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Protein

Multifunctional protein ADE2

Gene

ade5

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.By similarity
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Multifunctional protein ADE2 (ade5)
  2. Adenylosuccinate lyase (AdSL), Adenylosuccinate lyase (AdSL)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Multifunctional protein ADE2 (ade5)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoribosylaminoimidazole carboxylase activity Source: FlyBase
  • phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: FlyBase

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: FlyBase

Keywordsi

Molecular functionDecarboxylase, Ligase, Lyase, Multifunctional enzyme
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.6 1994
ReactomeiR-DME-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00130
UPA00074; UER00131

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional protein ADE2
Alternative name(s):
Protein adenosine-5
Including the following 2 domains:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)
Alternative name(s):
AIR carboxylase
Short name:
AIRC
Gene namesi
Name:ade5Imported
ORF Names:CG3989
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0020513 ade5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000750351 – 429Multifunctional protein ADE2Add BLAST429

Proteomic databases

PaxDbiQ9I7S8
PRIDEiQ9I7S8

Expressioni

Gene expression databases

BgeeiFBgn0020513
ExpressionAtlasiQ9I7S8 baseline and differential
GenevisibleiQ9I7S8 DM

Interactioni

Protein-protein interaction databases

BioGridi58620, 5 interactors
IntActiQ9I7S8, 6 interactors
STRINGi7227.FBpp0073538

Structurei

3D structure databases

ProteinModelPortaliQ9I7S8
SMRiQ9I7S8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 264SAICAR synthetaseSequence analysisAdd BLAST258
Regioni265 – 429AIR carboxylaseSequence analysisAdd BLAST165

Sequence similaritiesi

In the N-terminal section; belongs to the SAICAR synthetase family.Sequence analysis
In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.Sequence analysis

Phylogenomic databases

eggNOGiKOG2835 Eukaryota
COG0041 LUCA
COG0152 LUCA
GeneTreeiENSGT00390000010172
InParanoidiQ9I7S8
KOiK01587
OrthoDBiEOG091G08DN
PhylomeDBiQ9I7S8

Family and domain databases

Gene3Di3.40.50.7700, 1 hit
HAMAPiMF_02045 PurE_classII, 1 hit
MF_00137 SAICAR_synth, 1 hit
InterProiView protein in InterPro
IPR033626 PurE_classII
IPR000031 PurE_dom
IPR035893 PurE_sf
IPR028923 SAICAR_synt/ADE2_N
IPR018236 SAICAR_synthetase_CS
PfamiView protein in Pfam
PF00731 AIRC, 1 hit
PF01259 SAICAR_synt, 1 hit
SMARTiView protein in SMART
SM01001 AIRC, 1 hit
SUPFAMiSSF52255 SSF52255, 1 hit
TIGRFAMsiTIGR01162 purE, 1 hit
PROSITEiView protein in PROSITE
PS01057 SAICAR_SYNTHETASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9I7S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTTTASIE GYKLGKVIIE GKTKQVYDLP EQPGLCLLLS KDRITAGDGV
60 70 80 90 100
KAHDLAGKAE ISNTTNGQVF RLLNEAGIRT AYVKQCGAKA FIARKCQMIP
110 120 130 140 150
IEWVTRRLAT GSFLKRNVGV PEGYRFSPPK QETFFKDDAN HDPQWSEEQI
160 170 180 190 200
VSAKFELNGL VIGQDEVDIM RRTTLLVFEI LERAWQTKNC ALIDMKVEFG
210 220 230 240 250
ICDDGNIVLA DIIDSDSWRL WPAGDKRLMV DKQVYRNLAS VTASDLDTVK
260 270 280 290 300
RNFIWVAEQL ADIVPKKDHL VVILMGSASD ISHSEKIATS CRSLGLNVEL
310 320 330 340 350
RVSSAHKGPE ETLRIVREYE SVMSNLIFVA VAGRSNGLGP VVSGSTNYPV
360 370 380 390 400
INCPPVKSDN MQVDVWSSLN LPSGLGCATV LYPEAAALHA ATILGLGNFM
410 420
VWSKLRVKAL NNFITLKKAD KELRGVRNA
Length:429
Mass (Da):47,281
Last modified:October 1, 2002 - v2
Checksum:iCF490A1D20CA2C7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102579 mRNA Translation: AAF06355.1
AF102580 Genomic DNA Translation: AAF06356.1
AE014298 Genomic DNA Translation: AAG22346.2
AY095520 mRNA Translation: AAM12252.1
RefSeqiNP_572826.1, NM_132598.4
UniGeneiDm.3315

Genome annotation databases

EnsemblMetazoaiFBtr0073705; FBpp0073538; FBgn0020513
GeneIDi32228
KEGGidme:Dmel_CG3989

Similar proteinsi

Entry informationi

Entry nameiPUR6_DROME
AccessioniPrimary (citable) accession number: Q9I7S8
Secondary accession number(s): Q9TVK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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