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Entry version 77 (11 Dec 2019)
Sequence version 2 (08 Apr 2008)
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Protein

Non-structural polyprotein 1AB

Gene

ORF1

Organism
Ovine astrovirus 1 (OAstV-1)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA.
protein covalently attached to the 5' extremity of the genomic and subgenomic RNAs.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei452Charge relay system; for serine protease activityBy similarity1
Active sitei481Charge relay system; for serine protease activityBy similarity1
Active sitei544Charge relay system; for serine protease activityBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Non-structural polyprotein 1AB
Cleaved into the following 6 chains:
Serine protease p27 (EC:3.4.21.-)
Short name:
p27
RNA-directed RNA polymerase p57 (EC:2.7.7.48)
Short name:
p57
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ORF1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiOvine astrovirus 1 (OAstV-1)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1239577 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaAstroviridaeMamastrovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiOvis aries (Sheep) [TaxID: 9940]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000007786 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Transmembranei231 – 251HelicalSequence analysisAdd BLAST21
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Transmembranei304 – 324HelicalSequence analysisAdd BLAST21
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003273011 – 1351Non-structural polyprotein 1ABAdd BLAST1351
ChainiPRO_00003273021 – 172Protein p19Sequence analysisAdd BLAST172
ChainiPRO_0000327303173 – 407Transmembrane protein 1ASequence analysisAdd BLAST235
ChainiPRO_0000327304408 – 640Serine protease p27Sequence analysisAdd BLAST233
ChainiPRO_0000419591641 – 721VPgSequence analysisAdd BLAST81
ChainiPRO_0000327305722 – 826Protein p20Sequence analysisAdd BLAST105
ChainiPRO_0000327306827 – 1351RNA-directed RNA polymerase p57Sequence analysisAdd BLAST525

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Cleaved presumably by viral and host proteases.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei172 – 173CleavageSequence analysis2
Sitei407 – 408CleavageSequence analysis2
Sitei640 – 641CleavageSequence analysis2
Sitei721 – 722CleavageSequence analysis2
Sitei826 – 827CleavageSequence analysis2

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q9JH66

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1085 – 1217RdRp catalyticPROSITE-ProRule annotationAdd BLAST133

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili105 – 144Sequence analysisAdd BLAST40
Coiled coili579 – 635Sequence analysisAdd BLAST57

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

Database of Orthologous Groups

More...
OrthoDBi
242at10239

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001205 RNA-dir_pol_C
IPR007094 RNA-dir_pol_PSvirus

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00680 RdRP_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50494 SSF50494, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50507 RDRP_SSRNA_POS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by ribosomal frameshifting. AlignAdd to basket
Isoform nsp1ab (identifier: Q9JH66-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNVYDKVLQF GSKKARARGM ALNKLSRNRL EDIYAGSGPL VFGFGPIDMV
60 70 80 90 100
DPGSLNPSIK TLDTVYVAAV QPDNQYVVHH FVPGRNEWVE TDASTHQPTA
110 120 130 140 150
LVGVLVQDHK AKTREVEDLK SQLSQLRMEH EILRHEYERL KLKSPIVKPF
160 170 180 190 200
KPLKVLLFSL LLGLLFAGVT NGARTGTCYA YDEEKDTCLY WEWKDSREVA
210 220 230 240 250
WYDSYVTEAL AIYNRACVYV RSKEFMTYLS LVFQTVFNWY FCATALAVYY
260 270 280 290 300
MARAENPIVM FVTLALATLS QFQLLAVAVL PLLDFSATMG LWLSMVVFYM
310 320 330 340 350
SQQISILVSF CVLVLSVMIG TFMADSEYAM MIKGHAVVFA IVCYSHVAMI
360 370 380 390 400
LNIPPWVVSL TMVCYRLWRV CFVFPAERLE IRSADGKVLH TVPTHPNWTA
410 420 430 440 450
KVTRFVQSLR KGLRTSVAPT ARIVPDGIAI VEAREGVGTC FRVKNNLVTS
460 470 480 490 500
KHVVGSDDAV KIRWGAQEDM ARVTYRHPTK DIALMALPTN LQTMPAYKFA
510 520 530 540 550
KAITDGPIVM TAFDEANLLL VAVTEGVRVE DHMTYSVATR NGMSGAPITT
560 570 580 590 600
VDGRVIAVHQ TNTGFTGGAV IFVPEDIPEV RKISKREQEL EDRVKQLEGM
610 620 630 640 650
LNMDQAYVDS NLIVDLVREA VQREMKVLRT ELANLGGFSQ KKKGKNKSTK
660 670 680 690 700
RKRKAVWTEE EYKAMLEKGF TRDQLRIMAD AIRDQYYDDE DEQSEEEAGY
710 720 730 740 750
PDWSDPGDST DIENEWFGYE QSWKELEPAK SGVVVNTLPK DLVFKYSLDN
760 770 780 790 800
YPISKQDIQA VAKELKIYEK AISDIISTSV STDGKWKDDV DAQKILQELD
810 820 830 840 850
GLWWGINHTL WEHGLMPFTQ RRKRVQQPKK LQRGPEDPGP EECKLDYWEQ
860 870 880 890 900
LVEPSKEKFL VPPEYPLLGV VPLDRPISDF DAPVDNLLAL LPEPESPDLG
910 920 930 940 950
FEPAVWGPEA YVKSFEKFDF ADPDPNIEKN YPREWAFANL VLHREFDFLA
960 970 980 990 1000
DSVVKDITAT SKNSESTPGF PKTYWWKTEA EYLAKRGYAD YVSEWNRIRG
1010 1020 1030 1040 1050
GARPNVLWYL FLKKEILKST KVRDADIRQI ICSDPIFARI GCCFEEDQNE
1060 1070 1080 1090 1100
RMKRRTKTRM PQCGWSPFFG GFNDRIQRLV AKGNPYWIEF DWTRYDGTIP
1110 1120 1130 1140 1150
SQIFKHIKNF RFSMLAKEYQ TPELRNMYHW YVDNILRRYV CMPSGEITIQ
1160 1170 1180 1190 1200
HKGNPSGQVS TTMDNNLVNV FLQAFEYAYL HPEKSMDELR KDWESYDSLI
1210 1220 1230 1240 1250
YGDDRLTTSP SVPNDYVTRV VAMYKDIFGM WVKPEKVKVS HSPVGLSFCG
1260 1270 1280 1290 1300
FVITHQDGQY LPVPAEEAKL LASLLRPTKK LENMDALYGK LLCYRILNHN
1310 1320 1330 1340 1350
LPNDNKFRNY ILVALEVMAR HYSSRGEEPP FYVTESMLDK LWRGGPKFDY

G
Note: Generated by a ribosomal frameshift at position 830.
Length:1,351
Mass (Da):154,740
Last modified:April 8, 2008 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iFB0A954B26292C54
GO
Isoform nsp1a (identifier: Q9JH67-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9JH67.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:844
Mass (Da):95,455
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Y15937 Genomic RNA Translation: CAB95003.1 Sequence problems.

NCBI Reference Sequences

More...
RefSeqi
NP_059945.2, NC_002469.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1449587

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vg:1449587

Keywords - Coding sequence diversityi

Ribosomal frameshifting

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15937 Genomic RNA Translation: CAB95003.1 Sequence problems.
RefSeqiNP_059945.2, NC_002469.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Proteomic databases

PRIDEiQ9JH66

Genome annotation databases

GeneIDi1449587
KEGGivg:1449587

Phylogenomic databases

OrthoDBi242at10239

Family and domain databases

InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001205 RNA-dir_pol_C
IPR007094 RNA-dir_pol_PSvirus
PfamiView protein in Pfam
PF00680 RdRP_1, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50507 RDRP_SSRNA_POS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNS1AB_OASV1
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9JH66
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: December 11, 2019
This is version 77 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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