 424 | By similarity | ||
Disulfide bondi | 435 ↔ 463 | By similarity | ||
Glycosylationi | 474 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Proteolytically processed; by an autocatalytic mechanism.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 253 – 254 | CleavageSequence analysis | 2 |
Keywords - PTMi
Autocatalytic cleavage, Disulfide bond, Glycoprotein, ZymogenProteomic databases
MaxQBi | Q9JIQ8 |
PaxDbi | Q9JIQ8 |
PRIDEi | Q9JIQ8 |
PTM databases
GlyGeni | Q9JIQ8, 3 sites |
iPTMneti | Q9JIQ8 |
PhosphoSitePlusi | Q9JIQ8 |
Expressioni
Tissue specificityi
Larynx, trachea and bronchi, lung, prostate and kidney.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000000385, Expressed in paneth cell and 208 other tissues |
ExpressionAtlasi | Q9JIQ8, baseline and differential |
Genevisiblei | Q9JIQ8, MM |
Interactioni
Subunit structurei
The catalytically active form interacts with ACE2.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000000395 |
Miscellaneous databases
RNActi | Q9JIQ8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 111 – 149 | LDL-receptor class APROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 150 – 242 | SRCRPROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 254 – 487 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 234 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000155207 |
HOGENOMi | CLU_006842_19_2_1 |
InParanoidi | Q9JIQ8 |
OMAi | YQPESLY |
OrthoDBi | 1314811at2759 |
TreeFami | TF351678 |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit 3.10.250.10, 1 hit 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001190, SRCR IPR017448, SRCR-like_dom IPR036772, SRCR-like_dom_sf IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF15494, SRCR_2, 1 hit PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00202, SR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56487, SSF56487, 1 hit SSF57424, SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS50287, SRCR_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9JIQ8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALNSGSPPG IGPCYENHGY QSEHICPPRP PVAPNGYNLY PAQYYPSPVP
60 70 80 90 100
QYAPRITTQA STSVIHTHPK SSGALCTSKS KKSLCLALAL GTVLTGAAVA
110 120 130 140 150
AVLLWRFWDS NCSTSEMECG SSGTCISSSL WCDGVAHCPN GEDENRCVRL
160 170 180 190 200
YGQSFILQVY SSQRKAWYPV CQDDWSESYG RAACKDMGYK NNFYSSQGIP
210 220 230 240 250
DQSGATSFMK LNVSSGNVDL YKKLYHSDSC SSRMVVSLRC IECGVRSVKR
260 270 280 290 300
QSRIVGGLNA SPGDWPWQVS LHVQGVHVCG GSIITPEWIV TAAHCVEEPL
310 320 330 340 350
SSPRYWTAFA GILRQSLMFY GSRHQVEKVI SHPNYDSKTK NNDIALMKLQ
360 370 380 390 400
TPLAFNDLVK PVCLPNPGMM LDLDQECWIS GWGATYEKGK TSDVLNAAMV
410 420 430 440 450
PLIEPSKCNS KYIYNNLITP AMICAGFLQG SVDSCQGDSG GPLVTLKNGI
460 470 480 490
WWLIGDTSWG SGCAKALRPG VYGNVTVFTD WIYQQMRANS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A338P6L7 | A0A338P6L7_MOUSE | Transmembrane protease serine 2 | Tmprss2 | 96 | Annotation score: | ||
A0A338P779 | A0A338P779_MOUSE | Transmembrane protease serine 2 | Tmprss2 | 103 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | L → P in AAF97867 (PubMed:11169526).Curated | 1 | |
Sequence conflicti | 75 | L → P in AAF64186 (Ref. 2) Curated | 1 | |
Sequence conflicti | 75 | L → P in AAF21308 (PubMed:10683448).Curated | 1 | |
Sequence conflicti | 75 | L → P in AAH38393 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 122 | S → L in AAF21308 (PubMed:10683448).Curated | 1 | |
Sequence conflicti | 178 | S → N in AAF21308 (PubMed:10683448).Curated | 1 | |
Sequence conflicti | 302 | S → G in AAF97867 (PubMed:11169526).Curated | 1 | |
Sequence conflicti | 302 | S → G in AAF64186 (Ref. 2) Curated | 1 | |
Sequence conflicti | 302 | S → G in AAF21308 (PubMed:10683448).Curated | 1 | |
Sequence conflicti | 302 | S → G in AAH38393 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 320 | Y → H in AAF97867 (PubMed:11169526).Curated | 1 | |
Sequence conflicti | 474 | N → D in AAF97867 (PubMed:11169526).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF199362 mRNA Translation: AAF97867.1 AF243500 mRNA Translation: AAF64186.1 AF113596 mRNA Translation: AAF21308.1 BC038393 mRNA Translation: AAH38393.1 BC054348 mRNA Translation: AAH54348.1 |
CCDSi | CCDS37417.1 |
RefSeqi | NP_056590.2, NM_015775.2 XP_006523127.1, XM_006523064.3 XP_006523128.1, XM_006523065.2 |
Genome annotation databases
Ensembli | ENSMUST00000000395; ENSMUSP00000000395; ENSMUSG00000000385 |
GeneIDi | 50528 |
KEGGi | mmu:50528 |
UCSCi | uc008adl.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF199362 mRNA Translation: AAF97867.1 AF243500 mRNA Translation: AAF64186.1 AF113596 mRNA Translation: AAF21308.1 BC038393 mRNA Translation: AAH38393.1 BC054348 mRNA Translation: AAH54348.1 |
CCDSi | CCDS37417.1 |
RefSeqi | NP_056590.2, NM_015775.2 XP_006523127.1, XM_006523064.3 XP_006523128.1, XM_006523065.2 |
3D structure databases
SMRi | Q9JIQ8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000000395 |
Protein family/group databases
MEROPSi | S01.247 |
PTM databases
GlyGeni | Q9JIQ8, 3 sites |
iPTMneti | Q9JIQ8 |
PhosphoSitePlusi | Q9JIQ8 |
Proteomic databases
MaxQBi | Q9JIQ8 |
PaxDbi | Q9JIQ8 |
PRIDEi | Q9JIQ8 |
Protocols and materials databases
Antibodypediai | 2685, 293 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000000395; ENSMUSP00000000395; ENSMUSG00000000385 |
GeneIDi | 50528 |
KEGGi | mmu:50528 |
UCSCi | uc008adl.1, mouse |
Organism-specific databases
CTDi | 7113 |
MGIi | MGI:1354381, Tmprss2 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000155207 |
HOGENOMi | CLU_006842_19_2_1 |
InParanoidi | Q9JIQ8 |
OMAi | YQPESLY |
OrthoDBi | 1314811at2759 |
TreeFami | TF351678 |
Miscellaneous databases
BioGRID-ORCSi | 50528, 2 hits in 17 CRISPR screens |
ChiTaRSi | Tmprss2, mouse |
PROi | PR:Q9JIQ8 |
RNActi | Q9JIQ8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000385, Expressed in paneth cell and 208 other tissues |
ExpressionAtlasi | Q9JIQ8, baseline and differential |
Genevisiblei | Q9JIQ8, MM |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 1 hit 3.10.250.10, 1 hit 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001190, SRCR IPR017448, SRCR-like_dom IPR036772, SRCR-like_dom_sf IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF15494, SRCR_2, 1 hit PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00202, SR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56487, SSF56487, 1 hit SSF57424, SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS50287, SRCR_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TMPS2_MOUSE | |
Accessioni | Q9JIQ8Primary (citable) accession number: Q9JIQ8 Secondary accession number(s): Q7TN04, Q9JKC4, Q9QY82 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families