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Protein

General transcription factor IIH subunit 2

Gene

Gtf2h2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri292 – 309C4-typeAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-72086 mRNA Capping
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 2
Alternative name(s):
Basic transcription factor 2 44 kDa subunit
Short name:
BTF2 p44
General transcription factor IIH polypeptide 2
TFIIH basal transcription factor complex p44 subunit
Gene namesi
Name:Gtf2h2
Synonyms:Btf2p44
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1345669 Gtf2h2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192491 – 396General transcription factor IIH subunit 2Add BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JIB4
MaxQBiQ9JIB4
PaxDbiQ9JIB4
PRIDEiQ9JIB4

PTM databases

iPTMnetiQ9JIB4
PhosphoSitePlusiQ9JIB4

Expressioni

Gene expression databases

BgeeiENSMUSG00000021639 Expressed in 269 organ(s), highest expression level in embryonic stem cell
CleanExiMM_GTF2H2
ExpressionAtlasiQ9JIB4 baseline and differential
GenevisibleiQ9JIB4 MM

Interactioni

Subunit structurei

Component of the TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2 and ERCC3. Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with XPB, XPD, GTF2H1 and GTF2H3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204782, 3 interactors
IntActiQ9JIB4, 1 interactor
STRINGi10090.ENSMUSP00000065228

Structurei

3D structure databases

ProteinModelPortaliQ9JIB4
SMRiQ9JIB4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 236VWFAPROSITE-ProRule annotationAdd BLAST177

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi242 – 245Poly-Ser4

Sequence similaritiesi

Belongs to the GTF2H2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri292 – 309C4-typeAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2807 Eukaryota
COG5151 LUCA
GeneTreeiENSGT00490000043395
HOGENOMiHOG000159415
HOVERGENiHBG059468
InParanoidiQ9JIB4
KOiK03142
OrthoDBiEOG091G08ZR
PhylomeDBiQ9JIB4
TreeFamiTF314037

Family and domain databases

CDDicd01453 vWA_transcription_factor_IIH_t, 1 hit
Gene3Di3.30.40.10, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR007198 Ssl1-like
IPR004595 TFIIH_C1-like_dom
IPR012170 TFIIH_SSL1/p44
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
IPR013087 Znf_C2H2_type
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12695 PTHR12695, 1 hit
PfamiView protein in Pfam
PF07975 C1_4, 1 hit
PF04056 Ssl1, 1 hit
PIRSFiPIRSF015919 TFIIH_SSL1, 1 hit
SMARTiView protein in SMART
SM01047 C1_4, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
TIGRFAMsiTIGR00622 ssl1, 1 hit
PROSITEiView protein in PROSITE
PS50234 VWFA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q9JIB4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEEPERTKR WEGGYERTWE ILKEDETGSL KATIEDILFK AKRKRVFEHH
60 70 80 90 100
GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLEY FVEEYFDQNP
110 120 130 140 150
ISQIGIIVTK SKRAEKLTEL SGNPRKHITS LKKAVDMTCH GEPSLYNSLS
160 170 180 190 200
MAMQTLKHMP GHTSREVLII FSSLTTCDPS NIYDLIKTLK TAKIRVSVIG
210 220 230 240 250
LSAEVRVCTV LARETGGTYH VILDETHYKE LLAHHVSPPP ASSSSECSLI
260 270 280 290 300
RMGFPQHTIA SLSDQDAKPS FSMAHLDNNS TEPGLTLGGY FCPQCRAKYC
310 320 330 340 350
ELPVECKICG LTLVSAPHLA RSYHHLFPLD AFQEISLEEY KGERFCYGCQ
360 370 380 390
GELKDQHVYV CTVCQNVFCV DCDVFVHDSL HCCPGCIHKI PTPSGI
Length:396
Mass (Da):44,687
Last modified:October 1, 2000 - v1
Checksum:i1BC57AFD464AA83F
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q91YN8Q91YN8_MOUSE
General transcription factor IIH su...
Gtf2h2 mCG_116162
398Annotation score:
Q8C5I2Q8C5I2_MOUSE
General transcription factor IIH su...
Gtf2h2
201Annotation score:
S4R2R0S4R2R0_MOUSE
General transcription factor II H, ...
Gtf2h2 mCG_116162
159Annotation score:
A0A338P6R6A0A338P6R6_MOUSE
General transcription factor IIH su...
Gtf2h2
120Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242432 Genomic DNA Translation: AAF82753.1
CCDSiCCDS26730.1
RefSeqiNP_071294.3, NM_022011.4
UniGeneiMm.272533

Genome annotation databases

EnsembliENSMUST00000066984; ENSMUSP00000065228; ENSMUSG00000021639
GeneIDi23894
KEGGimmu:23894
UCSCiuc007rqu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242432 Genomic DNA Translation: AAF82753.1
CCDSiCCDS26730.1
RefSeqiNP_071294.3, NM_022011.4
UniGeneiMm.272533

3D structure databases

ProteinModelPortaliQ9JIB4
SMRiQ9JIB4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204782, 3 interactors
IntActiQ9JIB4, 1 interactor
STRINGi10090.ENSMUSP00000065228

PTM databases

iPTMnetiQ9JIB4
PhosphoSitePlusiQ9JIB4

Proteomic databases

EPDiQ9JIB4
MaxQBiQ9JIB4
PaxDbiQ9JIB4
PRIDEiQ9JIB4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066984; ENSMUSP00000065228; ENSMUSG00000021639
GeneIDi23894
KEGGimmu:23894
UCSCiuc007rqu.2 mouse

Organism-specific databases

CTDi2966
MGIiMGI:1345669 Gtf2h2

Phylogenomic databases

eggNOGiKOG2807 Eukaryota
COG5151 LUCA
GeneTreeiENSGT00490000043395
HOGENOMiHOG000159415
HOVERGENiHBG059468
InParanoidiQ9JIB4
KOiK03142
OrthoDBiEOG091G08ZR
PhylomeDBiQ9JIB4
TreeFamiTF314037

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-72086 mRNA Capping
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Miscellaneous databases

ChiTaRSiGtf2h2 mouse
PROiPR:Q9JIB4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021639 Expressed in 269 organ(s), highest expression level in embryonic stem cell
CleanExiMM_GTF2H2
ExpressionAtlasiQ9JIB4 baseline and differential
GenevisibleiQ9JIB4 MM

Family and domain databases

CDDicd01453 vWA_transcription_factor_IIH_t, 1 hit
Gene3Di3.30.40.10, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR007198 Ssl1-like
IPR004595 TFIIH_C1-like_dom
IPR012170 TFIIH_SSL1/p44
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
IPR013087 Znf_C2H2_type
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR12695 PTHR12695, 1 hit
PfamiView protein in Pfam
PF07975 C1_4, 1 hit
PF04056 Ssl1, 1 hit
PIRSFiPIRSF015919 TFIIH_SSL1, 1 hit
SMARTiView protein in SMART
SM01047 C1_4, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
TIGRFAMsiTIGR00622 ssl1, 1 hit
PROSITEiView protein in PROSITE
PS50234 VWFA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTF2H2_MOUSE
AccessioniPrimary (citable) accession number: Q9JIB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 1, 2000
Last modified: September 12, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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