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Protein

Coatomer subunit beta

Gene

Copb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1 (By similarity). Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins in cortical neurons. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis.By similarity2 Publications

Miscellaneous

Brefeldin A induces dissociation from the Golgi of the beta-COP and presumably the other coatomer subunits.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit beta
Alternative name(s):
Beta-coat protein
Short name:
Beta-COP
Gene namesi
Name:Copb1
Synonyms:Copb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1917599 Copb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001938342 – 953Coatomer subunit betaAdd BLAST952

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei494N6-acetyllysineCombined sources1

Post-translational modificationi

Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8.

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9JIF7
MaxQBiQ9JIF7
PaxDbiQ9JIF7
PeptideAtlasiQ9JIF7
PRIDEiQ9JIF7

PTM databases

iPTMnetiQ9JIF7
PhosphoSitePlusiQ9JIF7
SwissPalmiQ9JIF7

Expressioni

Tissue specificityi

Predominantly expressed in the upper one-third of the oxyntic mucosa and in most regions of the pyloric mucosa (PubMed:16476741). Ubiquitiously expressed including platelet, liver, heart, spleen, lung and kidney (PubMed:11441537).2 Publications

Gene expression databases

BgeeiENSMUSG00000030754 Expressed in 303 organ(s), highest expression level in ureter smooth muscle
CleanExiMM_COPB1
GenevisibleiQ9JIF7 MM

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits (By similarity). Interacts with CAPN8. Interacts with SCYL1 and PRKCE (By similarity). Interacts with COPG1 (By similarity). Interacts with ARF1 (myristoylated); this interaction is required for binding of COPB1 to Golgi membranes (By similarity). Interacts (via trunk domain) with ARF1 (via switch I region); the interaction is direct (By similarity). Interacts with KCNK2 (via N-terminus); this interaction increases the channel-mediated whole cell currents and promotes plasma membrane expression of KCNK2 (By similarity). Interacts with STX17 (By similarity). Interacts with TMEM115 (By similarity).By similarity

Protein-protein interaction databases

BioGridi213992, 5 interactors
CORUMiQ9JIF7
DIPiDIP-42642N
IntActiQ9JIF7, 10 interactors
MINTiQ9JIF7
STRINGi10090.ENSMUSP00000033012

Structurei

3D structure databases

ProteinModelPortaliQ9JIF7
SMRiQ9JIF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati96 – 131HEAT 1Add BLAST36
Repeati132 – 168HEAT 2Add BLAST37
Repeati240 – 276HEAT 3Add BLAST37
Repeati277 – 314HEAT 4Add BLAST38
Repeati316 – 353HEAT 5Add BLAST38
Repeati396 – 433HEAT 6Add BLAST38

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1058 Eukaryota
COG5096 LUCA
GeneTreeiENSGT00390000005270
HOGENOMiHOG000207417
HOVERGENiHBG005380
InParanoidiQ9JIF7
KOiK17301
OMAiQHPNEFL
OrthoDBiEOG091G03TX
PhylomeDBiQ9JIF7
TreeFamiTF105737

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
IPR011710 Coatomer_bsu_C
IPR016460 COPB1
IPR029446 COPB1_appendage_platform_dom
PANTHERiPTHR10635 PTHR10635, 1 hit
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF07718 Coatamer_beta_C, 1 hit
PF14806 Coatomer_b_Cpla, 1 hit
PIRSFiPIRSF005727 Coatomer_beta_subunit, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JIF7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAAENVCYT LINVPMDSEP PSEISLKNDL EKGDVKSKTE ALKKVIIMIL
60 70 80 90 100
NGEKLPGLLM TIIRFVLPLQ DHTIKKLLLV FWEIVPKTTP DGRLLHEMIL
110 120 130 140 150
VCDAYRKDLQ HPNEFIRGST LRFLCKLKEA ELLEPLMPAI RACLEHRHSY
160 170 180 190 200
VRRNAVLAIY TIYRNFEHLI PDAPELIHDF LVNEKDASCK RNAFMMLIHA
210 220 230 240 250
DQDRALDYLS TCIDQVQTFG DILQLVIVEL IYKVCHANPS ERARFIRCIY
260 270 280 290 300
NLLQSSSPAV KYEAAGTLVT LSSAPTAIKA AAQCYIDLII KESDNNVKLI
310 320 330 340 350
VLDRLVELKE HPAHERVLQD LVMDILRVLS TPDLEVRKKT LQLALDLVSS
360 370 380 390 400
RNVEELVIVL KKEVIKTNNV SEHEDTDKYR QLLVRTLHSC SVRFPDMAAN
410 420 430 440 450
VIPVLMEFLS DSNEAAAADV LEFVREAIQR FDNLRMLIVE KMLEVFHAIK
460 470 480 490 500
SVKIYRGALW ILGEYCSTKE DIQSVMTEVR RSLGEIPIVE SEIKKEAGEL
510 520 530 540 550
KPEEEITVGP VQKLVTEMGT YATQSALSSS RPTKKEEDRP PLRGFLLDGD
560 570 580 590 600
FFVAASLATT LTKIALRYVA LVQEKKKQNS FVAEAMLLMA TILHLGKSSL
610 620 630 640 650
PKKPITDDDV DRISLCLKVL SECSPLMNDI FNKECRQSLS QMLSAKLEEE
660 670 680 690 700
KLSQKKESEK RNVTVQPDDP ISFMQLTAKN EMNCKEDQFQ LSLLAAMGNT
710 720 730 740 750
QRKEAADPLA SKLNKVTQLT GFSDPVYAEA YVHVNQYDIV LDVLVVNQTS
760 770 780 790 800
DTLQNCTLEL ATLGDLKLVE KPSPLTLAPH DFANIKANVK VASTENGIIF
810 820 830 840 850
GNIVYDVSGA ASDRNCVVLS DIHIDIMDYI QPATCTDAEF RQMWAEFEWE
860 870 880 890 900
NKVTVNTNMT DLNDYLQHIL KSTNMKCLTP EKALSGYCGF MAANLYARSI
910 920 930 940 950
FGEDALANVS IEKPVHQGPD AAVTGHIRIR AKSQGMALSL GDKINLSQKK

