UniProtKB - Q9JIP7 (S15A1_MOUSE)
Protein
Solute carrier family 15 member 1
Gene
Slc15a1
Organism
Mus musculus (Mouse)
Status
Functioni
Proton-coupled amino-acid transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides (PubMed:11004485, PubMed:26320580). Primarily responsible for the absorption of dietary di- and tripeptides from the small intestinal lumen (By similarity).By similarity2 Publications
Catalytic activityi
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
GO - Molecular functioni
- channel inhibitor activity Source: MGI
- dipeptide transmembrane transporter activity Source: UniProtKB
- peptide:proton symporter activity Source: UniProtKB
- proton-dependent oligopeptide secondary active transmembrane transporter activity Source: MGI
GO - Biological processi
- dipeptide import across plasma membrane Source: UniProtKB
- negative regulation of amino acid transport Source: MGI
- oligopeptide transport Source: MGI
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Peptide transport, Protein transport, Symport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-427975, Proton/oligopeptide cotransporters |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 15 member 1CuratedAlternative name(s): Intestinal H(+)/peptide cotransporter1 Publication Oligopeptide transporter, small intestine isoform1 Publication Peptide transporter 11 Publication Proton-coupled dipeptide cotransporter |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1861376, Slc15a1 |
Subcellular locationi
Other locations
- Membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- apical plasma membrane Source: MGI
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: MGI
Other locations
- brush border Source: UniProtKB
- integral component of membrane Source: MGI
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 21 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 22 – 53 | ExtracellularSequence analysisAdd BLAST | 32 | |
Transmembranei | 54 – 74 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 75 – 82 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 83 – 103 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 104 – 118 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 119 – 139 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 140 – 161 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 162 – 182 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 183 – 198 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 199 – 219 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 220 – 276 | CytoplasmicSequence analysisAdd BLAST | 57 | |
Transmembranei | 277 – 297 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 298 – 327 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 328 – 348 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 349 – 361 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 362 – 382 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 383 – 585 | ExtracellularSequence analysisAdd BLAST | 203 | |
Transmembranei | 586 – 606 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 607 – 620 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 621 – 641 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 642 – 646 | ExtracellularSequence analysis | 5 | |
Transmembranei | 647 – 667 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 668 – 709 | CytoplasmicSequence analysisAdd BLAST | 42 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 453 – 456 | HDFE → AAFA or ERFR: Does not affect trypsin-binding. 1 Publication | 4 | |
Mutagenesisi | 472 – 475 | RVVK → AVVA or EVVE: Does not affect trypsin-binding. 1 Publication | 4 | |
Mutagenesisi | 476 | D → A: Does not affect trypsin-binding. 1 Publication | 1 | |
Mutagenesisi | 509 | E → R: Does not affect trypsin-binding. 1 Publication | 1 | |
Mutagenesisi | 515 | N → A: Does not affect trypsin-binding. 1 Publication | 1 | |
Mutagenesisi | 550 | D → A: Reduced trypsin-binding; when associated with A-573. 1 Publication | 1 | |
Mutagenesisi | 573 | E → A: Reduced trypsin-binding; when associated with A-550. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2073714 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064305 | 1 – 709 | Solute carrier family 15 member 1Add BLAST | 709 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 50 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 406 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 439 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 515 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 532 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q9JIP7 |
PaxDbi | Q9JIP7 |
PRIDEi | Q9JIP7 |
PTM databases
GlyGeni | Q9JIP7, 5 sites |
iPTMneti | Q9JIP7 |
PhosphoSitePlusi | Q9JIP7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025557, Expressed in jejunum and 90 other tissues |
Genevisiblei | Q9JIP7, MM |
Interactioni
Subunit structurei
Interacts (via extracellular domain region) with trypsin.
1 PublicationProtein-protein interaction databases
IntActi | Q9JIP7, 1 interactor |
STRINGi | 10090.ENSMUSP00000085728 |
Chemistry databases
BindingDBi | Q9JIP7 |
Miscellaneous databases
RNActi | Q9JIP7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9JIP7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 383 – 585 | Extracellular domain (ECD)1 PublicationAdd BLAST | 203 |
Domaini
