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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei412NucleophileUniRule annotation1
Active sitei465Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-2193-MONOMER
UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:PA2153
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2153

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887301 – 7321,4-alpha-glucan branching enzyme GlgBAdd BLAST732

Proteomic databases

PaxDbiQ9I1W2

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA2153

Structurei

3D structure databases

ProteinModelPortaliQ9I1W2
SMRiQ9I1W2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C Bacteria
COG0296 LUCA
HOGENOMiHOG000283037
InParanoidiQ9I1W2
KOiK00700
OMAiEVVHGKS
PhylomeDBiQ9I1W2

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

Q9I1W2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPMSERER WQLELDKLQR GLQGDPFAFL GPQRDPGGEG GVLRAYLPGA
60 70 80 90 100
QRVELLDEDG ATLAELEQSD PGSGLFQRHL ERLPPRYRLR VHWPDGVQES
110 120 130 140 150
EDPYAFGPLL GELDLYLFAE GNHRQLASCL GAQLTRHEGV EGVRFAVWAP
160 170 180 190 200
NAVRVSVVGD FNGWDGRRHP MRRRYPSGVW ELFVPRLGEG ELYKYELQGH
210 220 230 240 250
DGLLPLKADP LALACETPPG TASKTCAALR HEWRDQDWLA RRAERQGYAA
260 270 280 290 300
PLSIYEVHAG SWRHRDGRPP HWSELAEELI PYVRQLGFTH IELMPVMEHP
310 320 330 340 350
FGGSWGYQPL GLFAPTARYG TPEDFAAFVD ACHQAGIGVI LDWVPAHFPT
360 370 380 390 400
DAHGLGRFDG TALYEYEHPF EGFHQDWDTY IYNLGRSEVH GFMLASALHW
410 420 430 440 450
LRTYHVDGLR VDAVASMLYR DYSRKEGEWL PNRHGGRENL EAIDFLHHLN
460 470 480 490 500
QVVASETPGA LVIAEESTAW PGVSRPVAEG GLGFSHKWNM GWMHDSLAYI
510 520 530 540 550
GEDPLHRRYH HHQLTFGLLY AFSEHFILPI SHDEVVHGKH SLLDKMPGDR
560 570 580 590 600
WQKFANLRLY LSFMWSHPGK KLLFMGCEFG QWREWNHDGE LDWYLLRYPE
610 620 630 640 650
HQGVQDLVAA LNRLYRELPA LHARDGEALG FEWLIGDDQA NSVYAWLRHA
660 670 680 690 700
AGEPSLLAVH NFTPVPRQGY RIGVPQGGDW DVLLNSDAQA FAGSGAGSQG
710 720 730
RVSSESCGAH GQAQSLVLDL PPLGTLLLRP AG
Length:732
Mass (Da):82,563
Last modified:March 1, 2001 - v1
Checksum:iC31303D6D5F929F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG05541.1
PIRiH83376
RefSeqiNP_250843.1, NC_002516.2
WP_003113646.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG05541; AAG05541; PA2153
GeneIDi881350
KEGGipae:PA2153
PATRICifig|208964.12.peg.2252

Similar proteinsi

Entry informationi

Entry nameiGLGB_PSEAE
AccessioniPrimary (citable) accession number: Q9I1W2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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