Hes6

Functionⁱ

Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation.1 Publication

GO - Molecular functionⁱ

DNA-binding transcription factor activity
Source: MGI
Inferred from direct assayⁱ
- 11959828
DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from direct assayⁱ
- 11959828
protein heterodimerization activity
Source: MGI
Inferred from sequence orthologyⁱ
- 11076679
protein homodimerization activity
Source: MGI
Inferred from sequence orthologyⁱ
- 11076679
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
RNA polymerase II regulatory region sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 11959828
sequence-specific DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific double-stranded DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription coregulator activity
Source: MGI
Inferred from direct assayⁱ
- 10851137
transcription corepressor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription factor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation
Source: MGI
Inferred from direct assayⁱ
- 11959828
negative regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 11959828
nervous system development
Source: MGI
Inferred from mutant phenotypeⁱ
- 10851137
Notch signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of neurogenesis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 11959828
somitogenesis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, Repressor
Biological process	Differentiation, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription cofactor HES-6 Alternative name(s): Hairy and enhancer of split 6
Gene namesⁱ	Name:Hes6Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:1859852 Hes6

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127215	1 – 224	Transcription cofactor HES-6Add BLAST		224

Proteomic databases

PaxDbⁱ	Q9JHE6
PRIDEⁱ	Q9JHE6

PTM databases

PhosphoSitePlusⁱ	Q9JHE6

PhosphoSitePlusⁱ

Q9JHE6

Expressionⁱ

Tissue specificityⁱ

Expressed in both undifferentiated and differentiated cells. High levels of expression are observed in several embryonic tissues including the nervous system, muscle and thymus. In the nervous system, initially expressed in the closing neural tube, then in the spinal cord, cranial and dorsal root ganglia, and brain neuroepithelium. Also expressed in epithelial cells of the embryonic respiratory, urinary and digestive systems. In the limb buds, expressed in skeletal muscle and presumptive tendons.2 Publications

Developmental stageⁱ

Expressed in both embryo and adult. Expression in the embryo is detected from E8.5. In the retina, expressed at high levels at postnatal E0. Expression in brain and retina decreases postnatally but still detectable in adult.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000067071 Expressed in 372 organ(s), highest expression level in retina
CleanExⁱ	MM_HES6
Genevisibleⁱ	Q9JHE6 MM

Interactionⁱ

Subunit structureⁱ

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family (By similarity). Interacts with HES1.By similarity1 Publication

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	207731, 5 interactors
STRINGⁱ	10090.ENSMUSP00000084062

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9JHE6
SMRⁱ	Q9JHE6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	25 – 77	bHLHPROSITE-ProRule annotationAdd BLAST		53
Domainⁱ	96 – 129	OrangePROSITE-ProRule annotationAdd BLAST		34

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	221 – 224	WRPW motif		4

Domainⁱ

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

Phylogenomic databases

eggNOGⁱ	KOG4304 Eukaryota ENOG4111F0X LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00700000104168
HOGENOMⁱ	HOG000236346
HOVERGENⁱ	HBG107098
InParanoidⁱ	Q9JHE6
KEGG Orthology (KO) More...KOⁱ	K09087
OMAⁱ	QSVWRPW
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0OZ5
PhylomeDBⁱ	Q9JHE6
TreeFamⁱ	TF351373

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR003650 Orange_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07527 Hairy_orange, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit PS51054 ORANGE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9JHE6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAPSQAPSRD RAGQEDEDRW EARGDRKARK PLVEKKRRAR INESLQELRL 
        60         70         80         90        100
LLAGTEVQAK LENAEVLELT VRRVQGALRG RAREREQLQA EASERFAAGY 
       110        120        130        140        150
IQCMHEVHTF VSTCQAIDAT VSAELLNHLL ESMPLREGSS FQDLLGDSLA 
       160        170        180        190        200
GLPGGSGRSS WPPGGSPESP LSSPPGPGDD LCSDLEEIPE AELNRVPAEG 
       210        220 
PDLVSTSLGS LTAARRAQSV WRPW

Length:224

Mass (Da):24,454

Last modified:October 1, 2000 - v1

Checksum:ⁱF5160A69D913DBE2

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB035178 mRNA Translation: BAA96081.1 AF247040 mRNA Translation: AAF63757.1 AF260236 mRNA Translation: AAK51633.1 AK013443 mRNA Translation: BAB28856.1 AK076135 mRNA Translation: BAC36210.1 AK155340 mRNA Translation: BAE33204.1 BC012897 mRNA Translation: AAH12897.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15163.1
NCBI Reference Sequences More...RefSeqⁱ	NP_062352.1, NM_019479.3
UniGeneⁱ	Mm.280029

Genome annotation databases

Ensemblⁱ	ENSMUST00000086851; ENSMUSP00000084062; ENSMUSG00000067071
GeneIDⁱ	55927
KEGGⁱ	mmu:55927
UCSC genome browser More...UCSCⁱ	uc007cau.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9JHE6	Hairy and enhancer of split 6 (Drosophila), isoform CRA_a		RAT		224	UniRef90_Q9JHE6
Uncharacterized protein		NEOLE		224
UPI000332F71B		JACJA		226
UPI00042AB40C		PERMB		224
UPI0004ED3F36		NANGA		224
+2

Protein	Similar proteins		Species	Score	Length	Source
Q9JHE6	Transcription cofactor HES-6		HUMAN		224	UniRef50_Q9JHE6
Hairy and enhancer of split 6 (Drosophila), isoform CRA_a		RAT		224
Uncharacterized protein		NEOLE		224
transcription cofactor HES-6		DIPOR		225
Uncharacterized protein		PIG		224
+136

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB035178 mRNA Translation: BAA96081.1 AF247040 mRNA Translation: AAF63757.1 AF260236 mRNA Translation: AAK51633.1 AK013443 mRNA Translation: BAB28856.1 AK076135 mRNA Translation: BAC36210.1 AK155340 mRNA Translation: BAE33204.1 BC012897 mRNA Translation: AAH12897.1
CCDSⁱ	CCDS15163.1
RefSeqⁱ	NP_062352.1, NM_019479.3
UniGeneⁱ	Mm.280029

3D structure databases

ProteinModelPortalⁱ	Q9JHE6
SMRⁱ	Q9JHE6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	207731, 5 interactors
STRINGⁱ	10090.ENSMUSP00000084062

PTM databases

PhosphoSitePlusⁱ	Q9JHE6

PhosphoSitePlusⁱ

Q9JHE6

Proteomic databases

PaxDbⁱ	Q9JHE6
PRIDEⁱ	Q9JHE6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000086851; ENSMUSP00000084062; ENSMUSG00000067071
GeneIDⁱ	55927
KEGGⁱ	mmu:55927
UCSCⁱ	uc007cau.1 mouse

Organism-specific databases

CTDⁱ	55502
MGIⁱ	MGI:1859852 Hes6

Phylogenomic databases

eggNOGⁱ	KOG4304 Eukaryota ENOG4111F0X LUCA
GeneTreeⁱ	ENSGT00700000104168
HOGENOMⁱ	HOG000236346
HOVERGENⁱ	HBG107098
InParanoidⁱ	Q9JHE6
KOⁱ	K09087
OMAⁱ	QSVWRPW
OrthoDBⁱ	EOG091G0OZ5
PhylomeDBⁱ	Q9JHE6
TreeFamⁱ	TF351373

Miscellaneous databases

ChiTaRSⁱ	Hes6 mouse
Protein Ontology More...PROⁱ	PR:Q9JHE6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000067071 Expressed in 372 organ(s), highest expression level in retina
CleanExⁱ	MM_HES6
Genevisibleⁱ	Q9JHE6 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR003650 Orange_dom
Pfamⁱ	View protein in Pfam PF07527 Hairy_orange, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit PS51054 ORANGE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HES6_MOUSE
Accessionⁱ	Q9JHE6Primary (citable) accession number: Q9JHE6 Secondary accession number(s): Q3U2D7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
	Last sequence update:	October 1, 2000
	Last modified:	November 7, 2018
	This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9JHE6 (HES6_MOUSE)

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Transcription cofactor HES-6

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