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Entry version 111 (12 Aug 2020)
Sequence version 1 (01 Mar 2001)
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Protein

Hydrogen cyanide synthase subunit HcnB

Gene

hcnB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

A three-component membrane-bound flavoenzyme that catalyzes the formation of hydrogen cyanide, a secondary metabolite, by transfer of electrons to a cyanide-resistant branch of the aerobic respiratory chain.4 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Oxygen is necessary for cyanogenesis. Activated by succinate, glycine methyl ester, glucose and D,L-methionine in addition to glycine. Phenazine methosulfate, methylene blue, 2,6-dichlorophenolindophenol (DCIP) and ferricyanide can replace oxygen for the reaction. Inhibited by pyrrolnitrin and acriflavine at 1 mM concentration.2 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Measured for the whole complex.1 Publication
  1. KM=50 mM for glycine3 Publications

    pH dependencei

    Optimum pH is 8.3 (in the presence of Tris-HCl). Active from 7.3-7.8 in the presence of other buffers.3 Publications

    Temperature dependencei

    Not stable at 0 degrees Celsius. Decrease in activity up to 40% immediately after freeze-drying procedure, with a further decrease up to 10% when stored at -10 degrees Celsius.3 Publications

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    PAER208964:G1FZ6-2234-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Hydrogen cyanide synthase subunit HcnBImported (EC:1.4.99.52 Publications)
    Short name:
    HcnB1 Publication
    Alternative name(s):
    Glycine dehydrogenase (cyanide-forming)1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:hcnBImported
    Ordered Locus Names:PA2194
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri208964 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000002438 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    Organism-specific databases

    Pseudomonas genome database

    More...
    PseudoCAPi
    PA2194

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    • Cell membrane 1 Publication

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell membrane, Membrane

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004198111 – 464Hydrogen cyanide synthase subunit HcnBAdd BLAST464

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    Q9I1S2

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q9I1S2

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Reduced oxygen levels.1 Publication

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Heterotrimer of HcnA, HcnB and HcnC.

    Curated

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Phylogenomic databases

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_030705_1_0_6

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q9I1S2

    KEGG Orthology (KO)

    More...
    KOi
    K10815

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    GACQGRF

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    Q9I1S2

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.10.1100, 1 hit
    3.50.50.60, 2 hits

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR007419, BFD-like_2Fe2S-bd_dom
    IPR041854, BFD-like_2Fe2S-bd_dom_sf
    IPR036188, FAD/NAD-bd_sf
    IPR023753, FAD/NAD-binding_dom
    IPR017224, Opine_Oxase_asu/HCN_bsu

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF04324, Fer2_BFD, 1 hit
    PF07992, Pyr_redox_2, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF037495, Opine_OX_OoxA/HcnB, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51905, SSF51905, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q9I1S2-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MNLRPVIVGG GSAGMAAAIE LARRGVPCVL FDEASRPGGV VYRGPLRAGV
    60 70 80 90 100
    DPAYLGARYT RMLEKLRRDF SACAGHIDLR LNSRVVGGDG QRLMVLDEAE
    110 120 130 140 150
    RLHEVEYSHL LLATGCHERS VPFPGWTLPG VMLLGGLQLQ IKSGVVKPLG
    160 170 180 190 200
    DTLIAGSGPL LPLVACQLHA AGVRVAGVYE ACAFGRMARE SLALLNKPQL
    210 220 230 240 250
    FLDGLSMLGY LKLNGIPLHY GWGVVEASGD GELTEVTVAP YDEEWRPDLE
    260 270 280 290 300
    NARPVKASTL AVGYGFIPRT QLSQQLGLEH GFSDDGYLRA ECNVWQQSSQ
    310 320 330 340 350
    PHIHLAGDMA GIRGGEAAMI GGRIAALSIL LQREAIAPAE AIERRESHLA
    360 370 380 390 400
    RLEAIKRFRA GVERYTQRGA RQVELARADT VICRCEQVTR GDIERALEQG
    410 420 430 440 450
    VQDIAGLKMR TRAGMGDCQG RMCIGYCSDR LRRATGRHDV GWLRPRFPID
    460
    PIPFSAFQNL GTEA
    Length:464
    Mass (Da):50,389
    Last modified:March 1, 2001 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3E77853869B61ADD
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti371R → A in AAF21029 (PubMed:11092854).Curated1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AF208523 Genomic DNA Translation: AAF21029.2
    AE004091 Genomic DNA Translation: AAG05582.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    G83370

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_250884.1, NC_002516.2
    WP_003113685.1, NZ_QZGE01000014.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAG05582; AAG05582; PA2194

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    882182

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    pae:PA2194

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|208964.12.peg.2298

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF208523 Genomic DNA Translation: AAF21029.2
    AE004091 Genomic DNA Translation: AAG05582.1
    PIRiG83370
    RefSeqiNP_250884.1, NC_002516.2
    WP_003113685.1, NZ_QZGE01000014.1

    3D structure databases

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    SWISS-MODEL Interactive Workspace

    More...
    SWISS-MODEL-Workspacei
    Submit a new modelling project...

    Proteomic databases

    PaxDbiQ9I1S2
    PRIDEiQ9I1S2

    Genome annotation databases

    EnsemblBacteriaiAAG05582; AAG05582; PA2194
    GeneIDi882182
    KEGGipae:PA2194
    PATRICifig|208964.12.peg.2298

    Organism-specific databases

    PseudoCAPiPA2194

    Phylogenomic databases

    HOGENOMiCLU_030705_1_0_6
    InParanoidiQ9I1S2
    KOiK10815
    OMAiGACQGRF
    PhylomeDBiQ9I1S2

    Enzyme and pathway databases

    BioCyciPAER208964:G1FZ6-2234-MONOMER

    Family and domain databases

    Gene3Di1.10.10.1100, 1 hit
    3.50.50.60, 2 hits
    InterProiView protein in InterPro
    IPR007419, BFD-like_2Fe2S-bd_dom
    IPR041854, BFD-like_2Fe2S-bd_dom_sf
    IPR036188, FAD/NAD-bd_sf
    IPR023753, FAD/NAD-binding_dom
    IPR017224, Opine_Oxase_asu/HCN_bsu
    PfamiView protein in Pfam
    PF04324, Fer2_BFD, 1 hit
    PF07992, Pyr_redox_2, 1 hit
    PIRSFiPIRSF037495, Opine_OX_OoxA/HcnB, 1 hit
    SUPFAMiSSF51905, SSF51905, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHCNB_PSEAE
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9I1S2
    Secondary accession number(s): Q9REV8
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: March 1, 2001
    Last modified: August 12, 2020
    This is version 111 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome
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