UniProtKB - Q9HVZ7 (Q9HVZ7_PSEAE)
Protein
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Gene
murF
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation
Catalytic activityi
- ATP + D-alanyl-D-alanine + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanineUniRule annotationEC:6.3.2.10UniRule annotation
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 54 | UDPCombined sources | 1 | |
Metal bindingi | 114 | MagnesiumCombined sources | 1 | |
Metal bindingi | 140 | MagnesiumCombined sources | 1 | |
Binding sitei | 140 | ATP analogCombined sources | 1 | |
Metal bindingi | 164 | MagnesiumCombined sources | 1 | |
Binding sitei | 189 | ATP analogCombined sources | 1 | |
Binding sitei | 288 | ATP analogCombined sources | 1 | |
Binding sitei | 292 | ATP analogCombined sources | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 33 | UDPCombined sources | 4 | |
Nucleotide bindingi | 47 – 49 | UDPCombined sources | 3 | |
Nucleotide bindingi | 109 – 115 | ATPUniRule annotation | 7 | |
Nucleotide bindingi | 111 – 115 | ATP analogCombined sources | 5 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | LigaseUniRule annotationImportedARBA annotation |
Biological process | Cell cycle, Cell divisionUniRule annotationARBA annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotationARBA annotation, Peptidoglycan synthesisUniRule annotationARBA annotation |
Ligand | ATP-bindingUniRule annotation, MagnesiumCombined sources, Metal-bindingCombined sources, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-4503-MONOMER |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)Alternative name(s): D-alanyl-D-alanine-adding enzymeUniRule annotation |
Gene namesi | Name:murFUniRule annotationImported Ordered Locus Names:PA4416Imported |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)Imported |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA4416 |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 93 | Mur_ligaseInterPro annotationAdd BLAST | 68 | |
Domaini | 108 – 300 | Mur_ligase_MInterPro annotationAdd BLAST | 193 | |
Domaini | 320 – 391 | Mur_ligase_CInterPro annotationAdd BLAST | 72 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_031507_1_1_6 |
InParanoidi | Q9HVZ7 |
OMAi | SYNNHWG |
PhylomeDBi | Q9HVZ7 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9HVZ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEPLRLSQL TVALDARLIG EDAVFSAVST DSRAIGPGQL FIALSGPRFD
60 70 80 90 100
GHDYLAEVAA KGAVAALVER EVADAPLPQL LVRDTRAALG RLGALNRRKF
110 120 130 140 150
TGPLAAMTGS SGKTTVKEML ASILRTQAGD AESVLATRGN LNNDLGVPLT
160 170 180 190 200
LLQLAPQHRS AVIELGASRI GEIAYTVELT RPHVAIITNA GTAHVGEFGG
210 220 230 240 250
PEKIVEAKGE ILEGLAADGT AVLNLDDKAF DTWKARASGR PLLTFSLDRP
260 270 280 290 300
QADFRAADLQ RDARGCMGFR LQGVAGEAQV QLNLLGRHNV ANALAAAAAA
310 320 330 340 350
HALGVPLDGI VAGLQALQPV KGRAVAQLTA SGLRVIDDSY NANPASMLAA
360 370 380 390 400
IDILSGFSGR TVLVLGDMGE LGSWAEQAHR EVGAYAAGKV SALYAVGPLM
410 420 430 440 450
AHAVQAFGAT GRHFADQASL IGALATEQPT TTILIKGSRS AAMDKVVAAL
CGSSEESH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG07804.1 |
PIRi | A83095 |
RefSeqi | NP_253106.1, NC_002516.2 WP_003103103.1, NZ_QZGE01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAG07804; AAG07804; PA4416 |
GeneIDi | 881289 |
KEGGi | pae:PA4416 |
PATRICi | fig|208964.12.peg.4625 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG07804.1 |
PIRi | A83095 |
RefSeqi | NP_253106.1, NC_002516.2 WP_003103103.1, NZ_QZGE01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4CVK | X-ray | 1.92 | A | 1-458 | [»] | |
4CVL | X-ray | 2.98 | A | 1-458 | [»] | |
4CVM | X-ray | 2.06 | A | 1-458 | [»] | |
SMRi | Q9HVZ7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PaxDbi | Q9HVZ7 |
PRIDEi | Q9HVZ7 |
Genome annotation databases
EnsemblBacteriai | AAG07804; AAG07804; PA4416 |
GeneIDi | 881289 |
KEGGi | pae:PA4416 |
PATRICi | fig|208964.12.peg.4625 |
Organism-specific databases
PseudoCAPi | PA4416 |
Phylogenomic databases
HOGENOMi | CLU_031507_1_1_6 |
InParanoidi | Q9HVZ7 |
OMAi | SYNNHWG |
PhylomeDBi | Q9HVZ7 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | PAER208964:G1FZ6-4503-MONOMER |
Miscellaneous databases
PHI-basei | PHI:7632 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q9HVZ7_PSEAE | |
Accessioni | Q9HVZ7Primary (citable) accession number: Q9HVZ7 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | March 1, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |