UniProtKB - Q9JHX4 (CASP8_RAT)
Caspase-8
Casp8
Functioni
Catalytic activityi
- Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala).By similarity EC:3.4.22.61
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 319 | By similarity | 1 | |
Active sitei | 362 | By similarity | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: RGD
- cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: GO_Central
- death effector domain binding Source: GO_Central
- death receptor binding Source: RGD
- identical protein binding Source: Ensembl
- protein-containing complex binding Source: RGD
- scaffold protein binding Source: Ensembl
- tumor necrosis factor receptor binding Source: RGD
- ubiquitin protein ligase binding Source: Ensembl
GO - Biological processi
- activation of cysteine-type endopeptidase activity Source: Ensembl
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
- apoptotic process Source: RGD
- cellular response to mechanical stimulus Source: Ensembl
- cellular response to organic cyclic compound Source: RGD
- execution phase of apoptosis Source: UniProtKB
- extrinsic apoptotic signaling pathway via death domain receptors Source: GO_Central
- macrophage differentiation Source: GO_Central
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
- positive regulation of interleukin-1 beta production Source: Ensembl
- positive regulation of macrophage differentiation Source: Ensembl
- positive regulation of neuron death Source: RGD
- proteolysis Source: RGD
- proteolysis involved in cellular protein catabolic process Source: Ensembl
- response to antibiotic Source: RGD
- response to cobalt ion Source: RGD
- response to cold Source: RGD
- response to estradiol Source: RGD
- response to ethanol Source: RGD
- response to lipopolysaccharide Source: RGD
- response to tumor necrosis factor Source: Ensembl
- TRAIL-activated apoptotic signaling pathway Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Apoptosis |
Enzyme and pathway databases
BRENDAi | 3.4.22.61 5301 |
Reactomei | R-RNO-111465 Apoptotic cleavage of cellular proteins R-RNO-168638 NOD1/2 Signaling Pathway R-RNO-264870 Caspase-mediated cleavage of cytoskeletal proteins R-RNO-3371378 Regulation by c-FLIP R-RNO-5213460 RIPK1-mediated regulated necrosis R-RNO-5218900 CASP8 activity is inhibited R-RNO-5357905 Regulation of TNFR1 signaling R-RNO-5660668 CLEC7A/inflammasome pathway R-RNO-69416 Dimerization of procaspase-8 R-RNO-75108 Activation, myristolyation of BID and translocation to mitochondria R-RNO-75153 Apoptotic execution phase R-RNO-75157 FasL/ CD95L signaling R-RNO-75158 TRAIL signaling |
Protein family/group databases
MEROPSi | C14.009 |
Names & Taxonomyi
Protein namesi | Recommended name: Caspase-81 PublicationCurated (EC:3.4.22.61)Short name: CASP-8 Cleaved into the following 2 chains: |
Gene namesi | Name:Casp8Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620945 Casp8 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: RGD
- ripoptosome Source: UniProtKB
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: RGD
Plasma Membrane
- CD95 death-inducing signaling complex Source: RGD
- death-inducing signaling complex Source: RGD
Other locations
- cell body Source: RGD
- cytoplasm Source: RGD
- membrane raft Source: RGD
- neuron projection Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000432425 | 1 – 218 | By similarityAdd BLAST | 218 | |
ChainiPRO_0000432426 | 219 – 378 | Caspase-8 subunit p18Add BLAST | 160 | |
PropeptideiPRO_0000432427 | 379 – 388 | By similarity | 10 | |
ChainiPRO_0000432428 | 389 – 482 | Caspase-8 subunit p10Add BLAST | 94 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 188 | PhosphoserineBy similarity | 1 | |
Modified residuei | 213 | PhosphoserineBy similarity | 1 | |
Modified residuei | 336 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 390 | Phosphoserine; by CDK1By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, ZymogenProteomic databases
PaxDbi | Q9JHX4 |
PRIDEi | Q9JHX4 |
PTM databases
iPTMneti | Q9JHX4 |
PhosphoSitePlusi | Q9JHX4 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000012331 Expressed in 10 organ(s), highest expression level in colon |
Genevisiblei | Q9JHX4 RN |
Interactioni
Subunit structurei
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit (By similarity).
Interacts with FADD, CFLAR and PEA15 (By similarity).
Interacts with RFFL and RNF34; negatively regulate CASP8 through proteasomal degradation (By similarity).
