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Protein

Carboxypeptidase B2

Gene

Cpb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

Catalytic activityi

Release of C-terminal Arg and Lys from a polypeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Activity regulationi

TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-323 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi180Zinc; catalyticBy similarity1
Metal bindingi183Zinc; catalyticBy similarity1
Binding sitei238SubstrateBy similarity1
Metal bindingi309Zinc; catalyticBy similarity1
Binding sitei362SubstrateBy similarity1
Active sitei384Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: MGI
  • metallocarboxypeptidase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
Biological processBlood coagulation, Fibrinolysis, Hemostasis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.20 3474
ReactomeiR-MMU-2022377 Metabolism of Angiotensinogen to Angiotensins
R-MMU-977606 Regulation of Complement cascade

Protein family/group databases

MEROPSiM14.009

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase B2 (EC:3.4.17.20)
Alternative name(s):
Carboxypeptidase R
Short name:
CPR
Carboxypeptidase U
Short name:
CPU
Thrombin-activable fibrinolysis inhibitor
Short name:
TAFI
Gene namesi
Name:Cpb2
Synonyms:Tafi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1891837 Cpb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000000437922 – 113Activation peptideBy similarityAdd BLAST92
ChainiPRO_0000004380114 – 422Carboxypeptidase B2Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi43N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi72N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi177 ↔ 190By similarity
Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi249 ↔ 273By similarity
Disulfide bondi264 ↔ 278By similarity
Glycosylationi322N-linked (GlcNAc...) asparagineSequence analysis1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei323 – 324Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9JHH6
PaxDbiQ9JHH6
PeptideAtlasiQ9JHH6
PRIDEiQ9JHH6

PTM databases

iPTMnetiQ9JHH6
PhosphoSitePlusiQ9JHH6

Expressioni

Tissue specificityi

Plasma; synthesized in the liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000021999 Expressed in 91 organ(s), highest expression level in liver
CleanExiMM_CPB2
GenevisibleiQ9JHH6 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022576

Structurei

3D structure databases

ProteinModelPortaliQ9JHH6
SMRiQ9JHH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 183Substrate bindingBy similarity4
Regioni255 – 256Substrate bindingBy similarity2
Regioni310 – 311Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650 Eukaryota
COG2866 LUCA
GeneTreeiENSGT00760000119103
HOGENOMiHOG000252968
HOVERGENiHBG050815
InParanoidiQ9JHH6
KOiK01300
OMAiNQPAHIR
OrthoDBiEOG091G0HUI
PhylomeDBiQ9JHH6
TreeFamiTF317197

Family and domain databases

CDDicd06246 M14_CPB2, 1 hit
Gene3Di3.30.70.340, 1 hit
InterProiView protein in InterPro
IPR033849 CPB2
IPR036990 M14A-like_propep
IPR003146 M14A_act_pep
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
PF02244 Propep_M14, 1 hit
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JHH6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLHGLGILV AIILYEQHGF AFQSGQVLSA LPRTSRQVQL LQNLTTTYEV
60 70 80 90 100
VLWQPVTAEF IEKKKEVHFF VNASDVDSVK AHLNVSRIPF NVLMNNVEDL
110 120 130 140 150
IEQQTFNDTV SPRASASYYE QYHSLNEIYS WIEVITEQHP DMLQKIYIGS
160 170 180 190 200
SFEKYPLYVL KVSGKEQRIK NAIWIDCGIH AREWISPAFC LWFIGYVTQF
210 220 230 240 250
HGKENLYTRL LRHVDFYIMP VMNVDGYDYT WKKNRMWRKN RSAHKNNRCV
260 270 280 290 300
GTDLNRNFAS KHWCEKGASS SSCSETYCGL YPESEPEVKA VADFLRRNID
310 320 330 340 350
HIKAYISMHS YSQQILFPYS YNRSKSKDHE ELSLVASEAV RAIESINKNT
360 370 380 390 400
RYTHGSGSES LYLAPGGSDD WIYDLGIKYS FTIELRDTGR YGFLLPERYI
410 420
KPTCAEALAA ISKIVWHVIR NT
Length:422
Mass (Da):48,871
Last modified:October 1, 2000 - v1
Checksum:i99113755669D55CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135I → L in AAF00528 (Ref. 3) Curated1
Sequence conflicti277Y → C in AAH89577 (PubMed:15489334).Curated1
Sequence conflicti344 – 346ESI → GSF in AAF00528 (Ref. 3) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021968 mRNA Translation: BAB03402.1
AF164524 mRNA Translation: AAF62385.1
AF186188 mRNA Translation: AAF00528.1
AK004045 mRNA Translation: BAB23141.1
BC089577 mRNA Translation: AAH89577.1
CCDSiCCDS27277.1
RefSeqiNP_062749.2, NM_019775.3
UniGeneiMm.24242

Genome annotation databases

EnsembliENSMUST00000022576; ENSMUSP00000022576; ENSMUSG00000021999
GeneIDi56373
KEGGimmu:56373
UCSCiuc007uqq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021968 mRNA Translation: BAB03402.1
AF164524 mRNA Translation: AAF62385.1
AF186188 mRNA Translation: AAF00528.1
AK004045 mRNA Translation: BAB23141.1
BC089577 mRNA Translation: AAH89577.1
CCDSiCCDS27277.1
RefSeqiNP_062749.2, NM_019775.3
UniGeneiMm.24242

3D structure databases

ProteinModelPortaliQ9JHH6
SMRiQ9JHH6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022576

Protein family/group databases

MEROPSiM14.009

PTM databases

iPTMnetiQ9JHH6
PhosphoSitePlusiQ9JHH6

Proteomic databases

MaxQBiQ9JHH6
PaxDbiQ9JHH6
PeptideAtlasiQ9JHH6
PRIDEiQ9JHH6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022576; ENSMUSP00000022576; ENSMUSG00000021999
GeneIDi56373
KEGGimmu:56373
UCSCiuc007uqq.2 mouse

Organism-specific databases

CTDi1361
MGIiMGI:1891837 Cpb2

Phylogenomic databases

eggNOGiKOG2650 Eukaryota
COG2866 LUCA
GeneTreeiENSGT00760000119103
HOGENOMiHOG000252968
HOVERGENiHBG050815
InParanoidiQ9JHH6
KOiK01300
OMAiNQPAHIR
OrthoDBiEOG091G0HUI
PhylomeDBiQ9JHH6
TreeFamiTF317197

Enzyme and pathway databases

BRENDAi3.4.17.20 3474
ReactomeiR-MMU-2022377 Metabolism of Angiotensinogen to Angiotensins
R-MMU-977606 Regulation of Complement cascade

Miscellaneous databases

ChiTaRSiCapzb mouse
PROiPR:Q9JHH6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021999 Expressed in 91 organ(s), highest expression level in liver
CleanExiMM_CPB2
GenevisibleiQ9JHH6 MM

Family and domain databases

CDDicd06246 M14_CPB2, 1 hit
Gene3Di3.30.70.340, 1 hit
InterProiView protein in InterPro
IPR033849 CPB2
IPR036990 M14A-like_propep
IPR003146 M14A_act_pep
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
PF02244 Propep_M14, 1 hit
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCBPB2_MOUSE
AccessioniPrimary (citable) accession number: Q9JHH6
Secondary accession number(s): Q5EBI3, Q9QZF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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