UniProtKB - Q9I0J6 (NUOG_PSEAE)
Protein
NADH-quinone oxidoreductase subunit G
Gene
nuoG
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 34 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 45 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 48 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 67 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 99 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 103 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 106 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 112 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 151 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 154 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 157 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 201 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 228 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 231 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 235 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 263 | Iron-sulfur 4 (4Fe-4S)Sequence analysis | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
- cellular respiration Source: GO_Central
Keywordsi
Molecular function | Translocase |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-2682-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit G (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit G NDH-1 subunit G |
Gene namesi | Name:nuoG Ordered Locus Names:PA2642 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA2642 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118549 | 1 – 905 | NADH-quinone oxidoreductase subunit GAdd BLAST | 905 |
Keywords - PTMi
QuinoneProteomic databases
PaxDbi | Q9I0J6 |
PRIDEi | Q9I0J6 |
Interactioni
Subunit structurei
Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 83 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 83 – 122 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 221 – 277 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Phylogenomic databases
HOGENOMi | CLU_000422_11_4_6 |
InParanoidi | Q9I0J6 |
OMAi | PSICHGC |
PhylomeDBi | Q9I0J6 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR009010, Asp_de-COase-like_dom_sf IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9I0J6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATIHVDGKT LEVDGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV
60 70 80 90 100
KQYTDENDKR GRLVMSCMTP ATDNTWISIE DEEAKQFRAS VVEWLMTNHP
110 120 130 140 150
HDCPVCEEGG HCHLQDMTVM TGHNERRYRF TKRTHQNQEL GPFIAHEMNR
160 170 180 190 200
CIACYRCVRY YKDYAGGTDL GVYGAHDNVY FGRVEDGVLE SEFSGNLTEV
210 220 230 240 250
CPTGVFTDKT HSERYNRKWD MQFAPSICHG CSSGCNISPG ERYGEIRRIE
260 270 280 290 300
NRYNGSVNHY FLCDRGRFGY GYVNREDRPR QPLLVLSKQK LSLDGALDQA
310 320 330 340 350
AALLKERKVV GIGSPRASLE SNFALRELVG EGNFYSGINE GELDRLRLIL
360 370 380 390 400
QVMQEGPLPV PSIRDIEDHD AVFVLGEDLT QTAARIALAL RQSVKGKAVE
410 420 430 440 450
MAADMKVQPW LDAAVKNIAQ HAQNPLFIAS VSATRLDDVA EETVHAAPDD
460 470 480 490 500
LARLGFAVAH AIDPSAPSVA DLDPQAQAFA QRITDALLAA KRPLVVSGNS
510 520 530 540 550
LGNKALIEAA ANIAKALKQR EKNGSISLVV GEANSLGLAL FGGDSVEAAL
560 570 580 590 600
ERLTSGQADA VVVLENDLYR RTDAARVDAA LAAAKVVIVA DHQQTATTAK
610 620 630 640 650
AHLVLPAASF AEGDGTLVSQ EGRAQRFFQV FDPTYYDARN MVREGWRWLH
660 670 680 690 700
AIHSTLQGKR VDWTQLDHVT EAVAEAKPIL AGIRDAAPAA SFRIKGLKLA
710 720 730 740 750
REPHRYSGRT AMRANISVHE PRTPQDIDSA FAFSMEGYSG SQEDRQQIPF
760 770 780 790 800
AWSPGWNSPQ AWNKFQDEVG GHLRAGDPGV RLIEPKGEGL DWFQAVPVPF
810 820 830 840 850
SAKADSWKVV PLYHLFGSEE NSSRAAPIQQ RIPETYVALS KEDADRLGVN
860 870 880 890 900
DGATLGFQLK GQALRLPLRI DEQLGAGLIG LPVGFAGIPA AIAGCSVEGL
QEAAQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG06030.1 |
PIRi | G83314 |
RefSeqi | NP_251332.1, NC_002516.2 WP_003113377.1, NZ_QZGE01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAG06030; AAG06030; PA2642 |
GeneIDi | 882349 |
KEGGi | pae:PA2642 |
PATRICi | fig|208964.12.peg.2765 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG06030.1 |
PIRi | G83314 |
RefSeqi | NP_251332.1, NC_002516.2 WP_003113377.1, NZ_QZGE01000008.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PaxDbi | Q9I0J6 |
PRIDEi | Q9I0J6 |
Genome annotation databases
EnsemblBacteriai | AAG06030; AAG06030; PA2642 |
GeneIDi | 882349 |
KEGGi | pae:PA2642 |
PATRICi | fig|208964.12.peg.2765 |
Organism-specific databases
PseudoCAPi | PA2642 |
Phylogenomic databases
HOGENOMi | CLU_000422_11_4_6 |
InParanoidi | Q9I0J6 |
OMAi | PSICHGC |
PhylomeDBi | Q9I0J6 |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-2682-MONOMER |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR009010, Asp_de-COase-like_dom_sf IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOG_PSEAE | |
Accessioni | Q9I0J6Primary (citable) accession number: Q9I0J6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | March 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families