UniProtKB - Q9I7C4 (DPO3B_PSEAE)
Protein
Beta sliding clamp
Gene
dnaN
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity
GO - Molecular functioni
- 3'-5' exonuclease activity Source: InterPro
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- DNA strand elongation involved in DNA replication Source: PseudoCAP
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-2-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Beta sliding clampShort name: Beta clamp Short name: Sliding clamp Alternative name(s): Beta-clamp processivity factor DNA polymerase III beta sliding clamp subunit DNA polymerase III subunit beta |
Gene namesi | Name:dnaN Ordered Locus Names:PA0002 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA0002 |
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
- DNA polymerase III complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105453 | 1 – 367 | Beta sliding clampAdd BLAST | 367 |
Proteomic databases
PaxDbi | Q9I7C4 |
PRIDEi | Q9I7C4 |
Interactioni
Subunit structurei
Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA (PubMed:25170813). The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase (By similarity).
By similarity1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9I7C4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the beta sliding clamp family.Curated
Phylogenomic databases
HOGENOMi | CLU_038149_4_2_6 |
InParanoidi | Q9I7C4 |
OMAi | YLIMPVR |
PhylomeDBi | Q9I7C4 |
Family and domain databases
CDDi | cd00140, beta_clamp, 1 hit |
InterProi | View protein in InterPro IPR001001, DNA_polIII_beta IPR022635, DNA_polIII_beta_C IPR022637, DNA_polIII_beta_cen IPR022634, DNA_polIII_beta_N |
PANTHERi | PTHR30478, PTHR30478, 1 hit |
Pfami | View protein in Pfam PF00712, DNA_pol3_beta, 1 hit PF02767, DNA_pol3_beta_2, 1 hit PF02768, DNA_pol3_beta_3, 1 hit |
PIRSFi | PIRSF000804, DNA_pol_III_b, 1 hit |
SMARTi | View protein in SMART SM00480, POL3Bc, 1 hit |
TIGRFAMsi | TIGR00663, dnan, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9I7C4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHFTIQREAL LKPLQLVAGV VERRQTLPVL SNVLLVVEGQ QLSLTGTDLE
60 70 80 90 100
VELVGRVVLE DAAEPGEITV PARKLMDICK SLPNDVLIDI RVEEQKLLVK
110 120 130 140 150
AGRSRFTLST LPANDFPTVE EGPGSLNFSI AQSKLRRLID RTSFAMAQQD
160 170 180 190 200
VRYYLNGMLL EVNGGTLRSV ATDGHRLAMC SLDAQIPSQD RHQVIVPRKG
210 220 230 240 250
ILELARLLTE QDGEVGIVLG QHHIRATTGE FTFTSKLVDG KFPDYERVLP
260 270 280 290 300
RGGDKLVVGD RQQLREAFSR TAILSNEKYR GIRLQLSNGL LKIQANNPEQ
310 320 330 340 350
EEAEEEVQVE YNGGNLEIGF NVSYLLDVLG VIGTEQVRFI LSDSNSSALV
360
HEADNDDSAY VVMPMRL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG03392.1 |
PIRi | F83644 |
RefSeqi | NP_064722.1, NC_002516.2 WP_003097262.1, NZ_QZGE01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAG03392; AAG03392; PA0002 |
GeneIDi | 879244 |
KEGGi | pae:PA0002 |
PATRICi | fig|208964.12.peg.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG03392.1 |
PIRi | F83644 |
RefSeqi | NP_064722.1, NC_002516.2 WP_003097262.1, NZ_QZGE01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4TR8 | X-ray | 1.80 | A/B | 1-367 | [»] | |
4TSZ | X-ray | 2.00 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P | 1-367 | [»] | |
6AMS | X-ray | 2.39 | A/B/C/D | 1-367 | [»] | |
6PTH | X-ray | 3.05 | A | 1-367 | [»] | |
SMRi | Q9I7C4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PaxDbi | Q9I7C4 |
PRIDEi | Q9I7C4 |
Protocols and materials databases
DNASUi | 879244 |
Genome annotation databases
EnsemblBacteriai | AAG03392; AAG03392; PA0002 |
GeneIDi | 879244 |
KEGGi | pae:PA0002 |
PATRICi | fig|208964.12.peg.2 |
Organism-specific databases
PseudoCAPi | PA0002 |
Phylogenomic databases
HOGENOMi | CLU_038149_4_2_6 |
InParanoidi | Q9I7C4 |
OMAi | YLIMPVR |
PhylomeDBi | Q9I7C4 |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-2-MONOMER |
Family and domain databases
CDDi | cd00140, beta_clamp, 1 hit |
InterProi | View protein in InterPro IPR001001, DNA_polIII_beta IPR022635, DNA_polIII_beta_C IPR022637, DNA_polIII_beta_cen IPR022634, DNA_polIII_beta_N |
PANTHERi | PTHR30478, PTHR30478, 1 hit |
Pfami | View protein in Pfam PF00712, DNA_pol3_beta, 1 hit PF02767, DNA_pol3_beta_2, 1 hit PF02768, DNA_pol3_beta_3, 1 hit |
PIRSFi | PIRSF000804, DNA_pol_III_b, 1 hit |
SMARTi | View protein in SMART SM00480, POL3Bc, 1 hit |
TIGRFAMsi | TIGR00663, dnan, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO3B_PSEAE | |
Accessioni | Q9I7C4Primary (citable) accession number: Q9I7C4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 4, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families