UniProtKB - Q9JIL4 (NHRF3_MOUSE)
Na(+)/H(+) exchange regulatory cofactor NHE-RF3
Pdzk1
Functioni
A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity (By similarity).
Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na+-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
By similarity7 PublicationsMiscellaneous
GO - Molecular functioni
- PDZ domain binding Source: MGI
- protein-containing complex binding Source: MGI
- protein-membrane adaptor activity Source: GO_Central
- scavenger receptor binding Source: MGI
GO - Biological processi
- carnitine transport Source: MGI
- in utero embryonic development Source: MGI
- positive regulation of cation transmembrane transport Source: MGI
- positive regulation of ion transmembrane transporter activity Source: MGI
- positive regulation of protein localization to membrane Source: MGI
- positive regulation of protein targeting to membrane Source: UniProtKB
- protein localization to plasma membrane Source: UniProtKB
- regulation of anion transport Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3Short name: NHERF-3 Alternative name(s): CFTR-associated protein of 70 kDa Na(+)/H(+) exchanger regulatory factor 3 Na/Pi cotransporter C-terminal-associated protein 1 Short name: NaPi-Cap1 PDZ domain-containing protein 1 Sodium-hydrogen exchanger regulatory factor 3 |
Gene namesi | Name:Pdzk1 Synonyms:Cap70, Nherf3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1928901, Pdzk1 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications
Other locations
- Membrane ; Peripheral membrane protein By similarity
Note: Localized in the brush border membrane of renal proximal tubule cells (PubMed:11051556, PubMed:16141316). Associated with peripheral membranes (By similarity). Localizes to the apical compartment of proximal cells and to sinusoidal liver membranes (By similarity).By similarity2 Publications
Plasma Membrane
- apical plasma membrane Source: MGI
- brush border membrane Source: UniProtKB
- microvillus membrane Source: MGI
- plasma membrane Source: UniProtKB
Other locations
- brush border Source: MGI
- membrane raft Source: MGI
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | K → A: Impairs interaction of the first PDZ domain with SCARB1. 1 Publication | 1 | |
Mutagenesisi | 20 | Y → A: Disrupts interaction of the first PDZ domain with SCARB1. Abolishes interaction with SCARB1; when associated with A-253. 2 Publications | 1 | |
Mutagenesisi | 253 | Y → A: Disrupts interaction of the third PDZ domain with SCARB1. Abolishes interaction with SCARB1; when associated with A-20. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058288 | 1 – 519 | Na(+)/H(+) exchange regulatory cofactor NHE-RF3Add BLAST | 519 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 108 | PhosphoserineBy similarity | 1 | |
Modified residuei | 148 | PhosphoserineCombined sources | 1 | |
Modified residuei | 192 | PhosphoserineCombined sources | 1 | |
Modified residuei | 250 | PhosphoserineCombined sources | 1 | |
Modified residuei | 334 | PhosphoserineCombined sources | 1 | |
Modified residuei | 348 | PhosphoserineCombined sources | 1 | |
Modified residuei | 451 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 488 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 489 | PhosphoserineCombined sources | 1 | |
Modified residuei | 492 | PhosphoserineCombined sources | 1 | |
Modified residuei | 503 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 508 | PhosphoserineCombined sources | 1 | |
Modified residuei | 510 | PhosphoserineCombined sources | 1 | |
Modified residuei | 511 | PhosphoserineCombined sources | 1 | |
Modified residuei | 512 | PhosphoserineCombined sources | 1 | |
Modified residuei | 514 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9JIL4 |
MaxQBi | Q9JIL4 |
PaxDbi | Q9JIL4 |
PeptideAtlasi | Q9JIL4 |
PRIDEi | Q9JIL4 |
ProteomicsDBi | 252891 |
PTM databases
iPTMneti | Q9JIL4 |
PhosphoSitePlusi | Q9JIL4 |
SwissPalmi | Q9JIL4 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts with PDZK1IP1 and ABCC2. Binds to the C-terminal region of SLC26A3.
Interacts (via C-terminal PDZ domain) with SLC26A6 (via C-terminal domain).
Interacts (via C-terminal PDZ domain) with SLC9A3 (via C-terminal domain) (By similarity).
Component of a complex, composed of PDZK1, SYNGAP1, KLHL17 and NMDA receptors.
