UniProtKB - Q9JH69 (NS1AB_TASV1)
Non-structural polyprotein 1AB
ORF1
Functioni
Contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA.
Protein covalently attached to the 5' extremity of the genomic and subgenomic RNAs.
By similarityCatalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 550 | Charge relay system; for serine protease activityBy similarity | 1 | |
Active sitei | 582 | Charge relay system; for serine protease activityBy similarity | 1 | |
Active sitei | 647 | Charge relay system; for serine protease activityBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type peptidase activity Source: UniProtKB-KW
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase |
Biological process | Viral RNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Non-structural polyprotein 1ABCleaved into the following 6 chains: |
Gene namesi | Name:ORF1 |
Organismi | Turkey astrovirus 1 (TAstV-1) |
Taxonomic identifieri | 364370 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Stelpaviricetes › Stellavirales › Astroviridae › Avastrovirus › |
Virus hosti | Meleagris gallopavo (Wild turkey) [TaxID: 9103] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 195 – 215 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 329 – 348 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 353 – 373 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 375 – 395 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 397 – 417 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 426 – 446 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 450 – 470 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000327307 | 1 – 1611 | Non-structural polyprotein 1ABAdd BLAST | 1611 | |
ChainiPRO_0000327308 | 1 – 168 | Protein p19Sequence analysisAdd BLAST | 168 | |
ChainiPRO_0000327309 | 169 – 508 | Transmembrane protein 1ASequence analysisAdd BLAST | 340 | |
ChainiPRO_0000327310 | 509 – 795 | Serine protease p27Sequence analysisAdd BLAST | 287 | |
ChainiPRO_0000419592 | 796 – 915 | VPgSequence analysisAdd BLAST | 120 | |
ChainiPRO_0000327311 | 916 – 1027 | Protein p20Sequence analysisAdd BLAST | 112 | |
ChainiPRO_0000327312 | 1028 – 1611 | RNA-directed RNA polymerase p57Sequence analysisAdd BLAST | 584 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 168 – 169 | CleavageSequence analysis | 2 | |
Sitei | 508 – 509 | CleavageSequence analysis | 2 | |
Sitei | 795 – 796 | CleavageSequence analysis | 2 | |
Sitei | 915 – 916 | CleavageSequence analysis | 2 | |
Sitei | 1027 – 1028 | CleavageSequence analysis | 2 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1352 – 1486 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 135 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 124 – 187 | Sequence analysisAdd BLAST | 64 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 2.40.10.10, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR045836, Astro_VPg IPR043502, DNA/RNA_pol_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF19416, Astro_VPg, 1 hit PF00680, RdRP_1, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAAASALG ASAPKALAPA DGPIVAGLDK LVNLEGVHDL FEAMRGAYGE
60 70 80 90 100
DPAWKGLMSC DVVYLKDITT AIGVKDTSVG IFRKFSDGCS WCPTGAECFL
110 120 130 140 150
SMKDLAYMKA QSAKAQRLTA SLATTSNLIA RAMRAESELK RARDEERKVD
160 170 180 190 200
ARYKDILEHS LAARKALQKE LDETRERELH LLKELGKRSS IRTKAFSFFD
210 220 230 240 250
WLFMAVVFFL FLHYTSAECV KPDFGCLVVN SNLPVPSLTF HDVMARCYNT
260 270 280 290 300
FGNIVLSSQI DAARLREECE QSANKFLGTH IGDPAHKVWC ENRLETLIPV
310 320 330 340 350
ECDSSEFLEI FTSNLNAFMV SVSQFYKTIS YYKLDALVTF AFSAALATNK
360 370 380 390 400
LKMVMVLPLL LVALYLNVPP ITVTIASVIF QPLILPFVGF QLVFPNFLPY
410 420 430 440 450
NLFVAWVWMV CQAFFSSDGV KLLVSVSTAL VQVVFLAVWS ISVIVLQQLS
460 470 480 490 500
IPMVAQILLF VATLTVSVGV KFANSTITVV HPDGNTEKVS RVTLVRQSMA
510 520 530 540 550
KRISQIKQSL TIRGVIPSGP NRFDSIVVVE GQGGSGVGWR FMNSIFTAGH
560 570 580 590 600
VVQGSKFVTI KSESTQVKVK VKRVIDLFEC VDTLVEIPLT KEFQHIKPLR
610 620 630 640 650
LAKKVEDSYL QLCAFKPDMV EKASYQGWCT IDSGFIFNSF NTQFGNSGAP
660 670 680 690 700
YVDSDGRLVG MHLGSQGVIS QGVVLVDTLK TQFLAQQSQI DDQLMERIIE
710 720 730 740 750
GTKVSHAAIL TELDRMRTKV EEVALVSARV NQLESQLKDL YEFSSNSIKC
760 770 780 790 800
LSDDIEKMVC AQLFDEINLQ SVMEKISALP PTEKLAKLVE VFVEQKKKGK
810 820 830 840 850
TKRTARGGKH ALGKKYLSKA HFSRMRMLTE EEYNKMVEDG FSPDEIKEVV
860 870 880 890 900
DQLREQAWQN YLIDNDIGED DDLDWYDDML EDERLNEEID RRVEAALEDR
910 920 930 940 950
GELAYQKIRR TFVDQALIHL ITLKKGNWQT TKVECQPERE EAYKEQFQKA
960 970 980 990 1000
VKQEDLTEGT SYAIYSAGDA TILIENKEID HTEIKPVTTG AKTVQEYPKD
1010 1020 1030 1040 1050
ARTTVATFDD NKKDIVKTKR TTEIVLEQRK KTCRTCGETR PHNHKMCRDR
1060 1070 1080 1090 1100
HTRRFCFWCG VVHSDVEGHS RDLKCPKCSA GFANLREMEQ HAVTTCSKKL
1110 1120 1130 1140 1150
DSHPEPSRVF QPLDFGLGIF DWRFDLQPIR HHVAVPMNVE VLGYIPVDRL
1160 1170 1180 1190 1200
VERRNVITDP LLKLVEPWRQ ETYGPAVWTI KAYNKMFEKF FYSEPLEFAQ
1210 1220 1230 1240 1250
LDSSILNLAD SYCLQEHDYM SGSQIVPITS TEKNLDSTPG YPKFKVFSTE
1260 1270 1280 1290 1300
REYLSTCGWD EYKTVWQVGP REKPLWWCFL KTEVLKLAKI EQDDIRMILC
1310 1320 1330 1340 1350
TDPVFTRIGA AFEQHQNSLM KLETENHHAQ VGWSPFFGGI HRRATRLYGE
1360 1370 1380 1390 1400
HRYYVELDWT RFDGTIPPEL FRRIKLMRFF LLDPKYKTPE NRDRYNWYVE
1410 1420 1430 1440 1450
NLIDKVVLLP TGEVCKIYGG NPSGQFSTTV DNNFVNVWLT VFELAYLFYK
1460 1470 1480 1490 1500
EHNRLPTICE IKKHTDWICY GDDRLLAVDK RFINSYDTAA VIAMYKDVFG
1510 1520 1530 1540 1550
MWVKPDNIKV FPSLEGVSFC GMVWTKRKGQ YVGKPNVDKI LSTLSDPVSR
1560 1570 1580 1590 1600
LPDIQSLWGK LVSLRLLCEN ESDEVVDYLD KQIESVSRHA KEAGIALPKI
1610
GPDFYAEIWI D
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y15936 Genomic RNA Translation: CAB95006.3 Sequence problems. |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingCross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y15936 Genomic RNA Translation: CAB95006.3 Sequence problems. |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family and domain databases
Gene3Di | 2.40.10.10, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR045836, Astro_VPg IPR043502, DNA/RNA_pol_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF19416, Astro_VPg, 1 hit PF00680, RdRP_1, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50507, RDRP_SSRNA_POS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NS1AB_TASV1 | |
Accessioni | Q9JH69Primary (citable) accession number: Q9JH69 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | April 8, 2008 | |
Last modified: | February 23, 2022 | |
This is version 82 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families