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UniProtKB - Q9I6R0 (PCAF_PSEAE)
Protein
Beta-ketoadipyl-CoA thiolase
Gene
pcaF
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA.
By similarityCatalytic activityi
- EC:2.3.1.174
: beta-ketoadipate pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate. This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA and succinyl-CoA from 3-oxoadipate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | Acyl-thioester intermediateBy similarity | 1 | |
Active sitei | 357 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 387 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 3-oxoadipyl-CoA thiolase activity Source: UniProtKB-EC
- acetyl-CoA C-acyltransferase activity Source: GO_Central
GO - Biological processi
- 3,4-dihydroxybenzoate catabolic process Source: InterPro
- beta-ketoadipate pathway Source: UniProtKB-UniPathway
- fatty acid beta-oxidation Source: GO_Central
- phenylacetate catabolic process Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Aromatic hydrocarbons catabolism |
Enzyme and pathway databases
UniPathwayi | UPA00157;UER00263 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-ketoadipyl-CoA thiolase (EC:2.3.1.174)Alternative name(s): 3-oxoadipyl-CoA thiolase |
Gene namesi | Name:pcaF Ordered Locus Names:PA0228 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA0228 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000287819 | 1 – 401 | Beta-ketoadipyl-CoA thiolaseAdd BLAST | 401 |
Proteomic databases
PaxDbi | Q9I6R0 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_031026_2_2_6 |
InParanoidi | Q9I6R0 |
OMAi | NASPMND |
PhylomeDBi | Q9I6R0 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR012793, PcaF IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02430, pcaF, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9I6R0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSREVFICDA VRTPIGRFGG SLSAVRADDL AAVPLKALVE RNPGVDWSAL
60 70 80 90 100
DEVFLGCANQ AGEDNRNVAR MALLLAGLPE SVPGVTLNRL CASGMDAIGT
110 120 130 140 150
AFRAIACGEM ELAIAGGVES MSRAPYVMGK ADSAFGRGQK IEDTTIGWRF
160 170 180 190 200
VNPLMKEQYG IDPMPQTADN VADDYRVSRA DQDAFALRSQ QRAGRAQEAG
210 220 230 240 250
FFAEEIVPVT IRGRKGDTLV EHDEHPRPDT TLEALARLKP VNGPEKTVTA
260 270 280 290 300
GNASGVNDGA AALVLASAEA VEKHGLTPRA RVLGMASAGV APRIMGIGPV
310 320 330 340 350
PAVRKLLRRL DLAIDAFDVI ELNEAFASQG LACLRELGVA DDSEKVNPNG
360 370 380 390 400
GAIALGHPLG MSGARLVLTA LHQLEKSGGR RGLATMCVGV GQGLALAIER
V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG03617.1 |
PIRi | D83618 |
RefSeqi | NP_248919.1, NC_002516.2 WP_003101891.1, NZ_QZGE01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAG03617; AAG03617; PA0228 |
GeneIDi | 877715 |
KEGGi | pae:PA0228 |
PATRICi | fig|208964.12.peg.238 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG03617.1 |
PIRi | D83618 |
RefSeqi | NP_248919.1, NC_002516.2 WP_003101891.1, NZ_QZGE01000024.1 |
3D structure databases
AlphaFoldDBi | Q9I6R0 |
SMRi | Q9I6R0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 287.DR97_3185 |
Proteomic databases
PaxDbi | Q9I6R0 |
Genome annotation databases
EnsemblBacteriai | AAG03617; AAG03617; PA0228 |
GeneIDi | 877715 |
KEGGi | pae:PA0228 |
PATRICi | fig|208964.12.peg.238 |
Organism-specific databases
PseudoCAPi | PA0228 |
Phylogenomic databases
HOGENOMi | CLU_031026_2_2_6 |
InParanoidi | Q9I6R0 |
OMAi | NASPMND |
PhylomeDBi | Q9I6R0 |
Enzyme and pathway databases
UniPathwayi | UPA00157;UER00263 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR012793, PcaF IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit TIGR02430, pcaF, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PCAF_PSEAE | |
Accessioni | Q9I6R0Primary (citable) accession number: Q9I6R0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families