UniProtKB - Q9HLA5 (KAE1B_THEAC)
Protein
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Gene
Ta0324
Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Functioni
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation
Catalytic activityi
- EC:2.7.11.1UniRule annotation
- EC:2.7.11.1UniRule annotation
- adenosine37 in tRNA + L-threonylcarbamoyladenylate = AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNAUniRule annotationEC:2.3.1.234UniRule annotation
Cofactori
Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 107 | IronUniRule annotation | 1 | |
Metal bindingi | 111 | IronUniRule annotation | 1 | |
Metal bindingi | 128 | IronUniRule annotation | 1 | |
Binding sitei | 160 | Threonylcarbamoyl-AMPUniRule annotation | 1 | |
Binding sitei | 173 | Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 177 | Threonylcarbamoyl-AMPUniRule annotation | 1 | |
Binding sitei | 257 | Threonylcarbamoyl-AMPUniRule annotation | 1 | |
Metal bindingi | 285 | IronUniRule annotation | 1 | |
Binding sitei | 355 | ATPUniRule annotation | 1 | |
Active sitei | 447 | Proton acceptor; for kinase activityUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 335 – 342 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- iron ion binding Source: UniProtKB-UniRule
- metalloendopeptidase activity Source: InterPro
- N(6)-L-threonylcarbamoyladenine synthase activity Source: UniProtKB-EC
- protein serine/threonine/tyrosine kinase activity Source: UniProtKB-UniRule
- protein serine/threonine kinase activity Source: UniProtKB-KW
- zinc ion binding Source: InterPro
GO - Biological processi
- tRNA threonylcarbamoyladenosine modification Source: UniProtKB-UniRule
Keywordsi
Molecular function | Acyltransferase, Kinase, Multifunctional enzyme, Serine/threonine-protein kinase, Transferase |
Biological process | tRNA processing |
Ligand | ATP-binding, Iron, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | TACI273075:G1GT7-342-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotationIncluding the following 2 domains: Alternative name(s): N6-L-threonylcarbamoyladenine synthase Short name: t(6)A synthase t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation |
Gene namesi | Ordered Locus Names:Ta0324 |
Organismi | Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) |
Taxonomic identifieri | 273075 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Diaforarchaea group › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
- EKC/KEOPS complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000303661 | 1 – 529 | Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinAdd BLAST | 529 |
Proteomic databases
PRIDEi | Q9HLA5 |
Interactioni
Subunit structurei
Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.
UniRule annotationProtein-protein interaction databases
STRINGi | 273075.Ta0324 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9HLA5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9HLA5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 329 – 529 | Protein kinaseUniRule annotationAdd BLAST | 201 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 324 | Kae1Add BLAST | 324 | |
Regioni | 128 – 132 | Threonylcarbamoyl-AMP bindingUniRule annotation | 5 |
Sequence similaritiesi
In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG01183 Archaea arCOG01185 Archaea COG0533 LUCA COG3642 LUCA |
HOGENOMi | HOG000109569 |
KOi | K15904 |
OMAi | GETMDTG |
OrthoDBi | 9881at2157 |
