UniProtKB - Q9JHD1 (KAT2B_MOUSE)
Histone acetyltransferase KAT2B
Kat2b
Functioni
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing. Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis. Also acetylates spermidine.
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 551 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- acetyltransferase activity Source: Reactome
- chromatin binding Source: UniProtKB
- cyclin-dependent protein serine/threonine kinase inhibitor activity Source: UniProtKB
- diamine N-acetyltransferase activity Source: UniProtKB-EC
- DNA-binding transcription factor binding Source: Ensembl
- histone acetyltransferase activity Source: MGI
- histone deacetylase binding Source: MGI
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor Source: UniProtKB
- peptide-lysine-N-acetyltransferase activity Source: MGI
- protein kinase binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- transcription coactivator activity Source: MGI
- transcription coregulator activity Source: MGI
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cellular response to insulin stimulus Source: MGI
- chromatin remodeling Source: UniProtKB
- heart development Source: UniProtKB
- histone acetylation Source: MGI
- histone H3 acetylation Source: MGI
- histone H3-K9 acetylation Source: MGI
- internal peptidyl-lysine acetylation Source: UniProtKB
- limb development Source: UniProtKB
- negative regulation of cell population proliferation Source: MGI
- negative regulation of centriole replication Source: UniProtKB
- negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
- negative regulation of rRNA processing Source: UniProtKB
- N-terminal peptidyl-lysine acetylation Source: MGI
- peptidyl-lysine acetylation Source: MGI
- positive regulation of gluconeogenesis Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein acetylation Source: MGI
- regulation of protein ADP-ribosylation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- rhythmic process Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, Acyltransferase, Transferase |
Biological process | Biological rhythms, Cell cycle, Transcription, Transcription regulation |
Enzyme and pathway databases
BRENDAi | 2.3.1.48, 3474 |
Reactomei | R-MMU-2032785, YAP1- and WWTR1 (TAZ)-stimulated gene expression R-MMU-2122947, NOTCH1 Intracellular Domain Regulates Transcription R-MMU-350054, Notch-HLH transcription pathway R-MMU-5250924, B-WICH complex positively regulates rRNA expression R-MMU-5689901, Metalloprotease DUBs R-MMU-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-MMU-8941856, RUNX3 regulates NOTCH signaling R-MMU-9018519, Estrogen-dependent gene expression R-MMU-9617629, Regulation of FOXO transcriptional activity by acetylation |
Names & Taxonomyi
Protein namesi | Recommended name: Histone acetyltransferase KAT2B (EC:2.3.1.48By similarity)Alternative name(s): Histone acetyltransferase PCAF1 Publication Short name: Histone acetylase PCAF1 Publication Lysine acetyltransferase 2BCurated P300/CBP-associated factor1 Publication Short name: P/CAF1 Publication Spermidine acetyltransferase KAT2B (EC:2.3.1.57By similarity) |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1343094, Kat2b |
VEuPathDBi | HostDB:ENSMUSG00000000708 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- centrosome By similarity
Note: Mainly localizes to the nucleus. Also localizes to centrosomes in late G1 and around the G1/S transition, coinciding with the onset of centriole formation.By similarity
Cytoskeleton
- actomyosin Source: MGI
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: MGI
Nucleus
- ATAC complex Source: Ensembl
- histone acetyltransferase complex Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- A band Source: MGI
- I band Source: MGI
- kinetochore Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000322986 | 1 – 813 | Histone acetyltransferase KAT2BAdd BLAST | 813 |
Proteomic databases
jPOSTi | Q9JHD1 |
MaxQBi | Q9JHD1 |
PaxDbi | Q9JHD1 |
PRIDEi | Q9JHD1 |
ProteomicsDBi | 263573 |
PTM databases
iPTMneti | Q9JHD1 |
PhosphoSitePlusi | Q9JHD1 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000000708, Expressed in stria vascularis of cochlear duct and 318 other tissues |
ExpressionAtlasi | Q9JHD1, baseline and differential |
Genevisiblei | Q9JHD1, MM |
Interactioni
Subunit structurei
Interacts with BCAS3.
Interacts with SIRT1.
Interacts with EP300, CREBBP and DDX17.
Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5.
Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity.
Interacts with NFE4.
Interacts with MECOM.
Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2.
Interacts with NFE4.
Interacts with MECOM.
Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity.
Interacts with NPAS2, ARNTL/BMAL1 and CLOCK (By similarity).
Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity.
Interacts with CEBPB (By similarity).
Interacts with NR4A3 (PubMed:12709428).
Interacts with TBX5 (By similarity).
Interacts with PLK4 (By similarity).
Interacts with RB1; this interaction leads to RB1 acetylation (PubMed:20940255).
By similarity4 PublicationsBinary interactionsi
Q9JHD1
With | #Exp. | IntAct |
---|---|---|
Nr2c1 [Q505F1] | 3 | EBI-2325611,EBI-15617004 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: Ensembl
- histone deacetylase binding Source: MGI
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 202042, 23 interactors |
ComplexPortali | CPX-1024, PCAF histone acetylase complex CPX-1029, PCAF-containing ATAC complex CPX-6803, SAGA complex, KAT2B variant |
CORUMi | Q9JHD1 |
DIPi | DIP-29281N |
IntActi | Q9JHD1, 4 interactors |
MINTi | Q9JHD1 |
STRINGi | 10090.ENSMUSP00000000724 |
Miscellaneous databases
RNActi | Q9JHD1, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9JHD1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 484 – 632 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 149 | |
Domaini | 721 – 791 | BromoPROSITE-ProRule annotationAdd BLAST | 71 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 55 | DisorderedSequence analysisAdd BLAST | 55 | |
Regioni | 380 – 423 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 555 – 557 | Acetyl-CoA bindingBy similarity | 3 | |
Regioni | 562 – 568 | Acetyl-CoA bindingBy similarity | 7 | |
Regioni | 593 – 596 | Acetyl-CoA bindingBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 380 – 399 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 409 – 423 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
BromodomainPhylogenomic databases
eggNOGi | KOG1472, Eukaryota |
GeneTreei | ENSGT00940000154995 |
HOGENOMi | CLU_015901_0_0_1 |
InParanoidi | Q9JHD1 |
OMAi | DFAIGYF |
OrthoDBi | 349249at2759 |
PhylomeDBi | Q9JHD1 |
TreeFami | TF105399 |
Family and domain databases
Gene3Di | 1.20.920.10, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR018359, Bromodomain_CS IPR037800, GCN5 IPR016376, GCN5/PCAF IPR000182, GNAT_dom IPR009464, PCAF_N |
PANTHERi | PTHR45750, PTHR45750, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit PF00439, Bromodomain, 1 hit PF06466, PCAF_N, 1 hit |
PIRSFi | PIRSF003048, Histone_acetylase_PCAF, 1 hit |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 1 hit |
SUPFAMi | SSF47370, SSF47370, 1 hit SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS00633, BROMODOMAIN_1, 1 hit PS50014, BROMODOMAIN_2, 1 hit PS51186, GNAT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAEAGGAGSP ALPPAPPHGS PRTLATAAGS SASCGPATAV AAAGTAEGPG
60 70 80 90 100
GGGSARIAVK KAQLRSAPRA KKLEKLGVYS ACKAEESCKC NGWKNPNPSP
110 120 130 140 150
TPPRGDLQQI IVSLTESCRS CSHALAAHVS HLENVSEEEM DRLLGIVLDV
160 170 180 190 200
EYLFTCVHKE EDADTKQVYF YLFKLLRKSI LQRGKPVVEG SLEKKPPFEK
210 220 230 240 250
PSIEQGVNNF VQYKFSHLPS KERQTTIELA KMFLNRINYW HLEAPSQRRL
260 270 280 290 300
RSPNDDISGY KENYTRWLCY CNVPQFCDSL PRYETTKVFG RTLLRSVFTI
310 320 330 340 350
MRRQLLEQAR QEKDKLPLEK RTLILTHFPK FLSMLEEEVY SQNSPIWDQD
360 370 380 390 400
FLSASSRTSP LGIQTVISPP VTGTALFSSN STSHEQINGG RTSPGCRGSS
410 420 430 440 450
GLEANPGEKR KMNNSHAPEE AKRSRVMGDI PVELINEVMS TITDPAGMLG
460 470 480 490 500
PETNFLSAHS ARDEAARLEE RRGVIEFHVV GNSLNQKPNK KILMWLVGLQ
510 520 530 540 550
NVFSHQLPRM PKEYITRLVF DPKHKTLALI KDGRVIGGIC FRMFPSQGFT
560 570 580 590 600
EIVFCAVTSN EQVKGYGTHL MNHLKEYHIK HEILNFLTYA DEYAIGYFKK
610 620 630 640 650
QGFSKEIKIP KTKYVGYIKD YEGATLMGCE LNPQIPYTEF SVIIKKQKEI
660 670 680 690 700
IKKLIERKQA QIRKVYPGLS CFKDGVRQIP IESIPGIRET GWKPSGKEKS
710 720 730 740 750
KEPKDPEQLY STLKNILQQV KNHPNAWPFM EPVKRTEAPG YYEVIRFPMD
760 770 780 790 800
LKTMSERLRN RYYVSKKLFM ADLQRVFTNC KEYNPPESEY YKCASILEKF
810
FFSKIKEAGL IDK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q1F7 | E9Q1F7_MOUSE | Histone acetyltransferase KAT2B | Kat2b | 211 | Annotation score: | ||
F6ZCH1 | F6ZCH1_MOUSE | Histone acetyltransferase KAT2B | Kat2b | 66 | Annotation score: | ||
F6SIC5 | F6SIC5_MOUSE | Histone acetyltransferase KAT2B | Kat2b | 118 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 293 – 294 | LL → FV in AAF70498 (PubMed:9742083).Curated | 2 | |
Sequence conflicti | 319 | E → G in BAE33658 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 459 | H → L in AAF70498 (PubMed:9742083).Curated | 1 | |
Sequence conflicti | 548 | G → A in AAF70498 (PubMed:9742083).Curated | 1 | |
Sequence conflicti | 554 | F → L in AAF70498 (PubMed:9742083).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF254442 mRNA Translation: AAF70498.1 AK156290 mRNA Translation: BAE33658.1 BC082581 mRNA Translation: AAH82581.1 BC145896 mRNA Translation: AAI45897.1 |
CCDSi | CCDS28880.1 |
RefSeqi | NP_064389.2, NM_020005.4 |
Genome annotation databases
Ensembli | ENSMUST00000000724; ENSMUSP00000000724; ENSMUSG00000000708 |
GeneIDi | 18519 |
KEGGi | mmu:18519 |
UCSCi | uc008czp.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF254442 mRNA Translation: AAF70498.1 AK156290 mRNA Translation: BAE33658.1 BC082581 mRNA Translation: AAH82581.1 BC145896 mRNA Translation: AAI45897.1 |
CCDSi | CCDS28880.1 |
RefSeqi | NP_064389.2, NM_020005.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ML0 | X-ray | 1.64 | A | 705-813 | [»] | |
SMRi | Q9JHD1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202042, 23 interactors |
ComplexPortali | CPX-1024, PCAF histone acetylase complex CPX-1029, PCAF-containing ATAC complex CPX-6803, SAGA complex, KAT2B variant |
CORUMi | Q9JHD1 |
DIPi | DIP-29281N |
IntActi | Q9JHD1, 4 interactors |
MINTi | Q9JHD1 |
STRINGi | 10090.ENSMUSP00000000724 |
PTM databases
iPTMneti | Q9JHD1 |
PhosphoSitePlusi | Q9JHD1 |
Proteomic databases
jPOSTi | Q9JHD1 |
MaxQBi | Q9JHD1 |
PaxDbi | Q9JHD1 |
PRIDEi | Q9JHD1 |
ProteomicsDBi | 263573 |
Protocols and materials databases
Antibodypediai | 3865, 453 antibodies from 39 providers |
DNASUi | 18519 |
Genome annotation databases
Ensembli | ENSMUST00000000724; ENSMUSP00000000724; ENSMUSG00000000708 |
GeneIDi | 18519 |
KEGGi | mmu:18519 |
UCSCi | uc008czp.2, mouse |
Organism-specific databases
CTDi | 8850 |
MGIi | MGI:1343094, Kat2b |
VEuPathDBi | HostDB:ENSMUSG00000000708 |
Phylogenomic databases
eggNOGi | KOG1472, Eukaryota |
GeneTreei | ENSGT00940000154995 |
HOGENOMi | CLU_015901_0_0_1 |
InParanoidi | Q9JHD1 |
OMAi | DFAIGYF |
OrthoDBi | 349249at2759 |
PhylomeDBi | Q9JHD1 |
TreeFami | TF105399 |
Enzyme and pathway databases
BRENDAi | 2.3.1.48, 3474 |
Reactomei | R-MMU-2032785, YAP1- and WWTR1 (TAZ)-stimulated gene expression R-MMU-2122947, NOTCH1 Intracellular Domain Regulates Transcription R-MMU-350054, Notch-HLH transcription pathway R-MMU-5250924, B-WICH complex positively regulates rRNA expression R-MMU-5689901, Metalloprotease DUBs R-MMU-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-MMU-8941856, RUNX3 regulates NOTCH signaling R-MMU-9018519, Estrogen-dependent gene expression R-MMU-9617629, Regulation of FOXO transcriptional activity by acetylation |
Miscellaneous databases
BioGRID-ORCSi | 18519, 0 hits in 66 CRISPR screens |
ChiTaRSi | Kat2b, mouse |
PROi | PR:Q9JHD1 |
RNActi | Q9JHD1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000708, Expressed in stria vascularis of cochlear duct and 318 other tissues |
ExpressionAtlasi | Q9JHD1, baseline and differential |
Genevisiblei | Q9JHD1, MM |
Family and domain databases
Gene3Di | 1.20.920.10, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR018359, Bromodomain_CS IPR037800, GCN5 IPR016376, GCN5/PCAF IPR000182, GNAT_dom IPR009464, PCAF_N |
PANTHERi | PTHR45750, PTHR45750, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit PF00439, Bromodomain, 1 hit PF06466, PCAF_N, 1 hit |
PIRSFi | PIRSF003048, Histone_acetylase_PCAF, 1 hit |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 1 hit |
SUPFAMi | SSF47370, SSF47370, 1 hit SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS00633, BROMODOMAIN_1, 1 hit PS50014, BROMODOMAIN_2, 1 hit PS51186, GNAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KAT2B_MOUSE | |
Accessioni | Q9JHD1Primary (citable) accession number: Q9JHD1 Secondary accession number(s): Q3U142, Q640M9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | March 18, 2008 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families