UniProtKB - Q9HVM7 (ISPH_PSEAE)
Protein
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Gene
ispH
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation
Catalytic activityi
- H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin]UniRule annotationEC:1.17.7.4UniRule annotation
- dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin]UniRule annotationEC:1.17.7.4UniRule annotation
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation
: dimethylallyl diphosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.
Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway
This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotationProteins known to be involved in the 6 steps of the subpathway in this organism are:
- 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
- 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
- 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
- 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
- 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
- 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 12 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Binding sitei | 41 | SubstrateUniRule annotation | 1 | |
Binding sitei | 74 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 96 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Binding sitei | 124 | SubstrateUniRule annotation | 1 | |
Active sitei | 126 | Proton donorUniRule annotation | 1 | |
Binding sitei | 168 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 198 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Binding sitei | 270 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Source: UniProtKB-UniRule
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- dimethylallyl diphosphate biosynthetic process Source: UniProtKB-UniRule
- isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniRule
- terpenoid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Isoprene biosynthesis |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-4650-MONOMER |
UniPathwayi | UPA00056;UER00097 UPA00059;UER00105 |
Names & Taxonomyi
Protein namesi | Recommended name: 4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)Short name: HMBPP reductaseUniRule annotation |
Gene namesi | Name:ispHUniRule annotation Synonyms:lytB Ordered Locus Names:PA4557 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA4557 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000128857 | 1 – 314 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST | 314 |
Proteomic databases
PaxDbi | Q9HVM7 |
PRIDEi | Q9HVM7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 226 – 228 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the IspH family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_027486_1_0_6 |
InParanoidi | Q9HVM7 |
OMAi | HNKYVVD |
PhylomeDBi | Q9HVM7 |
Family and domain databases
CDDi | cd13944, lytB_ispH, 1 hit |
HAMAPi | MF_00191, IspH, 1 hit |
InterProi | View protein in InterPro IPR003451, LytB/IspH |
PANTHERi | PTHR30426, PTHR30426, 1 hit |
Pfami | View protein in Pfam PF02401, LYTB, 1 hit |
TIGRFAMsi | TIGR00216, ispH_lytB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9HVM7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQIKLANPRG FCAGVDRAIE IVNRALDVFG PPIYVRHEVV HNKFVVDNLR
60 70 80 90 100
QRGAIFVEEL DQVPNNVIVI FSAHGVSQAV RKEAEGRGLK VFDATCPLVT
110 120 130 140 150
KVHMEVVRYS RDGHECVLIG HEGHPEVEGT MGQYDASNGG AIYLVEDEAD
160 170 180 190 200
VAALEVRKPE ALHYVTQTTL SMDDTSKVID ALRAKFPQIQ GPRKNDICYA
210 220 230 240 250
TQNRQDAVKE LADQCDMVLV VGSPNSSNSN RLRELAERMG TPAYLIDGAE
260 270 280 290 300
DMQRGWFDGV RRIGITAGAS APEVLVRGVI AQLREWGASE EQELEGREEN
310
ITFSMPKELR VKAL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 65 | N → D in AAP81279 (PubMed:14983043).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY273871 Genomic DNA Translation: AAP81279.1 AE004091 Genomic DNA Translation: AAG07945.1 |
PIRi | G83076 |
RefSeqi | NP_253247.1, NC_002516.2 WP_003112824.1, NZ_QZGE01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAG07945; AAG07945; PA4557 |
GeneIDi | 879669 |
KEGGi | pae:PA4557 |
PATRICi | fig|208964.12.peg.4769 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY273871 Genomic DNA Translation: AAP81279.1 AE004091 Genomic DNA Translation: AAG07945.1 |
PIRi | G83076 |
RefSeqi | NP_253247.1, NC_002516.2 WP_003112824.1, NZ_QZGE01000004.1 |
3D structure databases
SMRi | Q9HVM7 |
ModBasei | Search... |
Chemistry databases
BindingDBi | Q9HVM7 |
Proteomic databases
PaxDbi | Q9HVM7 |
PRIDEi | Q9HVM7 |
Protocols and materials databases
DNASUi | 879669 |
Genome annotation databases
EnsemblBacteriai | AAG07945; AAG07945; PA4557 |
GeneIDi | 879669 |
KEGGi | pae:PA4557 |
PATRICi | fig|208964.12.peg.4769 |
Organism-specific databases
PseudoCAPi | PA4557 |
Phylogenomic databases
HOGENOMi | CLU_027486_1_0_6 |
InParanoidi | Q9HVM7 |
OMAi | HNKYVVD |
PhylomeDBi | Q9HVM7 |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00097 UPA00059;UER00105 |
BioCyci | PAER208964:G1FZ6-4650-MONOMER |
Family and domain databases
CDDi | cd13944, lytB_ispH, 1 hit |
HAMAPi | MF_00191, IspH, 1 hit |
InterProi | View protein in InterPro IPR003451, LytB/IspH |
PANTHERi | PTHR30426, PTHR30426, 1 hit |
Pfami | View protein in Pfam PF02401, LYTB, 1 hit |
TIGRFAMsi | TIGR00216, ispH_lytB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPH_PSEAE | |
Accessioni | Q9HVM7Primary (citable) accession number: Q9HVM7 Secondary accession number(s): Q7WZN6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families