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Protein

DNA repair protein RAD50

Gene

Rad50

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi681ZincPROSITE-ProRule annotation1
Metal bindingi684ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCell cycle, DNA damage, DNA repair, Meiosis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD50 (EC:3.6.-.-)
Gene namesi
Name:Rad50
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621542 Rad50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001386431 – 1312DNA repair protein RAD50Add BLAST1312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei635PhosphoserineBy similarity1
Modified residuei690PhosphothreonineBy similarity1
Modified residuei959N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JIL8
PRIDEiQ9JIL8

PTM databases

CarbonylDBiQ9JIL8
iPTMnetiQ9JIL8
PhosphoSitePlusiQ9JIL8

Expressioni

Tissue specificityi

Present at low levels in the heart at fetal-day 17, at relatively constant levels at postnatal days 10, 17 and 21 and at slightly lower levels in the adult heart. Detected in liver, kidney and lung. Barely detectable in skeletal muscle with slightly higher levels observed in brain and the ventricles of the heart (at protein level).1 Publication

Interactioni

Subunit structurei

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. Interacts with MRNIP.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248931, 1 interactor
STRINGi10116.ENSRNOP00000062378

Structurei

3D structure databases

ProteinModelPortaliQ9JIL8
SMRiQ9JIL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini635 – 734Zinc-hookPROSITE-ProRule annotationAdd BLAST100

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili200 – 534Sequence analysisAdd BLAST335
Coiled coili635 – 673Sequence analysisAdd BLAST39
Coiled coili706 – 734Sequence analysisAdd BLAST29
Coiled coili754 – 954Sequence analysisAdd BLAST201
Coiled coili1019 – 1075Sequence analysisAdd BLAST57

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1201 – 1238Ala/Asp-rich (DA-box)Add BLAST38

Domaini

The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11 homodimer (By similarity).By similarity

Sequence similaritiesi

Belongs to the SMC family. RAD50 subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0962 Eukaryota
COG0419 LUCA
HOGENOMiHOG000090195
HOVERGENiHBG058033
InParanoidiQ9JIL8
KOiK10866
PhylomeDBiQ9JIL8

