UniProtKB - Q9JHF9 (LY96_MOUSE)
Lymphocyte antigen 96
Ly96
Functioni
GO - Molecular functioni
- lipopolysaccharide binding Source: InterPro
- lipopolysaccharide immune receptor activity Source: UniProtKB
- Toll-like receptor 4 binding Source: InterPro
GO - Biological processi
- cellular response to lipopolysaccharide Source: UniProtKB
- detection of lipopolysaccharide Source: UniProtKB
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- lipopolysaccharide-mediated signaling pathway Source: UniProtKB
- positive regulation of lipopolysaccharide-mediated signaling pathway Source: MGI
- positive regulation of tumor necrosis factor production Source: UniProtKB
- response to lipopolysaccharide Source: MGI
- toll-like receptor 4 signaling pathway Source: UniProtKB
Keywordsi
Biological process | Immunity, Inflammatory response, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-166016, Toll Like Receptor 4 (TLR4) Cascade R-MMU-166166, MyD88-independent TLR4 cascade R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-5686938, Regulation of TLR by endogenous ligand R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon R-MMU-937041, IKK complex recruitment mediated by RIP1 R-MMU-937072, TRAF6-mediated induction of TAK1 complex within TLR4 complex R-MMU-975163, IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation |
Names & Taxonomyi
Protein namesi | Recommended name: Lymphocyte antigen 96Short name: Ly-96 Alternative name(s): ESOP-11 Publication Protein MD-2 |
Gene namesi | Name:Ly96 Synonyms:Esop1, Md2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1341909, Ly96 |
Subcellular locationi
Extracellular region or secreted
- extracellular space 1 Publication2 Publications
- Secreted 1 Publication
Note: Retained in the extracellular space at the cell surface by interaction with TLR4.1 Publication2 Publications
Extracellular region or secreted
- extracellular space Source: UniProtKB-SubCell
Plasma Membrane
- intrinsic component of plasma membrane Source: UniProtKB
Other locations
- lipopolysaccharide receptor complex Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 126 | F → A: Abolishes dimerization of the TLR4 complex and stimulation of TNF production in response to bacterial lipopolysaccharide. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2384895 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000018620 | 19 – 160 | Lymphocyte antigen 96Add BLAST | 142 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 25 ↔ 51 | Combined sources2 Publications | ||
Glycosylationi | 26 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 37 ↔ 148 | Combined sources2 Publications | ||
Disulfide bondi | 95 ↔ 105 | Combined sources2 Publications | ||
Glycosylationi | 114 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 150 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q9JHF9 |
PaxDbi | Q9JHF9 |
PRIDEi | Q9JHF9 |
PTM databases
GlyGeni | Q9JHF9, 3 sites |
iPTMneti | Q9JHF9 |
PhosphoSitePlusi | Q9JHF9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000025779, Expressed in bone marrow macrophage and 183 other tissues |
Genevisiblei | Q9JHF9, MM |
Interactioni
Subunit structurei
Heterogeneous homopolymer formed from homodimers; disulfide-linked (By similarity). Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (PubMed:10725698, PubMed:20133493, PubMed:24380872). Binds to the extracellular domain of TLR4 (PubMed:10725698, PubMed:20133493, PubMed:24380872, PubMed:17803912, PubMed:22532668). Binds to the extracellular domain of TLR2 (By similarity). Ligand binding induces interaction with TLR4 and oligomerization of the complex (PubMed:20133493, PubMed:24380872, PubMed:22532668).
