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UniProtKB - Q9JI66 (S4A4_RAT)

Entry version 139 (25 May 2022)
Sequence version 1 (01 Oct 2000)
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Protein

Electrogenic sodium bicarbonate cotransporter 1

Gene

Slc4a4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Electrogenic sodium/bicarbonate cotransporter with a Na+:HCO3- stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.

By similarity3 Publications

May have a higher activity than isoform 1.

By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by stilbene derivatives and regulated by cyclic AMP.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processIon transport, Sodium transport, Symport, Transport
LigandSodium

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-RNO-425381, Bicarbonate transporters

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.31.2.2, the anion exchanger (ae) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Electrogenic sodium bicarbonate cotransporter 1
Short name:
Sodium bicarbonate cotransporter
Alternative name(s):
NBC-like protein
Na(+)/HCO3(-) cotransporter
Solute carrier family 4 member 4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Slc4a4
Synonyms:Nbc, Nbc1, Nbce1, Rnbc1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 14

Organism-specific databases

Rat genome database

More...RGDi		68936, Slc4a4

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 466CytoplasmicBy similarityAdd BLAST466
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei467 – 491Helical; Name=1By similarityAdd BLAST25
Topological domaini492 – 501ExtracellularBy similarity10
Transmembranei502 – 520Helical; Name=2By similarityAdd BLAST19
Topological domaini521CytoplasmicBy similarity1
Transmembranei522 – 542Discontinuously helical; Name=3By similarityAdd BLAST21
Topological domaini543 – 550ExtracellularBy similarity8
Transmembranei551 – 571Helical; Name=4By similarityAdd BLAST21
Topological domaini572 – 585CytoplasmicBy similarityAdd BLAST14
Transmembranei586 – 609Helical; Name=5By similarityAdd BLAST24
Topological domaini610 – 692ExtracellularBy similarityAdd BLAST83
Transmembranei693 – 710Helical; Name=6By similarityAdd BLAST18
Topological domaini711 – 725CytoplasmicBy similarityAdd BLAST15
Transmembranei726 – 745Helical; Name=7By similarityAdd BLAST20
Topological domaini746 – 779ExtracellularBy similarityAdd BLAST34
Transmembranei780 – 807Helical; Name=8By similarityAdd BLAST28
Topological domaini808 – 819CytoplasmicBy similarityAdd BLAST12
Transmembranei820 – 836Helical; Name=9By similarityAdd BLAST17
Topological domaini837ExtracellularBy similarity1
Transmembranei838 – 855Discontinuously helical; Name=10By similarityAdd BLAST18
Topological domaini856 – 877CytoplasmicBy similarityAdd BLAST22
Transmembranei878 – 894Helical; Name=11By similarityAdd BLAST17
Topological domaini895 – 901ExtracellularBy similarity7
Transmembranei902 – 918Helical; Name=12By similarityAdd BLAST17
Topological domaini919 – 960CytoplasmicBy similarityAdd BLAST42
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a region that is buried within a membrane, but does not cross it.<p><a href='/help/intramem' target='_top'>More...</a></p>Intramembranei961 – 986Discontinuously helicalBy similarityAdd BLAST26
Topological domaini987 – 1079CytoplasmicBy similarityAdd BLAST93

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi243N → Q: No effect on N-glycosylation. 1 Publication1
Mutagenesisi252N → Q: No effect on N-glycosylation. 1 Publication1
Mutagenesisi541N → Q: No effect on N-glycosylation. 1 Publication1
Mutagenesisi636N → Q: No effect on N-glycosylation. Complete loss of N-glycosylation without effect on transporter basic function; when associated with Q-641 and Q-661. 1 Publication1
Mutagenesisi641N → Q: Reduces the extent of N-glycosylation. Complete loss of N-glycosylation without effect on transporter basic function; when associated with Q-636 and Q-661. 1 Publication1
Mutagenesisi661N → Q: Reduces the extent of N-glycosylation. Complete loss of N-glycosylation without effect on transporter basic function; when associated with Q-636 and Q-641. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000792311 – 1079Electrogenic sodium bicarbonate cotransporter 1Add BLAST1079