Interacts (via PDZ1 domain) directly with KLHL17; the interaction is important for integrity of actin cytoskeleton structures in neurons (By similarity).
Forms a heterodimeric complex with SLC9A3R1.
Interacts with AKAP2, BCR, CFTR, SLCO1A1, SLC22A12, SLC22A4, SLC22A5, SLC26A6, SLC9A3R2 and SLC17A1.
Interacts (via the first PDZ domain) with PTGIR (via non-isoprenylated C-terminus).
Interacts (via PDZ domains 1 and 3) with SCARB1 (C-terminal domain).
By similarity7 PublicationsGO - Molecular functioni
- PDZ domain binding Source: MGI
- scavenger receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 208488, 7 interactors |
CORUMi | Q9JIL4 |
IntActi | Q9JIL4, 5 interactors |
MINTi | Q9JIL4 |
STRINGi | 10090.ENSMUSP00000102685 |
Miscellaneous databases
RNActi | Q9JIL4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9JIL4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9JIL4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 90 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 128 – 215 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 243 – 323 | PDZ 3PROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 378 – 458 | PDZ 4PROSITE-ProRule annotationAdd BLAST | 81 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 348 – 374 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 479 – 519 | DisorderedSequence analysisAdd BLAST | 41 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 486 – 502 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 503 – 519 | Polar residuesSequence analysisAdd BLAST | 17 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3528, Eukaryota |
InParanoidi | Q9JIL4 |
OrthoDBi | 880632at2759 |
PhylomeDBi | Q9JIL4 |
TreeFami | TF350449 |
Family and domain databases
Gene3Di | 2.30.42.10, 4 hits |
InterProi | View protein in InterPro IPR001478, PDZ IPR036034, PDZ_sf |
Pfami | View protein in Pfam PF00595, PDZ, 4 hits |
SMARTi | View protein in SMART SM00228, PDZ, 4 hits |
SUPFAMi | SSF50156, SSF50156, 4 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 4 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASTFNPREC KLSKQEGQNY GFFLRIEKDT DGHLIRVIEE GSPAEKAGLL
60 70 80 90 100
DGDRVLRING VFVDKEEHAQ VVELVRKSGN SVTLLVLDGD SYEKAVKNQV
110 120 130 140 150
DLKELDQSQR EAALNDKKPG PGMNGAVEPC AQPRLCYLVK EGNSFGFSLK
160 170 180 190 200
TIQGKKGVYL TDIMPQGVAM KAGVLADDHL IEVNGENVEN ASHEEVVEKV
210 220 230 240 250
TKSGSRIMFL LVDKETARCH SEQKTQFKRE TASLKLLPHQ PRVVVIKKGS
260 270 280 290 300
NGYGFYLRAG PEQKGQIIKD IEPGSPAEAA GLKNNDLVVA VNGKSVEALD
310 320 330 340 350
HDGVVEMIRK GGDQTTLLVL DKEAESIYSL ARFSPLLYCQ SQELPNGSVK
360 370 380 390 400
EGPAPIPAPL EATGSEPTED AEGHKPKLCR LLKEDDSYGF HLNAIRGQPG
410 420 430 440 450
SFVKEVQQGG PADKAGLENE DVIIEVNGEN VQEEPYDRVV ERIKSSGKHV
460 470 480 490 500
TLLVCGKMAY SYFQAKKIPI VSSMAEPLVA GPDEKGETSA ESEHDAHPAK
510
DRTLSTASHS SSNSEDTEM
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1V0 | A0A0R4J1V0_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Pdzk1 | 519 | Annotation score: | ||
D3YW13 | D3YW13_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Pdzk1 | 125 | Annotation score: | ||
D3YZS9 | D3YZS9_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Pdzk1 | 153 | Annotation score: | ||
D3Z114 | D3Z114_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Pdzk1 | 50 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | L → R in AAL84634 (PubMed:11051556).Curated | 1 | |
Sequence conflicti | 135 | L → S in AAL84634 (PubMed:11051556).Curated | 1 | |
