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Protein

Histone acetyltransferase KAT2B

Gene

Kat2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, PLK4 and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4.By similarity

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei551Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Acyltransferase, Transferase
Biological processBiological rhythms, Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2032785 YAP1- and WWTR1 (TAZ)-stimulated gene expression
R-MMU-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-MMU-350054 Notch-HLH transcription pathway
R-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-5689901 Metalloprotease DUBs
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8941856 RUNX3 regulates NOTCH signaling
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase KAT2B (EC:2.3.1.48By similarity)
Alternative name(s):
Histone acetyltransferase PCAF1 Publication
Short name:
Histone acetylase PCAF1 Publication
Lysine acetyltransferase 2BCurated
P300/CBP-associated factor1 Publication
Short name:
P/CAF1 Publication
Gene namesi
Name:Kat2bImported
Synonyms:Pcaf1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1343094 Kat2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype (PubMed:11017084).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003229861 – 813Histone acetyltransferase KAT2BAdd BLAST813

Proteomic databases

MaxQBiQ9JHD1
PaxDbiQ9JHD1
PRIDEiQ9JHD1

PTM databases

iPTMnetiQ9JHD1
PhosphoSitePlusiQ9JHD1

Expressioni

Developmental stagei

Expression is low during embryogenesis and becomes up-regulated in some adult tissues including heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000000708
ExpressionAtlasiQ9JHD1 baseline and differential
GenevisibleiQ9JHD1 MM

Interactioni

Subunit structurei

Interacts with BCAS3. Interacts with SIRT1. Interacts with EP300, CREBBP and DDX17. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Interacts with NPAS2, ARNTL/BMAL1 and CLOCK (By similarity). Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity. Interacts with CEBPB (By similarity). Interacts with NR4A3 (PubMed:12709428). Interacts with TBX5 (By similarity). Interacts with PLK4 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nr2c1Q505F13EBI-2325611,EBI-15617004

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202042, 20 interactors
ComplexPortaliCPX-1024 PCAF histone acetylase complex
CPX-1029 PCAF-containing ATAC complex
DIPiDIP-29281N
IntActiQ9JHD1, 3 interactors
MINTiQ9JHD1
STRINGi10090.ENSMUSP00000000724

Structurei

Secondary structure

1813
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi707 – 722Combined sources16
Helixi727 – 729Combined sources3
Turni735 – 737Combined sources3
Helixi741 – 744Combined sources4
Helixi751 – 759Combined sources9
Helixi766 – 783Combined sources18
Helixi789 – 807Combined sources19

3D structure databases

ProteinModelPortaliQ9JHD1
SMRiQ9JHD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini484 – 632N-acetyltransferasePROSITE-ProRule annotationAdd BLAST149
Domaini721 – 791BromoPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni555 – 557Acetyl-CoA bindingBy similarity3
Regioni562 – 568Acetyl-CoA bindingBy similarity7
Regioni593 – 596Acetyl-CoA bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi48 – 53Poly-Gly6

Sequence similaritiesi

Keywords - Domaini

Bromodomain

Phylogenomic databases

eggNOGiKOG1472 Eukaryota
COG5076 LUCA
GeneTreeiENSGT00920000149009
HOGENOMiHOG000007151
InParanoidiQ9JHD1
KOiK06062
OMAiQVIRFPM
OrthoDBiEOG091G03ZO
PhylomeDBiQ9JHD1
TreeFamiTF105399

Family and domain databases

Gene3Di1.20.920.10, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR037800 GCN5
IPR016376 GCN5/PCAF
IPR000182 GNAT_dom
IPR009464 PCAF_N
PANTHERiPTHR22880:SF124 PTHR22880:SF124, 1 hit
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
PF00439 Bromodomain, 1 hit
PF06466 PCAF_N, 1 hit
PIRSFiPIRSF003048 Histone_acetylase_PCAF, 1 hit
PRINTSiPR00503 BROMODOMAIN
SMARTiView protein in SMART
SM00297 BROMO, 1 hit
SUPFAMiSSF47370 SSF47370, 1 hit
SSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS00633 BROMODOMAIN_1, 1 hit
PS50014 BROMODOMAIN_2, 1 hit
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JHD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAGGAGSP ALPPAPPHGS PRTLATAAGS SASCGPATAV AAAGTAEGPG
60 70 80 90 100
GGGSARIAVK KAQLRSAPRA KKLEKLGVYS ACKAEESCKC NGWKNPNPSP
110 120 130 140 150
TPPRGDLQQI IVSLTESCRS CSHALAAHVS HLENVSEEEM DRLLGIVLDV
160 170 180 190 200
EYLFTCVHKE EDADTKQVYF YLFKLLRKSI LQRGKPVVEG SLEKKPPFEK
210 220 230 240 250
PSIEQGVNNF VQYKFSHLPS KERQTTIELA KMFLNRINYW HLEAPSQRRL
260 270 280 290 300
RSPNDDISGY KENYTRWLCY CNVPQFCDSL PRYETTKVFG RTLLRSVFTI
310 320 330 340 350
MRRQLLEQAR QEKDKLPLEK RTLILTHFPK FLSMLEEEVY SQNSPIWDQD
360 370 380 390 400
FLSASSRTSP LGIQTVISPP VTGTALFSSN STSHEQINGG RTSPGCRGSS
410 420 430 440 450
GLEANPGEKR KMNNSHAPEE AKRSRVMGDI PVELINEVMS TITDPAGMLG
460 470 480 490 500
PETNFLSAHS ARDEAARLEE RRGVIEFHVV GNSLNQKPNK KILMWLVGLQ
510 520 530 540 550
NVFSHQLPRM PKEYITRLVF DPKHKTLALI KDGRVIGGIC FRMFPSQGFT
560 570 580 590 600
EIVFCAVTSN EQVKGYGTHL MNHLKEYHIK HEILNFLTYA DEYAIGYFKK
610 620 630 640 650
QGFSKEIKIP KTKYVGYIKD YEGATLMGCE LNPQIPYTEF SVIIKKQKEI
660 670 680 690 700
IKKLIERKQA QIRKVYPGLS CFKDGVRQIP IESIPGIRET GWKPSGKEKS
710 720 730 740 750
KEPKDPEQLY STLKNILQQV KNHPNAWPFM EPVKRTEAPG YYEVIRFPMD
760 770 780 790 800
LKTMSERLRN RYYVSKKLFM ADLQRVFTNC KEYNPPESEY YKCASILEKF
810
FFSKIKEAGL IDK
Length:813
Mass (Da):91,769
Last modified:March 18, 2008 - v2
Checksum:i38E52F326794F523
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti293 – 294LL → FV in AAF70498 (PubMed:9742083).Curated2
Sequence conflicti319E → G in BAE33658 (PubMed:16141072).Curated1
Sequence conflicti459H → L in AAF70498 (PubMed:9742083).Curated1
Sequence conflicti548G → A in AAF70498 (PubMed:9742083).Curated1
Sequence conflicti554F → L in AAF70498 (PubMed:9742083).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254442 mRNA Translation: AAF70498.1
AK156290 mRNA Translation: BAE33658.1
BC082581 mRNA Translation: AAH82581.1
BC145896 mRNA Translation: AAI45897.1
CCDSiCCDS28880.1
RefSeqiNP_064389.2, NM_020005.4
UniGeneiMm.255025

Genome annotation databases

EnsembliENSMUST00000000724; ENSMUSP00000000724; ENSMUSG00000000708
GeneIDi18519
KEGGimmu:18519
UCSCiuc008czp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiKAT2B_MOUSE
AccessioniPrimary (citable) accession number: Q9JHD1
Secondary accession number(s): Q3U142, Q640M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: July 18, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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