TSL
Length:953
Mass (Da):107,066
Last modified:October 1, 2000 - v1
Checksum:i4CD5407AD87ACB4B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti757T → I in BAE29109 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF231925 mRNA Translation: AAF76856.1
AK149828 mRNA Translation: BAE29109.1
AK152222 mRNA Translation: BAE31049.1
AK161544 mRNA Translation: BAE36453.1
AK172214 mRNA Translation: BAE42886.1
CH466531 Genomic DNA Translation: EDL17060.1
BC030837 mRNA Translation: AAH30837.1
CCDSiCCDS21759.1
RefSeqiNP_203534.1, NM_033370.3
UniGeneiMm.277024

Genome annotation databases

EnsembliENSMUST00000033012; ENSMUSP00000033012; ENSMUSG00000030754
GeneIDi70349
KEGGimmu:70349
UCSCiuc009jhx.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF231925 mRNA Translation: AAF76856.1
AK149828 mRNA Translation: BAE29109.1
AK152222 mRNA Translation: BAE31049.1
AK161544 mRNA Translation: BAE36453.1
AK172214 mRNA Translation: BAE42886.1
CH466531 Genomic DNA Translation: EDL17060.1
BC030837 mRNA Translation: AAH30837.1
CCDSiCCDS21759.1
RefSeqiNP_203534.1, NM_033370.3
UniGeneiMm.277024

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5A1Uelectron microscopy13.00G1-953[»]
5A1Velectron microscopy21.00G/O/X1-953[»]
5A1Welectron microscopy18.00G1-953[»]
5A1Xelectron microscopy23.00G/O1-953[»]
5A1Yelectron microscopy21.00G/O1-953[»]
5NZRelectron microscopy9.20B1-953[»]
5NZSelectron microscopy10.10B1-953[»]
5NZTelectron microscopy17.00B/N1-953[»]
5NZUelectron microscopy15.00B1-953[»]
5NZVelectron microscopy17.30B/I1-953[»]
ProteinModelPortaliQ9JIF7
SMRiQ9JIF7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213992, 5 interactors
CORUMiQ9JIF7
DIPiDIP-42642N
IntActiQ9JIF7, 10 interactors
MINTiQ9JIF7
STRINGi10090.ENSMUSP00000033012

PTM databases

iPTMnetiQ9JIF7
PhosphoSitePlusiQ9JIF7
SwissPalmiQ9JIF7

Proteomic databases

EPDiQ9JIF7
MaxQBiQ9JIF7
PaxDbiQ9JIF7
PeptideAtlasiQ9JIF7
PRIDEiQ9JIF7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033012; ENSMUSP00000033012; ENSMUSG00000030754
GeneIDi70349
KEGGimmu:70349
UCSCiuc009jhx.1 mouse

Organism-specific databases

CTDi1315
MGIiMGI:1917599 Copb1

Phylogenomic databases

eggNOGiKOG1058 Eukaryota
COG5096 LUCA
GeneTreeiENSGT00390000005270
HOGENOMiHOG000207417
HOVERGENiHBG005380
InParanoidiQ9JIF7
KOiK17301
OMAiQHPNEFL
OrthoDBiEOG091G03TX
PhylomeDBiQ9JIF7
TreeFamiTF105737

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Miscellaneous databases

ChiTaRSiCopb1 mouse
PROiPR:Q9JIF7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030754 Expressed in 303 organ(s), highest expression level in ureter smooth muscle
CleanExiMM_COPB1
GenevisibleiQ9JIF7 MM

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
IPR011710 Coatomer_bsu_C
IPR016460 COPB1
IPR029446 COPB1_appendage_platform_dom
PANTHERiPTHR10635 PTHR10635, 1 hit
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF07718 Coatamer_beta_C, 1 hit
PF14806 Coatomer_b_Cpla, 1 hit
PIRSFiPIRSF005727 Coatomer_beta_subunit, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCOPB_MOUSE
AccessioniPrimary (citable) accession number: Q9JIF7
Secondary accession number(s): Q3T9Y4
, Q3TT72, Q3U8G9, Q3UE02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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