The extracellular domain (ECD) region specifically binds trypsin.1 Publication
Sequence similaritiesi
Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1237, Eukaryota |
GeneTreei | ENSGT00940000155995 |
HOGENOMi | CLU_004790_3_0_1 |
InParanoidi | Q9JIP7 |
OMAi | KVAKCIG |
OrthoDBi | 365203at2759 |
TreeFami | TF330897 |
Family and domain databases
InterProi | View protein in InterPro IPR036259, MFS_trans_sf IPR004768, Oligopep_transport IPR000109, POT_fam IPR018456, PTR2_symporter_CS |
PANTHERi | PTHR11654, PTHR11654, 1 hit |
Pfami | View protein in Pfam PF00854, PTR2, 2 hits |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00926, 2A1704, 1 hit |
PROSITEi | View protein in PROSITE PS01022, PTR2_1, 1 hit PS01023, PTR2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9JIP7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGMSKSRGCF GYPLSIFFIV VNEFCERFSY YGMRALLVLY FRNFLGWDDN
60 70 80 90 100
LSTAIYHTFV ALCYLTPILG ALIADSWLGK FKTIVSLSIV YTIGQAVISV
110 120 130 140 150
SSINDLTDHD HNGSPDSLPV HVALSMVGLA LIALGTGGIK PCVSAFGGDQ
160 170 180 190 200
FEEGQEKQRN RFFSIFYLAI NGGSLLSTII TPILRVQQCG IHSQQACYPL
210 220 230 240 250
AFGVPAALMA VALIVFVLGS GMYKKFQPQG NIMGKVAKCI GFAIKNRFRH
260 270 280 290 300
RSKAYPKREH WLDWAKEKYD ERLISQIKMV TKVMFLYIPL PMFWALFDQQ
310 320 330 340 350
GSRWTLQATT MNGKIGAIEI QPDQMQTVNA ILIVIMVPIV DAVVYPLIAK
360 370 380 390 400
CGFNFTSLKK MTVGMFLASM AFVVAAIVQV EIDKTLPVFP GGNQVQIKVL
410 420 430 440 450
NIGNNNMTVH FPGNSVTLAQ MSQTDTFMTF DIDKLTSINI SSSGSPGVTT
460 470 480 490 500
VAHDFEQGHR HTLLVWNPSQ YRVVKDGLNQ KPEKGENGIR FVNTLNEMVT
510 520 530 540 550
IKMSGKVYEN VTSHNASGYQ FFPSGEKQYT INTTAVAPTC LTDFKSSNLD
560 570 580 590 600
FGSAYTYVIR RASDGCLEVK EFEDIPPNTV NMALQIPQYF LLTCGEVVFS
610 620 630 640 650
VTGLEFSYSQ APSNMKSVLQ AGWLLTVAVG NIIVLIVAGA GHFPKQWAEY
660 670 680 690 700
ILFASLLLVV CVIFAIMARF YTYINPAEIE AQFDEDEKKK GIGKENPYSS
LEPVSQTNM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 287 | Y → F in AAF81666 (PubMed:11004485).Curated | 1 | |
Sequence conflicti | 295 | A → G in AAF81666 (PubMed:11004485).Curated | 1 | |
Sequence conflicti | 318 | I → N in AAF81666 (PubMed:11004485).Curated | 1 | |
Sequence conflicti | 333 – 339 | IVIMVPI → NVNNGPN in AAF81666 (PubMed:11004485).Curated | 7 | |
Sequence conflicti | 346 – 347 | PL → RS in AAF81666 (PubMed:11004485).Curated | 2 | |
Sequence conflicti | 462 | T → N in AAF81666 (PubMed:11004485).Curated | 1 | |
Sequence conflicti | 467 | N → E in AAF81666 (PubMed:11004485).Curated | 1 | |
Sequence conflicti | 478 | L → P in AAF81666 (PubMed:11004485).Curated | 1 | |
Sequence conflicti | 501 | I → N in AAF81666 (PubMed:11004485).Curated | 1 | |
Sequence conflicti | 510 – 511 | NV → KF in AAF81666 (PubMed:11004485).Curated | 2 | |
Sequence conflicti | 520 – 522 | QFF → KFL in AAF81666 (PubMed:11004485).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF205540 mRNA Translation: AAF81666.1 AC126253 Genomic DNA No translation available. AC154618 Genomic DNA No translation available. |
CCDSi | CCDS37017.1 |
RefSeqi | NP_444309.2, NM_053079.2 |
Genome annotation databases
Ensembli | ENSMUST00000088386; ENSMUSP00000085728; ENSMUSG00000025557 |
GeneIDi | 56643 |
KEGGi | mmu:56643 |
UCSCi | uc007vad.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF205540 mRNA Translation: AAF81666.1 AC126253 Genomic DNA No translation available. AC154618 Genomic DNA No translation available. |
CCDSi | CCDS37017.1 |
RefSeqi | NP_444309.2, NM_053079.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5A9D | X-ray | 2.10 | A/B | 391-579 | [»] | |
SMRi | Q9JIP7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q9JIP7, 1 interactor |
STRINGi | 10090.ENSMUSP00000085728 |
Chemistry databases
BindingDBi | Q9JIP7 |
ChEMBLi | CHEMBL2073714 |
PTM databases
GlyGeni | Q9JIP7, 5 sites |
iPTMneti | Q9JIP7 |
PhosphoSitePlusi | Q9JIP7 |
Proteomic databases
MaxQBi | Q9JIP7 |
PaxDbi | Q9JIP7 |
PRIDEi | Q9JIP7 |
Protocols and materials databases
Antibodypediai | 10820, 224 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000088386; ENSMUSP00000085728; ENSMUSG00000025557 |
GeneIDi | 56643 |
KEGGi | mmu:56643 |
UCSCi | uc007vad.1, mouse |
Organism-specific databases
CTDi | 6564 |
MGIi | MGI:1861376, Slc15a1 |
Phylogenomic databases
eggNOGi | KOG1237, Eukaryota |
GeneTreei | ENSGT00940000155995 |
HOGENOMi | CLU_004790_3_0_1 |
InParanoidi | Q9JIP7 |
OMAi | KVAKCIG |
OrthoDBi | 365203at2759 |
TreeFami | TF330897 |
Enzyme and pathway databases
Reactomei | R-MMU-427975, Proton/oligopeptide cotransporters |
Miscellaneous databases
BioGRID-ORCSi | 56643, 0 hits in 17 CRISPR screens |
PROi | PR:Q9JIP7 |
RNActi | Q9JIP7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025557, Expressed in jejunum and 90 other tissues |
Genevisiblei | Q9JIP7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036259, MFS_trans_sf IPR004768, Oligopep_transport IPR000109, POT_fam IPR018456, PTR2_symporter_CS |
PANTHERi | PTHR11654, PTHR11654, 1 hit |
Pfami | View protein in Pfam PF00854, PTR2, 2 hits |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00926, 2A1704, 1 hit |
PROSITEi | View protein in PROSITE PS01022, PTR2_1, 1 hit PS01023, PTR2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S15A1_MOUSE | |
Accessioni | Q9JIP7Primary (citable) accession number: Q9JIP7 Secondary accession number(s): E9QMX3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families