Family and domain databases
HAMAPi | MF_01446 Kae1, 1 hit MF_01447 Kae1_Bud32_arch, 1 hit |
InterProi | View protein in InterPro IPR022495 Bud32 IPR000905 Gcp-like_dom IPR034680 Kae1/OSGEP IPR017861 KAE1/TsaD IPR022449 Kae1_arc IPR011009 Kinase-like_dom_sf IPR009220 tRNA_threonyl_synthase/kinase |
PANTHERi | PTHR11735 PTHR11735, 1 hit PTHR11735:SF14 PTHR11735:SF14, 1 hit |
Pfami | View protein in Pfam PF00814 Peptidase_M22, 1 hit |
PIRSFi | PIRSF036401 Gcp_STYKS, 1 hit |
PRINTSi | PR00789 OSIALOPTASE |
SUPFAMi | SSF56112 SSF56112, 1 hit |
TIGRFAMsi | TIGR03724 arch_bud32, 1 hit TIGR03722 arch_KAE1, 1 hit TIGR00329 gcp_kae1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9HLA5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVLGLEGTA HTISCGIIDE SRILAMESSM YRPKTGGIRP LDAAVHHSEV
60 70 80 90 100
IDTVISRALE KAKISIHDID LIGFSMGPGL APSLRVTATA ARTISVLTGK
110 120 130 140 150
PIIGVNHPLG HIEIGRRVTG AIDPVMLYVS GGNTQVIAHV NGRYRVLGET
160 170 180 190 200
LDIGIGNMID KFAREAGIPF PGGPEIEKLA MKGTKLLDLP YSVKGMDTAF
210 220 230 240 250
SGILTAALQY LKTGQAIEDI SYSIQETAFA MLVEVLERAL YVSGKDEILM
260 270 280 290 300
AGGVALNRRL RDMVTNMARE AGIRSYLTDR EYCMDNGIMI AQAALLMYKS
310 320 330 340 350
GVRMSVEETA VNPRFRIDEV DAPWITDASR KDYGKAGAES RIEEVSFHGR
360 370 380 390 400
PAIRKVRISK SYRNSDLDKK IRYERMRNEF TILRKLKEAG VNSPVVYDFD
410 420 430 440 450
PFSMSITMQK IPGRMMSAEL NEGRTDFLNE LGIMIAKMHR AGIAHGDLTV
460 470 480 490 500
NNIIVNDSVF IIDPSMGKVN AEIEDMAVDI YALEDSIKGL GLDSGSVIGQ
510 520
MLKSYRNNFN LADDVLETVS AIRRRHRYV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL445064 Genomic DNA Translation: CAC11469.1 |
RefSeqi | WP_010900753.1, NC_002578.1 |
Genome annotation databases
EnsemblBacteriai | CAC11469; CAC11469; CAC11469 |
GeneIDi | 1455944 |
KEGGi | tac:Ta0324 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL445064 Genomic DNA Translation: CAC11469.1 |
RefSeqi | WP_010900753.1, NC_002578.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ENO | X-ray | 3.02 | A/B | 1-329 | [»] | |
SMRi | Q9HLA5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 273075.Ta0324 |
Proteomic databases
PRIDEi | Q9HLA5 |
Genome annotation databases
EnsemblBacteriai | CAC11469; CAC11469; CAC11469 |
GeneIDi | 1455944 |
KEGGi | tac:Ta0324 |
Phylogenomic databases
eggNOGi | arCOG01183 Archaea arCOG01185 Archaea COG0533 LUCA COG3642 LUCA |
HOGENOMi | HOG000109569 |
KOi | K15904 |
OMAi | GETMDTG |
OrthoDBi | 9881at2157 |
Enzyme and pathway databases
BioCyci | TACI273075:G1GT7-342-MONOMER |
Miscellaneous databases
EvolutionaryTracei | Q9HLA5 |
Family and domain databases
HAMAPi | MF_01446 Kae1, 1 hit MF_01447 Kae1_Bud32_arch, 1 hit |
InterProi | View protein in InterPro IPR022495 Bud32 IPR000905 Gcp-like_dom IPR034680 Kae1/OSGEP IPR017861 KAE1/TsaD IPR022449 Kae1_arc IPR011009 Kinase-like_dom_sf IPR009220 tRNA_threonyl_synthase/kinase |
PANTHERi | PTHR11735 PTHR11735, 1 hit PTHR11735:SF14 PTHR11735:SF14, 1 hit |
Pfami | View protein in Pfam PF00814 Peptidase_M22, 1 hit |
PIRSFi | PIRSF036401 Gcp_STYKS, 1 hit |
PRINTSi | PR00789 OSIALOPTASE |
SUPFAMi | SSF56112 SSF56112, 1 hit |
TIGRFAMsi | TIGR03724 arch_bud32, 1 hit TIGR03722 arch_KAE1, 1 hit TIGR00329 gcp_kae1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAE1B_THEAC | |
Accessioni | Q9HLA5Primary (citable) accession number: Q9HLA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 8, 2019 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references