UniProtKB - Q9HRW3 (RAD50_HALSA)
Protein
DNA double-strand break repair Rad50 ATPase
Gene
rad50
Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Functioni
Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per homodimer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | ATPUniRule annotation | 1 | |
Binding sitei | 137 | ATPUniRule annotation | 1 | |
Metal bindingi | 454 | ZincUniRule annotation | 1 | |
Metal bindingi | 457 | ZincUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 32 – 38 | ATPUniRule annotation | 7 |
GO - Molecular functioni
- ATPase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- double-strand break repair Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: DNA double-strand break repair Rad50 ATPaseUniRule annotation |
Gene namesi | Name:rad50UniRule annotation Ordered Locus Names:VNG_0514C |
Organismi | Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) |
Taxonomic identifieri | 64091 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Stenosarchaea group › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutants show a slight growth defect, but do not show increased sensitivity to ionizing radiation or alkylating agents. Show decreased rates of survival after UV-C irradiation.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000138651 | 1 – 883 | DNA double-strand break repair Rad50 ATPaseAdd BLAST | 883 |
Proteomic databases
PaxDbi | Q9HRW3 |
PRIDEi | Q9HRW3 |
Interactioni
Subunit structurei
Homodimer.
Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 407 – 506 | Zinc-hookUniRule annotationAdd BLAST | 100 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 244 – 283 | UniRule annotationAdd BLAST | 40 | |
Coiled coili | 336 – 389 | UniRule annotationAdd BLAST | 54 | |
Coiled coili | 414 – 452 | UniRule annotationAdd BLAST | 39 | |
Coiled coili | 571 – 604 | UniRule annotationAdd BLAST | 34 | |
Coiled coili | 668 – 720 | UniRule annotationAdd BLAST | 53 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 782 – 825 | Ala/Asp-rich (DA-box)Add BLAST | 44 |
Domaini
The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.UniRule annotation
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
HOGENOMi | CLU_004785_0_1_2 |
InParanoidi | Q9HRW3 |
OMAi | LRCAIYR |
OrthoDBi | 1771at2157 |
Family and domain databases
HAMAPi | MF_00449, RAD50, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR038729, Rad50/SbcC_AAA IPR022982, Rad50_ATPase_archaeal IPR013134, Zn_hook_RAD50 |
Pfami | View protein in Pfam PF13476, AAA_23, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51131, ZN_HOOK, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9HRW3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRFTRLSLSN FKCYADAAVS LDPGVTVIHG LNGSGKSSLL DACFFALYGT
60 70 80 90 100
TALDTTLADA VTIGAETAEI DLHFEHAGGD YHVHRRIRAS GGRAQTAACV
110 120 130 140 150
LETPTDRIDG VTDVEAHISG LLRMDAEAFV NCAYVRQGEV NKLINAAPST
160 170 180 190 200
RQDMIDALLQ LGKLEEYRQR AGDARLGVED VKSNVEGQLD RLADQIADKE
210 220 230 240 250
AADPHDRLAS HNTALAEVTA DIEHFEAERE QARQTRDDAA DVLERYEESR
260 270 280 290 300
TALADVEETI ADVREAVAEA ERERETLADR VSDHRERASD LDDEAAALAA
310 320 330 340 350
DLGLDDPDAE DASAERDAVA DQREAVAERV REVAPAVSRL TEQADSAADD
360 370 380 390 400
AATLDERAET LREEAAALDA EADDAAAKRD DAAARIEALD ADIEAAMAAF
410 420 430 440 450
DDAPVAFGAA EAFLDDATAE RDELRERVAT LRADRQSAAD RVAEAEALLD
460 470 480 490 500
EGKCPECGQP VEGAPHVERV TDDRERVAEL DAELADVEDE LDAVAQRVDR
510 520 530 540 550
GESLVAAEDR VDDLEQQRER AVERRDEQAD IADAKRDQAA EKRDRAADLD
560 570 580 590 600
AEAEDARADA AAKRDAADEK RETLAALNAD QTALKERLDA LADLVDRLEA
610 620 630 640 650
AADAREAAQR LAEKRAALAA QNEQRRDRLS ELRERKRTLD SEFDADRIET
660 670 680 690 700
ARADKDRAED YLEQVEPKLQ ALREDRDDLQ AKIGAAENAI AELESLREEH
710 720 730 740 750
ERVQSRHQDL QAVHDEVTAL ETMYGELRAE LRQQNVSKLE RLLNETFELV
760 770 780 790 800
YQNDSYARIE LSGEYELTVY QKDGEPLEPA QLSGGERALF NLSLRTAVYR
810 820 830 840 850
LLAEGIEGDA PLPPLILDEP TVFLDSGHVS QLVELVESMR RLGVEQIVVV
860 870 880
SHDDELVAAA DDVVRVAKDA TSNRSRVSTP EHI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004437 Genomic DNA Translation: AAG19045.1 |
PIRi | A84210 |
RefSeqi | WP_010902341.1, NC_002607.1 |
Genome annotation databases
EnsemblBacteriai | AAG19045; AAG19045; VNG_0514C |
GeneIDi | 5952569 |
KEGGi | hal:VNG_0514C |
PATRICi | fig|64091.14.peg.392 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004437 Genomic DNA Translation: AAG19045.1 |
PIRi | A84210 |
RefSeqi | WP_010902341.1, NC_002607.1 |
3D structure databases
SMRi | Q9HRW3 |
ModBasei | Search... |
Proteomic databases
PaxDbi | Q9HRW3 |
PRIDEi | Q9HRW3 |
Genome annotation databases
EnsemblBacteriai | AAG19045; AAG19045; VNG_0514C |
GeneIDi | 5952569 |
KEGGi | hal:VNG_0514C |
PATRICi | fig|64091.14.peg.392 |
Phylogenomic databases
HOGENOMi | CLU_004785_0_1_2 |
InParanoidi | Q9HRW3 |
OMAi | LRCAIYR |
OrthoDBi | 1771at2157 |
Family and domain databases
HAMAPi | MF_00449, RAD50, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR038729, Rad50/SbcC_AAA IPR022982, Rad50_ATPase_archaeal IPR013134, Zn_hook_RAD50 |
Pfami | View protein in Pfam PF13476, AAA_23, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51131, ZN_HOOK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD50_HALSA | |
Accessioni | Q9HRW3Primary (citable) accession number: Q9HRW3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2004 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families