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei30PhosphotyrosineBy similarity1
Modified residuei61PhosphoserineBy similarity1
Modified residuei65PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei223PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1
Modified residuei233PhosphoserineBy similarity1
Modified residuei245PhosphoserineCombined sources1
Modified residuei249PhosphothreonineCombined sources1
Modified residuei254PhosphothreonineCombined sources1
Modified residuei256PhosphoserineBy similarity1
Modified residuei257PhosphoserineCombined sources1
Modified residuei262PhosphoserineCombined sources1
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi627 ↔ 629By similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi641N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi661N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi674 ↔ 686By similarity
Modified residuei1026Phosphoserine; by PKABy similarity1
Modified residuei1029PhosphoserineCombined sources1
Modified residuei1034PhosphoserineCombined sources1
Modified residuei1044PhosphoserineBy similarity1
Modified residuei1069PhosphoserineCombined sources1
Isoform 2 (identifier: Q9JI66-2)
Modified residuei2PhosphoserineCombined sources1
Isoform 3 (identifier: Q9JI66-3)
Modified residuei1026PhosphoserineCombined sources1
Modified residuei1029PhosphoserineCombined sources1
Modified residuei1060PhosphoserineCombined sources1
Modified residuei1064PhosphoserineCombined sources1
Modified residuei1078PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na+:HCO3- stoichiometry of the transporter from 3:1 to 2:1. Phosphorylated in presence of STK39 and dephosphorylated in presence of PP1 phosphatase; phosphorylation seems to inhibit SLC4A4 activity.By similarity
N-glycosylation is not necessary for the transporter basic functions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei636Not glycosylated1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q9JI66

PRoteomics IDEntifications database

More...PRIDEi		Q9JI66

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q9JI66, 2 sites

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q9JI66

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q9JI66

SwissPalm database of S-palmitoylation events

More...SwissPalmi		Q9JI66

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Specifically expressed in kidney and to a lower extent in liver, lung, spleen, brain, skeletal muscle and heart. In kidney, expressed in proximal tubules at the corticomedullary junction. Isoform 2 is specifically expressed in kidney. Isoform 1 is expressed in kidney and pancreas while isoform 3 is specifically expressed in brain (at protein level). In brain, isoform 1 is expressed in astrocytes while isoform 3 is expressed in neurons (at protein level). In the eye, isoform 1 is expressed in cornea, conjunctiva, lens epithelium, ciliary bodies and retina while isoform 2 is detected only in the conjunctiva.7 Publications

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expression is first detected at E17 in spinal cord and starts in forebrain at birth. Higher expression in brain is detected at postnatal day 15 and persists throughout adulthood.1 Publication

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Down-regulated after cadmium-intoxication and sodium or bicarbonate loading (at protein level). Down-regulated by HCO3[-] loading.3 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSRNOG00000003134, Expressed in adult mammalian kidney and 20 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q9JI66, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q9JI66, RN

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

Interacts with CA2/carbonic anhydrase 2 and CA4/carbonic anhydrase 4 which may regulate transporter activity. Isoform 1 but not isoform 2 interacts with AHCYL1 (via PEST domain when phosphorylated); the interaction increases SLC4A4 isoform 1 activity.

Interacts with AHCYL2.

By similarity

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		249983, 4 interactors

Protein interaction database and analysis system

More...IntActi		Q9JI66, 2 interactors

Molecular INTeraction database

More...MINTi		Q9JI66

STRING: functional protein association networks

More...STRINGi		10116.ENSRNOP00000004391

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q9JI66

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q9JI66

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 62Required for interaction with AHCYL1By similarityAdd BLAST62
Regioni39 – 78DisorderedSequence analysisAdd BLAST40
Regioni235 – 266DisorderedSequence analysisAdd BLAST32
Regioni748 – 779Interaction with CA4By similarityAdd BLAST32
Regioni1002 – 1004CA2-bindingBy similarity3
Regioni1012 – 1079DisorderedSequence analysisAdd BLAST68
Regioni1030 – 1033CA2-bindingBy similarity4
Regioni1057 – 1059Required for basolateral targetingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi39 – 53Basic residuesSequence analysisAdd BLAST15
Compositional biasi54 – 73Basic and acidic residuesSequence analysisAdd BLAST20
Compositional biasi235 – 260Polar residuesSequence analysisAdd BLAST26
Compositional biasi1012 – 1039Basic and acidic residuesSequence analysisAdd BLAST28
Compositional biasi1060 – 1079Basic and acidic residuesSequence analysisAdd BLAST20

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1172, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000156290

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_002289_5_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q9JI66

Database of Orthologous Groups

More...OrthoDBi		265068at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q9JI66

TreeFam database of animal gene trees

More...TreeFami		TF313630

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.930.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR013769, Band3_cytoplasmic_dom
IPR011531, HCO3_transpt-like_TM_dom
IPR003020, HCO3_transpt_euk
IPR003024, Na/HCO3_transpt
IPR016152, PTrfase/Anion_transptr

The PANTHER Classification System

More...PANTHERi		PTHR11453, PTHR11453, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF07565, Band_3_cyto, 1 hit
PF00955, HCO3_cotransp, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR01231, HCO3TRNSPORT
PR01232, NAHCO3TRSPRT

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF55804, SSF55804, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00834, ae, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9JI66-1) [UniParc]FASTAAdd to basket
Also known as: bNBC1, NBCe1-B, pNBC1, pNBC-1, rb1NBC

