UniProtKB - Q9JHW9 (AL1A3_MOUSE)
Protein
Aldehyde dehydrogenase family 1 member A3
Gene
Aldh1a3
Organism
Mus musculus (Mouse)
Status
Functioni
NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid (PubMed:11044606, PubMed:11013254, PubMed:14623956). Has high activity with all-trans retinal, and has much lower in vitro activity with acetaldehyde (By similarity). Required for the biosynthesis of normal levels of retinoic acid in the embryonic ocular and nasal regions; retinoic acid is required for normal embryonic development of the eye and the nasal region (PubMed:14623956).By similarity3 Publications
Catalytic activityi
- EC:1.2.1.362 Publications
Kineticsi
- KM=0.33 µM for all-trans retinal1 Publication
- Vmax=58 nmol/min/mg enzyme for all-trans retinal1 Publication
: retinol metabolism Pathwayi
This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.3 PublicationsView all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 181 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 204 | NADBy similarity | 1 | |
Binding sitei | 207 | NADBy similarity | 1 | |
Active sitei | 280 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 314 | NucleophilePROSITE-ProRule annotation | 1 | |
Binding sitei | 361 | NADBy similarity | 1 | |
Binding sitei | 411 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 257 – 262 | NADBy similarity | 6 |
GO - Molecular functioni
- 3-chloroallyl aldehyde dehydrogenase activity Source: MGI
- aldehyde dehydrogenase (NAD+) activity Source: MGI
- aldehyde dehydrogenase [NAD(P)+] activity Source: MGI
- NAD+ binding Source: MGI
- protein homodimerization activity Source: MGI
- retinal dehydrogenase activity Source: UniProtKB
- thyroid hormone binding Source: MGI
GO - Biological processi
- embryonic camera-type eye development Source: UniProtKB
- embryonic eye morphogenesis Source: MGI
- face development Source: MGI
- Harderian gland development Source: UniProtKB
- inner ear morphogenesis Source: MGI
- locomotory behavior Source: MGI
- neuromuscular process controlling balance Source: MGI
- nose development Source: UniProtKB
- nucleus accumbens development Source: MGI
- olfactory pit development Source: MGI
- optic cup morphogenesis involved in camera-type eye development Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of retinoic acid receptor signaling pathway Source: MGI
- protein homotetramerization Source: UniProtKB
- retinal metabolic process Source: MGI
- retinoic acid biosynthetic process Source: MGI
- retinoic acid metabolic process Source: MGI
- retinol metabolic process Source: UniProtKB-UniPathway
- righting reflex Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.2.1.36, 3474 1.2.1.5, 3474 |
Reactomei | R-MMU-5365859, RA biosynthesis pathway |
SABIO-RKi | Q9JHW9 |
UniPathwayi | UPA00912 |
Names & Taxonomyi
Protein namesi | Recommended name: Aldehyde dehydrogenase family 1 member A3 (EC:1.2.1.362 Publications)Alternative name(s): Aldehyde dehydrogenase 61 Publication Retinaldehyde dehydrogenase 3 Short name: RALDH-31 Publication Short name: RalDH31 Publication |
Gene namesi | Name:Aldh1a3 Synonyms:Aldh6, Raldh3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1861722, Aldh1a3 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutant mice are born at the expected Mendelian rate, but all die within 10 hours after birth (PubMed:14623956, PubMed:23536097). Lethality is due to respiratory distress, caused by choanal atresia, i.e. the lack of communication between the nasal and oral cavities. Mutant embryos at 11.5 dpc lack detectable retinoic acid in the ventral retina, nasal epithelium and in the nasolacrimal groove. At 14.5 dpc mutant embryos display shortening of the ventral retina associated with lens rotation and persistence of the retrolenticular membrane, indicative of retinoic acid deficiency. Still, at 18.5 dpc the ventral retina appears normal. Embryos at 18.5 dpc lack Harderian glands, and display multiple malformations in the nasal region, including choanal atresia, lack of maxillary sinuses and nasolacrimal ducts (PubMed:14623956). Oral gavage of pregnant females with retinoic acid prevents choanal atresia and other malformations of the nasal region (PubMed:14623956, PubMed:23536097). Females that were fed retinoic acid give birth to pups with malformations of the inner ear vestibular organ, causing repetitive circling behavior with head tilting (PubMed:23536097). Likewise, mice display impaired ability in crossing a beam without slipping and an impaired ability to swim (PubMed:23536097).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000056479 | 2 – 512 | Aldehyde dehydrogenase family 1 member A3Add BLAST | 511 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | Q9JHW9 |
MaxQBi | Q9JHW9 |
PaxDbi | Q9JHW9 |
PRIDEi | Q9JHW9 |
PTM databases
iPTMneti | Q9JHW9 |
PhosphoSitePlusi | Q9JHW9 |
Expressioni
Tissue specificityi
Detected in embryonic head (at protein level) (PubMed:14623956). Ventral retina.5 Publications
Developmental stagei
In mouse embryos, RALDH3 expression is first noticed in the ventral optic eminence at 8.75 dpc, then in the optic vesicle/cup, otic vesicle and olfactory placode/pit from 9.5 dpc to 11.5 dpc.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000015134, Expressed in decidua and 288 other tissues |
Genevisiblei | Q9JHW9, MM |
Interactioni
Subunit structurei
Homotetramer.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 208193, 1 interactor |
STRINGi | 10090.ENSMUSP00000015278 |
Miscellaneous databases
RNActi | Q9JHW9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldehyde dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG2450, Eukaryota |
GeneTreei | ENSGT00940000158815 |
HOGENOMi | CLU_005391_0_1_1 |
InParanoidi | Q9JHW9 |
OMAi | GTYAINW |
OrthoDBi | 153834at2759 |
PhylomeDBi | Q9JHW9 |
TreeFami | TF300455 |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR012394, Aldehyde_DH_NAD(P) |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036492, ALDH, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9JHW9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATTNGAVEN GQPDGKPPAL PRPIRNLEVK FTKIFINNDW HESKSGRKFA
60 70 80 90 100
TYNPSTLEKI CEVEEGDKPD VDKAVEAAQA AFQRGSPWRR LDALSRGQLL
110 120 130 140 150
HQLADLVERD RAILATLETM DTGKPFLHAF FVDLEGCIKT FRYFAGWADK
160 170 180 190 200
IQGRTIPTDD NVVCFTRHEP IGVCGAITPW NFPLLMLAWK LAPALCCGNT
210 220 230 240 250
VVLKPAEQTP LTALYLASLI KEVGFPPGVV NIVPGFGPTV GAAISSHPQI
260 270 280 290 300
NKIAFTGSTE VGKLVREAAS RSNLKRVTLE LGGKNPCIVC ADADLDLAVE
310 320 330 340 350
CAHQGVFFNQ GQCCTAASRV FVEEQVYGEF VRRSVEFAKK RPVGDPFDAK
360 370 380 390 400
TEQGPQIDQK QFDKILELIE SGKKEGAKLE CGGSAMEDRG LFIKPTVFSD
410 420 430 440 450
VTDNMRIAKE EIFGPVQPIL KFKNLEEVIK RANSTDYGLT AAVFTKNLDK
460 470 480 490 500
ALKLAAALES GTVWINCYNA FYAQAPFGGF KMSGNGRELG EYALAEYTEV
510
KTVTIKLEEK NP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UWP3 | G3UWP3_MOUSE | Aldehyde dehydrogenase family 1 mem... | Aldh1a3 | 277 | Annotation score: | ||
A0A140LIF9 | A0A140LIF9_MOUSE | Aldehyde dehydrogenase family 1 mem... | Aldh1a3 | 176 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 91 | L → V in AAF67736 (PubMed:10906479).Curated | 1 | |
Sequence conflicti | 208 | Q → R in AAG33935 (PubMed:11013254).Curated | 1 | |
Sequence conflicti | 223 | V → E in AAF67736 (PubMed:10906479).Curated | 1 | |
Sequence conflicti | 341 | R → S in AAG33935 (PubMed:11013254).Curated | 1 | |
Sequence conflicti | 407 | I → R in AAF67736 (PubMed:10906479).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF253409 mRNA Translation: AAF67736.1 AF280404 mRNA Translation: AAF86980.1 AF246711 mRNA Translation: AAG38488.1 AF152359 mRNA Translation: AAG33935.1 BC058277 mRNA Translation: AAH58277.1 |
CCDSi | CCDS21345.1 |
RefSeqi | NP_444310.3, NM_053080.3 |
Genome annotation databases
Ensembli | ENSMUST00000015278; ENSMUSP00000015278; ENSMUSG00000015134 |
GeneIDi | 56847 |
KEGGi | mmu:56847 |
UCSCi | uc009hhi.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF253409 mRNA Translation: AAF67736.1 AF280404 mRNA Translation: AAF86980.1 AF246711 mRNA Translation: AAG38488.1 AF152359 mRNA Translation: AAG33935.1 BC058277 mRNA Translation: AAH58277.1 |
CCDSi | CCDS21345.1 |
RefSeqi | NP_444310.3, NM_053080.3 |
3D structure databases
SMRi | Q9JHW9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208193, 1 interactor |
STRINGi | 10090.ENSMUSP00000015278 |
PTM databases
iPTMneti | Q9JHW9 |
PhosphoSitePlusi | Q9JHW9 |
Proteomic databases
jPOSTi | Q9JHW9 |
MaxQBi | Q9JHW9 |
PaxDbi | Q9JHW9 |
PRIDEi | Q9JHW9 |
Protocols and materials databases
Antibodypediai | 43944, 211 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000015278; ENSMUSP00000015278; ENSMUSG00000015134 |
GeneIDi | 56847 |
KEGGi | mmu:56847 |
UCSCi | uc009hhi.2, mouse |
Organism-specific databases
CTDi | 220 |
MGIi | MGI:1861722, Aldh1a3 |
Phylogenomic databases
eggNOGi | KOG2450, Eukaryota |
GeneTreei | ENSGT00940000158815 |
HOGENOMi | CLU_005391_0_1_1 |
InParanoidi | Q9JHW9 |
OMAi | GTYAINW |
OrthoDBi | 153834at2759 |
PhylomeDBi | Q9JHW9 |
TreeFami | TF300455 |
Enzyme and pathway databases
UniPathwayi | UPA00912 |
BRENDAi | 1.2.1.36, 3474 1.2.1.5, 3474 |
Reactomei | R-MMU-5365859, RA biosynthesis pathway |
SABIO-RKi | Q9JHW9 |
Miscellaneous databases
BioGRID-ORCSi | 56847, 1 hit in 17 CRISPR screens |
ChiTaRSi | Aldh1a3, mouse |
PROi | PR:Q9JHW9 |
RNActi | Q9JHW9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015134, Expressed in decidua and 288 other tissues |
Genevisiblei | Q9JHW9, MM |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR012394, Aldehyde_DH_NAD(P) |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036492, ALDH, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AL1A3_MOUSE | |
Accessioni | Q9JHW9Primary (citable) accession number: Q9JHW9 Secondary accession number(s): Q9EQP7, Q9JI72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families