UniProtKB - Q9JHL4 (DBNL_RAT)
Protein
Drebrin-like protein
Gene
Dbnl
Organism
Rattus norvegicus (Rat)
Status
Functioni
Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Required for the formation of organized podosome rosettes. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (By similarity). Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself.By similarity4 Publications
GO - Molecular functioni
- actin binding Source: UniProtKB
- actin filament binding Source: UniProtKB
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- neuron projection morphogenesis Source: UniProtKB
- podosome assembly Source: UniProtKB
- positive regulation of axon extension Source: GO_Central
- positive regulation of dendritic spine morphogenesis Source: GO_Central
- postsynaptic actin cytoskeleton organization Source: GO_Central
- Rac protein signal transduction Source: UniProtKB
- receptor-mediated endocytosis Source: InterPro
- regulation of actin filament polymerization Source: GO_Central
- ruffle assembly Source: InterPro
- synapse assembly Source: UniProtKB
Keywordsi
Molecular function | Actin-binding |
Biological process | Adaptive immunity, Endocytosis, Immunity, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-264870, Caspase-mediated cleavage of cytoskeletal proteins R-RNO-6794361, Neurexins and neuroligins R-RNO-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Drebrin-like proteinAlternative name(s): Actin-binding protein 1 Short name: Abp1 SH3 domain-containing protein 7 |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 70941, Dbnl |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein ; Cytoplasmic side
Cytosol
- cytosol 1 Publication
Golgi apparatus
- Golgi apparatus membrane By similarity; Peripheral membrane protein ; Cytoplasmic side
Cytoskeleton
- cytoskeleton 1 Publication
Endosome
- Early endosome By similarity
Other locations
- lamellipodium 1 Publication
- ruffle 1 Publication
- cell cortex 1 Publication
- synapse 1 Publication
- Perikaryon By similarity
- neuron projection By similarity
- dendrite 1 Publication
- postsynaptic density 1 Publication
- clathrin-coated vesicle membrane By similarity; Peripheral membrane protein ; Cytoplasmic side
- podosome By similarity
Note: Associates with lamellipodial actin and membrane ruffles. Colocalizes with actin and cortactin at podosome dots and podosome rosettes.1 Publication
Cytoskeleton
- cortical actin cytoskeleton Source: GO_Central
- podosome Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB-SubCell
Endosome
- early endosome Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: GO_Central
- postsynaptic membrane Source: GO_Central
Other locations
- cell cortex Source: UniProtKB
- clathrin-coated vesicle membrane Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
- dendrite Source: RGD
- lamellipodium Source: UniProtKB
- perikaryon Source: UniProtKB-SubCell
- postsynaptic density Source: RGD
- ruffle Source: UniProtKB
- site of polarized growth Source: GO_Central
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079795 | 1 – 436 | Drebrin-like proteinAdd BLAST | 436 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 26 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 160 | PhosphoserineBy similarity | 1 | |
Modified residuei | 176 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 277 | PhosphoserineBy similarity | 1 | |
Modified residuei | 280 | PhosphoserineBy similarity | 1 | |
Modified residuei | 283 | PhosphoserineBy similarity | 1 | |
Modified residuei | 291 | PhosphoserineCombined sources | 1 | |
Modified residuei | 296 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 299 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 311 | PhosphoserineCombined sources | 1 | |
Modified residuei | 340 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 350 | PhosphotyrosineBy similarity | 1 |
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 367 – 368 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q9JHL4 |
PaxDbi | Q9JHL4 |
PRIDEi | Q9JHL4 |
PTM databases
iPTMneti | Q9JHL4 |
PhosphoSitePlusi | Q9JHL4 |
Expressioni
Tissue specificityi
Detected in brain (at protein level). Widely expressed in brain with highest levels in hippocampus and cerebral cortex. Located primarily in dendrites and, in moderate amounts, in cell bodies. Isoform 1 and isoform 3 are the predominant isoforms in brain.3 Publications
Gene expression databases
Bgeei | ENSRNOG00000012378, Expressed in spleen and 21 other tissues |
Genevisiblei | Q9JHL4, RN |
Interactioni
Subunit structurei
Interacts with FGD1, MAP4K1 and PRAM1 (By similarity).
Interacts with ANKRD54.
Interacts with WASL and WIPF1 (By similarity).
Interacts with SHANK2 and SHANK3.
Interacts with both COBL and PACSIN1.
Interacts with DNM1 and SYN1.
By similarity5 PublicationsGO - Molecular functioni
- actin binding Source: UniProtKB
- actin filament binding Source: UniProtKB
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249745, 1 interactor |
IntActi | Q9JHL4, 2 interactors |
MINTi | Q9JHL4 |
STRINGi | 10116.ENSRNOP00000017375 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 133 | ADF-HPROSITE-ProRule annotationAdd BLAST | 132 | |
Domaini | 377 – 436 | SH3PROSITE-ProRule annotationAdd BLAST | 60 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 178 – 232 | Sequence analysisAdd BLAST | 55 |
Domaini
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
Sequence similaritiesi
Belongs to the ABP1 family.Curated
Keywords - Domaini
Coiled coil, SH3 domainPhylogenomic databases
eggNOGi | KOG3655, Eukaryota |
GeneTreei | ENSGT00940000156732 |
HOGENOMi | CLU_013085_0_1_1 |
InParanoidi | Q9JHL4 |
OMAi | GYEGQTN |
OrthoDBi | 885776at2759 |
PhylomeDBi | Q9JHL4 |
TreeFami | TF318935 |
Family and domain databases
CDDi | cd11960, SH3_Abp1_eu, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR029923, Dbnl IPR035717, Drebrin-like_SH3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10829:SF12, PTHR10829:SF12, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit PF14604, SH3_9, 1 hit |
SMARTi | View protein in SMART SM00102, ADF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit PS50002, SH3, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketIsoform 11 Publication (identifier: Q9JHL4-1) [UniParc]FASTAAdd to basket
Also known as: SH3P7r11 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVNLSRNGP ALQEAYVRVV TEKSPTDWAL FTYEGNSNDI RVAGTGEGGL
60 70 80 90 100
EELVEELNSG KVMYAFCRVK DPNSGLPKFV LINWTGEGVN DVRKGACANH
110 120 130 140 150
VSTMANFLKG AHVTINARAE EDVEPECIME KVAKASGANY SFHKESSCFQ
160 170 180 190 200
DVGPQAPVGS VYQKTNAVSE IKRVGKDNFW AKAEKEEENR RLEEKRRAEE
210 220 230 240 250
EKQRLEEERR ERELQEAARR EQRYQEQHRS AGPPSPSSRT GELEQEVVSR
260 270 280 290 300
SRQEWESAGQ QAPHPREIFK QKERAMSTTS VSSSQPGKLR SPFLQKQFTQ
310 320 330 340 350
PEASYGREPT SPVSRPAAGV CEELAPSTPP SAQTDDEPTY EVPSEQETLY
360 370 380 390 400
EEPPPVQQPG AGSGHIDNYM QSQDLSGQGL CARALYDYQA ADDTEISFDP
410 420 430
ENLITGIEVI DEGWWRGYGP DGHFGMFPAN YVELIE
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050791 | 235 – 238 | Missing in isoform 3 and isoform 4. 1 Publication | 4 | |
Alternative sequenceiVSP_050792 | 236 – 238 | Missing in isoform 2. 1 Publication | 3 | |
Alternative sequenceiVSP_050793 | 256 | Missing in isoform 4. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB038364 mRNA Translation: BAA90866.1 AB038365 mRNA Translation: BAA90867.1 AB039818 mRNA Translation: BAA92708.1 AB039819 mRNA Translation: BAA92709.1 AB009346 mRNA Translation: BAA90819.1 BC072483 mRNA Translation: AAH72483.1 |
RefSeqi | NP_001264140.1, NM_001277211.1 [Q9JHL4-1] NP_001264141.1, NM_001277212.1 [Q9JHL4-3] NP_001264142.1, NM_001277213.1 [Q9JHL4-4] NP_112642.1, NM_031352.2 [Q9JHL4-2] |
Genome annotation databases
Ensembli | ENSRNOT00000017375; ENSRNOP00000017375; ENSRNOG00000012378 [Q9JHL4-1] |
GeneIDi | 83527 |
KEGGi | rno:83527 |
UCSCi | RGD:70941, rat [Q9JHL4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB038364 mRNA Translation: BAA90866.1 AB038365 mRNA Translation: BAA90867.1 AB039818 mRNA Translation: BAA92708.1 AB039819 mRNA Translation: BAA92709.1 AB009346 mRNA Translation: BAA90819.1 BC072483 mRNA Translation: AAH72483.1 |
RefSeqi | NP_001264140.1, NM_001277211.1 [Q9JHL4-1] NP_001264141.1, NM_001277212.1 [Q9JHL4-3] NP_001264142.1, NM_001277213.1 [Q9JHL4-4] NP_112642.1, NM_031352.2 [Q9JHL4-2] |
3D structure databases
SMRi | Q9JHL4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249745, 1 interactor |
IntActi | Q9JHL4, 2 interactors |
MINTi | Q9JHL4 |
STRINGi | 10116.ENSRNOP00000017375 |
PTM databases
iPTMneti | Q9JHL4 |
PhosphoSitePlusi | Q9JHL4 |
Proteomic databases
jPOSTi | Q9JHL4 |
PaxDbi | Q9JHL4 |
PRIDEi | Q9JHL4 |
Genome annotation databases
Ensembli | ENSRNOT00000017375; ENSRNOP00000017375; ENSRNOG00000012378 [Q9JHL4-1] |
GeneIDi | 83527 |
KEGGi | rno:83527 |
UCSCi | RGD:70941, rat [Q9JHL4-1] |
Organism-specific databases
CTDi | 28988 |
RGDi | 70941, Dbnl |
Phylogenomic databases
eggNOGi | KOG3655, Eukaryota |
GeneTreei | ENSGT00940000156732 |
HOGENOMi | CLU_013085_0_1_1 |
InParanoidi | Q9JHL4 |
OMAi | GYEGQTN |
OrthoDBi | 885776at2759 |
PhylomeDBi | Q9JHL4 |
TreeFami | TF318935 |
Enzyme and pathway databases
Reactomei | R-RNO-264870, Caspase-mediated cleavage of cytoskeletal proteins R-RNO-6794361, Neurexins and neuroligins R-RNO-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:Q9JHL4 |
Gene expression databases
Bgeei | ENSRNOG00000012378, Expressed in spleen and 21 other tissues |
Genevisiblei | Q9JHL4, RN |
Family and domain databases
CDDi | cd11960, SH3_Abp1_eu, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR029923, Dbnl IPR035717, Drebrin-like_SH3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10829:SF12, PTHR10829:SF12, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit PF14604, SH3_9, 1 hit |
SMARTi | View protein in SMART SM00102, ADF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DBNL_RAT | |
Accessioni | Q9JHL4Primary (citable) accession number: Q9JHL4 Secondary accession number(s): Q9JM66, Q9JM67, Q9JM74 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families