UniProtKB - Q9JI61 (LRAT_RAT)
Protein
Lecithin retinol acyltransferase
Gene
Lrat
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters (PubMed:3410848). Retinyl esters are storage forms of vitamin A (By similarity). LRAT plays a critical role in vision (By similarity). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (By similarity). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology (By similarity).By similarity2 Publications
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + all-trans-retinol--[retinol-binding protein] = a 2-acyl-sn-glycero-3-phosphocholine + an all-trans-retinyl ester + apo--[retinol-binding protein]By similarityEC:2.3.1.135By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + all-trans-retinol = all-trans-retinyl hexadecanoate + hexadecanoyl-sn-glycero-3-phosphocholine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-diheptanoyl-sn-glycero-3-phosphocholine + all-trans-retinol--[retinol-binding protein] = 2-heptanoyl-sn-glycero-3-phosphocholine + all-trans-retinyl heptanoate + apo--[retinol-binding protein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-dioctanoyl-sn-glycero-3-phosphocholine + all-trans-retinol--[retinol-binding protein] = 2-octanoyl-sn-glycero-3-phosphocholine + all-trans-retinyl octanoate + apo--[retinol-binding protein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + all-trans-retinol--[retinol-binding protein] = 2-hexadecanoyl-sn-glycero-3-phosphocholine + all-trans-retinyl hexadecanoate + apo--[retinol-binding protein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-didodecanoyl-sn-glycero-3-phosphocholine + all-trans-retinol--[retinol-binding protein] = 2-dodecanoyl-sn-glycero-3-phosphocholine + all-trans-retinyl dodecanoate + apo--[retinol-binding protein]By similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Inhibited by all-trans-retinyl alpha-bromoacetate and N-boc-L-biocytinyl-11-aminoundecane chloro-methyl ketone (BACMK).By similarity
: retinol metabolism Pathwayi
This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.View all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 60 | PROSITE-ProRule annotation | 1 | |
Active sitei | 72 | PROSITE-ProRule annotation | 1 | |
Active sitei | 161 | Acyl-thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- lecithin:11-cis retinol acyltransferase activity Source: UniProtKB-EC
- O-acyltransferase activity Source: RGD
- phosphatidylcholine-retinol O-acyltransferase activity Source: RGD
- retinoic acid binding Source: RGD
- retinol binding Source: RGD
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: RGD
- positive regulation of lipid transport Source: RGD
- response to bacterium Source: RGD
- response to retinoic acid Source: RGD
- response to vitamin A Source: RGD
- retinol metabolic process Source: RGD
- visual perception Source: UniProtKB-KW
- vitamin A metabolic process Source: RGD
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Sensory transduction, Vision |
Enzyme and pathway databases
BRENDAi | 2.3.1.135, 5301 |
Reactomei | R-RNO-2453902, The canonical retinoid cycle in rods (twilight vision) R-RNO-975634, Retinoid metabolism and transport |
UniPathwayi | UPA00912 |
Chemistry databases
SwissLipidsi | SLP:000001908 |
Names & Taxonomyi
Protein namesi | Recommended name: Lecithin retinol acyltransferase (EC:2.3.1.135)Alternative name(s): Phosphatidylcholine--retinol O-acyltransferase |
Gene namesi | Name:Lrat |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 68362, Lrat |
Subcellular locationi
Endosome
- multivesicular body 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity
- Rough endoplasmic reticulum 1 Publication
Other locations
- perinuclear region 1 Publication
Note: Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells.
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
- rough endoplasmic reticulum Source: UniProtKB
Endosome
- multivesicular body Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: RGD
- perinuclear region of cytoplasm Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 194 | CytoplasmicBy similarityAdd BLAST | 194 | |
Transmembranei | 195 – 215 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 216 – 231 | LumenalBy similarityAdd BLAST | 16 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000152480 | 1 – 231 | Lecithin retinol acyltransferaseAdd BLAST | 231 |
Proteomic databases
PaxDbi | Q9JI61 |
Expressioni
Tissue specificityi
Hepatic stellate cells and endothelial cells (at protein level). Highly expressed in adrenal gland, small intestine, testis and eye. Lower levels of expression are observed in liver, heart, lung, skin, mammary tissue and skeletal muscle.2 Publications
Inductioni
LRAT activity is up-regulated by dietary vitamin A. Under conditions of vitamin A depletion, LRAT expression in the liver is induced by retinoic acid.2 Publications
Gene expression databases
Bgeei | ENSRNOG00000025608, Expressed in liver and 17 other tissues |
ExpressionAtlasi | Q9JI61, baseline and differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 177 | LRATPROSITE-ProRule annotationAdd BLAST | 128 |
Sequence similaritiesi
Belongs to the H-rev107 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QWSA, Eukaryota |
GeneTreei | ENSGT00510000047351 |
HOGENOMi | CLU_105262_0_0_1 |
InParanoidi | Q9JI61 |
OMAi | FCLWMAG |
OrthoDBi | 1602481at2759 |
PhylomeDBi | Q9JI61 |
TreeFami | TF330836 |
Family and domain databases
InterProi | View protein in InterPro IPR042288, LRAT IPR007053, LRAT_dom |
PANTHERi | PTHR46678, PTHR46678, 1 hit |
Pfami | View protein in Pfam PF04970, LRAT, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9JI61-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNSMLEAAS LLLEKLLLIS NFKIFSVCAP GGGTGKKHPY EINSFLRGDV
60 70 80 90 100
LEVSRTHFTH YGIYLGDNRV AHLMPDILLA LTSDKERTQK VVSNKRLLPG
110 120 130 140 150
VICKVASIRV DTVEDFAYGA DILVNHLDET LKKKSLLNEE VARRAEQQLG
160 170 180 190 200
LTPYSLLWNN CEHFVTYCRY GSPISPQAEK FHETVKILIR DQRSCLASAV
210 220 230
LGLVSIIYTG LASYMTLPAV CIPFCLWMMS G
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K641 | A0A0G2K641_RAT | Lecithin retinol acyltransferase | Lrat | 52 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF255060 mRNA Translation: AAF97786.1 |
RefSeqi | NP_071616.1, NM_022280.2 |
Genome annotation databases
Ensembli | ENSRNOT00000035411; ENSRNOP00000035053; ENSRNOG00000025608 |
GeneIDi | 64047 |
KEGGi | rno:64047 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF255060 mRNA Translation: AAF97786.1 |
RefSeqi | NP_071616.1, NM_022280.2 |
3D structure databases
SMRi | Q9JI61 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000035053 |
Chemistry databases
SwissLipidsi | SLP:000001908 |
Proteomic databases
PaxDbi | Q9JI61 |
Genome annotation databases
Ensembli | ENSRNOT00000035411; ENSRNOP00000035053; ENSRNOG00000025608 |
GeneIDi | 64047 |
KEGGi | rno:64047 |
Organism-specific databases
CTDi | 9227 |
RGDi | 68362, Lrat |
Phylogenomic databases
eggNOGi | ENOG502QWSA, Eukaryota |
GeneTreei | ENSGT00510000047351 |
HOGENOMi | CLU_105262_0_0_1 |
InParanoidi | Q9JI61 |
OMAi | FCLWMAG |
OrthoDBi | 1602481at2759 |
PhylomeDBi | Q9JI61 |
TreeFami | TF330836 |
Enzyme and pathway databases
UniPathwayi | UPA00912 |
BRENDAi | 2.3.1.135, 5301 |
Reactomei | R-RNO-2453902, The canonical retinoid cycle in rods (twilight vision) R-RNO-975634, Retinoid metabolism and transport |
Miscellaneous databases
PROi | PR:Q9JI61 |
Gene expression databases
Bgeei | ENSRNOG00000025608, Expressed in liver and 17 other tissues |
ExpressionAtlasi | Q9JI61, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR042288, LRAT IPR007053, LRAT_dom |
PANTHERi | PTHR46678, PTHR46678, 1 hit |
Pfami | View protein in Pfam PF04970, LRAT, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LRAT_RAT | |
Accessioni | Q9JI61Primary (citable) accession number: Q9JI61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families