UniProtKB - Q9JHL1 (NHRF2_MOUSE)

BLAST

>sp|Q9JHL1|NHRF2_MOUSE Na(+)/H(+) exchange regulatory cofactor NHE-RF2 OS=Mus musculus OX=10090 GN=Slc9a3r2 PE=1 SV=2
MAAPESLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVN
GVNVEGETHHQVVQRIKAVEGQTQLLVVDKETDEELCRRQLTCTEEMAHRGLPPAHNPWE
PKPDWACSGSLGSDTGQKDVNGPPRELRPRLCHLRRGPQGYGFNLHSDKSRPGQYIRSVD
PGSPASHSGLRAQDRLIEVNGQNVEGLRHAEVVARIKAQEDEARLLVVDPETDEHFKRLR
VVPTEEHVEGPLPSPVTNGTSPAQLNGGSVCSSRSDLPGSEKDNEDGSTWKRDPFQESGL
HLSPTAAEAKEKARATRVNKRAPQMDWNRKREIFSNF

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History
Entry version 145 (07 Nov 2018)
Sequence version 2 (29 May 2007)
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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Gene

Slc9a3r2

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity).By similarity

GO - Molecular functionⁱ

beta-catenin binding Source: MGI
low-density lipoprotein particle receptor binding Source: MGI
phosphatase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 16456542
protein-containing complex scaffold activity Source: MGI
protein C-terminus binding Source: MGI
protein N-terminus binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Na(+)/H(+) exchange regulatory cofactor NHE-RF2 Short name: NHERF-2 Alternative name(s): NHE3 kinase A regulatory protein E3KARP Octs2 SRY-interacting protein 1 Short name: SIP-1 Sodium-hydrogen exchanger regulatory factor 2 Solute carrier family 9 isoform A3 regulatory factor 2 Tyrosine kinase activator protein 1 Short name: TKA-1
Gene namesⁱ	Name:Slc9a3r2 Synonyms:Nherf2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:1890662 Slc9a3r2

Keywords - Cellular componentⁱ

Cell membrane, Membrane, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000096806	1 – 337	Na(+)/H(+) exchange regulatory cofactor NHE-RF2Add BLAST		337

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	130	PhosphoserineCombined sources	1
Modified residueⁱ	183	PhosphoserineBy similarity	1
Modified residueⁱ	186	PhosphoserineBy similarity	1
Modified residueⁱ	254	PhosphoserineBy similarity	1
Modified residueⁱ	269	PhosphoserineCombined sources	1
Modified residueⁱ	280	PhosphoserineCombined sources	1
Modified residueⁱ	303	PhosphoserineBy similarity	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q9JHL1
PeptideAtlas More...PeptideAtlasⁱ	Q9JHL1
PRIDEⁱ	Q9JHL1

PTM databases

iPTMnetⁱ	Q9JHL1
PhosphoSitePlusⁱ	Q9JHL1

Interactionⁱ

Subunit structureⁱ

Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Interacts with SRY. Binds ADRB2, SLC9A3, P2RY1, P2YR2, RDX and LPAR2 (By similarity). Interacts with MCC (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells (By similarity). Interacts with SGK1 and KCNJ1/ROMK1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Pdgfrb	P05622	2	EBI-538451,EBI-1554855
PTEN	P60484	2	EBI-538451,EBI-696162	From Homo sapiens.
Pten	O08586	3	EBI-538451,EBI-1186266

GO - Molecular functionⁱ

beta-catenin binding Source: MGI
low-density lipoprotein particle receptor binding Source: MGI
phosphatase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 16456542
protein-containing complex scaffold activity Source: MGI
protein C-terminus binding Source: MGI
protein N-terminus binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	211159, 1 interactor
IntActⁱ	Q9JHL1, 7 interactors
Molecular INTeraction database More...MINTⁱ	Q9JHL1
STRINGⁱ	10090.ENSMUSP00000002572

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9JHL1
SMRⁱ	Q9JHL1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	11 – 91	PDZ 1PROSITE-ProRule annotationAdd BLAST		81
Domainⁱ	151 – 231	PDZ 2PROSITE-ProRule annotationAdd BLAST		81

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	ENOG410ITAC Eukaryota ENOG4111V58 LUCA
HOGENOMⁱ	HOG000089940
HOVERGENⁱ	HBG052616
InParanoidⁱ	Q9JHL1
KEGG Orthology (KO) More...KOⁱ	K13358
PhylomeDBⁱ	Q9JHL1
TreeFamⁱ	TF350449

Family and domain databases

InterProⁱ	View protein in InterPro IPR015098 EBP50_C-term IPR017300 NHERF-1/NHERF-2 IPR031197 NHERF-2 IPR001478 PDZ IPR036034 PDZ_sf
PANTHERⁱ	PTHR14191:SF4 PTHR14191:SF4, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09007 EBP50_C, 2 hits PF00595 PDZ, 2 hits
PIRSFⁱ	PIRSF037866 EBP50, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD283022 EBP50_C-term, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 2 hits
SUPFAMⁱ	SSF50156 SSF50156, 2 hits
PROSITEⁱ	View protein in PROSITE PS50106 PDZ, 2 hits

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9JHL1-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL 
        60         70         80         90        100
RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTQLLVVDK ETDEELCRRQ 
       110        120        130        140        150
LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGQKDV NGPPRELRPR 
       160        170        180        190        200
LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASHSGL RAQDRLIEVN 
       210        220        230        240        250
GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEEHVEG 
       260        270        280        290        300
PLPSPVTNGT SPAQLNGGSV CSSRSDLPGS EKDNEDGSTW KRDPFQESGL 
       310        320        330 
HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF

Length:337

Mass (Da):37,403

Last modified:May 29, 2007 - v2

Checksum:ⁱA28ED245DE453A5F

Isoform 2 (identifier: Q9JHL1-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-111: Missing.
112-138: LPPAHNPWEPKPDWACSGSLGSDTGQK → MARSRTSMLPASAPGAPPVNSQLGLTQ

« Hide

        10         20         30         40         50
MARSRTSMLP ASAPGAPPVN SQLGLTQDVN GPPRELRPRL CHLRRGPQGY 
        60         70         80         90        100
GFNLHSDKSR PGQYIRSVDP GSPASHSGLR AQDRLIEVNG QNVEGLRHAE 
       110        120        130        140        150
VVARIKAQED EARLLVVDPE TDEHFKRLRV VPTEEHVEGP LPSPVTNGTS 
       160        170        180        190        200
PAQLNGGSVC SSRSDLPGSE KDNEDGSTWK RDPFQESGLH LSPTAAEAKE 
       210        220 
KARATRVNKR APQMDWNRKR EIFSNF

Show »

Length:226

Mass (Da):24,861

Checksum:ⁱ09162261FE6ABCD9

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A0R4IZX2	A0A0R4IZX2_MOUSE	Na(+)/H(+) exchange regulatory cofa... Na(+)/H(+) exchange regulatory cofactor NHE-RF	Slc9a3r2	337	Annotation score:
A0A0R4J006	A0A0R4J006_MOUSE	Na(+)/H(+) exchange regulatory cofa... Na(+)/H(+) exchange regulatory cofactor NHE-RF2	Slc9a3r2	226	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	4	P → L in BAC30366 (PubMed:16141072).Curated	1
Sequence conflictⁱ	4	P → L in BAC35980 (PubMed:16141072).Curated	1
Sequence conflictⁱ	4	P → L in BAC39282 (PubMed:16141072).Curated	1
Sequence conflictⁱ	143	P → S in BAA97568 (Ref. 1) Curated	1
Sequence conflictⁱ	143	P → S in BAA97569 (Ref. 1) Curated	1
Sequence conflictⁱ	143	P → S in BAE41537 (PubMed:16141072).Curated	1
Sequence conflictⁱ	242	V → I in BAC30366 (PubMed:16141072).Curated	1
Sequence conflictⁱ	242	V → I in BAC35980 (PubMed:16141072).Curated	1
Sequence conflictⁱ	242	V → I in BAC39282 (PubMed:16141072).Curated	1
Sequence conflictⁱ	242	V → I in AAH65778 (PubMed:15489334).Curated	1
Sequence conflictⁱ	326	D → A in BAB22185 (PubMed:16141072).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_009379	1 – 111	Missing in isoform 2. 2 PublicationsAdd BLAST		111
Alternative sequenceⁱVSP_009380	112 – 138	LPPAH…DTGQK → MARSRTSMLPASAPGAPPVN SQLGLTQ in isoform 2. 2 PublicationsAdd BLAST		27

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB026489 mRNA Translation: BAA97568.1 AB026490 Genomic DNA Translation: BAA97569.1 AK002557 mRNA Translation: BAB22185.1 AK039491 mRNA Translation: BAC30366.1 AK075813 mRNA Translation: BAC35980.1 AK084801 mRNA Translation: BAC39282.1 AK170057 mRNA Translation: BAE41537.1 BC065778 mRNA Translation: AAH65778.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28488.1 [Q9JHL1-1] CCDS28489.1 [Q9JHL1-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_075542.2, NM_023055.2 NP_075938.2, NM_023449.3
UniGeneⁱ	Mm.21587

