UniProtKB - Q9HU22 (Q9HU22_PSEAE)
Protein
Glucose-1-phosphate thymidylyltransferase
Gene
rmlA
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.UniRule annotation
Catalytic activityi
- α-D-glucose 1-phosphate + dTTP + H+ = diphosphate + dTDP-α-D-glucoseUniRule annotationARBA annotationEC:2.7.7.24UniRule annotationARBA annotation
Cofactori
Mg2+ARBA annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 110 | TTPCombined sources | 1 | |
Binding sitei | 111 | Glucose-1-phosphateCombined sources | 1 | |
Binding sitei | 146 | Glucose-1-phosphate; via amide nitrogenCombined sources | 1 | |
Binding sitei | 172 | Glucose-1-phosphate; via carbonyl oxygenCombined sources | 1 | |
Binding sitei | 251 | TTP; via amide nitrogenCombined sources | 1 | |
Binding sitei | 255 | TTPCombined sources | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 15 | TTPCombined sources | 6 | |
Nucleotide bindingi | 25 – 26 | TTPCombined sources | 2 | |
Nucleotide bindingi | 82 – 87 | TTPCombined sources | 6 | |
Nucleotide bindingi | 114 – 119 | TTPCombined sources | 6 | |
Nucleotide bindingi | 219 – 220 | TTPCombined sources | 2 |
GO - Molecular functioni
- glucose-1-phosphate thymidylyltransferase activity Source: PseudoCAP
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- dTDP-rhamnose biosynthetic process Source: PseudoCAP
- extracellular polysaccharide biosynthetic process Source: InterPro
- lipopolysaccharide core region biosynthetic process Source: PseudoCAP
Keywordsi
Molecular function | NucleotidyltransferaseUniRule annotation, Transferase |
Ligand | MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-bindingCombined sources |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-5280-MONOMER |
BRENDAi | 2.7.7.24, 5087 |
Names & Taxonomyi
Protein namesi | Recommended name: Glucose-1-phosphate thymidylyltransferaseUniRule annotationARBA annotation (EC:2.7.7.24UniRule annotationARBA annotation) |
Gene namesi | Name:rmlAImported Ordered Locus Names:PA5163Imported |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)Imported |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA5163 |
Pathology & Biotechi
Miscellaneous databases
PHI-basei | PHI:7634 |
Chemistry databases
DrugBanki | DB03723, 2'-Deoxy-Thymidine-Beta-L-Rhamnose DB03751, 2'deoxy-Thymidine-5'-Diphospho-Alpha-D-Glucose DB02843, alpha-D-glucose-1-phosphate DB04272, Citric acid DB04485, Thymidine DB02452, Thymidine 5'-triphosphate DB01643, Thymidine monophosphate |
Structurei
3D structure databases
SMRi | Q9HU22 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9HU22 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 240 | NTP_transferaseInterPro annotationAdd BLAST | 237 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 161 – 162 | Glucose-1-phosphate bindingCombined sources | 2 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_029499_9_0_6 |
InParanoidi | Q9HU22 |
OMAi | GPYPMIY |
PhylomeDBi | Q9HU22 |
Family and domain databases
CDDi | cd02538, G1P_TT_short, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005907, G1P_thy_trans_s IPR005835, NTP_transferase_dom IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR43532, PTHR43532, 1 hit |
Pfami | View protein in Pfam PF00483, NTP_transferase, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR01207, rmlA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9HU22-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRKGIILAG GSGTRLHPAT LAISKQLLPV YDKPMIYYPL STLMLAGIRE
60 70 80 90 100
ILIISTPQDT PRFQQLLGDG SNWGLDLQYA VQPSPDGLAQ AFLIGESFIG
110 120 130 140 150
NDLSALVLGD NLYYGHDFHE LLGSASQRQT GASVFAYHVL DPERYGVVEF
160 170 180 190 200
DQGGKAISLE EKPLEPKSNY AVTGLYFYDQ QVVDIARDLK PSPRGELEIT
210 220 230 240 250
DVNRAYLERG QLSVEIMGRG YAWLDTGTHD SLLEAGQFIA TLENRQGLKV
260 270 280 290
ACPEEIAYRQ KWIDAAQLEK LAAPLAKNGY GQYLKRLLTE TVY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG08548.1 |
PIRi | D83000 |
RefSeqi | NP_253850.1, NC_002516.2 WP_003105518.1, NZ_QZGE01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAG08548; AAG08548; PA5163 |
GeneIDi | 879990 |
KEGGi | pae:PA5163 |
PATRICi | fig|208964.12.peg.5411 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG08548.1 |
PIRi | D83000 |
RefSeqi | NP_253850.1, NC_002516.2 WP_003105518.1, NZ_QZGE01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FXO | X-ray | 1.66 | A/B/C/D/E/F/G/H | 1-293 | [»] | |
1FZW | X-ray | 1.90 | A/B/C/D/E/F/G/H | 1-293 | [»] | |
1G0R | X-ray | 1.87 | A/B/C/D/E/F/G/H | 1-293 | [»] | |
1G1L | X-ray | 1.77 | A/B/C/D/E/F/G/H | 1-293 | [»] | |
1G23 | X-ray | 2.80 | A/B/C/D/E/F/G/H | 1-293 | [»] | |
1G2V | X-ray | 2.60 | A/B/C/D/E/F/G/H | 1-293 | [»] | |
1G3L | X-ray | 2.70 | A/B/C/D | 1-293 | [»] | |
3ZLK | X-ray | 1.95 | A/B/C/D | 1-293 | [»] | |
3ZLL | X-ray | 2.00 | A/B/C/D | 1-293 | [»] | |
4ARW | X-ray | 2.20 | A/B/C/D | 1-293 | [»] | |
4ASJ | X-ray | 2.25 | A/B/C/D | 1-293 | [»] | |
4ASY | X-ray | 2.30 | A/B/C/D | 1-293 | [»] | |
4B2W | X-ray | 2.36 | A/B/C/D | 1-293 | [»] | |
4B2X | X-ray | 1.70 | A/B/C/D | 1-293 | [»] | |
4B3U | X-ray | 1.80 | A/B/C/D | 1-293 | [»] | |
4B42 | X-ray | 2.50 | A/B/C/D | 1-293 | [»] | |
4B4B | X-ray | 2.10 | A/B/C/D | 1-293 | [»] | |
4B4G | X-ray | 2.50 | A/B/C/D | 1-293 | [»] | |
4B4M | X-ray | 2.35 | A/B/C/D | 1-293 | [»] | |
4B5B | X-ray | 2.06 | A/B/C/D | 1-293 | [»] | |
5FTS | X-ray | 2.20 | A/B/C/D | 1-293 | [»] | |
5FTV | X-ray | 2.21 | A/B/C/D | 1-293 | [»] | |
5FU0 | X-ray | 1.90 | A/B/C/D | 1-293 | [»] | |
5FU8 | X-ray | 2.20 | A/B/C/D | 1-293 | [»] | |
5FUH | X-ray | 1.60 | A/B/C/D | 1-293 | [»] | |
5FYE | X-ray | 2.40 | A/B/C/D | 1-293 | [»] | |
6T37 | X-ray | 2.08 | A/B/C/D | 1-293 | [»] | |
6T38 | X-ray | 2.15 | A/B/C/D | 1-293 | [»] | |
6TQG | X-ray | 2.45 | A/B/C/D | 1-293 | [»] | |
SMRi | Q9HU22 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
BindingDBi | Q9HU22 |
DrugBanki | DB03723, 2'-Deoxy-Thymidine-Beta-L-Rhamnose DB03751, 2'deoxy-Thymidine-5'-Diphospho-Alpha-D-Glucose DB02843, alpha-D-glucose-1-phosphate DB04272, Citric acid DB04485, Thymidine DB02452, Thymidine 5'-triphosphate DB01643, Thymidine monophosphate |
Proteomic databases
PaxDbi | Q9HU22 |
PRIDEi | Q9HU22 |
Genome annotation databases
EnsemblBacteriai | AAG08548; AAG08548; PA5163 |
GeneIDi | 879990 |
KEGGi | pae:PA5163 |
PATRICi | fig|208964.12.peg.5411 |
Organism-specific databases
PseudoCAPi | PA5163 |
Phylogenomic databases
HOGENOMi | CLU_029499_9_0_6 |
InParanoidi | Q9HU22 |
OMAi | GPYPMIY |
PhylomeDBi | Q9HU22 |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-5280-MONOMER |
BRENDAi | 2.7.7.24, 5087 |
Miscellaneous databases
EvolutionaryTracei | Q9HU22 |
PHI-basei | PHI:7634 |
Family and domain databases
CDDi | cd02538, G1P_TT_short, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005907, G1P_thy_trans_s IPR005835, NTP_transferase_dom IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR43532, PTHR43532, 1 hit |
Pfami | View protein in Pfam PF00483, NTP_transferase, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR01207, rmlA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q9HU22_PSEAE | |
Accessioni | Q9HU22Primary (citable) accession number: Q9HU22 Secondary accession number(s): Q7AYQ9 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | March 1, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |