UniProtKB - Q9JHI9 (S40A1_MOUSE)
Solute carrier family 40 member 1
Slc40a1
Functioni
Major iron transporter that plays a key role in balancing cellular and systemic iron levels. Transports iron from intestinal, splenic, and hepatic cells into the blood to provide iron to other tissues (PubMed:30213870).
Controls therefore dietary iron uptake, iron recycling by macrophages, and release of iron stores in hepatocytes (PubMed:16054062).
When iron is in excess hepcidin/HAMP levels increase, resulting in a degradation of ferroportin/SLC40A1 limiting the iron efflux to plasma (By similarity).
By similarity2 PublicationsCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 39 | Iron 1By similarity | 1 | |
Metal bindingi | 43 | Iron 1By similarity | 1 | |
Metal bindingi | 326 | Iron 2By similarity | 1 | |
Metal bindingi | 506 | Iron 2By similarity | 1 |
GO - Molecular functioni
- ferrous iron transmembrane transporter activity Source: BHF-UCL
- identical protein binding Source: IntAct
- iron ion transmembrane transporter activity Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- peptide hormone binding Source: BHF-UCL
GO - Biological processi
- cellular iron ion homeostasis Source: MGI
- endothelium development Source: MGI
- iron ion export across plasma membrane Source: ARUK-UCL
- iron ion homeostasis Source: MGI
- iron ion transmembrane transport Source: MGI
- iron ion transport Source: MGI
- lymphocyte homeostasis Source: MGI
- multicellular organismal iron ion homeostasis Source: BHF-UCL
- negative regulation of apoptotic process Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription from RNA polymerase II promoter in response to iron Source: MGI
- spleen development Source: MGI
- spleen trabecula formation Source: MGI
Keywordsi
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-425410, Metal ion SLC transporters R-MMU-917937, Iron uptake and transport |
Protein family/group databases
TCDBi | 2.A.100.1.1, the ferroportin (fpn) family |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 40 member 1Alternative name(s): Ferroportin-1 Iron-regulated transporter 1 Metal transporter protein 1 Short name: MTP1 |
Gene namesi | Name:Slc40a1 Synonyms:Fpn1, Ireg1, Slc11a3, Slc39a1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1315204, Slc40a1 |
VEuPathDBi | HostDB:ENSMUSG00000025993 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Localized to the basolateral membrane of polarized epithelial cells.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- basolateral plasma membrane Source: BHF-UCL
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
- synaptic vesicle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 23 | CytoplasmicBy similarityAdd BLAST | 23 | |
Transmembranei | 24 – 53 | HelicalBy similarityAdd BLAST | 30 | |
Topological domaini | 54 – 57 | ExtracellularBy similarity | 4 | |
Transmembranei | 58 – 84 | HelicalBy similarityAdd BLAST | 27 | |
Topological domaini | 85 – 87 | CytoplasmicBy similarity | 3 | |
Transmembranei | 88 – 118 | HelicalBy similarityAdd BLAST | 31 | |
Topological domaini | 119 – 126 | ExtracellularBy similarity | 8 | |
Transmembranei | 127 – 162 | HelicalBy similarityAdd BLAST | 36 | |
Topological domaini | 163 – 164 | CytoplasmicBy similarity | 2 | |
Transmembranei | 165 – 195 | HelicalBy similarityAdd BLAST | 31 | |
Topological domaini | 196 – 202 | ExtracellularBy similarity | 7 | |
Transmembranei | 203 – 229 | HelicalBy similarityAdd BLAST | 27 | |
Topological domaini | 230 – 306 | CytoplasmicBy similarityAdd BLAST | 77 | |
Transmembranei | 307 – 333 | HelicalBy similarityAdd BLAST | 27 | |
Topological domaini | 334 – 338 | ExtracellularBy similarity | 5 | |
Transmembranei | 339 – 366 | HelicalBy similarityAdd BLAST | 28 | |
Topological domaini | 367 – 368 | CytoplasmicBy similarity | 2 | |
Transmembranei | 369 – 391 | HelicalBy similarityAdd BLAST | 23 | |
Topological domaini | 392 – 452 | ExtracellularBy similarityAdd BLAST | 61 | |
Transmembranei | 453 – 482 | HelicalBy similarityAdd BLAST | 30 | |
Topological domaini | 483 – 487 | CytoplasmicBy similarity | 5 | |
Transmembranei | 488 – 512 | HelicalBy similarityAdd BLAST | 25 | |
Topological domaini | 513 – 515 | ExtracellularBy similarity | 3 | |
Transmembranei | 516 – 541 | HelicalBy similarityAdd BLAST | 26 | |
Topological domaini | 542 – 570 | CytoplasmicBy similarityAdd BLAST | 29 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL4523462 |
GuidetoPHARMACOLOGYi | 1194 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191311 | 1 – 570 | Solute carrier family 40 member 1Add BLAST | 570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 437 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Ubl conjugationProteomic databases
MaxQBi | Q9JHI9 |
PaxDbi | Q9JHI9 |
PRIDEi | Q9JHI9 |
ProteomicsDBi | 256902 |
PTM databases
GlyGeni | Q9JHI9, 4 sites |
iPTMneti | Q9JHI9 |
PhosphoSitePlusi | Q9JHI9 |
SwissPalmi | Q9JHI9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000025993, Expressed in liver and 290 other tissues |
Genevisiblei | Q9JHI9, MM |
Interactioni
Subunit structurei
Identified in a complex with STOM.
Interacts with HAMP; this interaction promotes SLC40A1 rapid ubiquitination.
By similarityBinary interactionsi
Q9JHI9
With | #Exp. | IntAct |
---|---|---|
App [P12023] | 2 | EBI-2931424,EBI-78814 |
itself | 2 | EBI-2931424,EBI-2931424 |
JAK2 [O60674] from Homo sapiens. | 3 | EBI-2931424,EBI-518647 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-58649N |
IntActi | Q9JHI9, 4 interactors |
STRINGi | 10090.ENSMUSP00000027137 |
Miscellaneous databases
RNActi | Q9JHI9, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2601, Eukaryota |
GeneTreei | ENSGT00390000015143 |
HOGENOMi | CLU_020370_1_1_1 |
InParanoidi | Q9JHI9 |
OMAi | NVHELEH |
OrthoDBi | 334057at2759 |
PhylomeDBi | Q9JHI9 |
TreeFami | TF313463 |
Family and domain databases
CDDi | cd17480, MFS_SLC40A1_like, 1 hit |
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR009716, Ferroportin-1 IPR036259, MFS_trans_sf |
PANTHERi | PTHR11660, PTHR11660, 1 hit |
Pfami | View protein in Pfam PF06963, FPN1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MTKARDQTHQ EGCCGSLANY LTSAKFLLYL GHSLSTWGDR MWHFAVSVFL
60 70 80 90 100
VELYGNSLLL TAVYGLVVAG SVLVLGAIIG DWVDKNARLK VAQTSLVVQN
110 120 130 140 150
VSVILCGIIL MMVFLHKNEL LTMYHGWVLT VCYILIITIA NIANLASTAT
160 170 180 190 200
AITIQRDWIV VVAGENRSRL ADMNATIRRI DQLTNILAPM AVGQIMTFGS
210 220 230 240 250
PVIGCGFISG WNLVSMCVEY FLLWKVYQKT PALAVKAALK VEESELKQLT
260 270 280 290 300
SPKDTEPKPL EGTHLMGEKD SNIRELECEQ EPTCASQMAE PFRTFRDGWV
310 320 330 340 350
SYYNQPVFLA GMGLAFLYMT VLGFDCITTG YAYTQGLSGS ILSILMGASA
360 370 380 390 400
ITGIMGTVAF TWLRRKCGLV RTGLFSGLAQ LSCLILCVIS VFMPGSPLDL
410 420 430 440 450
SVSPFEDIRS RFVNVEPVSP TTKIPETVFT TEMHMSNMSN VHEMSTKPIP
460 470 480 490 500
IVSVSLLFAG VIAARIGLWS FDLTVTQLLQ ENVIESERGI INGVQNSMNY
510 520 530 540 550
LLDLLHFIMV ILAPNPEAFG LLVLISVSFV AMGHLMYFRF AQKTLGNQIF
560 570
VCGPDEKEVT DENQPNTSVV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC5H8V4 | C5H8V4_MOUSE | Solute carrier family 40 protein | Slc40a1 | 70 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF215637 mRNA Translation: AAF80987.1 AF216834 Genomic DNA Translation: AAF82036.1 AF226613 mRNA Translation: AAF36696.1 AF231120 mRNA Translation: AAF44329.1 AK008700 mRNA Translation: BAB25840.1 AK032732 mRNA Translation: BAC28001.1 AK083288 mRNA Translation: BAC38844.1 AK144780 mRNA Translation: BAE26063.1 AK147137 mRNA Translation: BAE27707.1 AK159294 mRNA Translation: BAE34969.1 AK159855 mRNA Translation: BAE35431.1 BC003438 mRNA Translation: AAH03438.1 |
CCDSi | CCDS14933.1 |
RefSeqi | NP_058613.2, NM_016917.2 XP_006496200.1, XM_006496137.2 XP_017177198.1, XM_017321709.1 |
Genome annotation databases
Ensembli | ENSMUST00000027137; ENSMUSP00000027137; ENSMUSG00000025993 |
GeneIDi | 53945 |
