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Protein

Murein tetrapeptide carboxypeptidase

Gene

PA5198

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases the terminal D-alanine residue from the cytoplasmic disaccharide-tetrapeptide GlcNAc-MurNAc-L-Ala-gamma-D-Glu-meso-Dap-D-Ala, which is a murein turnover product. Probably also act on free tetrapetide. May be involved in murein recycling.

Catalytic activityi

GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + H2O = GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + D-alanine.1 Publication

Pathwayi: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Nucleophile1 Publication1
Active sitei217Charge relay system1 Publication1
Active sitei285Charge relay system1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease, Serine protease
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-20218
PAER208964:G1FZ6-5317-MONOMER
BRENDAi3.4.17.13 5087
UniPathwayi
UPA00544

Protein family/group databases

MEROPSiS66.001

Names & Taxonomyi

Protein namesi
Recommended name:
Murein tetrapeptide carboxypeptidase (EC:3.4.17.13)
Alternative name(s):
LD-carboxypeptidase
Gene namesi
Ordered Locus Names:PA5198
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5198

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115S → A: Loss of activity. 1 Publication1
Mutagenesisi217E → A: Loss of activity. 1 Publication1
Mutagenesisi285H → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003889731 – 307Murein tetrapeptide carboxypeptidaseAdd BLAST307

Proteomic databases

PaxDbiQ9HTZ1

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA5198

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9HTZ1
SMRiQ9HTZ1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HTZ1

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S66 family.Curated

Phylogenomic databases

eggNOGiENOG4107WMG Bacteria
COG1619 LUCA
HOGENOMiHOG000025480
InParanoidiQ9HTZ1
KOiK01297
OMAiQGTPYMP
PhylomeDBiQ9HTZ1

Family and domain databases

Gene3Di3.40.50.10740, 1 hit
3.50.30.60, 1 hit
InterProiView protein in InterPro
IPR027461 Carboxypeptidase_A_C_sf
IPR029062 Class_I_gatase-like
IPR027478 LdcA_N
IPR003507 S66_fam
PANTHERiPTHR30237 PTHR30237, 1 hit
PfamiView protein in Pfam
PF02016 Peptidase_S66, 1 hit
PIRSFiPIRSF028757 LD-carboxypeptidase, 1 hit
SUPFAMiSSF141986 SSF141986, 1 hit
SSF52317 SSF52317, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HTZ1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSRPSSDQT WQPIDGRVAL IAPASAIATD VLEATLRQLE VHGVDYHLGR
60 70 80 90 100
HVEARYRYLA GTVEQRLEDL HNAFDMPDIT AVWCLRGGYG CGQLLPGLDW
110 120 130 140 150
GRLQAASPRP LIGFSDISVL LSAFHRHGLP AIHGPVATGL GLSPLSAPRE
160 170 180 190 200
QQERLASLAS VSRLLAGIDH ELPVQHLGGH KQRVEGALIG GNLTALACMA
210 220 230 240 250
GTLGGLHAPA GSILVLEDVG EPYYRLERSL WQLLESIDAR QLGAICLGSF
260 270 280 290 300
TDCPRKEVAH SLERIFGEYA AAIEVPLYHH LPSGHGAQNR AWPYGKTAVL

EGNRLRW
Length:307
Mass (Da):33,288
Last modified:March 1, 2001 - v1
Checksum:i0E6BA75C30DC8C9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG08583.1
PIRiE82997
RefSeqiNP_253885.1, NC_002516.2
WP_003100070.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG08583; AAG08583; PA5198
GeneIDi880289
KEGGipae:PA5198
PATRICifig|208964.12.peg.5448

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG08583.1
PIRiE82997
RefSeqiNP_253885.1, NC_002516.2
WP_003100070.1, NC_002516.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZL0X-ray1.10A/B1-307[»]
1ZRSX-ray1.50A/B1-307[»]
2AUMX-ray2.40A/B1-307[»]
2AUNX-ray2.40A/B1-307[»]
ProteinModelPortaliQ9HTZ1
SMRiQ9HTZ1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5198

Protein family/group databases

MEROPSiS66.001

Proteomic databases

PaxDbiQ9HTZ1

Protocols and materials databases

DNASUi880289
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08583; AAG08583; PA5198
GeneIDi880289
KEGGipae:PA5198
PATRICifig|208964.12.peg.5448

Organism-specific databases

PseudoCAPiPA5198

Phylogenomic databases

eggNOGiENOG4107WMG Bacteria
COG1619 LUCA
HOGENOMiHOG000025480
InParanoidiQ9HTZ1
KOiK01297
OMAiQGTPYMP
PhylomeDBiQ9HTZ1

Enzyme and pathway databases

UniPathwayi
UPA00544

BioCyciMetaCyc:MONOMER-20218
PAER208964:G1FZ6-5317-MONOMER
BRENDAi3.4.17.13 5087

Miscellaneous databases

EvolutionaryTraceiQ9HTZ1

Family and domain databases

Gene3Di3.40.50.10740, 1 hit
3.50.30.60, 1 hit
InterProiView protein in InterPro
IPR027461 Carboxypeptidase_A_C_sf
IPR029062 Class_I_gatase-like
IPR027478 LdcA_N
IPR003507 S66_fam
PANTHERiPTHR30237 PTHR30237, 1 hit
PfamiView protein in Pfam
PF02016 Peptidase_S66, 1 hit
PIRSFiPIRSF028757 LD-carboxypeptidase, 1 hit
SUPFAMiSSF141986 SSF141986, 1 hit
SSF52317 SSF52317, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLDC_PSEAE
AccessioniPrimary (citable) accession number: Q9HTZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2001
Last modified: February 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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