Interacts with TNFAIP8L2 (By similarity).
Interacts with CASP8AP2 (By similarity).
Interacts with NOL3; decreases CASP8 activity in a mitochondria localization- and phosphorylation-dependent manner and this interaction is dissociated by calcium (PubMed:15383280).
Interacts with UBR2 (By similarity).
By similarity1 PublicationBinary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Q8R2E7 | 2 | EBI-4326675,EBI-4326723 |
GO - Molecular functioni
- death effector domain binding Source: GO_Central
- death receptor binding Source: RGD
- identical protein binding Source: Ensembl
- scaffold protein binding Source: Ensembl
- tumor necrosis factor receptor binding Source: RGD
- ubiquitin protein ligase binding Source: Ensembl
Protein-protein interaction databases
IntActi | Q9JHX4, 2 interactors |
STRINGi | 10116.ENSRNOP00000016613 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 80 | DED 1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 100 – 177 | DED 2PROSITE-ProRule annotationAdd BLAST | 78 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3573 Eukaryota ENOG410ZQIE LUCA |
GeneTreei | ENSGT00940000160319 |
HOGENOMi | HOG000276884 |
InParanoidi | Q9JHX4 |
KOi | K04398 |
OMAi | DHNNKDC |
OrthoDBi | 939331at2759 |
PhylomeDBi | Q9JHX4 |
TreeFami | TF102023 |
Family and domain databases
CDDi | cd00032 CASc, 1 hit |
InterProi | View protein in InterPro IPR029030 Caspase-like_dom_sf IPR033139 Caspase_cys_AS IPR016129 Caspase_his_AS IPR011029 DEATH-like_dom_sf IPR001875 DED_dom IPR002398 Pept_C14 IPR002138 Pept_C14_p10 IPR001309 Pept_C14_p20 IPR015917 Pept_C14A |
PANTHERi | PTHR10454 PTHR10454, 1 hit |
Pfami | View protein in Pfam PF01335 DED, 2 hits |
PRINTSi | PR00376 IL1BCENZYME |
SMARTi | View protein in SMART SM00115 CASc, 1 hit SM00031 DED, 2 hits |
SUPFAMi | SSF47986 SSF47986, 2 hits SSF52129 SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122 CASPASE_CYS, 1 hit PS01121 CASPASE_HIS, 1 hit PS50207 CASPASE_P10, 1 hit PS50208 CASPASE_P20, 1 hit PS50168 DED, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MDFHSCLYDI AERLGNEELA ALKFLCLDHI PQKKQESIND VLVLFQRLQE
60 70 80 90 100
EGMLEEDNLS FLKELLFHIS RRDLLSRVLK SSPEEMVREL QVLGKAQVSA
110 120 130 140 150
YRVMLFKLSE DMDKEDLKSF KFLLITEIPK CKLQDNSSLL DIFVEMEKRT
160 170 180 190 200
ILAENNLVTL KSICFRVNRS LLGRIDDYER SSTERRMSTE GGEELPVSVL
210 220 230 240 250
DEVTIKMQDM WDSPGEQESE SLNSDNVYQM KSKPRGYCLI FNNNNFSKAR
260 270 280 290 300
EDIPKLSNMR DRKGTNYDEE ALSKTFKELH FEIVSFSDCT ASQIHEVLVS
310 320 330 340 350
YQSKDHKGKD CFICCILSHG DKGIVYGTDG KEASIYELTS YFTGSKCPSL
360 370 380 390 400
AGKPKIFFIQ ACQGNNFQKA VPVPDETGLE QEHVLEEDSS SYKNYIPDEA
410 420 430 440 450
DFLLGMATVK NCVSYRDPTR GTWYIQSLCQ SLRERCPRGE DILSILTGVN
460 470 480
YDVSNKDNPR NMGKQMPQPI FTLRKKLFFP PN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF279308 mRNA Translation: AAF87778.1 AF288372 mRNA Translation: AAK83055.1 AABR06060567 Genomic DNA No translation available. AABR06060568 Genomic DNA No translation available. CH473965 Genomic DNA Translation: EDL98978.1 CH473965 Genomic DNA Translation: EDL98979.1 |
RefSeqi | NP_071613.1, NM_022277.1 XP_006245077.1, XM_006245015.3 |
Genome annotation databases
Ensembli | ENSRNOT00000016613; ENSRNOP00000016613; ENSRNOG00000012331 ENSRNOT00000084498; ENSRNOP00000069982; ENSRNOG00000012331 |