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR038729 Rad50/SbcC_AAA
IPR004584 Rad50_eukaryotes
IPR013134 Zn_hook_RAD50
PfamiView protein in Pfam
PF13476 AAA_23, 1 hit
PF04423 Rad50_zn_hook, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00606 rad50, 1 hit
PROSITEiView protein in PROSITE
PS51131 ZN_HOOK, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9JIL8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRIEKMSTL GVRSFGIEDK DKQIISFFSP LTILVGPNGA GKTTIIECLK
60 70 80 90 100
YICTGDFPPG TKGNTFVHDP KVAQETDVRA QIRLQFRDVN GEMVLVQRSM
110 120 130 140 150
LCSQKSKKTE FKTLEGVITR IKHGEKVSLS SKCAEIDREM ISCLGVSKSV
160 170 180 190 200
LNNVIFCHQE DSNWPLSEGK ALKQKFDEIF SATRYIKALD TLRQVRQTQG
210 220 230 240 250
QKVKECQTEL KYLRQNKEKA CEIRDQITSK EAQLASSREI VKAYENELEP
260 270 280 290 300
LKNRLKEIEH NLSKIMRLDN EIKALDSRKK QMEKDNSELE QKMEKVFQGT
310 320 330 340 350
DEQLNDLYHN HQRTVREKER RLVDCQRELE KLSKEARLLN QERAELLVEQ
360 370 380 390 400
GRLQLQADRH QEHIRARDSL IQSLAAHLEL DGFERGPFSE RQIKNFHELV
410 420 430 440 450
RERQEREAKT ASQLLSDLTD KEALKQRQMD EMRDKKSGLG RMIELKTEIL
460 470 480 490 500
TKKQTELRNV RNELQQLEGS SDRILELDQE LTKAERELSK AEKNSSIETL
510 520 530 540 550
KAEILNLQSE KADLDRNLRK LDQEMEQLNH HTTTRTQMEM LTKDKTDKDE
560 570 580 590 600
QIRKIKSRHS DELTSLLGYF PNKKQLEDWL HSKSKEINQT RDRLAKLNKE
610 620 630 640 650
LASAEQNKNH INNELKKKEE QLSSYEDKLF DVCGSQDFES DLDRLKEDIE
660 670 680 690 700
KSSKQRAMLA GATAVYSQFI TQLTDENQSC CPGCQRVFQT EAELQEVISD
710 720 730 740 750
LQSKLRLAPD KLKSTESELK KKERRRDEML GLVPMRQSII DLKEKEIPEL
760 770 780 790 800
RNRLQSVNRD IQRLKNDIEE QETLLGTVMP EEESAKVCLT DVTIMERFQM
810 820 830 840 850
ELKDVERKIA QQAAKLQGVD LDRTVQQVNQ EKQEKQHKLD TVSSKIELNR
860 870 880 890 900
KLIQDQQEQI QHLKSKTNEL KSEKLQIATN LQRRQQMEEQ TVELSTEVQS
910 920 930 940 950
LNREIKDAKE QINPLEIALE KLQQEKEELI HRKNTSNKMA QDKINDIKEK
960 970 980 990 1000
VKNIHGYMKD IENYIQDGKD DYKKQKETEL NEVVIQLNEC DKHKEKINKE
1010 1020 1030 1040 1050
MGTMRQDIDT KKIQERWLQD NLTLRKRREE LKEVEEERKQ HLKEMGQMQV
1060 1070 1080 1090 1100
LQMKNEHQKL EENIDTIKRN HSLALGRQKG YEEEILHFKK ELREPQFRDA
1110 1120 1130 1140 1150
EEKYREMMIV MRTTELVNKD LDIYYKTLDH AIMKFHSMKM EEINKIIRDL
1160 1170 1180 1190 1200
WRSTYRGQDI EYIEIRSDAD ENVSASDKRR NYNYRVVMLK GDTALDMRGR
1210 1220 1230 1240 1250
CSAGQKVLAS LIIRLALAET FCLNCGILAL DEPTTNLDRE NIESLAHALV
1260 1270 1280 1290 1300
EIIKSRSQQR NFQLLVITHD EDFVELLGRS EYVEKFYRVK KNIDQCSEIV
1310
KSSINSLGSY VH
Length:1,312
Mass (Da):153,784
Last modified:October 1, 2000 - v1
Checksum:iF13C041BD2C05932
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V9X6G3V9X6_RAT
DNA repair protein RAD50
Rad50 rCG_34232
1,312Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218576 mRNA Translation: AAF91229.1
RefSeqiNP_071582.1, NM_022246.1
UniGeneiRn.51136

Genome annotation databases

GeneIDi64012
KEGGirno:64012
UCSCiRGD:621542 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218576 mRNA Translation: AAF91229.1
RefSeqiNP_071582.1, NM_022246.1
UniGeneiRn.51136

3D structure databases

ProteinModelPortaliQ9JIL8
SMRiQ9JIL8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248931, 1 interactor
STRINGi10116.ENSRNOP00000062378

PTM databases

CarbonylDBiQ9JIL8
iPTMnetiQ9JIL8
PhosphoSitePlusiQ9JIL8

Proteomic databases

PaxDbiQ9JIL8
PRIDEiQ9JIL8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64012
KEGGirno:64012
UCSCiRGD:621542 rat

Organism-specific databases

CTDi10111
RGDi621542 Rad50

Phylogenomic databases

eggNOGiKOG0962 Eukaryota
COG0419 LUCA
HOGENOMiHOG000090195
HOVERGENiHBG058033
InParanoidiQ9JIL8
KOiK10866
PhylomeDBiQ9JIL8

Miscellaneous databases

PROiPR:Q9JIL8

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR038729 Rad50/SbcC_AAA
IPR004584 Rad50_eukaryotes
IPR013134 Zn_hook_RAD50
PfamiView protein in Pfam
PF13476 AAA_23, 1 hit
PF04423 Rad50_zn_hook, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00606 rad50, 1 hit
PROSITEiView protein in PROSITE
PS51131 ZN_HOOK, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD50_RAT
AccessioniPrimary (citable) accession number: Q9JIL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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