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDEAVLDRG ASFLKHVCDE EEVEGHHTIY IGVHVPKSYR RRRRHKRKAG 
        60         70         80         90        100
HKEKKEKERI SENYSDKSDV ENADESSSSI LKPLISPAAE RIRFILGEED 
       110        120        130        140        150
DSPAPPQLFT ELDELLAVDG QEMEWKETAR WIKFEEKVEQ GGERWSKPHV 
       160        170        180        190        200
ATLSLHSLFE LRTCMEKGSI MLDREASSLP QLVEMIADHQ IETGLLKPDL 
       210        220        230        240        250
KDKVTYTLLR KHRHQTKKSN LRSLADIGKT VSSASRMFSN PDNGSPAMTH 
       260        270        280        290        300
RNLTSSSLND ISDKPEKDQL KNKFMKKLPR DAEASNVLVG EVDFLDTPFI 
       310        320        330        340        350
AFVRLQQAVM LGALTEVPVP TRFLFILLGP KGKAKSYHEI GRAIATLMSD 
       360        370        380        390        400
EVFHDIAYKA KDRHDLIAGI DEFLDEVIVL PPGEWDPAIR IEPPKSLPSS 
       410        420        430        440        450
DKRKNMYSGG ENVQMNGDTP HDGGHGGGGH GDCEELQRTG RFCGGLIKDI 
       460        470        480        490        500
KRKAPFFASD FYDALNIQAL SAILFIYLAT VTNAITFGGL LGDATDNMQG 
       510        520        530        540        550
VLESFLGTAV SGAIFCLFAG QPLTILSSTG PVLVFERLLF NFSKDHSFDY 
       560        570        580        590        600
LEFRLWIGLW SAFMCLILVA TDASFLVQYF TRFTEEGFSS LISFIFIYDA 
       610        620        630        640        650
FKKMIKLADY YPINSDFRVG YNTHFSCACL PPDPVNLSVS NDTTLAPEDL 
       660        670        680        690        700
PTVSSTDMYH NATFDWAYLS KKECVKFGGK LVGNNCDFVP DITLMSFILF 
       710        720        730        740        750
LGTYTSSMAM KKFKTSRYFP TTARKLISDF AIILSILIFC VIDALVGVDT 
       760        770        780        790        800
PKLIVPSEFK PTSPHRGWFV PPFGGNPWWV CLAAAIPALL VTILIFMDQQ 
       810        820        830        840        850
ITAVIVNRKE HKLKKGAGYH LDLFWVAILM VVCSFMALPW YVAATVISIA 
       860        870        880        890        900
HIDSLKMETE TSAPGEQPKF LGVREQRVTG TLVFILTGLS VFMAPILKFI 
       910        920        930        940        950
PMPVLYGVFL YMGVASLNGV QFMDRLKLLL MPLKHQPDFI YLRHVPLRRV 
       960        970        980        990       1000
HLFTSLQVLC LALLWILKST VAAIIFPVMI LALVAVRKGM DYLFSQHDLS 
      1010       1020       1030       1040       1050
FLDDVIPEKD KKKKEDEKKK KKKKGSLDSD NDDSDCPYSE KVPSIKIPMD 
      1060       1070 
ITEQQPFLSD NKPLDRERSS TFLERHTSC
Length:1,079
Mass (Da):121,343
Last modified:October 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF5A187AFE41BA5B4
GO
Isoform 2 (identifier: Q9JI66-2) [UniParc]FASTAAdd to basket
Also known as: kNBC1, kNBC-1, NBCe1-A, rkNBC, rkNBC1

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     45-85: HKRKAGHKEK...SSSSILKPLI → MSTENVEGKP...FNHSIFTSAV

Note: Mutagenesis of Asn-33 into Gln has no effect on N-glycosylation.Curated
Show »
Length:1,035
Mass (Da):116,042
Checksum:i5BAB6B0152DE31FD
GO
Isoform 3 (identifier: Q9JI66-3) [UniParc]FASTAAdd to basket
Also known as: NBCe1-C, rb2NBC

The sequence of this isoform differs from the canonical sequence as follows:
     1034-1079: SDCPYSEKVP...STFLERHTSC → EKDPQHSLNA...WRNKGTETTL