By similarity5 PublicationsBinary interactionsi
Q9JHF9
With | #Exp. | IntAct |
---|---|---|
Tlr4 [Q9QUK6] | 8 | EBI-1534566,EBI-1534575 |
GO - Molecular functioni
- Toll-like receptor 4 binding Source: InterPro
Protein-protein interaction databases
DIPi | DIP-38572N |
IntActi | Q9JHF9, 1 interactor |
STRINGi | 10090.ENSMUSP00000026881 |
Miscellaneous databases
RNActi | Q9JHF9, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9JHF9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9JHF9 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 119 – 123 | Interaction with lipopolysaccharideBy similarity1 Publication | 5 |
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502SD7W, Eukaryota |
GeneTreei | ENSGT00390000000742 |
InParanoidi | Q9JHF9 |
OMAi | EVICRGY |
OrthoDBi | 1320082at2759 |
PhylomeDBi | Q9JHF9 |
TreeFami | TF335876 |
Family and domain databases
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR039217, LY96 IPR003172, ML_dom |
PANTHERi | PTHR15218, PTHR15218, 1 hit |
Pfami | View protein in Pfam PF02221, E1_DerP2_DerF2, 1 hit |
SMARTi | View protein in SMART SM00737, ML, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MLPFILFSTL LSPILTESEK QQWFCNSSDA IISYSYCDHL KFPISISSEP
60 70 80 90 100
CIRLRGTNGF VHVEFIPRGN LKYLYFNLFI SVNSIELPKR KEVLCHGHDD
110 120 130 140 150
DYSFCRALKG ETVNTSIPFS FEGILFPKGH YRCVAEAIAG DTEEKLFCLN
160
FTIIHRRDVN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WQZ9 | A0A087WQZ9_MOUSE | Lymphocyte antigen 96 | Ly96 | 142 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF168120 mRNA Translation: AAF89634.1 AB018550 mRNA Translation: BAA93619.1 AK019283 mRNA Translation: BAB31645.1 |
CCDSi | CCDS14832.1 |
RefSeqi | NP_001153183.1, NM_001159711.1 NP_058619.1, NM_016923.2 |
Genome annotation databases
Ensembli | ENSMUST00000026881; ENSMUSP00000026881; ENSMUSG00000025779 |
GeneIDi | 17087 |
KEGGi | mmu:17087 |
UCSCi | uc007ajz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF168120 mRNA Translation: AAF89634.1 AB018550 mRNA Translation: BAA93619.1 AK019283 mRNA Translation: BAB31645.1 |
CCDSi | CCDS14832.1 |
RefSeqi | NP_001153183.1, NM_001159711.1 NP_058619.1, NM_016923.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Z64 | X-ray | 2.84 | C | 21-155 | [»] | |
3VQ1 | X-ray | 2.70 | C/D | 17-160 | [»] | |
3VQ2 | X-ray | 2.48 | C/D | 17-160 | [»] | |
5IJB | X-ray | 2.91 | C/D | 19-160 | [»] | |
5IJC | X-ray | 2.57 | C/D | 19-160 | [»] | |
5IJD | X-ray | 2.70 | C/D | 19-160 | [»] | |
SMRi | Q9JHF9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-38572N |
IntActi | Q9JHF9, 1 interactor |
STRINGi | 10090.ENSMUSP00000026881 |
Chemistry databases
ChEMBLi | CHEMBL2384895 |
PTM databases
GlyGeni | Q9JHF9, 3 sites |
iPTMneti | Q9JHF9 |
PhosphoSitePlusi | Q9JHF9 |
Proteomic databases
MaxQBi | Q9JHF9 |
PaxDbi | Q9JHF9 |
PRIDEi | Q9JHF9 |
Protocols and materials databases
Antibodypediai | 12334, 465 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026881; ENSMUSP00000026881; ENSMUSG00000025779 |
GeneIDi | 17087 |
KEGGi | mmu:17087 |
UCSCi | uc007ajz.2, mouse |
Organism-specific databases
CTDi | 23643 |
MGIi | MGI:1341909, Ly96 |
Phylogenomic databases
eggNOGi | ENOG502SD7W, Eukaryota |
GeneTreei | ENSGT00390000000742 |
InParanoidi | Q9JHF9 |
OMAi | EVICRGY |
OrthoDBi | 1320082at2759 |
PhylomeDBi | Q9JHF9 |
TreeFami | TF335876 |
Enzyme and pathway databases
Reactomei | R-MMU-140534, Caspase activation via Death Receptors in the presence of ligand R-MMU-166016, Toll Like Receptor 4 (TLR4) Cascade R-MMU-166166, MyD88-independent TLR4 cascade R-MMU-2562578, TRIF-mediated programmed cell death R-MMU-5686938, Regulation of TLR by endogenous ligand R-MMU-936964, Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon R-MMU-937041, IKK complex recruitment mediated by RIP1 R-MMU-937072, TRAF6-mediated induction of TAK1 complex within TLR4 complex R-MMU-975163, IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation |
Miscellaneous databases
BioGRID-ORCSi | 17087, 0 hits in 18 CRISPR screens |
ChiTaRSi | Ly96, mouse |
EvolutionaryTracei | Q9JHF9 |
PROi | PR:Q9JHF9 |
RNActi | Q9JHF9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025779, Expressed in bone marrow macrophage and 183 other tissues |
Genevisiblei | Q9JHF9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR039217, LY96 IPR003172, ML_dom |
PANTHERi | PTHR15218, PTHR15218, 1 hit |
Pfami | View protein in Pfam PF02221, E1_DerP2_DerF2, 1 hit |
SMARTi | View protein in SMART SM00737, ML, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LY96_MOUSE | |
Accessioni | Q9JHF9Primary (citable) accession number: Q9JHF9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references