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Protein

Lipid A export ATP-binding/permease protein MsbA

Gene

msbA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi390 – 397ATPUniRule annotation8

GO - Molecular functioni

  • ATPase activity Source: PseudoCAP
  • ATP binding Source: UniProtKB-KW
  • ATP transmembrane transporter activity Source: PseudoCAP
  • lipid-transporting ATPase activity Source: GO_Central

GO - Biological processi

  • lipid A biosynthetic process Source: PseudoCAP
  • lipopolysaccharide transport Source: PseudoCAP

Keywordsi

Molecular functionHydrolase
Biological processLipid transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-5113-MONOMER
BRENDAi3.6.3.39 5087

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A export ATP-binding/permease protein MsbAUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:msbAUniRule annotation
Ordered Locus Names:PA4997
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4997

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei79 – 99HelicalUniRule annotationAdd BLAST21
Transmembranei161 – 178HelicalUniRule annotationAdd BLAST18
Transmembranei183 – 202HelicalUniRule annotationAdd BLAST20
Transmembranei269 – 289HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000925931 – 603Lipid A export ATP-binding/permease protein MsbAAdd BLAST603

Proteomic databases

PaxDbiQ9HUG8
PRIDEiQ9HUG8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA4997

Structurei

3D structure databases

ProteinModelPortaliQ9HUG8
SMRiQ9HUG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 324ABC transmembrane type-1UniRule annotationAdd BLAST295
Domaini356 – 592ABC transporterUniRule annotationAdd BLAST237

Domaini

In MsbA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ1 Bacteria
COG1132 LUCA
InParanoidiQ9HUG8
KOiK11085
OMAiGDLMNRI
PhylomeDBiQ9HUG8

Family and domain databases

Gene3Di1.20.1560.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR011917 ABC_transpr_lipidA
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00664 ABC_membrane, 1 hit
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF90123 SSF90123, 1 hit
TIGRFAMsiTIGR02203 MsbA_lipidA, 1 hit
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51239 MSBA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HUG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSPQNPGP SSLKIYFRLL GYVKPYIGMF LLSIVGFLIF ASTQPMLAGI
60 70 80 90 100
LKYFVDGLSN PDAALFPNVQ WPWLRDLHLV YAVPLLIILI AAWQGLGSFL
110 120 130 140 150
GNFFLAKVSL GLVHDLRVAL FNKLLVLPNR YFDTHSSGHL ISRITFNVTM
160 170 180 190 200
VTGAATDAIK VVIREGLTVV FLFLYLLWMN WKLTLVMLAI LPVIAVMVTT
210 220 230 240 250
ASRKFRKQSK KIQVAMGDVT HVASETIQGY RVVRSFGGEA YEEKRFLDAS
260 270 280 290 300
QSNTDKQLRM TKTGAVYTPM LQLVIYVAMA ILMFLVLWLR GDASAGDLVA
310 320 330 340 350
YITAAGLLPK PIRQLSEVSS TVQRGVAGAE SIFEQLDEAA EEDQGTVEKE
360 370 380 390 400
RVSGRLEVRN LSFRYPGTDK QVLDDISFIA EPGQMIALVG RSGSGKSTLA
410 420 430 440 450
NLVPRFYQHN DGKILLDGVE VEDYRLRNLR RHIALVTQQV TLFNDSVANN
460 470 480 490 500
IAYGDLAGAP REEIERAAKA ANAKEFIDNL PQGFDTEVGE NGVLLSGGQR
510 520 530 540 550
QRLAIARALL KDAPLLILDE ATSALDTESE RHIQAALDEV MKGRTTLVIA
560 570 580 590 600
HRLSTIEKAD LILVMDQGQI VERGSHAELL AQNGHYARLH AMGLDEQAPA

PVG
Length:603
Mass (Da):66,432
Last modified:March 1, 2001 - v1
Checksum:i4A91DA2845BCF5C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG08382.1
PIRiB83022
RefSeqiNP_253684.1, NC_002516.2
WP_003114544.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG08382; AAG08382; PA4997
GeneIDi881672
KEGGipae:PA4997
PATRICifig|208964.12.peg.5237

Similar proteinsi

Entry informationi

Entry nameiMSBA_PSEAE
AccessioniPrimary (citable) accession number: Q9HUG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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