KEGGi | mmu:53945 |
UCSCi | uc007awu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF215637 mRNA Translation: AAF80987.1 AF216834 Genomic DNA Translation: AAF82036.1 AF226613 mRNA Translation: AAF36696.1 AF231120 mRNA Translation: AAF44329.1 AK008700 mRNA Translation: BAB25840.1 AK032732 mRNA Translation: BAC28001.1 AK083288 mRNA Translation: BAC38844.1 AK144780 mRNA Translation: BAE26063.1 AK147137 mRNA Translation: BAE27707.1 AK159294 mRNA Translation: BAE34969.1 AK159855 mRNA Translation: BAE35431.1 BC003438 mRNA Translation: AAH03438.1 |
CCDSi | CCDS14933.1 |
RefSeqi | NP_058613.2, NM_016917.2 XP_006496200.1, XM_006496137.2 XP_017177198.1, XM_017321709.1 |
3D structure databases
AlphaFoldDBi | Q9JHI9 |
SMRi | Q9JHI9 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-58649N |
IntActi | Q9JHI9, 4 interactors |
STRINGi | 10090.ENSMUSP00000027137 |
Chemistry databases
ChEMBLi | CHEMBL4523462 |
GuidetoPHARMACOLOGYi | 1194 |
Protein family/group databases
TCDBi | 2.A.100.1.1, the ferroportin (fpn) family |
PTM databases
GlyGeni | Q9JHI9, 4 sites |
iPTMneti | Q9JHI9 |
PhosphoSitePlusi | Q9JHI9 |
SwissPalmi | Q9JHI9 |
Proteomic databases
MaxQBi | Q9JHI9 |
PaxDbi | Q9JHI9 |
PRIDEi | Q9JHI9 |
ProteomicsDBi | 256902 |
Protocols and materials databases
Antibodypediai | 34022, 393 antibodies from 33 providers |
DNASUi | 53945 |
Genome annotation databases
Ensembli | ENSMUST00000027137; ENSMUSP00000027137; ENSMUSG00000025993 |
GeneIDi | 53945 |
KEGGi | mmu:53945 |
UCSCi | uc007awu.1, mouse |
Organism-specific databases
CTDi | 30061 |
MGIi | MGI:1315204, Slc40a1 |
VEuPathDBi | HostDB:ENSMUSG00000025993 |
Phylogenomic databases
eggNOGi | KOG2601, Eukaryota |
GeneTreei | ENSGT00390000015143 |
HOGENOMi | CLU_020370_1_1_1 |
InParanoidi | Q9JHI9 |
OMAi | NVHELEH |
OrthoDBi | 334057at2759 |
PhylomeDBi | Q9JHI9 |
TreeFami | TF313463 |
Enzyme and pathway databases
Reactomei | R-MMU-425410, Metal ion SLC transporters R-MMU-917937, Iron uptake and transport |
Miscellaneous databases
BioGRID-ORCSi | 53945, 3 hits in 74 CRISPR screens |
ChiTaRSi | Slc40a1, mouse |
PROi | PR:Q9JHI9 |
RNActi | Q9JHI9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025993, Expressed in liver and 290 other tissues |
Genevisiblei | Q9JHI9, MM |
Family and domain databases
CDDi | cd17480, MFS_SLC40A1_like, 1 hit |
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR009716, Ferroportin-1 IPR036259, MFS_trans_sf |
PANTHERi | PTHR11660, PTHR11660, 1 hit |
Pfami | View protein in Pfam PF06963, FPN1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | S40A1_MOUSE | |
Accessioni | Q9JHI9Primary (citable) accession number: Q9JHI9 Secondary accession number(s): Q3UHZ9 Q9JKP4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families