Genome annotation databases

GeneIDⁱ	65962
KEGGⁱ	mmu:65962
UCSC genome browser More...UCSCⁱ	uc008axj.2 mouse [Q9JHL1-2] uc008axk.2 mouse [Q9JHL1-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9JHL1	Isoform 2 of Na(+)/H(+) exchange regulatory cofactor NHE-RF2		HUMAN		326	UniRef90_Q15599-2
Na(+)/H(+) exchange regulatory cofactor NHE-RF2		RAT		337
Na(+)/H(+) exchange regulatory cofactor NHE-RF		BOVIN		382
Na(+)/H(+) exchange regulatory cofactor NHE-RF		Enhydra lutris kenyoni		338
Na(+)/H(+) exchange regulatory cofactor NHE-RF		Florida manatee		338
+88
Q9JHL1-2	Na(+)/H(+) exchange regulatory cofactor NHE-RF2 isoform X2		MESAU		226	UniRef90_Q9JHL1-2
Na(+)/H(+) exchange regulatory cofactor NHE-RF2		MOUSE		226
UPI000017FE18		RAT		226
UPI00022F4718		CRIGR		226
UPI000A304A99		MUSPA		226
+3

Protein	Similar proteins		Species	Score	Length	Source
Q9JHL1	Na(+)/H(+) exchange regulatory cofactor NHE-RF2	)	HUMAN		337	UniRef50_Q15599
Na(+)/H(+) exchange regulatory cofactor NHE-RF2		RAT		337
Na(+)/H(+) exchange regulatory cofactor NHE-RF		MACMU		335
Na(+)/H(+) exchange regulatory cofactor NHE-RF		RHIBE		331
SLC9A3 regulator 2		AILME		266
+151
Q9JHL1-2	Na(+)/H(+) exchange regulatory cofactor NHE-RF2 isoform X2		MESAU		226	UniRef50_Q9JHL1-2
Na(+)/H(+) exchange regulatory cofactor NHE-RF2		MOUSE		226
Na(+)/H(+) exchange regulatory cofactor NHE-RF2 isoform X3		Florida manatee		226
Na(+)/H(+) exchange regulatory cofactor NHE-RF2-like		SALSA		235
Na(+)/H(+) exchange regulatory cofactor NHE-RF2 isoform X1		ERIEU		226
+14

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB026489 mRNA Translation: BAA97568.1 AB026490 Genomic DNA Translation: BAA97569.1 AK002557 mRNA Translation: BAB22185.1 AK039491 mRNA Translation: BAC30366.1 AK075813 mRNA Translation: BAC35980.1 AK084801 mRNA Translation: BAC39282.1 AK170057 mRNA Translation: BAE41537.1 BC065778 mRNA Translation: AAH65778.1
CCDSⁱ	CCDS28488.1 [Q9JHL1-1] CCDS28489.1 [Q9JHL1-2]
RefSeqⁱ	NP_075542.2, NM_023055.2 NP_075938.2, NM_023449.3
UniGeneⁱ	Mm.21587

3D structure databases

ProteinModelPortalⁱ	Q9JHL1
SMRⁱ	Q9JHL1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	211159, 1 interactor
IntActⁱ	Q9JHL1, 7 interactors
MINTⁱ	Q9JHL1
STRINGⁱ	10090.ENSMUSP00000002572

PTM databases

iPTMnetⁱ	Q9JHL1
PhosphoSitePlusⁱ	Q9JHL1

Proteomic databases

PaxDbⁱ	Q9JHL1
PeptideAtlasⁱ	Q9JHL1
PRIDEⁱ	Q9JHL1

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	65962
KEGGⁱ	mmu:65962
UCSCⁱ	uc008axj.2 mouse [Q9JHL1-2] uc008axk.2 mouse [Q9JHL1-1]

Organism-specific databases

CTDⁱ	9351
MGIⁱ	MGI:1890662 Slc9a3r2

Phylogenomic databases

eggNOGⁱ	ENOG410ITAC Eukaryota ENOG4111V58 LUCA
HOGENOMⁱ	HOG000089940
HOVERGENⁱ	HBG052616
InParanoidⁱ	Q9JHL1
KOⁱ	K13358
PhylomeDBⁱ	Q9JHL1
TreeFamⁱ	TF350449

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9JHL1
SOURCEⁱ	Search...

Family and domain databases

InterProⁱ	View protein in InterPro IPR015098 EBP50_C-term IPR017300 NHERF-1/NHERF-2 IPR031197 NHERF-2 IPR001478 PDZ IPR036034 PDZ_sf
PANTHERⁱ	PTHR14191:SF4 PTHR14191:SF4, 1 hit
Pfamⁱ	View protein in Pfam PF09007 EBP50_C, 2 hits PF00595 PDZ, 2 hits
PIRSFⁱ	PIRSF037866 EBP50, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD283022 EBP50_C-term, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 2 hits
SUPFAMⁱ	SSF50156 SSF50156, 2 hits
PROSITEⁱ	View protein in PROSITE PS50106 PDZ, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NHRF2_MOUSE
Accessionⁱ	Q9JHL1Primary (citable) accession number: Q9JHL1 Secondary accession number(s): Q3TDR3 , Q6P074, Q8BGL9, Q8BW05, Q9DCR6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
	Last sequence update:	May 29, 2007
	Last modified:	November 7, 2018
	This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9JHL1 (NHRF2_MOUSE)

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Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Slc9a3r2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Nucleus

Other locations

Nucleus

Plasma Membrane

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