UniProtKB - Q9JHL1 (NHRF2_MOUSE)
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Slc9a3r2
Functioni
GO - Molecular functioni
- beta-catenin binding Source: MGI
- identical protein binding Source: MGI
- low-density lipoprotein particle receptor binding Source: MGI
- molecular adaptor activity Source: MGI
- phosphatase binding Source: UniProtKB
- protein C-terminus binding Source: MGI
- protein-membrane adaptor activity Source: GO_Central
- protein N-terminus binding Source: MGI
- signaling receptor binding Source: MGI
GO - Biological processi
- negative regulation of phosphatidylinositol 3-kinase signaling Source: MGI
- positive regulation of ion transmembrane transport Source: MGI
- protein localization to plasma membrane Source: GO_Central
- sensory perception of sound Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: Na(+)/H(+) exchange regulatory cofactor NHE-RF2Short name: NHERF-2 Alternative name(s): NHE3 kinase A regulatory protein E3KARP Octs2 SRY-interacting protein 1 Short name: SIP-1 Sodium-hydrogen exchanger regulatory factor 2 Solute carrier family 9 isoform A3 regulatory factor 2 Tyrosine kinase activator protein 1 Short name: TKA-1 |
Gene namesi | Name:Slc9a3r2 Synonyms:Nherf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1890662, Slc9a3r2 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity
Nucleus
- Nucleus By similarity
Other locations
- Endomembrane system By similarity; Peripheral membrane protein By similarity
Note: Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes. Nuclear, in a punctate pattern (By similarity).By similarity
Nucleus
- nucleus Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- endomembrane system Source: UniProtKB-SubCell
- protein-containing complex Source: MGI
- stereocilium Source: MGI
- stereocilium bundle Source: MGI
Keywords - Cellular componenti
Cell membrane, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096806 | 1 – 337 | Na(+)/H(+) exchange regulatory cofactor NHE-RF2Add BLAST | 337 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 130 | PhosphoserineCombined sources | 1 | |
Modified residuei | 183 | PhosphoserineBy similarity | 1 | |
Modified residuei | 186 | PhosphoserineBy similarity | 1 | |
Modified residuei | 254 | PhosphoserineBy similarity | 1 | |
Modified residuei | 269 | PhosphoserineCombined sources | 1 | |
Modified residuei | 280 | PhosphoserineCombined sources | 1 | |
Modified residuei | 303 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9JHL1 |
PaxDbi | Q9JHL1 |
PeptideAtlasi | Q9JHL1 |
PRIDEi | Q9JHL1 |
ProteomicsDBi | 293554 [Q9JHL1-1] 293555 [Q9JHL1-2] |
PTM databases
iPTMneti | Q9JHL1 |
PhosphoSitePlusi | Q9JHL1 |
Interactioni
Subunit structurei
Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1.
Interacts with SRY. Binds ADRB2, SLC9A3, P2RY1, P2YR2, RDX and LPAR2 (By similarity).
Interacts with MCC (By similarity).
Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity).
Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells (By similarity).
Interacts with SGK1 and KCNJ1/ROMK1 (By similarity).
Interacts (via the PDZ domains) with SLC26A6 (By similarity).
By similarityBinary interactionsi
Hide detailsQ9JHL1
With | #Exp. | IntAct |
---|---|---|
Pdgfrb [P05622] | 2 | EBI-538451,EBI-1554855 |
Pten [O08586] | 3 | EBI-538451,EBI-1186266 |
PTEN [P60484] from Homo sapiens. | 2 | EBI-538451,EBI-696162 |
GO - Molecular functioni
- beta-catenin binding Source: MGI
- identical protein binding Source: MGI
- low-density lipoprotein particle receptor binding Source: MGI
- phosphatase binding Source: UniProtKB
- protein C-terminus binding Source: MGI
- protein N-terminus binding Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 211159, 16 interactors |
IntActi | Q9JHL1, 7 interactors |
MINTi | Q9JHL1 |
STRINGi | 10090.ENSMUSP00000002572 |
Miscellaneous databases
RNActi | Q9JHL1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 91 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 151 – 231 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 81 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3528, Eukaryota |
InParanoidi | Q9JHL1 |
OrthoDBi | 880632at2759 |
PhylomeDBi | Q9JHL1 |
TreeFami | TF350449 |
Family and domain databases
Gene3Di | 2.30.42.10, 2 hits |
InterProi | View protein in InterPro IPR015098, EBP50_C-term IPR017300, NHERF-1/NHERF-2 IPR031197, NHERF-2 IPR001478, PDZ IPR036034, PDZ_sf |
PANTHERi | PTHR14191:SF4, PTHR14191:SF4, 1 hit |
Pfami | View protein in Pfam PF09007, EBP50_C, 1 hit PF00595, PDZ, 2 hits |
PIRSFi | PIRSF037866, EBP50, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 2 hits |
SUPFAMi | SSF50156, SSF50156, 2 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL
60 70 80 90 100
RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTQLLVVDK ETDEELCRRQ
110 120 130 140 150
LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGQKDV NGPPRELRPR
160 170 180 190 200
LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASHSGL RAQDRLIEVN
210 220 230 240 250
GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEEHVEG
260 270 280 290 300
PLPSPVTNGT SPAQLNGGSV CSSRSDLPGS EKDNEDGSTW KRDPFQESGL
310 320 330
HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4IZX2 | A0A0R4IZX2_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Slc9a3r2 | 337 | Annotation score: | ||
Q6NS54 | Q6NS54_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Slc9a3r2 | 316 | Annotation score: | ||
A0A0R4J006 | A0A0R4J006_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Slc9a3r2 | 226 | Annotation score: | ||
A0A3Q4EHM9 | A0A3Q4EHM9_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Slc9a3r2 | 205 | Annotation score: | ||
A0A3Q4EBU8 | A0A3Q4EBU8_MOUSE | Na(+)/H(+) exchange regulatory cofa... | Slc9a3r2 | 231 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | P → L in BAC30366 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 4 | P → L in BAC35980 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 4 | P → L in BAC39282 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 143 | P → S in BAA97568 (Ref. 1) Curated | 1 | |
Sequence conflicti | 143 | P → S in BAA97569 (Ref. 1) Curated | 1 | |
Sequence conflicti | 143 | P → S in BAE41537 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 242 | V → I in BAC30366 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 242 | V → I in BAC35980 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 242 | V → I in BAC39282 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 242 | V → I in AAH65778 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 326 | D → A in BAB22185 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009379 | 1 – 111 | Missing in isoform 2. 2 PublicationsAdd BLAST | 111 | |
Alternative sequenceiVSP_009380 | 112 – 138 | LPPAH…DTGQK → MARSRTSMLPASAPGAPPVN SQLGLTQ in isoform 2. 2 PublicationsAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB026489 mRNA Translation: BAA97568.1 AB026490 Genomic DNA Translation: BAA97569.1 AK002557 mRNA Translation: BAB22185.1 AK039491 mRNA Translation: BAC30366.1 AK075813 mRNA Translation: BAC35980.1 AK084801 mRNA Translation: BAC39282.1 AK170057 mRNA Translation: BAE41537.1 BC065778 mRNA Translation: AAH65778.1 |
CCDSi | CCDS28488.1 [Q9JHL1-1] CCDS28489.1 [Q9JHL1-2] |
RefSeqi | NP_075542.2, NM_023055.2 NP_075938.2, NM_023449.3 |
Genome annotation databases
GeneIDi | 65962 |
KEGGi | mmu:65962 |
UCSCi | uc008axj.2, mouse [Q9JHL1-2] uc008axk.2, mouse [Q9JHL1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB026489 mRNA Translation: BAA97568.1 AB026490 Genomic DNA Translation: BAA97569.1 AK002557 mRNA Translation: BAB22185.1 AK039491 mRNA Translation: BAC30366.1 AK075813 mRNA Translation: BAC35980.1 AK084801 mRNA Translation: BAC39282.1 AK170057 mRNA Translation: BAE41537.1 BC065778 mRNA Translation: AAH65778.1 |
CCDSi | CCDS28488.1 [Q9JHL1-1] CCDS28489.1 [Q9JHL1-2] |
RefSeqi | NP_075542.2, NM_023055.2 NP_075938.2, NM_023449.3 |
3D structure databases
SMRi | Q9JHL1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211159, 16 interactors |
IntActi | Q9JHL1, 7 interactors |
MINTi | Q9JHL1 |
STRINGi | 10090.ENSMUSP00000002572 |
PTM databases
iPTMneti | Q9JHL1 |
PhosphoSitePlusi | Q9JHL1 |
Proteomic databases
jPOSTi | Q9JHL1 |
PaxDbi | Q9JHL1 |
PeptideAtlasi | Q9JHL1 |
PRIDEi | Q9JHL1 |
ProteomicsDBi | 293554 [Q9JHL1-1] 293555 [Q9JHL1-2] |
Genome annotation databases
GeneIDi | 65962 |
KEGGi | mmu:65962 |
UCSCi | uc008axj.2, mouse [Q9JHL1-2] uc008axk.2, mouse [Q9JHL1-1] |
Organism-specific databases
CTDi | 9351 |
MGIi | MGI:1890662, Slc9a3r2 |
Phylogenomic databases
eggNOGi | KOG3528, Eukaryota |
InParanoidi | Q9JHL1 |
OrthoDBi | 880632at2759 |
PhylomeDBi | Q9JHL1 |
TreeFami | TF350449 |
Miscellaneous databases
BioGRID-ORCSi | 65962, 3 hits in 52 CRISPR screens |
PROi | PR:Q9JHL1 |
RNActi | Q9JHL1, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 2.30.42.10, 2 hits |
InterProi | View protein in InterPro IPR015098, EBP50_C-term IPR017300, NHERF-1/NHERF-2 IPR031197, NHERF-2 IPR001478, PDZ IPR036034, PDZ_sf |
PANTHERi | PTHR14191:SF4, PTHR14191:SF4, 1 hit |
Pfami | View protein in Pfam PF09007, EBP50_C, 1 hit PF00595, PDZ, 2 hits |
PIRSFi | PIRSF037866, EBP50, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 2 hits |
SUPFAMi | SSF50156, SSF50156, 2 hits |
PROSITEi | View protein in PROSITE PS50106, PDZ, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NHRF2_MOUSE | |
Accessioni | Q9JHL1Primary (citable) accession number: Q9JHL1 Secondary accession number(s): Q3TDR3 Q9DCR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | May 29, 2007 | |
Last modified: | April 7, 2021 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot