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Protein

Envelopment polyprotein

Gene

GP

Organism
Tomato spotted wilt virus (strain Regular2A) (TSWV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Glycoprotein N: Together with Glycoprotein C are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity
Glycoprotein C: Together with Glycoprotein N are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processFusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelopment polyprotein
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Glycoprotein NBy similarity
Short name:
Gn
Alternative name(s):
Glycoprotein G1
Glycoprotein CBy similarity
Short name:
Gc
Alternative name(s):
Glycoprotein G2
Gene namesi
Name:GP
OrganismiTomato spotted wilt virus (strain Regular2A) (TSWV)
Taxonomic identifieri267287 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesBunyaviralesTospoviridaeOrthotospovirus
Virus hostiFrankliniella occidentalis (Western flower thrips) [TaxID: 133901]
Scirtothrips dorsalis [TaxID: 163899]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Thrips tabaci [TaxID: 161014]

Subcellular locationi

Glycoprotein N :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
  • Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity
  • Note: Glycoprotein C alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of Glycoprotein C and Glycoprotein N results in efficient transport of Glycoprotein C to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs.By similarity
Glycoprotein C :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
  • Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 345LumenalSequence analysisAdd BLAST310
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 484CytoplasmicSequence analysisAdd BLAST118
Topological domaini485 – 1067LumenalSequence analysisAdd BLAST583
Transmembranei1068 – 1088HelicalSequence analysisAdd BLAST21
Topological domaini1089 – 1135CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000003686536 – 1135Envelopment polyproteinAdd BLAST1100
ChainiPRO_000003686636 – 484Glycoprotein NBy similarityAdd BLAST449
ChainiPRO_0000036867485 – 1135Glycoprotein CBy similarityAdd BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi116N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi210N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi340N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi588N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi605N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi980N-linked (GlcNAc...) asparagine; by hostSequence analysis1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.By similarity
Glycosylated. Glycosylation is essential for proper subcellular location (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei484 – 485Cleavage; by host signal peptidaseBy similarity2

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9IKB5

Interactioni

Subunit structurei

Glycoprotein N: Interacts with Glycoprotein C and nucleoprotein. Glycoprotein C: Interacts with Glycoprotein N and nucleoprotein.By similarity

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 43Cell attachment siteSequence analysis3

Domaini

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiView protein in InterPro
IPR005167 Bunya_G1
IPR014414 M_poly_TospoV
PfamiView protein in Pfam
PF03557 Bunya_G1, 1 hit
PIRSFiPIRSF003960 M_poly_TospoV, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9IKB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILKLLELV VKVSLFTIAL SSVLLAFLIF RATDAKVEII RGDHPEVYDD
60 70 80 90 100
SAENEVPTAA SIQRKAILET LTNLMLESQT PGTRQIREEE STIPIFAEST
110 120 130 140 150
TQKIISVSDL PNNCLNASSL KCEIKGISTY NVYYQVENND VIYSCVSDSA
160 170 180 190 200
EGLEKCDNSL NLPKRFSKVP VIPITKLDNK RHFSVGTKFF ISESLTQDNY
210 220 230 240 250
PITYNSYPTN GTVSLQTVKL SGDCKITKSN FANPYTVSIT SPEKIMGYLI
260 270 280 290 300
KKPGENVEHK VISFSGSASI TFTEEMLDGE HNLLCGDKSA KIPKTNKRVR
310 320 330 340 350
DCIIKYSKSI YKQTACINFS WIRLILIALL IYFPIRWLVN KTTKPLFLWY
360 370 380 390 400
DLIGLITYPI LLLINCLWKY FPFKCSNRGN LCIITHKCTK LCICNKSKAS
410 420 430 440 450
KEHSLECPIL SKETDHGYNK HKWTSMEWFH LIVNTKLSFS LLKFVTEILI
460 470 480 490 500
GLIILSQMPM SMAQTTQCLS GCFYVPGCPF LVTSKFEKCP ERDQCYCNVK
510 520 530 540 550
EDKIIESIFG TNIIIEGPND CIENQNCAAN PSIDNLIKCR LGCEYLDLFR
560 570 580 590 600
NKPLYNGFSD YTGSSLGLTS IGLYEAKRLR NGIIDSYNRT DKISGMIAGD
610 620 630 640 650
SLNKNETSIP ENILPRQSLI FDSVVDGKYR YMIEQSLLGG GGTIFMLNDK
660 670 680 690 700
TSETAKKFVI YIKSVGIHYE VSEKYTTAPI QSTHTDFYST CTGNCDTCRK
710 720 730 740 750
NQALTGFQDF CITPTSYWGC EEAWCFAINE GATCGFCRNI YDMDKSYRIY
760 770 780 790 800
SVLKSTIVAD VCISGILGGQ CSRITEEVPY ENALFQADIQ ADLHNDGITI
810 820 830 840 850
GELIAHGPDS HIYSGNIANL NDPVKMFGHP QLTRDGVPIF TKKTLEGDDM
860 870 880 890 900
SWDCAAIGKK SITIKTCGYD TYRFRSGLEQ ISDIPVSFKD FSSFFLEKAF
910 920 930 940 950
SLGKLKIVVD LPSDLFKVAP KKPSITSTSL NCNGCLLCGQ GLSCILEFFS
960 970 980 990 1000
DLTFSTAISI DACSLSTYQL AVKKGSNKYN ITMFCSANPD KKKMTLYPEG
1010 1020 1030 1040 1050
NPDISVEVLV NNVIVEEPEN IIDQNDEYAH EEQQYNSDSS AWGFWDYIKS
1060 1070 1080 1090 1100
PFNFIASYFG SFFDTIRVIL LIAFIFLVIY FCSILTTICK GYVKNESYKS
1110 1120 1130
RSKIEDDDDS EIKAPMLMKD TMTRRRPPMD FSHLV
Length:1,135
Mass (Da):127,641
Last modified:October 1, 2000 - v1
Checksum:i4FA0B9433BBCB197
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208498 Genomic RNA Translation: AAF80981.1

Similar proteinsi

Entry informationi

Entry nameiGP_TSWVR
AccessioniPrimary (citable) accession number: Q9IKB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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