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Protein

5-methylphenazine-1-carboxylate 1-monooxygenase

Gene

phzS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of pyocyanine, a blue-pigmented phenazine derivative, which plays a role in virulence. Catalyzes the oxidative decarboxylation of 5-methylphenazine-1-carboxylate (5-methyl-PCA) to pyocyanine. Can also act on phenazine-1-carboxylate (PCA), converting it into 1-hydroxyphenazine (1-HP). However, PCA is a poor substrate.1 Publication

Catalytic activityi

5-methylphenazine-1-carboxylate + NADH + O2 = pyocyanine + NAD+ + CO2 + H2O.1 Publication

Cofactori

FAD2 Publications

Pathwayi: pyocyanine biosynthesis

This protein is involved in the pathway pyocyanine biosynthesis, which is part of Secondary metabolite biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway pyocyanine biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106FADCombined sources2 Publications1
Binding sitei132FAD; via amide nitrogen and carbonyl oxygenCombined sources2 Publications1
Binding sitei191FADCombined sources2 Publications1
Binding sitei310FADCombined sources2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 15FADCombined sources2 Publications2
Nucleotide bindingi35 – 36FADCombined sources2 Publications2
Nucleotide bindingi43 – 45FADCombined sources2 Publications3

GO - Molecular functioni

  • FAD binding Source: InterPro
  • monooxygenase activity Source: PseudoCAP

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16022
PAER208964:G1FZ6-4290-MONOMER
UniPathwayiUPA00235

Names & Taxonomyi

Protein namesi
Recommended name:
5-methylphenazine-1-carboxylate 1-monooxygenaseCurated (EC:1.14.13.2181 Publication)
Gene namesi
Name:phzS1 Publication
Ordered Locus Names:PA4217Imported
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4217

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004417081 – 4025-methylphenazine-1-carboxylate 1-monooxygenaseAdd BLAST402

Proteomic databases

PaxDbiQ9HWG9

Interactioni

Subunit structurei

Monomer in solution. Probably interacts transiently with PhzM.1 Publication

Protein-protein interaction databases

STRINGi208964.PA4217

Structurei

Secondary structure

1402
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi14 – 25Combined sources12
Beta strandi29 – 39Combined sources11
Beta strandi46 – 49Combined sources4
Helixi51 – 59Combined sources9
Helixi63 – 69Combined sources7
Beta strandi70 – 73Combined sources4
Beta strandi75 – 79Combined sources5
Beta strandi85 – 90Combined sources6
Helixi92 – 95Combined sources4
Beta strandi101 – 105Combined sources5
Helixi106 – 121Combined sources16
Beta strandi125 – 138Combined sources14
Beta strandi141 – 148Combined sources8
Beta strandi153 – 163Combined sources11
Helixi170 – 175Combined sources6
Beta strandi183 – 197Combined sources15
Beta strandi202 – 209Combined sources8
Beta strandi215 – 220Combined sources6
Helixi223 – 226Combined sources4
Turni227 – 229Combined sources3
Beta strandi231 – 240Combined sources10
Helixi241 – 244Combined sources4
Helixi259 – 266Combined sources8
Beta strandi272 – 274Combined sources3
Helixi276 – 281Combined sources6
Beta strandi284 – 293Combined sources10
Beta strandi305 – 307Combined sources3
Helixi310 – 313Combined sources4
Helixi323 – 339Combined sources17
Helixi343 – 377Combined sources35

3D structure databases

ProteinModelPortaliQ9HWG9
SMRiQ9HWG9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HWG9

Family & Domainsi

Phylogenomic databases

eggNOGiENOG41061H0 Bacteria
COG0654 LUCA
HOGENOMiHOG000166536
InParanoidiQ9HWG9
KOiK20940
OMAiMYPTGSN
PhylomeDBiQ9HWG9

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR002938 FAD-bd
IPR036188 FAD/NAD-bd_sf
PfamiView protein in Pfam
PF01494 FAD_binding_3, 2 hits
SUPFAMiSSF51905 SSF51905, 2 hits

Sequencei

Sequence statusi: Complete.

Q9HWG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPIDILIA GAGIGGLSCA LALHQAGIGK VTLLESSSEI RPLGVGINIQ
60 70 80 90 100
PAAVEALAEL GLGPALAATA IPTHELRYID QSGATVWSEP RGVEAGNAYP
110 120 130 140 150
QYSIHRGELQ MILLAAVRER LGQQAVRTGL GVERIEERDG RVLIGARDGH
160 170 180 190 200
GKPQALGADV LVGADGIHSA VRAHLHPDQR PLSHGGITMW RGVTEFDRFL
210 220 230 240 250
DGKTMIVAND EHWSRLVAYP ISARHAAEGK SLVNWVCMVP SAAVGQLDNE
260 270 280 290 300
ADWNRDGRLE DVLPFFADWD LGWFDIRDLL TRNQLILQYP MVDRDPLPHW
310 320 330 340 350
GRGRITLLGD AAHLMYPMGA NGASQAILDG IELAAALARN ADVAAALREY
360 370 380 390 400
EEARRPTANK IILANREREK EEWAAASRPK TEKSAALEAI TGSYRNQVER

PR
Length:402
Mass (Da):43,645
Last modified:March 1, 2001 - v1
Checksum:iF2BB36662ABAC790
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG07605.1
PIRiE83119
RefSeqiNP_252907.1, NC_002516.2
WP_003115086.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG07605; AAG07605; PA4217
GeneIDi881836
KEGGipae:PA4217
PATRICifig|208964.12.peg.4418

Similar proteinsi

Entry informationi

Entry nameiPHZS_PSEAE
AccessioniPrimary (citable) accession number: Q9HWG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2017
Last sequence update: March 1, 2001
Last modified: February 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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