GeneIDi | 64044 |
KEGGi | rno:64044 |
UCSCi | RGD:620945 rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF279308 mRNA Translation: AAF87778.1 AF288372 mRNA Translation: AAK83055.1 AABR06060567 Genomic DNA No translation available. AABR06060568 Genomic DNA No translation available. CH473965 Genomic DNA Translation: EDL98978.1 CH473965 Genomic DNA Translation: EDL98979.1 |
RefSeqi | NP_071613.1, NM_022277.1 XP_006245077.1, XM_006245015.3 |
3D structure databases
SMRi | Q9JHX4 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9JHX4, 2 interactors |
STRINGi | 10116.ENSRNOP00000016613 |
Protein family/group databases
MEROPSi | C14.009 |
PTM databases
iPTMneti | Q9JHX4 |
PhosphoSitePlusi | Q9JHX4 |
Proteomic databases
PaxDbi | Q9JHX4 |
PRIDEi | Q9JHX4 |
Genome annotation databases
Ensembli | ENSRNOT00000016613; ENSRNOP00000016613; ENSRNOG00000012331 ENSRNOT00000084498; ENSRNOP00000069982; ENSRNOG00000012331 |
GeneIDi | 64044 |
KEGGi | rno:64044 |
UCSCi | RGD:620945 rat |
Organism-specific databases
CTDi | 841 |
RGDi | 620945 Casp8 |
Phylogenomic databases
eggNOGi | KOG3573 Eukaryota ENOG410ZQIE LUCA |
GeneTreei | ENSGT00940000160319 |
HOGENOMi | HOG000276884 |
InParanoidi | Q9JHX4 |
KOi | K04398 |
OMAi | DHNNKDC |
OrthoDBi | 939331at2759 |
PhylomeDBi | Q9JHX4 |
TreeFami | TF102023 |
Enzyme and pathway databases
BRENDAi | 3.4.22.61 5301 |
Reactomei | R-RNO-111465 Apoptotic cleavage of cellular proteins R-RNO-168638 NOD1/2 Signaling Pathway R-RNO-264870 Caspase-mediated cleavage of cytoskeletal proteins R-RNO-3371378 Regulation by c-FLIP R-RNO-5213460 RIPK1-mediated regulated necrosis R-RNO-5218900 CASP8 activity is inhibited R-RNO-5357905 Regulation of TNFR1 signaling R-RNO-5660668 CLEC7A/inflammasome pathway R-RNO-69416 Dimerization of procaspase-8 R-RNO-75108 Activation, myristolyation of BID and translocation to mitochondria R-RNO-75153 Apoptotic execution phase R-RNO-75157 FasL/ CD95L signaling R-RNO-75158 TRAIL signaling |
Miscellaneous databases
PROi | PR:Q9JHX4 |
Gene expression databases
Bgeei | ENSRNOG00000012331 Expressed in 10 organ(s), highest expression level in colon |
Genevisiblei | Q9JHX4 RN |
Family and domain databases
CDDi | cd00032 CASc, 1 hit |
InterProi | View protein in InterPro IPR029030 Caspase-like_dom_sf IPR033139 Caspase_cys_AS IPR016129 Caspase_his_AS IPR011029 DEATH-like_dom_sf IPR001875 DED_dom IPR002398 Pept_C14 IPR002138 Pept_C14_p10 IPR001309 Pept_C14_p20 IPR015917 Pept_C14A |
PANTHERi | PTHR10454 PTHR10454, 1 hit |
Pfami | View protein in Pfam PF01335 DED, 2 hits |
PRINTSi | PR00376 IL1BCENZYME |
SMARTi | View protein in SMART SM00115 CASc, 1 hit SM00031 DED, 2 hits |
SUPFAMi | SSF47986 SSF47986, 2 hits SSF52129 SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122 CASPASE_CYS, 1 hit PS01121 CASPASE_HIS, 1 hit PS50207 CASPASE_P10, 1 hit PS50208 CASPASE_P20, 1 hit PS50168 DED, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CASP8_RAT | |
Accessioni | Q9JHX4Primary (citable) accession number: Q9JHX4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 2015 |
Last sequence update: | October 1, 2000 | |
Last modified: | November 13, 2019 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families