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Protein

Sphingosine kinase 2

Gene

Sphk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.By similarity

Catalytic activityi

ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei212Proton donor/acceptorBy similarity1
Binding sitei217ATPPROSITE-ProRule annotation1
Binding sitei309SubstrateBy similarity1
Binding sitei316ATPPROSITE-ProRule annotation1
Binding sitei322ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi153 – 155ATPPROSITE-ProRule annotation3
Nucleotide bindingi185 – 189ATPPROSITE-ProRule annotation5
Nucleotide bindingi242 – 244ATPPROSITE-ProRule annotation3
Nucleotide bindingi586 – 588ATPPROSITE-ProRule annotation3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • D-erythro-sphingosine kinase activity Source: MGI
  • NAD+ kinase activity Source: InterPro
  • sphinganine kinase activity Source: UniProtKB
  • sphingosine-1-phosphate receptor activity Source: MGI

GO - Biological processi

  • blood vessel development Source: MGI
  • brain development Source: MGI
  • cell proliferation Source: UniProtKB
  • female pregnancy Source: Ensembl
  • negative regulation of apoptotic process Source: UniProtKB
  • positive regulation of cell proliferation Source: MGI
  • sphinganine-1-phosphate biosynthetic process Source: UniProtKB
  • sphingosine biosynthetic process Source: MGI
  • sphingosine metabolic process Source: MGI

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.91 3474
ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis

Chemistry databases

SwissLipidsiSLP:000000113

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine kinase 2 (EC:2.7.1.91By similarity)
Short name:
SK 2
Short name:
SPK 2
Gene namesi
Name:Sphk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1861380 Sphk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075305

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001813591 – 617Sphingosine kinase 2Add BLAST617

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei358PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei377PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JIA7
MaxQBiQ9JIA7
PaxDbiQ9JIA7
PRIDEiQ9JIA7

PTM databases

iPTMnetiQ9JIA7
PhosphoSitePlusiQ9JIA7
SwissPalmiQ9JIA7

Expressioni

Gene expression databases

BgeeiENSMUSG00000057342
CleanExiMM_SPHK2
ExpressionAtlasiQ9JIA7 baseline and differential
GenevisibleiQ9JIA7 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FynP396882EBI-985434,EBI-524514

Protein-protein interaction databases

IntActiQ9JIA7, 6 interactors
MINTiQ9JIA7
STRINGi10090.ENSMUSP00000072615

Chemistry databases

BindingDBiQ9JIA7

Structurei

3D structure databases

ProteinModelPortaliQ9JIA7
SMRiQ9JIA7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 290DAGKcPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 140Required for binding to sulfatide and phosphoinositides and for membrane localizationBy similarityAdd BLAST140
Regioni210 – 213Substrate bindingBy similarity4

Phylogenomic databases

eggNOGiKOG1116 Eukaryota
COG1597 LUCA
GeneTreeiENSGT00690000101761
HOGENOMiHOG000111460
HOVERGENiHBG054796
InParanoidiQ9JIA7
KOiK04718
OMAiLFHEIVN
OrthoDBiEOG091G06SM
PhylomeDBiQ9JIA7
TreeFamiTF354296

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JIA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPPLLPVA ASTPILHGEF GSYPANGPRF ALTLTTQALH IQRLRPKPEA
60 70 80 90 100
RPRDGLVSLD EVSGCGTLQS RSPEDTAAYF CIYTYPRGRR GGRRRATRTF
110 120 130 140 150
RADGATTYEE NRAEAQRWAT ALTCLLRGVP LSGDQEITPE LLPRKPRLLI
160 170 180 190 200
LVNPFGGRGL AWQRCMDHVV PMISEAGLSF NLIQTERQNH ARELVQGLSL
210 220 230 240 250
SEWEGIVTVS GDGLLYEVLN GLLDRPDWED AVRMPIGVLP CGSGNALAGA
260 270 280 290 300
VNHHGGFEQV VGVDLLLNCS LLLCRGGSHP LDLLSVTLAS GSRCFSFLSV
310 320 330 340 350
AWGFLSDVDI HSERFRALGS ARFTLGAVLG LASLHTYRGR LSYLPATTEP
360 370 380 390 400
ALPIPGHSLP RAKSELVLAP APAPAATHSP LHRSVSDLPL PLPQPALVSP
410 420 430 440 450
GSPEPLPDLS LNGGGPELTG DWGGAGDAPL SPDPLLPSSP NALKTAQLSP
460 470 480 490 500
IAEGPPEMPA SSGFLPPTHS APEASTWGPV DHLLPPLGSP LPQDWVTIEG
510 520 530 540 550
EFVLMLGILP SHLCADLMAA PHARFDDGVV HLCWVRSGIS RAALLRILLA
560 570 580 590 600
MEHGNHFSLG CPHLGYAAAR AFRLEPLTPR GLLTVDGELV EYGPIQAQVH
610
PGLATLLTGP AGQKPQA
Length:617
Mass (Da):65,618
Last modified:March 27, 2002 - v2
Checksum:i40EE2C2C288BE26A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252N → S in AAF74125 (PubMed:10751414).Curated1
Sequence conflicti510P → T in AAF74125 (PubMed:10751414).Curated1
Sequence conflicti548L → F in AAF74125 (PubMed:10751414).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245448 mRNA Translation: AAF74125.1
AF415214 mRNA Translation: AAL07500.1
AK004951 mRNA Translation: BAB23694.1
BC006941 mRNA Translation: AAH06941.1
BC053737 mRNA Translation: AAH53737.1
CCDSiCCDS21261.1
RefSeqiNP_001166032.1, NM_001172561.1
NP_064395.2, NM_020011.5
NP_975009.1, NM_203280.3
XP_006541075.1, XM_006541012.3
XP_006541076.1, XM_006541013.3
UniGeneiMm.24222
Mm.378235

Genome annotation databases

EnsembliENSMUST00000072836; ENSMUSP00000072615; ENSMUSG00000057342
ENSMUST00000107737; ENSMUSP00000103366; ENSMUSG00000057342
ENSMUST00000210060; ENSMUSP00000147391; ENSMUSG00000057342
GeneIDi56632
KEGGimmu:56632
UCSCiuc009gwt.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSPHK2_MOUSE
AccessioniPrimary (citable) accession number: Q9JIA7
Secondary accession number(s): Q91VA9, Q9DBH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: March 27, 2002
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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