Show »
Length:1,094
Mass (Da):122,962
Checksum:i1DD46D615F650311
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0H2UHB7A0A0H2UHB7_RAT
Anion exchange protein
Slc4a4
1,104Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A8I6ABC3A0A8I6ABC3_RAT
Electrogenic sodium bicarbonate cot...
Slc4a4
1,084Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A8I6AFE1A0A8I6AFE1_RAT
Electrogenic sodium bicarbonate cot...
Slc4a4
1,099Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti284 – 285AS → VP in AAB83997 (PubMed:9841505).Curated2
Sequence conflicti284 – 285AS → VP in AAF21040 (PubMed:10648705).Curated2
Sequence conflicti479A → G in AAB83997 (PubMed:9841505).Curated1
Sequence conflicti479A → G in AAF21040 (PubMed:10648705).Curated1
Sequence conflicti640S → Y in AAB83997 (PubMed:9841505).Curated1
Sequence conflicti655S → F in AAB83997 (PubMed:9841505).Curated1
Sequence conflicti803A → P in AAB83997 (PubMed:9841505).Curated1
Sequence conflicti803A → P in AAF21040 (PubMed:10648705).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0167171 – 44Missing in isoform 2. 2 PublicationsAdd BLAST44
Alternative sequenceiVSP_01671845 – 85HKRKA…LKPLI → MSTENVEGKPNNLGERGRAR SSTFLRVFQPMFNHSIFTSA V in isoform 2. 2 PublicationsAdd BLAST41
Alternative sequenceiVSP_0167191034 – 1079SDCPY…RHTSC → EKDPQHSLNATHHADKIPFL ESLGLPSPPRSPVKVVPQIR IELESEDNDYLWRNKGTETT L in isoform 3. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF004017 mRNA Translation: AAC40034.1
AF027362 mRNA Translation: AAB83997.1
AF124441 mRNA Translation: AAF87312.1
AF254802 mRNA Translation: AAF87553.1
AF210250 mRNA Translation: AAF21040.1

Protein sequence database of the Protein Information Resource

More...PIRi		T13962
T14110

NCBI Reference Sequences

More...RefSeqi		NP_445876.1, NM_053424.1 [Q9JI66-1]
XP_006250853.1, XM_006250791.3 [Q9JI66-3]

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...GeneIDi		84484

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		rno:84484

UCSC genome browser

More...UCSCi		RGD:68936, rat [Q9JI66-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004017 mRNA Translation: AAC40034.1
AF027362 mRNA Translation: AAB83997.1
AF124441 mRNA Translation: AAF87312.1
AF254802 mRNA Translation: AAF87553.1
AF210250 mRNA Translation: AAF21040.1
PIRiT13962
T14110
RefSeqiNP_445876.1, NM_053424.1 [Q9JI66-1]
XP_006250853.1, XM_006250791.3 [Q9JI66-3]

3D structure databases

AlphaFoldDBiQ9JI66
SMRiQ9JI66
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi249983, 4 interactors
IntActiQ9JI66, 2 interactors
MINTiQ9JI66
STRINGi10116.ENSRNOP00000004391

Protein family/group databases

TCDBi2.A.31.2.2, the anion exchanger (ae) family

PTM databases

GlyGeniQ9JI66, 2 sites
iPTMnetiQ9JI66
PhosphoSitePlusiQ9JI66
SwissPalmiQ9JI66

Proteomic databases

PaxDbiQ9JI66
PRIDEiQ9JI66

Genome annotation databases

GeneIDi84484
KEGGirno:84484
UCSCiRGD:68936, rat [Q9JI66-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		8671
RGDi68936, Slc4a4

Phylogenomic databases

eggNOGiKOG1172, Eukaryota
GeneTreeiENSGT00940000156290
HOGENOMiCLU_002289_5_0_1
InParanoidiQ9JI66
OrthoDBi265068at2759
PhylomeDBiQ9JI66
TreeFamiTF313630

Enzyme and pathway databases

ReactomeiR-RNO-425381, Bicarbonate transporters

Miscellaneous databases

Protein Ontology

More...PROi		PR:Q9JI66

Gene expression databases

BgeeiENSRNOG00000003134, Expressed in adult mammalian kidney and 20 other tissues
ExpressionAtlasiQ9JI66, baseline and differential
GenevisibleiQ9JI66, RN

Family and domain databases

Gene3Di3.40.930.10, 1 hit
InterProiView protein in InterPro
IPR013769, Band3_cytoplasmic_dom
IPR011531, HCO3_transpt-like_TM_dom
IPR003020, HCO3_transpt_euk
IPR003024, Na/HCO3_transpt
IPR016152, PTrfase/Anion_transptr
PANTHERiPTHR11453, PTHR11453, 1 hit
PfamiView protein in Pfam
PF07565, Band_3_cyto, 1 hit
PF00955, HCO3_cotransp, 1 hit
PRINTSiPR01231, HCO3TRNSPORT
PR01232, NAHCO3TRSPRT
SUPFAMiSSF55804, SSF55804, 1 hit
TIGRFAMsiTIGR00834, ae, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiS4A4_RAT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9JI66
Secondary accession number(s): O35422
, O54815, Q9JJ32, Q9QXH6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: May 25, 2022
This is version 139 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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