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UniProtKB - Q9JHE6 (HES6_MOUSE)
Protein
Transcription cofactor HES-6
Gene
Hes6
Organism
Mus musculus (Mouse)
Status
Functioni
Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation.
1 PublicationGO - Molecular functioni
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II-specific DNA-binding transcription factor binding Source: ARUK-UCL
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- anterior/posterior pattern specification Source: GO_Central
- cell differentiation Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: ARUK-UCL
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- nervous system development Source: MGI
- regulation of neurogenesis Source: GO_Central
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Developmental protein, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription cofactor HES-6Alternative name(s): Hairy and enhancer of split 6 |
Gene namesi | Name:Hes6Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859852, Hes6 |
VEuPathDBi | HostDB:ENSMUSG00000067071 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: GO_Central
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127215 | 1 – 224 | Transcription cofactor HES-6Add BLAST | 224 |
Proteomic databases
PaxDbi | Q9JHE6 |
PRIDEi | Q9JHE6 |
ProteomicsDBi | 269659 |
PTM databases
PhosphoSitePlusi | Q9JHE6 |
Expressioni
Tissue specificityi
Expressed in both undifferentiated and differentiated cells. High levels of expression are observed in several embryonic tissues including the nervous system, muscle and thymus. In the nervous system, initially expressed in the closing neural tube, then in the spinal cord, cranial and dorsal root ganglia, and brain neuroepithelium. Also expressed in epithelial cells of the embryonic respiratory, urinary and digestive systems. In the limb buds, expressed in skeletal muscle and presumptive tendons.2 Publications
Developmental stagei
Expressed in both embryo and adult. Expression in the embryo is detected from 8.5 dpc. In the retina, expressed at high levels at postnatal 0 dpc. Expression in brain and retina decreases postnatally but still detectable in adult.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000067071, Expressed in retina and 387 other tissues |
Genevisiblei | Q9JHE6, MM |
Interactioni
Subunit structurei
Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family (By similarity).
Interacts with HES1.
By similarity1 PublicationGO - Molecular functioni
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 207731, 6 interactors |
IntActi | Q9JHE6, 4 interactors |
STRINGi | 10090.ENSMUSP00000084062 |
Miscellaneous databases
RNActi | Q9JHE6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 77 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 96 – 129 | OrangePROSITE-ProRule annotationAdd BLAST | 34 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 31 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 146 – 209 | DisorderedSequence analysisAdd BLAST | 64 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 221 – 224 | WRPW motif | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 29 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.By similarity
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
Phylogenomic databases
eggNOGi | KOG4304, Eukaryota |
GeneTreei | ENSGT00940000161398 |
HOGENOMi | CLU_068550_0_0_1 |
InParanoidi | Q9JHE6 |
OMAi | SAVSKCM |
OrthoDBi | 1483774at2759 |
PhylomeDBi | Q9JHE6 |
TreeFami | TF351373 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR003650, Orange_dom |
Pfami | View protein in Pfam PF07527, Hairy_orange, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS51054, ORANGE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9JHE6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPSQAPSRD RAGQEDEDRW EARGDRKARK PLVEKKRRAR INESLQELRL
60 70 80 90 100
LLAGTEVQAK LENAEVLELT VRRVQGALRG RAREREQLQA EASERFAAGY
110 120 130 140 150
IQCMHEVHTF VSTCQAIDAT VSAELLNHLL ESMPLREGSS FQDLLGDSLA
160 170 180 190 200
GLPGGSGRSS WPPGGSPESP LSSPPGPGDD LCSDLEEIPE AELNRVPAEG
210 220
PDLVSTSLGS LTAARRAQSV WRPW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB035178 mRNA Translation: BAA96081.1 AF247040 mRNA Translation: AAF63757.1 AF260236 mRNA Translation: AAK51633.1 AK013443 mRNA Translation: BAB28856.1 AK076135 mRNA Translation: BAC36210.1 AK155340 mRNA Translation: BAE33204.1 BC012897 mRNA Translation: AAH12897.1 |
CCDSi | CCDS15163.1 |
RefSeqi | NP_062352.1, NM_019479.3 |
Genome annotation databases
Ensembli | ENSMUST00000086851; ENSMUSP00000084062; ENSMUSG00000067071 |
GeneIDi | 55927 |
KEGGi | mmu:55927 |
UCSCi | uc007cau.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB035178 mRNA Translation: BAA96081.1 AF247040 mRNA Translation: AAF63757.1 AF260236 mRNA Translation: AAK51633.1 AK013443 mRNA Translation: BAB28856.1 AK076135 mRNA Translation: BAC36210.1 AK155340 mRNA Translation: BAE33204.1 BC012897 mRNA Translation: AAH12897.1 |
CCDSi | CCDS15163.1 |
RefSeqi | NP_062352.1, NM_019479.3 |
3D structure databases
AlphaFoldDBi | Q9JHE6 |
SMRi | Q9JHE6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207731, 6 interactors |
IntActi | Q9JHE6, 4 interactors |
STRINGi | 10090.ENSMUSP00000084062 |
PTM databases
PhosphoSitePlusi | Q9JHE6 |
Proteomic databases
PaxDbi | Q9JHE6 |
PRIDEi | Q9JHE6 |
ProteomicsDBi | 269659 |
Protocols and materials databases
Antibodypediai | 20277, 289 antibodies from 30 providers |
DNASUi | 55927 |
Genome annotation databases
Ensembli | ENSMUST00000086851; ENSMUSP00000084062; ENSMUSG00000067071 |
GeneIDi | 55927 |
KEGGi | mmu:55927 |
UCSCi | uc007cau.1, mouse |
Organism-specific databases
CTDi | 55502 |
MGIi | MGI:1859852, Hes6 |
VEuPathDBi | HostDB:ENSMUSG00000067071 |
Phylogenomic databases
eggNOGi | KOG4304, Eukaryota |
GeneTreei | ENSGT00940000161398 |
HOGENOMi | CLU_068550_0_0_1 |
InParanoidi | Q9JHE6 |
OMAi | SAVSKCM |
OrthoDBi | 1483774at2759 |
PhylomeDBi | Q9JHE6 |
TreeFami | TF351373 |
Miscellaneous databases
BioGRID-ORCSi | 55927, 4 hits in 77 CRISPR screens |
ChiTaRSi | Hes6, mouse |
PROi | PR:Q9JHE6 |
RNActi | Q9JHE6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000067071, Expressed in retina and 387 other tissues |
Genevisiblei | Q9JHE6, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR003650, Orange_dom |
Pfami | View protein in Pfam PF07527, Hairy_orange, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS51054, ORANGE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HES6_MOUSE | |
Accessioni | Q9JHE6Primary (citable) accession number: Q9JHE6 Secondary accession number(s): Q3U2D7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot