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Protein

Hairy/enhancer-of-split related with YRPW motif protein 1

Gene

hey1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Downstream effector of Notch signaling. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Acts as a suppressor of neurogenesis by antagonizing proneural gene function. Functions during floorplate development. Plays a role in pronephros formation in the inhibition of distal tubule and duct cell fates and the promotion of glomus and proximal tubule formation.By similarity3 Publications

GO - Molecular functioni

  • bHLH transcription factor binding Source: UniProtKB
  • DNA binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • floor plate development Source: UniProtKB
  • glomus development Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • Notch signaling pathway Source: UniProtKB
  • pronephros development Source: UniProtKB
  • proximal/distal pattern formation involved in pronephric nephron development Source: UniProtKB
  • specification of proximal tubule identity Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processNotch signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Hairy/enhancer-of-split related with YRPW motif protein 1
Short name:
XHey-1
Alternative name(s):
Hairy and enhancer of split-related protein 1
Short name:
Hesr-1
Hairy-related transcription factor 1
Short name:
HRT-1
Short name:
XHRT1
Short name:
xHRT-1
Protein xbc8
Gene namesi
Name:hey1
Synonyms:bc8, hrt1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865637 hey1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002864261 – 294Hairy/enhancer-of-split related with YRPW motif protein 1Add BLAST294

Expressioni

Tissue specificityi

Expression in late gastrula and early neurula embryos is restricted to dorsal endodermal cells and the medial neural plate and in dorsal endodermal cells. Expression fills exactly the gap in between the two rows of motor neurons in the ventral neural tube. At later stages, expressed in the floor plate, in hypochord cells and in the somitogenic and anterior presomitic mesoderm. By tailbud stage, also highly expressed in the dorsal hindbrain, telencephalon and eye vesicles, olfactory placodes, branchial arches, tail fin and somites. Expression in the somite, eye, head and branchial arches persists throughout organogenesis. In addition, despite being absent in the early heart field, expression begins within the linear heart tube at stages 32-35. Also expressed from the early-tailbud stage in the dorsoanterior region of the developing pronephros. During early tailbud stages, localized to the most dorsoanterior portion of the pronephric anlagen. During late tailbud to early tadpole stages, expression marks the most dorsoanterior portion of the pronephros with expression becoming progressively restricted to the tip of the forming tubules. Pronephric expression is no longer detectable at late tadpole stages (stage 35). In adults, strongest expression is observed in the brain, eye, heart, lung, muscle, ovary, skin, spleen and testis.4 Publications

Developmental stagei

Expressed throughout embryogenesis, with expression rising after neurulation.1 Publication

Inductioni

By Notch signaling.3 Publications

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA in the form of homodimer or more strongly as a heterodimer with hes1/hairy1 or hes4-B/hairy2b. Also weakly interacts with the bHLH proteins hes2, neurod1 and neurod4/ath3. Interacts (via Orange domain) with ccdc89/boip (via C-terminus).2 Publications

GO - Molecular functioni

  • bHLH transcription factor binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi100520, 1 interactor
ELMiQ9I8A3

Structurei

3D structure databases

ProteinModelPortaliQ9I8A3
SMRiQ9I8A3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 99bHLHPROSITE-ProRule annotationAdd BLAST56
Domaini117 – 153OrangePROSITE-ProRule annotationAdd BLAST37

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi284 – 287YRPW motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi246 – 283Ser-richAdd BLAST38

Domaini

The Orange domain, downstream sequence and the bHLH are required for efficient heterodimerization with hes/hairy proteins, and for transcriptional repressor activity.
The C-terminal YRPW motif is not required for transcriptional repressor activity and is unable to recruit groucho.

Sequence similaritiesi

Belongs to the HEY family.Curated

Phylogenomic databases

HOVERGENiHBG003275
KOiK09091

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003650 Orange_dom
PfamiView protein in Pfam
PF07527 Hairy_orange, 1 hit
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00511 ORANGE, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS51054 ORANGE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9I8A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRGHDYSSS DSELDENIEV EKESADENGN LSSMSPSTSS QILARKRRRG
60 70 80 90 100
IIEKRRRDRI NNSLSELRRL VPSAFEKQGS AKLEKAEILQ MTVDHLKMLH
110 120 130 140 150
TAGGKGYFDA HALAMDYRSL GFRECLAEVA RYLSIIEGME TADPLRVRLV
160 170 180 190 200
SHLNNYASQR EAASTAHTSI GHIPWGGTFA HHPHLSHPLL LAQTAHTNST
210 220 230 240 250
SSSTEAHHHN RLRGSPHAES SSLRVAPNGN IASVLPVVAS SKLSPPLLSS
260 270 280 290
MASLSAFPFS FGSFHLLSPN SLSPTTPTPS GKPYRPWGTE IGAF
Length:294
Mass (Da):32,135
Last modified:October 1, 2000 - v1
Checksum:iB8A705A2F506C007
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401271 mRNA Translation: CAB96791.1
BC084410 mRNA Translation: AAH84410.1
RefSeqiNP_001083926.1, NM_001090457.1
UniGeneiXl.469

Genome annotation databases

GeneIDi399195
KEGGixla:399195

Similar proteinsi

Entry informationi

Entry nameiHEY1_XENLA
AccessioniPrimary (citable) accession number: Q9I8A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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