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Protein

D-alanine--D-alanine ligase A

Gene

ddlA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi286Magnesium or manganese 1UniRule annotation1
Metal bindingi299Magnesium or manganese 1UniRule annotation1
Metal bindingi299Magnesium or manganese 2UniRule annotation1
Metal bindingi301Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 217ATPUniRule annotationAdd BLAST54

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-4274-MONOMER
UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligase AUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligase AUniRule annotation
D-alanylalanine synthetase AUniRule annotation
Gene namesi
Name:ddlAUniRule annotation
Ordered Locus Names:PA4201
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4201

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001778551 – 346D-alanine--D-alanine ligase AAdd BLAST346

Proteomic databases

PaxDbiQ9HWI0

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4201

Structurei

3D structure databases

ProteinModelPortaliQ9HWI0
SMRiQ9HWI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 332ATP-graspUniRule annotationAdd BLAST195

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011592
InParanoidiQ9HWI0
KOiK01921
OMAiYETKYTE
PhylomeDBiQ9HWI0

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HWI0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGQDKLKVAV LFGGSSEERD VSIASGAQVI QALRSAGHQV LAVDTASGLL
60 70 80 90 100
GAEEERRLLA SKVKEVPPDS DSLAIIRSGK QSLLSAGELA GVDVFFLALH
110 120 130 140 150
GGTGEDGTLQ ALLDAGGFAY TGSGHLASAM AMDKDVAKRL FLAAGVETAS
160 170 180 190 200
WLMAPASEEE VREQLGFPLV VKPNSQGSTV GLSIVHSQAE LQPAIELAGR
210 220 230 240 250
YGDEVMLERF VAGREVTVGV LDDQALPVGE ILLGGQEVFD YEHKYQAGAV
260 270 280 290 300
REVFPADLPP AIAAEAQRLA LKVHRALKLS GYSRTDFRLD EQGRLWCLEV
310 320 330 340
NTLPGMTATS LLPQAAAAAG IGFAELCERI CRLGIERCKG ARKARS
Length:346
Mass (Da):36,534
Last modified:March 1, 2001 - v1
Checksum:iA66C5B0DB58F9A23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG07588.1
PIRiE83121
RefSeqiNP_252890.1, NC_002516.2
WP_003114722.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG07588; AAG07588; PA4201
GeneIDi879033
KEGGipae:PA4201
PATRICifig|208964.12.peg.4401

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG07588.1
PIRiE83121
RefSeqiNP_252890.1, NC_002516.2
WP_003114722.1, NC_002516.2

3D structure databases

ProteinModelPortaliQ9HWI0
SMRiQ9HWI0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4201

Proteomic databases

PaxDbiQ9HWI0

Protocols and materials databases

DNASUi879033
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07588; AAG07588; PA4201
GeneIDi879033
KEGGipae:PA4201
PATRICifig|208964.12.peg.4401

Organism-specific databases

PseudoCAPiPA4201

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011592
InParanoidiQ9HWI0
KOiK01921
OMAiYETKYTE
PhylomeDBiQ9HWI0

Enzyme and pathway databases

UniPathwayi
UPA00219

BioCyciPAER208964:G1FZ6-4274-MONOMER

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDLA_PSEAE
AccessioniPrimary (citable) accession number: Q9HWI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2001
Last modified: October 10, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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