UniProtKB - Q9JHY1 (JAM1_RAT)
Protein
Junctional adhesion molecule A
Gene
F11r
Organism
Rattus norvegicus (Rat)
Status
Functioni
Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration.By similarity
GO - Molecular functioni
- integrin binding Source: RGD
- PDZ domain binding Source: RGD
- protein homodimerization activity Source: RGD
GO - Biological processi
- actomyosin structure organization Source: RGD
- cell adhesion Source: RGD
- cell-cell adhesion Source: RGD
- cell differentiation Source: RGD
- cellular response to mechanical stimulus Source: RGD
- epithelial cell differentiation Source: RGD
- establishment of endothelial intestinal barrier Source: RGD
- intestinal absorption Source: RGD
- leukocyte cell-cell adhesion Source: RGD
- memory T cell extravasation Source: RGD
- negative regulation of GTPase activity Source: RGD
- negative regulation of stress fiber assembly Source: RGD
- positive regulation of blood pressure Source: RGD
- positive regulation of establishment of endothelial barrier Source: RGD
- positive regulation of GTPase activity Source: RGD
- positive regulation of platelet aggregation Source: RGD
- positive regulation of Rho protein signal transduction Source: RGD
- protein localization to bicellular tight junction Source: RGD
- protein localization to plasma membrane Source: RGD
- regulation of actin cytoskeleton organization Source: RGD
- regulation of actin cytoskeleton reorganization Source: RGD
- regulation of bicellular tight junction assembly Source: RGD
- regulation of cell shape Source: RGD
- regulation of cytokine production Source: RGD
- regulation of cytoskeleton organization Source: RGD
- regulation of membrane permeability Source: RGD
- response to radiation Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-202733, Cell surface interactions at the vascular wall R-RNO-216083, Integrin cell surface interactions R-RNO-2173791, TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-RNO-420029, Tight junction interactions |
Names & Taxonomyi
Protein namesi | Recommended name: Junctional adhesion molecule AShort name: JAM-A Alternative name(s): Junctional adhesion molecule 1 Short name: JAM-1 CD_antigen: CD321 |
Gene namesi | Name:F11r Synonyms:Jam1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621842, F11r |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein By similarity
Other locations
- tight junction By similarity
Note: Localized at tight junctions of both epithelial and endothelial cells.By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- bicellular tight junction Source: RGD
- cell-cell junction Source: RGD
- cytoplasmic vesicle Source: RGD
- integral component of membrane Source: UniProtKB-KW
- protein-containing complex Source: RGD
- slit diaphragm Source: RGD
- tight junction Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 27 – 238 | ExtracellularSequence analysisAdd BLAST | 212 | |
Transmembranei | 239 – 259 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 260 – 300 | CytoplasmicSequence analysisAdd BLAST | 41 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000015068 | 27 – 300 | Junctional adhesion molecule AAdd BLAST | 274 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 ↔ 108 | PROSITE-ProRule annotation | ||
Disulfide bondi | 152 ↔ 212 | PROSITE-ProRule annotation | ||
Glycosylationi | 185 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 282 | PhosphoserineBy similarity | 1 | |
Modified residuei | 285 | PhosphoserineCombined sources | 1 | |
Modified residuei | 288 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q9JHY1 |
PRIDEi | Q9JHY1 |
PTM databases
GlyGeni | Q9JHY1, 1 site |
iPTMneti | Q9JHY1 |
PhosphoSitePlusi | Q9JHY1 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000004414, Expressed in lung and 21 other tissues |
Genevisiblei | Q9JHY1, RN |
Interactioni
Subunit structurei
Interacts with the ninth PDZ domain of MPDZ.
Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction.
Interacts with ITGAL (via I-domain).
By similarityGO - Molecular functioni
- integrin binding Source: RGD
- PDZ domain binding Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
CORUMi | Q9JHY1 |
IntActi | Q9JHY1, 1 interactor |
MINTi | Q9JHY1 |
STRINGi | 10116.ENSRNOP00000006141 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 122 | Ig-like V-type 1Add BLAST | 95 | |
Domaini | 134 – 228 | Ig-like V-type 2Add BLAST | 95 |
Domaini
The Ig-like V-type 2 domain is necessary and sufficient for interaction with integrin alpha-L/beta-2.By similarity
Sequence similaritiesi
Belongs to the immunoglobulin superfamily.Curated
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QWVN, Eukaryota |
GeneTreei | ENSGT00940000159186 |
HOGENOMi | CLU_067351_0_0_1 |
InParanoidi | Q9JHY1 |
OMAi | YSRGYFE |
OrthoDBi | 1122085at2759 |
PhylomeDBi | Q9JHY1 |
TreeFami | TF331459 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR042456, F11R IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set |
PANTHERi | PTHR45113, PTHR45113, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits SM00406, IGv, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9JHY1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTEGKAGSK LLFLFTSMIL GSLVQGKGSV YSPQTAVQVP ENDSVKLPCI
60 70 80 90 100
YSGFSSPRVE WKFVQGSTTA LVCYNNQITV PYADRVTFSS SGITFSSVTR
110 120 130 140 150
KDNGEYTCMV SEDGGQNYGE VSIHLTVLVP PSKPTVSIPS SVTIGNRAVL
160 170 180 190 200
TCSEHDGSPP SEYSWFKDGV PMLTADAKKT RAFINSSYTI DPKSGDLVFD
210 220 230 240 250
PVSAFDSGEY YCEAQNGYGT AMRSEAVRME AVELNVGGIV AAVLVTLILL
260 270 280 290 300
GLLIFGIWFA YSRGYFERTK KGTAPGKKVI YSQPSARSEG EFKQTSSFLV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF276998 mRNA Translation: AAF78250.1 BC065309 mRNA Translation: AAH65309.1 |
RefSeqi | NP_446248.1, NM_053796.1 |
Genome annotation databases
Ensembli | ENSRNOT00000006141; ENSRNOP00000006141; ENSRNOG00000004414 |
GeneIDi | 116479 |
KEGGi | rno:116479 |
UCSCi | RGD:621842, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF276998 mRNA Translation: AAF78250.1 BC065309 mRNA Translation: AAH65309.1 |
RefSeqi | NP_446248.1, NM_053796.1 |
3D structure databases
SMRi | Q9JHY1 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | Q9JHY1 |
IntActi | Q9JHY1, 1 interactor |
MINTi | Q9JHY1 |
STRINGi | 10116.ENSRNOP00000006141 |
PTM databases
GlyGeni | Q9JHY1, 1 site |
iPTMneti | Q9JHY1 |
PhosphoSitePlusi | Q9JHY1 |
Proteomic databases
PaxDbi | Q9JHY1 |
PRIDEi | Q9JHY1 |
Genome annotation databases
Ensembli | ENSRNOT00000006141; ENSRNOP00000006141; ENSRNOG00000004414 |
GeneIDi | 116479 |
KEGGi | rno:116479 |
UCSCi | RGD:621842, rat |
Organism-specific databases
CTDi | 50848 |
RGDi | 621842, F11r |
Phylogenomic databases
eggNOGi | ENOG502QWVN, Eukaryota |
GeneTreei | ENSGT00940000159186 |
HOGENOMi | CLU_067351_0_0_1 |
InParanoidi | Q9JHY1 |
OMAi | YSRGYFE |
OrthoDBi | 1122085at2759 |
PhylomeDBi | Q9JHY1 |
TreeFami | TF331459 |
Enzyme and pathway databases
Reactomei | R-RNO-202733, Cell surface interactions at the vascular wall R-RNO-216083, Integrin cell surface interactions R-RNO-2173791, TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-RNO-420029, Tight junction interactions |
Miscellaneous databases
PROi | PR:Q9JHY1 |
Gene expression databases
Bgeei | ENSRNOG00000004414, Expressed in lung and 21 other tissues |
Genevisiblei | Q9JHY1, RN |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR042456, F11R IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set |
PANTHERi | PTHR45113, PTHR45113, 1 hit |
Pfami | View protein in Pfam PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits SM00406, IGv, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | JAM1_RAT | |
Accessioni | Q9JHY1Primary (citable) accession number: Q9JHY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families