Sequence conflicti | 162 | D → N in BAB24493 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 162 | D → N in BAC26598 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 162 | D → N in BAC26605 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 162 | D → N in BAB28007 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 162 | D → N in BAB29600 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 162 | D → S in AAL84634 (PubMed:11051556).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF474247 mRNA Translation: AAL84634.1 Frameshift. AF220100 mRNA Translation: AAF73863.1 AK006269 mRNA Translation: BAB24493.1 AK029752 mRNA Translation: BAC26598.1 AK029764 mRNA Translation: BAC26605.1 Frameshift. AK012070 mRNA Translation: BAB28007.1 AK014879 mRNA Translation: BAB29600.1 BC013512 mRNA Translation: AAH13512.1 |
CCDSi | CCDS17649.1 |
RefSeqi | NP_001139473.1, NM_001146001.1 NP_067492.2, NM_021517.2 XP_006501896.1, XM_006501833.2 XP_011238480.1, XM_011240178.2 XP_017175164.1, XM_017319675.1 |
Genome annotation databases
GeneIDi | 59020 |
KEGGi | mmu:59020 |
UCSCi | uc008qoi.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF474247 mRNA Translation: AAL84634.1 Frameshift. AF220100 mRNA Translation: AAF73863.1 AK006269 mRNA Translation: BAB24493.1 AK029752 mRNA Translation: BAC26598.1 AK029764 mRNA Translation: BAC26605.1 Frameshift. AK012070 mRNA Translation: BAB28007.1 AK014879 mRNA Translation: BAB29600.1 BC013512 mRNA Translation: AAH13512.1 |
CCDSi | CCDS17649.1 |
RefSeqi | NP_001139473.1, NM_001146001.1 NP_067492.2, NM_021517.2 XP_006501896.1, XM_006501833.2 XP_011238480.1, XM_011240178.2 XP_017175164.1, XM_017319675.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2D90 | NMR | - | A | 242-330 | [»] | |
2EDZ | NMR | - | A | 1-107 | [»] | |
3NGH | X-ray | 1.80 | A/B | 7-106 | [»] | |
3R68 | X-ray | 1.30 | A | 238-323 | [»] | |
3R69 | X-ray | 1.50 | A/B | 241-322 | [»] | |
4F8K | X-ray | 1.70 | A/B | 7-106 | [»] | |
4R2Z | X-ray | 1.70 | A/B | 375-459 | [»] | |
SMRi | Q9JIL4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 208488, 7 interactors |
CORUMi | Q9JIL4 |
IntActi | Q9JIL4, 5 interactors |
MINTi | Q9JIL4 |
STRINGi | 10090.ENSMUSP00000102685 |
PTM databases
iPTMneti | Q9JIL4 |
PhosphoSitePlusi | Q9JIL4 |
SwissPalmi | Q9JIL4 |
Proteomic databases
jPOSTi | Q9JIL4 |
MaxQBi | Q9JIL4 |
PaxDbi | Q9JIL4 |
PeptideAtlasi | Q9JIL4 |
PRIDEi | Q9JIL4 |
ProteomicsDBi | 252891 |
Protocols and materials databases
DNASUi | 59020 |
Genome annotation databases
GeneIDi | 59020 |
KEGGi | mmu:59020 |
UCSCi | uc008qoi.2, mouse |
Organism-specific databases
CTDi | 5174 |
MGIi | MGI:1928901, Pdzk1 |
Phylogenomic databases
eggNOGi | KOG3528, Eukaryota |
InParanoidi | Q9JIL4 |
OrthoDBi | 880632at2759 |
PhylomeDBi | Q9JIL4 |
TreeFami | TF350449 |
Miscellaneous databases
BioGRID-ORCSi | 59020, 2 hits in 64 CRISPR screens |
ChiTaRSi | Pdzk1, mouse |
EvolutionaryTracei | Q9JIL4 |
PROi | PR:Q9JIL4 |
RNActi | Q9JIL4, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 2.30.42.10, 4 hits |
InterProi | View protein in InterPro IPR001478, PDZ IPR036034, PDZ_sf |
Pfami | View protein in Pfam PF00595, PDZ, 4 hits |
SMARTi | View protein in SMART SM00228, PDZ, 4 hits |
SUPFAMi | SSF50156, SSF50156, 4 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NHRF3_MOUSE | |
Accessioni | Q9JIL4Primary (citable) accession number: Q9JIL4 Secondary accession number(s): Q8CDP5, Q8R4G2, Q9CQ72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families