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Protein

Diphosphoinositol polyphosphate phosphohydrolase 1

Gene

Nudt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. InsP6 (inositol hexakisphophate) is not a substrate. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate (By similarity). Acts as a negative regulator of the ERK1/2 pathway.By similarity

Catalytic activityi

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by fluoride and InsP6.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10SubstrateBy similarity1
Metal bindingi50Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi66Magnesium 2By similarity1
Metal bindingi66Magnesium 3By similarity1
Active sitei69Proton acceptorBy similarity1
Metal bindingi70Magnesium 1By similarity1
Binding sitei115SubstrateBy similarity1
Binding sitei133SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1855167 Synthesis of pyrophosphates in the cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Diphosphoinositol polyphosphate phosphohydrolase 1Curated (EC:3.6.1.52By similarity)
Short name:
DIPP-1
Short name:
muDIPP1
Alternative name(s):
Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 1 (EC:3.6.1.-)
Nucleoside diphosphate-linked moiety X motif 3
Short name:
Nudix motif 3
Gene namesi
Name:Nudt3Imported
Synonyms:Dipp, Dipp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1928484 Nudt3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70E → Q: Loss of enzyme activity, but retains ability to regulate the ERK1/2 pathway. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000570561 – 168Diphosphoinositol polyphosphate phosphohydrolase 1Add BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9JI46
MaxQBiQ9JI46
PaxDbiQ9JI46
PRIDEiQ9JI46

PTM databases

iPTMnetiQ9JI46
PhosphoSitePlusiQ9JI46

Expressioni

Tissue specificityi

Present in heart, lung, liver and spleen (at protein level). Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000024213
ExpressionAtlasiQ9JI46 baseline and differential
GenevisibleiQ9JI46 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ9JI46, 1 interactor
MINTiQ9JI46
STRINGi10090.ENSMUSP00000025050

Structurei

3D structure databases

ProteinModelPortaliQ9JI46
SMRiQ9JI46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 144Nudix hydrolasePROSITE-ProRule annotationAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 20Substrate bindingBy similarity3
Regioni39 – 41Substrate bindingBy similarity3
Regioni89 – 91Substrate bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi51 – 72Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. DIPP subfamily.Curated

Phylogenomic databases

eggNOGiKOG2839 Eukaryota
COG0494 LUCA
GeneTreeiENSGT00390000012928
HOGENOMiHOG000237336
HOVERGENiHBG053341
InParanoidiQ9JI46
KOiK07766
OMAiNNGTPVM
OrthoDBiEOG091G0TH6
PhylomeDBiQ9JI46
TreeFamiTF106349

Family and domain databases

InterProiView protein in InterPro
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
SUPFAMiSSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JI46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKLKSNQTR TYDGDGYKKR AACLCFRSES EEEVLLVSSS RHPDRWIVPG
60 70 80 90 100
GGMEPEEEPS VAAVREVCEE AGVKGTLGRL VGIFENQERK HRTYVYVLIV
110 120 130 140 150
TEVLEDWEDS VNIGRKREWF KIEDAIKVLQ CHKPVQASYF ETLRQGYPAN
160
NGTPVVPTTY SSSVSGIR
Length:168
Mass (Da):19,030
Last modified:October 1, 2000 - v1
Checksum:iE543BE5CBE520910
GO

Sequence cautioni

The sequence AAH57331 differs from that shown. Chimeric at the C-terminus.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264064 mRNA Translation: AAF74761.1
CT027568 Genomic DNA No translation available.
BC016534 mRNA Translation: AAH16534.1
BC046805 mRNA Translation: AAH46805.1
BC057331 mRNA Translation: AAH57331.1 Sequence problems.
AK079658 mRNA Translation: BAC37717.1
CCDSiCCDS28566.1
RefSeqiNP_062811.1, NM_019837.2
UniGeneiMm.144699
Mm.32556
Mm.473041

Genome annotation databases

EnsembliENSMUST00000025050; ENSMUSP00000025050; ENSMUSG00000024213
GeneIDi56409
KEGGimmu:56409
UCSCiuc008bpg.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNUDT3_MOUSE
AccessioniPrimary (citable) accession number: Q9JI46
Secondary accession number(s): B2KF68, Q6PG02, Q8BV71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2000
Last modified: July 18, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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